GenBank-Updates@genbank.bio.net (05/11/91)
LOCUS RATNGF 3935 bp ds-DNA ROD 11-MAY-1991
DEFINITION Rat nerve growth factor (NGF) gene, complete cds.
ACCESSION M60522
KEYWORDS nerve growth factor.
SOURCE Rat DNA (library of Bonner).
ORGANISM Rattus norvegicus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 3935)
AUTHORS Salton,S.R.J., Fischberg,D.J. and Dong,K.-W.
TITLE Structure of the gene encoding vgf, a nervous system-specific mRNA
that is rapidly and selectively induced by nerve growth factor in
pc12 cells
JOURNAL Mol. Cell. Biol. 11, 2335-2349 (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
mRNA join(854..1032,1295..1410,1535..3875)
/product="nerve growth factor"
/gene="NGF"
GC_signal 242..247
/gene="NGF"
GC_signal 596..601
/gene="NGF"
CAAT_signal 718..721
/gene="NGF"
GC_signal 750..755
/gene="NGF"
misc_feature 775..784
/note="CREB site"
/gene="NGF"
GC_signal 787..792
/gene="NGF"
TATA_signal 825..830
/gene="NGF"
exon 854..1032
/number=1
/gene="NGF"
intron 1033..1294
/number=1
/gene="NGF"
exon 1295..1410
/number=2
/gene="NGF"
intron 1411..1534
/number=2
/gene="NGF"
exon 1535..3875
/number=3
/gene="NGF"
CDS 1557..3410
/product="nerve growth factor"
/gene="NGF"
/codon_start=1557
misc_signal 3724..3728
/note="attta destabilization signal"
/gene="NGF"
polyA_signal 3858..3863
/gene="NGF"
BASE COUNT 761 a 1288 c 1123 g 763 t
ORIGIN
1 aagcttaagg gtgtgggaga gttggattaa aagggggcac aacagggacc ccttatcaac
61 cacagcaaag tccgctggcc agaagggcag acacaccttt tttccccacc cccttcagaa
121 ctggtttgaa cagacaggac ccagaggcac acgtatcgtc aatctttccc ttttctcttc
181 cgactaggac cctttccaag gtgattaccc aaaccgcaag atccacccat ctgcgctgac
241 cctgggcggg catcccacac cgcagatcca ccccctcttc ctctcaggtt caagtcgtcc
301 ttgggcgtgc tccctataga ccatactgtg gcggagtagg gaggcaggcc ccttccccgg
361 ctttcaatcc cttaccgtcc ccccatccag tactgatctt ttactggcgc ccaattagat
421 gctggcggtg ccctcttcct attctgctca gcaacgcggg ctccgcgcct caagagcttt
481 gtaacccacc ctcccgcctt ctggctcctc tgtacccacc cttccattca tttttccatt
541 cattcattca tccttttctc ctcgtccctc cttcattcat tcatagcccc ctgccccgcc
601 cgcctagaca tttcattcat tcattcattc attcatttcc cggagctccg ctagctcacg
661 cccctctagc cgagggccct actgcgcagg tgcaggccgg gagcgacgct tatcctccaa
721 tcattggact tccacagtcc accaggcacc cgcccgcttc cccatgaatg aacattgacg
781 tcaatggggc ggggcgagac cacgtgaccc cgcgcgctcc cctttataaa gcagcggtgg
841 cgcgggcgct gtccagcgtg ctgaagccgg agcgagagcg ctgttgctga cccagctgag
901 cccagctcct aggacgccag ccctcgacca tctttcatac tccagccacg gaacggagcc
961 agggcagacg ggtccggatt ttccccctgc cccgaccctc ctctccacct cccgccgtcg
1021 tgacaccggc tggtaaatac tccgctgtcc atccctctaa ccctcttcac tcgtgggcgt
1081 gaaaggagga ttttctttcc ttatggggta ttgagagcat agcggtgtgc ccccgggaag
1141 ggtctttggt aaacggggag tctctgcttc tctgtcgctg atgaagtcgc ccacgcacca
1201 tccggggagt cctgtgggga gggaacagat cccccctccc acttatcatt gctgcttagt
1261 acacgggcgc tggctgagag atgactttgg gaagggggcg acaggaggca ctgggtaccc
1321 agaacgagga ttgcgagcgt tctctgacca tttgcacgac cccagagatt gccacatctt
1381 tcttgttctc tgctaaacgt ttctcttcgg gtaaggttag agaaatgagg gaaaccccgg
1441 atttcctgct taaaggtggg gggagggaat cgagaaatag aaagagggaa attcttatag
1501 aaatcctcta tttctctggt ttcttctgtt tttcagtctc tggcagcccg ttggtcatga
1561 aaaccttcac gttgccagca tccgtcctct tctgcttcct tctactcatc cgggggttgg
1621 gagcagcacc ccccgggcgc tccgatgttt atcctcctcc cctcggctct gagcataatg
1681 ggcaggtagc tgaggacgca gtgtcccggc caaaggatga cagcgtccca gaggtccgag
1741 cggctcggaa ttccgagcct caggaccagg gagagctctt ccagggcgtg gatccccggg
1801 cgctggccgc ggtactgttg caggcactgg accgtccggc ctcgcccccg gctgtcccgg
1861 caggttccca gcagggaaca cccgaagaag cagcagaagc tctgctgacc gagtccgtgc
1921 gcagtcagac ccatagcctc ccggcatcag aaatccaagc gtccgctgtg gcgccccctc
1981 gccctcagac tcaggacaac gatcccgagg cagacgaccg ctcagaagag ctggaggcac
2041 tagcatcctt gctccaagaa cttcgagatt tcagtccgag taatgctaag cgccagcaag
2101 agacggcggc agcagagact gaaacccgca cgcacacgct gacccgagtc aatctggaga
2161 gccccgggcc agagcgcgta tggcgcgctt cctggggaga gttccaggcg cgcgtcccgg
2221 agcgtgctcc tctgccgccc tcggtccctt ctcaattcca ggctcgaatg tccgaaaacg
2281 ttccccttcc cgaaacccat cagttcgggg aaggagtgtc ctcccctaaa acacatcttg
2341 gtgagacttt gacaccctta tccaaggcgt accaaagtct aagtgccccc ttccccaagg
2401 tgcgtcggct cgagggctca ttcctgggcg gttccgaggc aggagagcgc ctgcttcaac
2461 aagggttagc tcaggtagag gcagggagga ggcaggcgga ggccacccgg caggccgcag
2521 cgcaagaaga gcggctggcc gatctcgcct ccgacctgct gctccagtat ttgctgcagg
2581 gcggcgcccg gcagcgcgat ctcgggggtc gcgggctgca ggagacgcag caagagcggg
2641 agaacgagag ggaggaggag gcggagcagg agagacgcgg tggtggggag gacgaggtgg
2701 gggaagagga tgaggaggcg gcagaggcgg aggcggaggc agaggaggcg gagagggcgc
2761 ggcagaacgc gctcctgttc gccgaggagg aggacgggga agccggagcc gaggacaagc
2821 gctcccagga ggaggcgcca ggccatcggc ggaaggatgc tgaggggaca gaggagggcg
2881 gggaggagga tgacgacgac gaagagatgg atccgcagac gatcgatagt ctcattgaac
2941 tgtccaccaa actccacctg ccagcagacg atgtggtcag catcatcgaa gaggtggagg
3001 agaaacggaa gcggaagaag aacgcccctc ccgagccggt gccgcccccc agggctgccc
3061 cagccccgac ccatgtccgc tccccgcagc ccccacctcc cgccccggcc cgggatgagt
3121 tgccggactg gaacgaagta ctcccaccct gggatcggga ggaggatgag gtgtttcccc
3181 cggggcccta tcaccccttc ccaaactaca ttcggccgcg gacactgcag ccgcccgcat
3241 cctcccgccg ccgtcacttc catcacgcgt tgccacctgc gcgccaccat cccgatctgg
3301 aggcccaggc caggcgcgcg caggaggaag cggacgcgga ggagcgccgg ctgcaggagc
3361 aggaggagct ggagaattac attgagcacg tgctgctgca ccgcccgtga cccgcccctg
3421 cgcgcccgct cccaactgcg cgcgccgcca cgccccccct ccgtgtcgct cctcctccct
3481 ctcggtgttt gcatgcgccc cggctccgcc cctcggctgc cgcccggccc cgccccacaa
3541 ggccccgccc cgggttctgt caggaccaga cctgtcagac ttctttgggg tctgatcctg
3601 gggccagccc aggcgggtgt gtggtttgtg cgagtcccct tacaccccca cttcctccag
3661 gggcctcgtc cccatctagt ttctctagcg acttcctggt cccaaacggg gaaaagctgt
3721 tctatttaat cgtgtgaagt gtctgtctcc cagccttggg gcccccggag cctcccttct
3781 ccaaattgct gtgaacttac ccacatcttg cccttctgtt gtaaataccc ctcacggagg
3841 aaatagtttt gctaagaaat aaaagtgact attttattag gactttgttt tccattattc
3901 acccgtcccc ttgggcctat ctggcttcaa ggggc
//GenBank-Updates@genbank.bio.net (05/11/91)
LOCUS RATNGFA 2556 bp ss-mRNA ROD 11-MAY-1991
DEFINITION Rat nerve growth factor (NGF) mRNA, complete cds.
ACCESSION M60525
KEYWORDS nerve growth factor.
SOURCE Rat cell line PC12, cDNA to mRNA.
ORGANISM Rattus norvegicus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 2556)
AUTHORS Salton,S.R.J., Fischberg,D.J. and Dong,K.-W.
TITLE Structure of the gene encoding vgf, a nervous system-specific mRNA
that is rapidly and selectively induced by nerve growth factor in
pc12 cells
JOURNAL Mol. Cell. Biol. 11, 2335-2349 (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 238..2091
/product="nerve growth factor"
/gene="NGF"
/codon_start=238
polyA_signal 2539..2544
/gene="NGF"
BASE COUNT 488 a 850 c 792 g 426 t
ORIGIN
1 ctttcatact ccagccacgg aacggagcca gggcagacgg gtccggattt tccccctgcc
61 ccgaccctcc tctccacctc ccgccgtcgt gacaccggct gggggcgaca ggaggcactg
121 ggtacccaga acgaggattg cgagcgttct ctgaccattt gcacgacccc agagattgcc
181 acatctttct tgttctctgc taaacgtttc tcttcggtct ctggcagccc gttggtcatg
241 aaaaccttca cgttgccagc atccgtcctc ttctgcttcc ttctactcat ccgggggttg
301 ggagcagcac cccccgggcg ctccgatgtt tatcctcctc ccctcggctc tgagcataat
361 gggcaggtag ctgaggacgc agtgtcccgg ccaaaggatg acagcgtccc agaggtccga
421 gcggctcgga attccgagcc tcaggaccag ggagagctct tccagggcgt ggatccccgg
481 gcgctggccg cggtactgtt gcaggcactg gaccgtccgg cctcgccccc ggctgtcccg
541 gcaggttccc agcagggaac acccgaagaa gcagcagaag ctctgctgac cgagtccgtg
601 cgcagtcaga cccatagcct cccggcatca gaaatccaag cgtccgctgt ggcgccccct
661 cgccctcaga ctcaggacaa cgatcccgag gcagacgacc gctcagaaga gctggaggca
721 ctagcatcct tgctccaaga acttcgagat ttcagtccga gtaatgctaa gcgccagcaa
781 gagacggcgg cagcagagac tgaaacccgc acgcacacgc tgacccgagt caatctggag
841 agccccgggc cagagcgcgt atggcgcgct tcctggggag agttccaggc gcgcgtcccg
901 gagcgtgctc ctctgccgcc ctcggtccct tctcaattcc aggctcgaat gtccgaaaac
961 gttccccttc ccgaaaccca tcagttcggg gaaggagtgt cctcccctaa aacacatctt
1021 ggtgagactt tgacaccctt atccaaggcg taccaaagtc taagtgcccc cttccccaag
1081 gtgcgtcggc tcgagggctc attcctgggc ggttccgagg caggagagcg cctgcttcaa
1141 caagggttag ctcaggtaga ggcagggagg aggcaggcgg aggccacccg gcaggccgca
1201 gcgcaagaag agcggctggc cgatctcgcc tccgacctgc tgctccagta tttgctgcag
1261 ggcggcgccc ggcagcgcga tctcgggggt cgcgggctgc aggagacgca gcaagagcgg
1321 gagaacgaga gggaggagga ggcggagcag gagagacgcg gtggtgggga ggacgaggtg
1381 ggggaagagg atgaggaggc ggcagaggcg gaggcggagg cagaggaggc ggagagggcg
1441 cggcagaacg cgctcctgtt cgccgaggag gaggacgggg aagccggagc cgaggacaag
1501 cgctcccagg aggaggcgcc aggccatcgg cggaaggatg ctgaggggac agaggagggc
1561 ggggaggagg atgacgacga cgaagagatg gatccgcaga cgatcgatag tctcattgaa
1621 ctgtccacca aactccacct gccagcagac gatgtggtca gcatcatcga agaggtggag
1681 gagaaacgga agcggaagaa gaacgcccct cccgagccgg tgccgccccc cagggctgcc
1741 ccagccccga cccatgtccg ctccccgcag cccccacctc ccgccccggc ccgggatgag
1801 ttgccggact ggaacgaagt actcccaccc tgggatcggg aggaggatga ggtgtttccc
1861 ccggggccct atcacccctt cccaaactac attcggccgc ggacactgca gccgcccgca
1921 tcctcccgcc gccgtcactt ccatcacgcg ttgccacctg cgcgccacca tcccgatctg
1981 gaggcccagg ccaggcgcgc gcaggaggaa gcggacgcgg aggagcgccg gctgcaggag
2041 caggaggagc tggagaatta cattgagcac gtgctgctgc accgcccgtg acccgcccct
2101 gcgcgcccgc tcccaactgc gcgcgccgcc acgccccccc tccgtgtcgc tcctcctccc
2161 tctcggtgtt tgcatgcgcc ccggctccgc ccctcggctg ccgcccggcc ccgccccaca
2221 aggccccgcc ccgggttctg tcaggaccag acctgtcaga cttctttggg gtctgatcct
2281 ggggccagcc caggcgggtg tgtggtttgt gcgagtcccc ttacaccccc acttcctcca
2341 ggggcctcgt ccccatctag tttctctagc gacttcctgg tcccaaacgg ggaaaagctg
2401 ttctatttaa tcgtgtgaag tgtctgtctc ccagccttgg ggcccccgga gcctcccttc
2461 tccaaattgc tgtgaactta cccacatctt gcccttctgt tgtaaatacc cctcacggag
2521 gaaatagttt tgctaagaaa taaaagtgac tatttt
//