GenBank-Updates@genbank.bio.net (05/10/91)
LOCUS SYNMOV2 6829 bp ds-DNA SYN 10-MAY-1991
DEFINITION Moloney murine leukemia virus retroviral vector pLHDCX, complete
sequence.
ACCESSION M64754
KEYWORDS cloning vector; histidinol dehydrogenase; retroviral vector.
SOURCE Synthetic, DNA.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 6829)
AUTHORS Stockshlaeder,M.A., Storb,R., Osborne,W.R. and Miller,A.D.
TITLE L-histidinol provides effective selection of
retrovirus-vector-transduced keratinocytes without impairing their
proliferative potential
JOURNAL Hum. Gene Ther. 2, 33-39 (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
misc_recomb 144^145
/note="mouse DNA end/Mo-MuSV DNA start"
LTR 145..733
/note="Mo-MuSV 5' long terminal repeat"
misc_signal 803..1604
/note="extended packaging signal"
misc_recomb 1129^1130
/note="Mo-MuSV DNA end/Mo-MuLV DNA start"
mutation 1185..1187
/note="gag ATG start codon to TAG stop codon"
misc_recomb 1654^1655
/note="Mo-MuLV DNA end/hisD DNA start"
CDS 1664..2968
/EC_number="1.1.1.23"
/product="histidinol dehydrogenase"
/codon_start=1664
misc_recomb 3008^3009
/note="hisD DNA end/human cytomegalavirus start"
promoter 3009..3826
/note="human cytomegalovirus early promoter"
misc_recomb 3826^3827
/note="human cytomegalovirus DNA end/Mo-MuLV DNA start"
LTR 3875..4468
/note="Mo-MuLV 3' long terminal repeat"
misc_recomb 4537^4538
/note="Mo-MuLV DNA end/plasmid pBR322 DNA start"
BASE COUNT 1608 a 1874 c 1720 g 1627 t
ORIGIN
1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
241 atacataact gagaatagga aagttcagat caaggtcagg aacaaagaaa cagctgaata
301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
1141 ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt
1201 atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact
1261 ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg
1321 ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct
1381 ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac
1441 acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct
1501 gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc
1561 tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc
1621 cttctctagg cgccggaatt cgttaactcg aggatcccgg accatgagct tcaataccct
1681 gattgactgg aacagctgta gccctgaaca gcagcgtgcg ctgctgacgc gtccggcgat
1741 ttccgcctct gacagtatta cccggacggt cagcgatatt ctggataatg taaaaacgcg
1801 cggtgacgat gccctgcgtg aatacagcgc taaatttgat aaaacagaag tgacagcgct
1861 acgcgtcacc cctgaagaga tcgccgccgc cggcgcgcgt ctgagcgacg aattaaaaca
1921 ggcgatgacc gctgccgtca aaaatattga aacgttccat tccgcgcaga cgctaccgcc
1981 tgtagatgtg gaaacccagc caggcgtgcg ttgccagcag gttacgcgtc ccgtctcgtc
2041 tgtcggtctg tatattcccg gcggctcggc tccgctcttc tcaacggtgc tgatgctggc
2101 gacgccggcg cgcattgcgg gatgccagaa ggtggttctg tgctcgccgc cgcccatcgc
2161 tgatgaaatc ctctatgcgg cgcaactgtg tggcgtgcag gaaatcttta acgtcggcgg
2221 cgcgcaggcg attgccgctc tggccttcgg cagcgagtcc gtaccgaaag tggataaaat
2281 ttttggcccc ggcaacgcct ttgtaaccga agccaaacgt caggtcagcc agcgtctcga
2341 cggcgcggct atcgatatgc cagccgggcc gtctgaagta ctggtgatcg cagacagcgg
2401 cgcaacaccg gatttcgtcg cttctgacct gctctcccag gctgagcacg gcccggattc
2461 ccaggtgatc ctgctgacgc ctgatgctga cattgcccgc aaggtggcgg aggcggtaga
2521 acgtcaactg gcggaactgc cgcgcgcgga caccgcccgg caggccctga gcgccagtcg
2581 tctgattgtg accaaagatt tagcgcagtg cgtcgccatc tctaatcagt atgggccgga
2641 acacttaatc atccagacgc gcaatgcgcg cgatttggtg gatgcgatta ccagcgcagg
2701 ctcggtattt ctcggcgact ggtcgccgga atccgccggt gattacgctt ccggaaccaa
2761 ccatgtttta ccgacctatg gctatactgc tacctgttcc agccttgggt tagcggattt
2821 ccagaaacgg atgaccgttc aggaactgtc gaaagcgggc ttttccgctc tggcatcaac
2881 cattgaaaca ttggcggcgg cagaacgtct gaccgcccat aaaaatgccg tgaccctgcg
2941 cgtaaacgcc ctcaaggagc aagcatgagc actgaaaaca ctctcagcgt cgctgactta
3001 gcccgggatc cggccattag ccatattatt cattggttat atagcataaa tcaatattgg
3061 ctattggcca ttgcatacgt tgtatccata tcataatatg tacatttata ttggctcatg
3121 tccaacatta ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
3181 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
3241 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
3301 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
3361 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
3421 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
3481 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
3541 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
3601 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
3661 ctccgcccca ttgacgcaaa tgggcggtag gcatgtacgg tgggaggtct atataagcag
3721 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
3781 tagaagacac cgggaccgat ccagcctccg cggccccaag cttgttaaca tcgataaaat
3841 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
3901 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag
3961 agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat
4021 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg
4081 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
4141 gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt
4201 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
4261 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
4321 ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc
4381 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
4441 gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc
4501 ccagggacca ccgacccacc accgggaggt aagctggctg cctcgcgcgt ttcggtgatg
4561 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
4621 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
4681 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
4741 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
4801 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
4861 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
4921 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
4981 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
5041 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
5101 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
5161 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
5221 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
5281 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
5341 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
5401 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
5461 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
5521 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
5581 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
5641 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
5701 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
5761 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
5821 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
5881 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
5941 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
6001 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
6061 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
6121 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
6181 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
6241 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
6301 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
6361 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
6421 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
6481 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
6541 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
6601 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
6661 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
6721 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
6781 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaa
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