GenBank-Updates@genbank.bio.net (05/11/91)
LOCUS VIBNEUP 2070 bp ds-DNA BCT 11-MAY-1991
DEFINITION V.proteolyticus neutral protease gene, complete cds.
ACCESSION M64809
KEYWORDS neutral protease.
SOURCE V.proteolyticus (ATCC 15338) DNA, clone pVP8.
ORGANISM Vibrio proteolyticus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 2070)
AUTHORS David,V.A., Deutch,A.H., Ally,A., Pawlyk,D., Sloma,A. and
Durham,D.R.
TITLE Cloning, sequencing and expression of the gene encoding the
extracellular neutral protease (vibriolysin) of Vibrio
proteolyticus
JOURNAL Unpublished (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
-10_signal 21..27
misc_feature 151..222
/note="leader sequence"
CDS 151..1980
/product="neutral protease"
/codon_start=151
mat_peptide 742..1977
/product="neutral protease"
/codon_start=742
BASE COUNT 565 a 469 c 516 g 520 t
ORIGIN
1 aattgacgtg ggatataaaa tatttttctt acaaactgga atgttacaga aatgtaatta
61 atggttattt cgcgagttat cgcaggaggg tttaatttct gatttatcag tagttaaaca
121 acgattgaaa ataatctcca ggattgagaa atgaataaaa cacaacgtca catcaactgg
181 ctgctggctg ttagcgcggc aactgcgcta cctgtcaccg ctgcagaaat gatcaacgta
241 aatgatggca gcctgctaaa ccaggctctt aaagctcagt cacagagcgt tgccccggtg
301 gaaaccggat tcaaacaaat gaaacgagtt gttttgccaa atggcaaagt gaaagttcgt
361 tatcaacaaa ctcaccacgg tctaccggtt ttcaacacct cggtagtggc gactgaatcg
421 aagtctggta gtagcgaagt gttcggtgtg atggctcagg gtatcgcaga cgacgtgtct
481 acactgacgc catccgttga gatgaagcag gccatttcaa ttgctaaatc gcgtttccaa
541 cagcaagaaa aaatggttgc ggaacctgca acggaaaacg aaaaagccga gttgatggtt
601 cgtctggacg acaacaatca agcgcaacta gtgtatctgg ttgatttctt cgttgccgag
661 gatcacccag cgcgtccttt ctttttcatt gatgcgcaaa cgggtgaagt actgcaaact
721 tgggatggtc tgaaccatgc acaagctgac ggtactggcc ctggcggtaa caccaaaaca
781 ggtcgttatg aatacggttc tgactttcct ccgtttgtca tcgataaagt cggcactaag
841 tgttcaatga acaacagcgc ggtaagaacg gttgacctga acggctcaac ttcaggtaac
901 accacttaca gctatacctg taacgactca accaactaca acgattacaa agccattaac
961 ggcgcgtact cgccactgaa cgatgcccac tacttcggta aagtggtttt cgatatgtac
1021 aaagactgga tgaacaccac accactgacg ttccagctga ctatgcgtgt tcactatggt
1081 aacaactacg aaaacgcgtt ctggaatggt tcatccatga ccttcggtga tggctacagc
1141 accttctacc cgctggtgga tattaacgtt agtgcccacg aagtgagcca cggtttcacc
1201 gaacaaaact cgggtctggt gtacgagaat atgtctggtg gtatgaacga agcgttctct
1261 gatattgcag gtgaagcagc agagttctac atgaaaggca gcgttgactg ggttgtcggt
1321 gcggatatct tcaaatcatc cggcggtctg cgttactttg atcagccttc gcgtgacggc
1381 cgttctatcg accatgcgtc tgactactac aatggcctga atgttcacta ctcaagtggt
1441 gtattcaacc gtgcgttcta cctgctggct aacaaagcgg gttgggatgt acgcaaaggc
1501 tttgaagtgt ttaccctggc taaccaattg tactggacag cgaacagcac atttgatgaa
1561 ggcggttgtg gtgtagtgaa agctgcgagc gacatgggtt acagcgttgc agacgtagaa
1621 gatgcgttta acacggtagg cgttaacgcg tcttgtggtg caactcctcc tccgtctggc
1681 gatgtactgg aaatcggtaa accgctggcg aacctttcag gtaaccgcaa tgacatgact
1741 tactacacgt tcacaccaag cagctcatct agcgtagtga ttaagatcac tggcggtaca
1801 ggtgatgcag acctttacgt gaaagcgggt agcaagccaa ccacgacttc ttacgattgc
1861 cgtccatata agtatggtaa cgaagagcag tgttcaattt cagcgcaagc gggtactacg
1921 tatcacgtta tgctgcgtgg ttacagcaat tacgctggtg taactttgcg tgctgactaa
1981 actcagaatg gaaccagtga aggcgcacct taaggtcgcc ttttttgtat caggcgatct
2041 gtgtaaacgt gacctgatcg aagtgaggat
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