GenBank-Updates@genbank.bio.net (05/14/91)
LOCUS RATPKRL4 1690 bp ds-DNA ROD 14-MAY-1991
DEFINITION Rat pyruvate kinase gene, exon 11.
ACCESSION M17091 J03455
KEYWORDS pyruvate kinase.
SEGMENT 4 of 4
SOURCE Rat DNA (library of J.Bonner), clone lambda-LPK30, and
reticulocyte, cDNA to mRNA, clones pRPK[1,3].
ORGANISM Rattus norvegicus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1690; 197 to 1688)
AUTHORS Noguchi,T., Yamada,K., Inoue,H., Matsuda,T. and Tanaka,T.
TITLE The L- and R-type isozymes of rat pyruvate kinase are produced from
a single gene by use of different promoters
JOURNAL J. Biol. Chem. 262, 14366-14371 (1987)
STANDARD full staff_entry
COMMENT Draft entry and printed copy of sequence for [J. Biol. Chem. 262,
14366-14371 (1987)] kindly provided by
T.Noguchi, 09-SEP-1987.
FEATURES Location/Qualifiers
prim_transcript <1..1613
/note="PK mRNA and intron"
intron <1..225
/note="PK intron J (no splice consensus at 225)"
CDS join(M17088:980..1079,M17088:2008..2190,M17089:609..699,
M17089:844..975,M17089:1111..1297,M17089:1868..2138,
M17089:2245..2395,M17089:2601..2753,M17089:2847..3014,
M17090:457..638,225..331)
/note="R-pyruvate kinase"
/codon_start=980
CDS join(M17088:1446..1452,M17088:2008..2190,M17089:609..699,
M17089:844..975,M17089:1111..1297,M17089:1868..2138,
M17089:2245..2395,M17089:2601..2753,M17089:2847..3014,
M17090:457..638,225..331)
/note="L-pyruvate kinase"
/codon_start=1446
exon 225..331
/note="R-pyruvate kinase, exon 11"
exon 225..331
/note="L-pyruvate kinase, exon 11"
BASE COUNT 433 a 460 c 339 g 458 t
ORIGIN About 0.8 kb after segment 3.
1 agtttcagaa gagcgtaact aaattttaat ttatagttgt ccgtgtccca ttactgcagc
61 cacatgggta aaaggctcaa tgctgaaaaa cagtatacag catccctcca cacacacatc
121 ctcaaaccac tgccttgttt cctttcccct acagatagta ccttaggtgt cgatgctaat
181 ggctgaccca gttcttcaat gactgtcata tttttctcca ccaggaaagc tccgtggttt
241 cctccgtgtg ggtgatctgg tgattgtggt gacaggttgg cggcctggct ctggctatac
301 caacatcatg cgggtgctga gcgtatcctg aaatgcctct ccccattctg acccagttac
361 accctatttc tttcaaccca cacccctccc atagtcctac atctgccatc tagccccatc
421 cctgtgcttt acacaggccc tgaatgtctg tgtccaatta tacagtggcc accggcagca
481 tcggttgtat atccctgtct caatccgctc agctggactc taagataccc tgagccttta
541 atcccagccc agctggttga ttcgattcct tccgggtccc aatcattgga atggggagtg
601 gaaacagggt gatcttgtcc aattttcata caatcatgat tttaaaacac tgtctgatat
661 aaccctcatg atcagtttcc tagcaaagtg tcatctccta atggcctcaa gtcagggcag
721 aatactcctt caagagcaca gctccacact ttagggaagg ctggggcagc tgggtactgg
781 agagaactaa gacaggctgg cttttctctc tctctctctc tctttttttt tttcttttct
841 ttttcttttt ttcggagctg gggaccgaac ccagggcctt gtgcttgcta ggcaagcgct
901 ctaccactga gctaaatccc caaccccagc ttttctcttt ttaatacaag ctctcactgg
961 cctcaaactc ctaagtcctc ctgcctggcc ctcctaaggg tagggactac aggcatgagt
1021 gaccagctgg acttcgggta gccttatttt cttactgact ccacaaacca tggttgttct
1081 cctgcccact gctctgctgg gtcagatgat ccagaaattc ttccacaacc acttggctcc
1141 cacatacaaa ttagaagcaa aactgaatct tttcttttaa acccaactgt ttaggtgcaa
1201 ttataaaaac aactcccaca ggcaaagaat cccagaatct cctaccctag gagatgtata
1261 gtcctggccc cacccatcaa tgctgtagta tactcctgaa gcgggacaga actggtggac
1321 aggggactcc tcttgtccct aagaaagtgg aggcactgtt ggcccacccc tcctaggttt
1381 gaatactcca ggccctcctc ttcagcacca acagcaaatc cagatgagga aaaaaaaata
1441 agtgcagttc tcctgctgcc ctcctctttt cactacctca atacagcaag tttgagtatt
1501 gctgctgatg gcagtgtgca aggaccacaa agatgtcccc cctcagcccc ctagcagaag
1561 gtggagagga cagaggaatg aataataaag tgaatgcgtc aaattagcaa atgaataaat
1621 aaaattaata agtgactgaa taagacacag ggaaagatta aaaggtcaca tgaggattca
1681 gtcccctttt
//