GenBank-Updates@genbank.bio.net (05/14/91)
LOCUS RATPKRL1 2717 bp ds-DNA ROD 14-MAY-1991
DEFINITION Rat pyruvate kinase gene, exons 1 and 2.
ACCESSION M17088 J03455
KEYWORDS pyruvate kinase.
SEGMENT 1 of 4
SOURCE Rat DNA (library of J.Bonner), clone lambda-LPK30, and
reticulocyte, cDNA to mRNA, clones pRPK[1,3].
ORGANISM Rattus norvegicus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 2717; 1 to 1490; 1978 to 2222)
AUTHORS Noguchi,T., Yamada,K., Inoue,H., Matsuda,T. and Tanaka,T.
TITLE The L- and R-type isozymes of rat pyruvate kinase are produced from
a single gene by use of different promoters
JOURNAL J. Biol. Chem. 262, 14366-14371 (1987)
STANDARD full staff_entry
COMMENT Draft entry and printed copy of sequence for [J. Biol. Chem. 262,
14366-14371 (1987)] kindly provided by
T.Noguchi, 09-SEP-1987.
FEATURES Location/Qualifiers
prim_transcript 925..>2717
/note="PK mRNA and introns (alt.)"
prim_transcript 930..>2717
/note="PK mRNA and introns (alt.)"
prim_transcript 934..>2717
/note="PK mRNA and introns (alt.)"
exon 980..1079
/note="R-pyruvate kinase, exon 1"
intron 1080..2007
/note="PK intron A"
exon 1446..1452
/note="L-pyruvate kinase, exon 1"
intron 1453..2007
/note="PK intron A (alt.)"
exon 2008..2190
/note="R-pyruvate kinase, exon 2"
exon 2008..2190
/note="L-pyruvate kinase, exon 2"
intron 2191..>2717
/note="PK intron B"
BASE COUNT 666 a 637 c 739 g 675 t
ORIGIN 342 bp upstream of PstI site.
1 agtacccaga tccatccctg acagcccctg ccctgtttat agcatttatt tatttgctag
61 tagtctgtcc cccacccctg ctatactata atccacagac gggaaagttt ccatctgcct
121 gactcccact gtatcctcaa ggtctgtcac tggccggtgt catagtgaga atgccacgga
181 aggcctccca gagaaccccc ctgttttatc aactttgctg aacatgcaaa accacctaag
241 actggagtca ggaggatccc cagagtaggg ccttctggga ttcaagagag cagtaagtgc
301 caggtggtcc ccttggggta gtgatagggg attctgcctg caggactgca gtttaccatc
361 atggtggtca acaaggcaag gtcattctcc ctggtgctat ggtatgatct ccctggtgag
421 atcgatgccc ttgtgatggc tgaggagatc ccctgtactc aggtttccat gtgtttatcc
481 atacggctgg tcaacaacaa tacaagttta tgagttagtg cctaggaatg ttcatggttt
541 catctctggg ttctcagagg aacatgtgcg agaaactgag agaccctcta acctttaact
601 accaaagggt atccctgttt tccacagcct agtccccacc ttcaagctct tgttctgaga
661 atacacctaa gattctcttc aataaaatgc aaagagaaag gcattgtact gttggcaatc
721 aaccaatctc ttctctaatg atgagccaac aatgtgaatg gactaatgat tgatcaggaa
781 aaaatagatg acctagggga aggggagaga tgggggcccc acaacaggca gtccttgaga
841 agggactctg tggggatggg gctgcctgct gggtgtgccc cttttctatt ctctggcttc
901 ctcagataag accagcagtt agggtacact ctcctcccag tctgccgttc ttgcagacac
961 agttccacgc tttggaagca tgtctgtcca ggagaacact ctaccccagc agctctggcc
1021 gtggatcttt aggtcccaaa aagacttggc aaagtctgcc ttaagtgggg ctcccggagg
1081 taagaagagg aagggaagcc actgaagaga gaggagaatt agggtagaat cagcgttgag
1141 agatggaggc cttgtggggt aggatgccca atatagcctc acctcggcta aatacagacc
1201 tgatctgagc ctttgatcca ggctctgcag acaggccaaa ggggatccag cagcatgggc
1261 gccacggggc actcccgtgg ttcctggact ctggccccca gtgtacaagg cttccgttgg
1321 caagagagat gctagctggt tatactttaa ccaggactca tctcatctga gccaggcccc
1381 atcccactga caaaggcgca gtataaagca gacccacaga cacagcaggt aagcaacgta
1441 gcagcatgga aggtgcttct aaaccgggtg ctctactgag cagggctggg tcaagctacg
1501 gatccgccag ggctgggtca agctacggat ccgccggatg gatcagcgca gccttgaatg
1561 ggtggaggga aggtggaggg gaggggagtg gagaagccag agaattgcct ggagctggaa
1621 gaagtaaaca agaaaatgag ggaaagtaac tgagtgtttg caggcctctc tgggagggct
1681 gaagctgagt ggtctgcagc ggtgtgacgt tggggaaggt ctctgtagct tggggataga
1741 cctggatttg aatcatctca cttggctgat gcattttttt tttcttagac aatgtatttt
1801 tttttccccc ttggtctttt tcttacttgt aaagtgggga tagagcagga ccctggagac
1861 taaacaaagt agcgggtgtg tgatagtcaa atgggaggaa gacctcgcac catctggaga
1921 tctgaataga cacagggtgc actggcagga agcacatggg ggacaggcag gtgtctgtgc
1981 aagagcctga gtttccatct acggcagggc cagcgggata ccttcgacgt gcgagtgtgg
2041 ctcaactgac ccaggagctg ggcactgcct tcttccagca gcagcaactg cccgcagcta
2101 tggcggacac cttcctggaa cacctctgcc ttctggatat cgactcacag cctgtggctg
2161 ctcgtagcac cagcatcatt gccaccattg gtaagaccac ccaagcccta gaagccacac
2221 agggccaccc acatggctca ggtgtctcct tactttgcta tccttgttgt ggtcattcat
2281 tcaacaaata tttgtgagat ttgtgcaggg gtagggggtg ggtttgtttt tgtttttgtt
2341 tttgtttttt tgagatcggg tctttttatg ttgtagctct gactgatgga taggcaggtc
2401 tcaaattaga aatctcctac ctttgcctcc tgagagttgg actgggacca ccttgctcca
2461 catgaggctg ggtttttgag acagggtctc aggtagcctt ggatatttct agcttatttc
2521 atagacccta ttatatcgtc ttagcctggt gaaagcatag gtatgtgata ccacacccaa
2581 cttaacaaat gcttgttgag tacctggaaa acaaggtttg gaattggggg gtagactcta
2641 tctcaaaaac aaataagaac gaggaaaggt gtcatctgca cacagatggc cttccaagga
2701 taaggacggc tgggatc
//