GenBank-Updates@genbank.bio.net (05/14/91)
LOCUS RATPKRL2 3158 bp ds-DNA ROD 14-MAY-1991
DEFINITION Rat pyruvate kinase gene, exons 3-9.
ACCESSION M17089 J03455
KEYWORDS pyruvate kinase.
SEGMENT 2 of 4
SOURCE Rat DNA (library of J.Bonner), clone lambda-LPK30, and
reticulocyte, cDNA to mRNA, clones pRPK[1,3].
ORGANISM Rattus norvegicus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 3158; 584 to 1012; 1088 to 1318; 1852 to 3075)
AUTHORS Noguchi,T., Yamada,K., Inoue,H., Matsuda,T. and Tanaka,T.
TITLE The L- and R-type isozymes of rat pyruvate kinase are produced from
a single gene by use of different promoters
JOURNAL J. Biol. Chem. 262, 14366-14371 (1987)
STANDARD full staff_entry
COMMENT Draft entry and printed copy of sequence for [J. Biol. Chem. 262,
14366-14371 (1987)] kindly provided by
T.Noguchi, 09-SEP-1987.
FEATURES Location/Qualifiers
prim_transcript <1..>3158
/note="PK mRNA and introns (alt.)"
intron <1..609
/note="PK intron B (no splice consensus)"
exon 609..699
/note="R-pyruvate kinase, exon 3"
exon 609..699
/note="L-pyruvate kinase, exon 3"
intron 700..843
/note="PK intron C (no splice consensus)"
exon 844..975
/note="R-pyruvate kinase, exon 4"
exon 844..975
/note="L-pyruvate kinase, exon 4"
intron 976..1110
/note="PK intron D (no splice consensus)"
exon 1111..1297
/note="R-pyruvate kinase, exon 5"
exon 1111..1297
/note="L-pyruvate kinase, exon 5"
intron 1298..1867
/note="PK intron E (no splice consensus)"
exon 1868..2138
/note="R-pyruvate kinase, exon 6"
exon 1868..2138
/note="L-pyruvate kinase, exon 6"
intron 2139..2244
/note="PK intron F (no splice consensus)"
exon 2245..2395
/note="R-pyruvate kinase, exon 7"
exon 2245..2395
/note="L-pyruvate kinase, exon 7"
intron 2396..2600
/note="PK intron G (no splice consensus)"
exon 2601..2753
/note="R-pyruvate kinase, exon 8"
exon 2601..2753
/note="L-pyruvate kinase, exon 8"
intron 2754..2846
/note="PK intron H (no splice consensus)"
exon 2847..3014
/note="R-pyruvate kinase, exon 9"
exon 2847..3014
/note="L-pyruvate kinase, exon 9"
intron 3014..>3158
/note="PK intron I (no splice consensus)"
BASE COUNT 716 a 806 c 890 g 746 t
ORIGIN About 1.86 kb after segment 1.
1 ttaagcccaa ggttataccc agaagcaaga gcaaacccaa ggctagcctg gttatggagt
61 ccttgtctca gaaggaaggc tgttattcct aaagaatggg agagaaggat ggtgtagctc
121 agtactatag catttatttg cctcgcatgt agaaggccct ggcctccatc tccagagcag
181 aaaaaaaaaa tggtggccag ggaagaagag ggagggggaa gtgcatacag cgagctgctc
241 gtgctgctgc aggctgacgt ggatgaagac agggacagat ctcagttctg tcagtgagat
301 tgctcagctt tcaaagtgcc tttttgacct gcctggcgtt ctgacccttc acagcctaca
361 gtaacctcca cagcatacac ttatgtcagg tcacttgcta acaaaggtgc ctgaggcctg
421 actgagattt agacattcca caagctgcct tcccaagggc tgtgtactgg ttggagtggg
481 ggaaaaaaag agtttgtgag tagaggaact tcgaaggact cctgggtagg ggagtctctc
541 tcgattgttg cagatgggtt tgagtgtcag gcttgggagg accttcttag tgggttctct
601 ggttccaggg ccagcatccc gctctgtgga ccgcctcaag gagatgatca aagcagggat
661 gaacattgca cgactcaact tctcccatgg ctcccatgag gtgtggggaa gaagtcatgg
721 agggtggagc tggagaatgc cccaaggtcc tgttcacttt gccttaagat ccaggttttg
781 ttctctctct ctctctctct ctctctctct ctctctctct ctctctcgct ttctctctgg
841 gtagtaccat gcagaataca tcgccaacat ccgggaggca actgagagtt ttgcaacctc
901 cccactcagc tacagacctg tggccatcgc cctggacacc aagggacctg agatacgaac
961 cggagtcttg cagggggtga gaagcctttg ggtccgagct gtctggggcc tggaggagca
1021 tgagagccct gggagcaaga gagagggtcc ctaccctgtg agggtccact tgcaaaactc
1081 aggcttctct acccacccca ctcccctcca gggtccggag tcggaggtgg aaattgtgaa
1141 gggctcacag gtgctggtga cggtggaccc gaagttccag acaaggggtg atgcaaagac
1201 agtgtgggtg gactaccaca atatcacccg ggtcgttgca gtggggggcc gcatctacat
1261 tgacgacggg ctcatctcct tagtggtaca gaaaatcggt acagaaatgc atcctgatct
1321 tatgaggcgc tcccaaaacc ctgaaacagg gcattttctc ttccctttgg tttctatgtt
1381 ctgtcagctc cttacccata ccttccccta gtggtgcttg cttctccacc tgtcctagtg
1441 gtccctgctg tcatcatggg ttggagctga gcatttgatg cctcttcttg tgtgtggagt
1501 ggagtgaagg gttcagaagt tccaatttct ggaggatcca agcttaagag ttccctctgc
1561 agcctgcgaa tcctaatcac atacccattt gtcataggta ccagtgccta ccccgtcaag
1621 gcattgtgct aagtacaatg tgtagccact gccaagtaga agtgactcaa agccctttaa
1681 caagggtgtc cccaggccac agtcctgcca ggtgcaggct tgggcccaca gccctgcctt
1741 tattaagcac aagtacttgc tgcttccttc actgccgagt gactagatgg ctgtctccct
1801 gtcagtgacc ctgcagtgcc tatatcgcag ctcagcctta cagacactgg gtctctctat
1861 acccgcaggc ccagagggac tggtgacaga agtggagcac ggtggtatct tgggcagcag
1921 gaagggtgtg aacttgccaa acactgaggt ggacctgccc gggctgtctg agcaagacct
1981 tttggatctg cgcttcgggg tgcagcataa tgtggacatc atctttgcct cctttgtgcg
2041 gaaagccagt gacgtgttag cagtccggga tgccctgggg ccagaaggac agaacatcaa
2101 aattatcagc aaaatcgaga accatgaagg cgtgaagaag tgagcttagt ctttggtccg
2161 tcagccctct gtttctcttt ccctctcttc tgcatgatat ttcctgccat ctcctcctct
2221 gcctacctga tcctccaacc accaggtttg atgaaattct agaagtgagc gatggcatca
2281 tggtggcacg gggtgacctg ggcattgaga tccctgcgga gaaggttttc ttggctcaga
2341 agatgatgat tggacgctgc aacctggccg gcaagcctgt cgtttgtgcc acacaggtct
2401 ggagcaaacc cttgggcttt aaaggtgctg agaaacctac atgttgctgt ctggtgcctg
2461 gtcacaaaag acgatgacat gaacttccag ggtcttactg tgaccctggc actctaagcg
2521 tgcaggtgcc agagaaatac tgggcggttc ggtccccatc agaatgtgac tctacagctt
2581 tgacatccat gtgtcactca gatgctggag agcatgatca ctaaggctcg accaactcgg
2641 gcggagacaa gcgatgtggc caatgccgtg ctggatgggg ctgactgtat catgctgtcc
2701 ggagagaccg ccaagggcag ttttcctgtg gaagctgtaa tgatgcaaca tgcggtaggt
2761 actccgttct gaaaagctgc tgggccagcg acacagaccc ctctggaacc ccgttgacta
2821 ctcaacctga cctactggtc cctgcagatt gcgcgggagg cagaggccgc tgtgtaccac
2881 cgccagttgt ttgaggagct acgccgggca gcgccgctga gccgtgaccc aactgaggtc
2941 actgcgattg gagccgtgga ggcttccttc aagtgctgtg cagcagccat catcgtgctg
3001 acgaagactg gccggtgaga aacatattgg aaacagggct ttgggcaggg gcggctgaca
3061 agaggctaat aaaaaaagca ctgctttagg gcaagcaaag aaacagccta cggtactaga
3121 ggccaggaga ggctagaggc ttcggacagg tggctttt
//