GenBank-Updates@genbank.bio.net (04/09/91)
LOCUS TRNSATG 709 bp ds-DNA BCT 09-APR-1991
DEFINITION Transposon Tn7 streptothricin acetyltransferase gene, complete cds,
dhfrI gene, 3' end, aadA1 gene, complete cds.
ACCESSION M63169
KEYWORDS antibiotic resistance; streptothricin acetyltransferase.
SOURCE Transposon Tn7, DNA.
ORGANISM Transposon Tn7
Prokaryota; Bacteria.
REFERENCE 1 (sites)
AUTHORS Sundstroem,L., Roy,P.H. and Skold,O.
TITLE Site-specific insertion of three structural gene cassettes in
transposon Tn7
JOURNAL J. Bacteriol. (1991) In press
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS <1..12
/note="trimethoprim resistance"
/product="type I DHFR"
/gene="dhfrI"
/codon_start=1
CDS 107..631
/product="streptothricin acetyltransferase"
/gene="sat"
/codon_start=107
CDS 689..
/product="aminoglycoside nucleotidyltransferase"
/gene="aadA1"
/codon_start=689
/partial
BASE COUNT 192 a 175 c 180 g 162 t
ORIGIN
1 caaaagggtt aacaagtggc agcaacggat tcgcaaacct gtcacgcctt ttgtgccaaa
61 agccgcgcca ggtttgcgat ccgctgtgcc aggcgttagg cgtcatatga agatttcggt
121 gatccctgag caggtggcgg aaacattgga tgctgagaac catttcattg ttcgtgaagt
181 gttcgatgtg cacctatccg accaaggctt tgaactatct accagaagtg tgagccccta
241 ccggaaggat tacatctcgg atgatgactc tgatgaagac tctgcttgct atggcgcatt
301 catcgaccaa gagcttgtcg ggaagattga actcaactca acatggaacg atctagcctc
361 tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa ggagtcgcgc acagtctcat
421 cgaatttgcg aaaaagtggg cactaagcag acagctcctt ggcatacgat tagagacaca
481 aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt ggctttactc tcggcggcat
541 tgacctgttc acgtataaaa ctagacctca agtctcgaac gaaacagcga tgtactggta
601 ctggttctcg ggagcacagg atgacgccta acaattcatt caagccgaca ccgcttcgcg
661 gcgcggctta attcaggagt taaacatcat gagggaagcg gtgatcgcc
//GenBank-Updates@genbank.bio.net (04/10/91)
LOCUS TRNSATG 709 bp ds-DNA BCT 10-APR-1991
DEFINITION Transposon Tn7 streptothricin acetyltransferase gene, complete cds,
dhfrI gene, 3' end, aadA1 gene, complete cds.
ACCESSION M63169
KEYWORDS antibiotic resistance; streptothricin acetyltransferase.
SOURCE Transposon Tn7, DNA.
ORGANISM Transposon Tn7
Prokaryota; Bacteria.
REFERENCE 1 (sites)
AUTHORS Sundstroem,L., Roy,P.H. and Skold,O.
TITLE Site-specific insertion of three structural gene cassettes in
transposon Tn7
JOURNAL J. Bacteriol. (1991) In press
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS <1..12
/note="trimethoprim resistance"
/product="type I DHFR"
/gene="dhfrI"
/codon_start=1
CDS 107..631
/product="streptothricin acetyltransferase"
/gene="sat"
/codon_start=107
CDS 689..>709
/product="aminoglycoside nucleotidyltransferase"
/gene="aadA1"
/codon_start=689
BASE COUNT 192 a 175 c 180 g 162 t
ORIGIN
1 caaaagggtt aacaagtggc agcaacggat tcgcaaacct gtcacgcctt ttgtgccaaa
61 agccgcgcca ggtttgcgat ccgctgtgcc aggcgttagg cgtcatatga agatttcggt
121 gatccctgag caggtggcgg aaacattgga tgctgagaac catttcattg ttcgtgaagt
181 gttcgatgtg cacctatccg accaaggctt tgaactatct accagaagtg tgagccccta
241 ccggaaggat tacatctcgg atgatgactc tgatgaagac tctgcttgct atggcgcatt
301 catcgaccaa gagcttgtcg ggaagattga actcaactca acatggaacg atctagcctc
361 tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa ggagtcgcgc acagtctcat
421 cgaatttgcg aaaaagtggg cactaagcag acagctcctt ggcatacgat tagagacaca
481 aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt ggctttactc tcggcggcat
541 tgacctgttc acgtataaaa ctagacctca agtctcgaac gaaacagcga tgtactggta
601 ctggttctcg ggagcacagg atgacgccta acaattcatt caagccgaca ccgcttcgcg
661 gcgcggctta attcaggagt taaacatcat gagggaagcg gtgatcgcc
//GenBank-Updates@genbank.bio.net (05/11/91)
LOCUS TRNSATG 709 bp ds-DNA BCT 11-MAY-1991
DEFINITION Transposon Tn7 streptothricin acetyltransferase gene, complete cds,
dhfrI gene, 3' end, aadA1 gene, complete cds.
ACCESSION M63169
KEYWORDS antibiotic resistance; streptothricin acetyltransferase.
SOURCE Transposon Tn7, DNA.
ORGANISM Transposon Tn7
Prokaryota; Bacteria.
REFERENCE 1 (sites)
AUTHORS Sundstroem,L., Roy,P.H. and Skoeld,O.
TITLE Site-specific insertion of three structural gene cassettes in
transposon Tn7
JOURNAL J. Bacteriol. 173, 3025-3028 (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS <1..12
/note="trimethoprim resistance"
/product="type I DHFR"
/gene="dhfrI"
/codon_start=1
CDS 107..631
/product="streptothricin acetyltransferase"
/gene="sat"
/codon_start=107
CDS 689..>709
/product="aminoglycoside nucleotidyltransferase"
/gene="aadA1"
/codon_start=689
BASE COUNT 192 a 175 c 180 g 162 t
ORIGIN
1 caaaagggtt aacaagtggc agcaacggat tcgcaaacct gtcacgcctt ttgtgccaaa
61 agccgcgcca ggtttgcgat ccgctgtgcc aggcgttagg cgtcatatga agatttcggt
121 gatccctgag caggtggcgg aaacattgga tgctgagaac catttcattg ttcgtgaagt
181 gttcgatgtg cacctatccg accaaggctt tgaactatct accagaagtg tgagccccta
241 ccggaaggat tacatctcgg atgatgactc tgatgaagac tctgcttgct atggcgcatt
301 catcgaccaa gagcttgtcg ggaagattga actcaactca acatggaacg atctagcctc
361 tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa ggagtcgcgc acagtctcat
421 cgaatttgcg aaaaagtggg cactaagcag acagctcctt ggcatacgat tagagacaca
481 aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt ggctttactc tcggcggcat
541 tgacctgttc acgtataaaa ctagacctca agtctcgaac gaaacagcga tgtactggta
601 ctggttctcg ggagcacagg atgacgccta acaattcatt caagccgaca ccgcttcgcg
661 gcgcggctta attcaggagt taaacatcat gagggaagcg gtgatcgcc
//