GenBank-Updates@genbank.bio.net (05/14/91)
LOCUS HEITRANS 9100 bp ds-DNA VRL 14-MAY-1991
DEFINITION Haemophilus influenzae transformtion gene cluster DNA.
ACCESSION M62809
KEYWORDS transformation.
SOURCE Haemophilus influenzae Rd DNA.
ORGANISM Haemophilus influenzae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Pasteurellaceae.
REFERENCE 1 (bases 1 to 9100)
AUTHORS Tomb,J.-F., El-Hajj,H. and Smith,H.O.
TITLE Nucleotide sequence of a cluster of genes involved in the
transformation of Haemophilus influenzae Rd
JOURNAL Gene (1991) In press
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS complement(135..980)
/number=3
/note="The predicted molecular weight and pI of ORF J are
32.2 Kd and 8.96 respectively"
/note="ORF J"
/codon_start=980
/citation=Gene ??, ??-?? (1991)
CDS complement(1068..3662)
/number=3
/function="Possibly involved in the elongation of the
murein sacculus."
/note="An insertion in ORF I causes a modest deficiency in
transformation. ORF I is 51.8 % identical to the
penicillin-binding protein 1A of E.coli. The predicted MW
and pI of ORF I are 95.4 KD and 7.1"
/note="ORF I"
/codon_start=3662
/citation=Gene ??, ??-?? (1991)
CDS 3761..4558
/number=1
/note="A Haemophilus strain carrying a mini-Tn10kan
insertion in ORF A is deficient in transformation. The
predicted molecular weight and pI of ORF A are 31.5 Kd and
6.0 respectively"
/note="ORF A"
/codon_start=3761
/citation=Gene ??, ??-?? (1991)
CDS 4559..5065
/number=2
/note="The predicted molecular weight and pI of ORF B are
19.7 Kd and 9.0 respectively"
/note="ORF B"
/codon_start=4559
/citation=Gene ??, ??-?? (1991)
CDS 5062..5583
/number=3
/note="A Haemophilus strain carrying a mini-Tn10kan
insertion in ORF C causes a deficiency in transformation.
The predicted molecular weight and pI of ORF C are 19.9 Kd
and 10.2 respectively"
/note="ORF C"
/codon_start=5062
/citation=Gene ??, ??-?? (1991)
CDS 5580..5993
/number=4
/note="The predicted molecular weight and pI of ORF D are
15.6Kd and 4.6 respectively"
/note="ORF D"
/codon_start=5580
/citation=Gene ??, ??-?? (1991)
CDS 6003..7328
/number=5
/note="A mini-Tn10kan insertion in ORF E causes a
deficiency in transformation. ORF E is 24% identical to
the products of the filamentous phage gene IV and of pulD,
a gene in the pulC operon of Klebsiella"
/note="ORF"
/codon_start=6003
/citation=Gene ??, ??-?? (1991)
CDS complement(6494..6883)
/number=1
/note="A Haemophilus strain carrying an insertion in ORF H
is deficient in transformation. The predicted molecular
weight and pI of ORF H are 15.6 Kd and 12.2 respectively"
/note="ORF H"
/codon_start=6883
/citation=Gene ??, ??-?? (1991)
CDS 7350..8039
/number=6
/note="A Haemophilus strain carrying an insertion in ORF F
is deficient in transformation, but is still able to bind
and take up DNA. The predicted molecular weight and pI of
ORF F are 26.8 Kd and 10.6"
/note="ORF F"
/codon_start=7350
/citation=Gene ??, ??-?? (1991)
CDS 8115..8711
/number=7
/note="The predicted molecular weight and pI of ORF G are
21.8 Kd and 4.3 respectively"
/note="ORF G"
/codon_start=8115
/citation=Gene ??, ??-?? (1991)
BASE COUNT 2751 a 1669 c 1823 g 2857 t
ORIGIN
1 gtcaaaaatt gcgtgcattc tagcgaaaaa atgggctttt gggaactgtg ggatttattt
61 aaaatcttag aaaatcttac cgcactttta agctataaag tgcggtgaaa tttagtggcg
121 tttataatgg agaattactc tggtgtaatc cattcgactg tccagcttcc agtaccttct
181 ggaactaatg tttttgtgag ataaggcaaa atttctttca tttgggtttc taatgtccaa
241 ggtggattaa ttaccaccat accgctcgca gtcattcctc gttgatcgct atctgggcga
301 acggcgagtt caatttttag aatttttcta attcccgttg cttctaaacc cttaaaaata
361 cgtttagttt gttggcgtaa tacaacagga taccaaatcg cataagtgcc agtggcaaaa
421 cgtttatagc cctcttcaat ggctttaaca acgagatcat aatcatcttt taattcataa
481 ggcggatcga tgagtactaa gcctcggcgt tcttttggcg gaagcgttgc tttgacttgt
541 tgaaagccat tgtcacattt tacggtgaca tttttgtcgt cgctaaaatt attgcgaaga
601 attggataat cgctaggatg aagctcggtc aatagtgcgc gatcttgtga gcgcaacaat
661 tccgcggcaa ttaatggaga acccgcgtaa taacgtagtt ctttgccacc ataattgagt
721 tttttgatca tttttacata acgagcaata tcttcgggta aatctgtttg atcccacagg
781 cgtccaatac cttctttata ttcccccgtt ttttctgatt catttgagga taaacgataa
841 cgccccacac cagagtgcgt atccaaataa aaaaagcctt tttctttgag tttaagattt
901 tccaaaatga gcattaaaac aatatgtttc aagacatcgg catgattgcc agcgtgaaat
961 gagtgatgat aactcagcat aatatattcc ttatatattc cttatttgtt taataacgaa
1021 ggcgagccaa ttgactcgcc cgattacaca ctaaagtgcg gtcattttta gaagagttct
1081 tgtggttgcg tcgctggcgt attgccttca ttatttaagc gttgctgtaa ctcagtagga
1141 acataataac cacgctcttg catttccgaa agataggtac gtgtcggttc tgttcccgca
1201 ataaaatatt ctttgcgccc accgtttgga gaaagcaaac ctgtcaaagt atcaatgttt
1261 ttttccacaa tttttggcgg tagcgacaat ttacgttctg gcttatcact caaagccgtt
1321 ttcatataag tgatccaagc aggcattgct gtttttgctc ctgcttctcc acgcccaagt
1381 actcgtttgt tatcatcaaa cccgacataa gttgtggtta ctaagtttgc accaaatccc
1441 gcataccaag ccacttttga actgttggta gtacctgttt taccgcctat atcgctacgt
1501 ttaatgcttt gtgcaatacg ccagctggtg cctttccagt ctaaaccttg ttcgccataa
1561 attgccgtat ttaaggcact acgaatgaga aaagcaagtt cgccactaat gacacgtggc
1621 gcatattcta ttttcgacga agcatttttt gcagcagcca ttaaatcaat cgcatcttct
1681 ttaagtgcgg tcatatttga ttgtaattct ggcagttcag gcacagtttc aggttgttga
1741 tctaattctt cgccattggt gctgtcatct gttggtttta aggcattctc gcctaaagga
1801 atattggcaa agccgttgat tttgtctttg gtttcgccat aaattacagg tatatcatta
1861 cattcaatgc aagcaatttt agggtttgca ataaataagt ctttacccgt gttatcttga
1921 attttttcaa tgatataagg ttcaatgagg aagccaccat tatcaaacac cgcataagct
1981 cgcgccattt ctaatggtgt gaaagaggct gcgccaagtg ctaaggcttc actggcaaaa
2041 tattgatcac gtttaaaacc aaaacgttgt aaaaattctg ctgtgaaatc aatacctgcc
2101 gtttggatag cacgaatagc aattatattt ttggattgac ctaatcctac gcgtaaacgc
2161 atcgggccat cataacgatc aggcgagttt ttcggttgcc acattttttg tcccggtttt
2221 tgaatagaaa tcgggctgtc ttgtaatacg cttgaaagtg ttaagccttt ttctaatgct
2281 gccgcgtaaa taaatggttt gatagaagaa cccacttgaa ctaaagactg tgtggctcga
2341 ttgaatttac tttgttcata gctaaagcca ccgaccactg cttcaatcgc accattatct
2401 gaattaagag aaactaatgc tgaatttgct gcgggaattt gtcctaattg ccattcccca
2461 ttagcacgct gatgaatcca aatttgctcg ccgactttca caggattgct tctgcctgtc
2521 caacgcattg cattggttga taaggtcatt ttttccccag aagcgagcaa tatatcagca
2581 ccgcctttta caattccaat cactgccgca ggaataaatg gctctgaatc aggtagtttg
2641 cgtagaaaac cgacaatgcg atcattgtcc caagcggctt catttttttg ccataatggc
2701 gcgccaccgc gataaccgtg acgcatatcg taatcaatca agttattacg cacagctttt
2761 tgggcttcag cttggtcttt tgaaagtaca gtggtaaata ctttataacc actggtgtaa
2821 gcattttctt cgccaaaacg acgcaccatt tcttgacgca ccatttcagt gacataatcg
2881 gctcgaaatt caaattttgc gccgtgatag ctcgccacaa tcggctcttt caatgcagca
2941 tcatattctt ctttgctgat gtatttttca tctaacatac ggcttagcac cacattgcgg
3001 cgttcttctg aacgttttaa agaataaagc gggttcattg ttgaaggtgc tttaggtaaa
3061 ccagcaataa tcgccatttc cgataaggtc aattcattca atgatttacc gaaataggtt
3121 tgtgctgccg ctgcaacacc ataagaacga tagcctaaaa agattttgtt taaataaagc
3181 tctaatattt cttgtttgtt gagagtattt tcgatttcta ccgcaagcac ggcttcacga
3241 gctttacgaa taatggtttt ttctgaggtt aagaaaaagt tacgcgctaa ttgttgagta
3301 atcgtacttg cgccttgtga tgcaccgcca ttactcactg cgacaaacaa tgcacgggca
3361 atgccgatag ggtctaatcc gtgatgatcg taaaaacgac tgtcttccgt cgctaaaaat
3421 gcgtcaatta agcgttgtgg cacatcggct aatttcactg gaatacggcg ttgctcaccc
3481 acttcgccaa ttaatttacc gtcagccgta taaatctgca ttggttgctg taattcaacg
3541 gtttttaatg tttctactga gggcaattca gattttaagt ggaaatacaa cattccgcct
3601 gctactaaac ctaaaataca taaagttaat agggtgttta atattaattt tgcgatccgc
3661 atcgtaaaat tctcgcttcg ttaatgaata ttcttgtcaa gagacctatg atttggctgt
3721 taagtataaa agattcagcc tttaaagaat aggaaagaat atgcaattct ccctgaaaaa
3781 ttaccgcact ttacaaatcg gcattcatcg taagcagagt tattttgatt ttgtgtggtt
3841 tgatgatctc gaacagccac aaagttatca aatctttgtt aatgatcgtt attttaaaaa
3901 tcgtttttta caacagctaa aaacacaata tcaagggaaa acctttcctt tgcagtttgt
3961 agcaagcatt cccgcccact taacttggtc gaaagtatta atgttgccac aagtgttaaa
4021 tgcgcaagaa tgtcatcaac aatgtaaatt tgtgattgaa aaagagctgc ctattttttt
4081 agaagaattg tggtttgatt atcgttctac cccgttaaag caaggttttc gattagaggt
4141 tactgcaatt cgtaaaagta gcgctcaaac ttatttgcaa gattttcagc catttaatat
4201 taatatattg gatgttgcgt caaatgctgt tttgcgtgca tttcaatatc tgttgaatga
4261 acaagtgcgg tcagaaaata ccttattttt atttcaagaa gatgactatt gcttggcgat
4321 ttgtgaaaga tctcagcaat cacaaatttt acaatctcac gaaaatttga ccgcacttta
4381 tgaacaattt accgaacgtt ttgaaggaca acttgaacaa gtttttgttt atcaaattcc
4441 ctcaagtcat acaccattac ccgaaaactg gcagcgagta gaaacagaac tcccttttat
4501 tgcgctgggc aacgcgctat ggcaaaaaga tttacatcaa caaaaagtgg gtggttaaat
4561 gtcgatgaat ttattgcctt ggcgtactta tcaacatcaa aagcgtttac gtcgtttagc
4621 tttttatatc gctttattta tcttgcttgc tattaattta atgttggctt ttagcaattt
4681 gattgaacaa cagaaacaaa atttgcaggc acagcaaaag tcgtttgaac aacttaatca
4741 acagcttcat aaaactacca tgcaaattga tcagttacgc attgcggtga aagttggtga
4801 agttttgaca tctattccca acgagcaagt aaaaaagagt ttacaacagc taagtgaatt
4861 accttttcaa caaggagaac tgaataaatt taaacaagat gccaataact taagcttgga
4921 aggtaacgcg caagatcaaa cagaatttga actgattcat caatttttaa agaaacattt
4981 tcccaatgtg aaattaagtc aggttcaacc tgaacaagat acattgtttt ttcactttga
5041 tgtggaacaa ggggcggaaa aatgaaagct ttttttaacg atccttttac tccttttgga
5101 aaatggctaa gtcagccttt ttatgtgcac ggtttaacct ttttattgct attaagtgcg
5161 gtgatttttc gccccgtttt agattatata gaggggagtt cacgtttcca tgaaattgaa
5221 aatgagttag cggtgaaacg ttcagaattg ttgcatcaac agaaaatttt aacctcttta
5281 caacagcagt cggaaagtcg aaaactttct ccagaactgg ctgcacaaat tattcctttg
5341 aataaacaaa ttcaacgttt agctgcgcgt aacggtttat ctcagcattt acgttgggaa
5401 atggggcaaa agcctatttt gcatttacag cttacaggtc attttgaaaa aacgaagaca
5461 tttttatccg cacttttggc taattcgtca cagctttctg taagtcggtt gcaatttatg
5521 aaacccgaag acggcccatt gcaaaccgag atcatttttc agctagataa ggaaacaaaa
5581 tgaaacattg gtttttcctg attatattat tttttatgaa ttgcagttgg ggacaagatc
5641 ctttcgataa aacacagcgt aaccgttctc agtttgataa cgcacaaaca gtaatggagc
5701 aaacagaaat aatttcctca gatgtgccta ataatctatg cggagcggat gaaaatcgcc
5761 aagcggctga aattcctttg aacgctttaa aattggtggg ggtagtgatt tctaaagata
5821 aagcctttgc cttgttgcaa gatcaaggtt tgcaagttta cagcgtttta gagggcgttg
5881 atgtggctca agagggctat attgtagaaa aaatcaacca aaacaatgtt caatttatgc
5941 gtaagctagg agagcaatgt gatagtagtg aatggaaaaa attaagtttt taaaggaaga
6001 ttatgaagaa atatttttta aagtgcggtt attttttagt atgtttttgt ttgccattaa
6061 tcgtttttgc taatcctaaa acagataacg aacgtttttt tattcgttta tcgcaagcac
6121 ctttagctca aacactggag caattagctt ttcaacaaga tgtgaattta gtgattggag
6181 atatattgga aaacaagatc tctttgaaat taaacaatat tgatatgcca cgtttgctac
6241 aaataatcgc aaaaagtaag catcttactt tgaataaaga tgatgggatt tattatttaa
6301 acggcagtca atctggcaaa ggtcaggttg caggaaatct tacgacaaat gaaccgcact
6361 tagtgagtca cacggtaaaa ctccattttg ctaaagcttc tgaattaatg aaatccttaa
6421 caacaggaag tggctctttg ctttctcccg ctgggagcat tacctttgat gatcgcagta
6481 atttgctggt tattcaggat gaacctcgtt ctgtgcaaaa tatcaaaaaa ctgattgctg
6541 aaatggataa gcctattgaa cagatcgcta ttgaagcgcg aattgtgaca attacggatg
6601 agagtttgaa agaacttggc gttcggtggg ggatttttaa tccaactgaa aatgcaagac
6661 gagttgcggg cagccttaca ggcaatagct ttgaaaatat tgcggataat cttaatgtaa
6721 attttgcgac aacgacgaca cctgctggct ctatagcatt acaagtcgcc aaaattaatg
6781 ggcgattgct tgatttagaa ttgagtgcgt tggagcgtga aaataatgta gaaattattg
6841 caagccctcg cttactcact accaataaga aaagtgcgag cattaaacag gggacagaaa
6901 ttccttacat cgtgagtaat actcgtaacg atacgcaatc tgtggaattt cgtgaggcgg
6961 tgcttggttt ggaagtgacg ccacatattt ctaaagataa caatatctta cttgatttat
7021 tggtaagtca aaattcccct ggttctcgtg tcgcttatgg acaaaatgag gtggtttcta
7081 ttgataaaca agaaattaat actcaggttt ttgccaaaga tggggaaacc attgtgcttg
7141 gcggcgtatt tcacgataca atcacgaaaa gcgaagataa agtgccattg cttggcgata
7201 tacccgttat taaacgatta tttagcaaag aaagtgaacg acatcaaaaa cgtgagctag
7261 tgattttcgt cacgccacat attttaaaag caggagaaaa cgttagaggc gttgaaacaa
7321 aaaagtgagg gtaaaaaata actttttaaa tgatgaattt ttttaatttt cgctgtatcc
7381 actgtcgtgg caatcttcat atcgcaaaaa atgggttatg ttcaggttgc caaaaacaaa
7441 ttaaatcttt tccttattgc ggtcattgtg gttcggaatt gcaatattat gcgcagcatt
7501 gtgggaattg tcttaaacaa gaaccaagtt gggataagat ggtcattatt gggcattata
7561 ttgaacctct ttcgatattg attcagcgtt ttaaatttca aaatcaattt tggattgacc
7621 gcactttagc tcggctttta tatcttgcgg tacgtgatgc taaacgaacg catcaactta
7681 aattgccaga ggcaatcatt ccagtgcctt tatatcattt tcgtcagtgg cgacggggtt
7741 ataatcaggc agatttatta tctcagcaat taagtcgttg gctggatatt cctaatttga
7801 acaatatcgt aaagcgtgtg aaacacacct atactcaacg tggtttgagt gcaaaagatc
7861 gtcgtcagaa tttaaaaaat gccttttctc ttgctgtttc gaaaaatgaa tttccttatc
7921 gtcgtgttgc gttggtggat gatgtgatta ctactggttc tacactcaat gaaatctcaa
7981 aattgttgcg aaaattaggt gtggaggaga ttcaagtgtg ggggctggca cgagcttaat
8041 ataaagcact ggaaaaaaaa gcgcgataag cgtattattc ccgatacttt ctctcaagta
8101 tttaggacat aattatggaa caagcaaccc agcaaatcgc tatttctgat gccgcacaag
8161 cgcattttcg aaaactttta gacacccaag aagaaggaac gcatattcgt attttcgcgg
8221 ttaatcctgg tacgcctaat gcggaatgtg gcgtatctta ttgccccccg aatgccgtgg
8281 aagaaagcga tattgaaatg aaatataata ctttttctgc atttattgat gaagtgagtt
8341 tgcctttctt agaagaagca gaaattgatt atgttaccga agagcttggt gcgcaactga
8401 ccttaaaagc accgaatgcc aaaatgcgta aggtggctga tgatgcgcca ttgattgaac
8461 gtgttgaata tgtaattcaa actcaaatta acccacagct tgcaaatcac ggtggacgta
8521 taaccttaat tgaaattact gaagatggtt acgcagtttt acaatttggt ggtggctgta
8581 acggttgttc aatggtggat gttacgttaa aagatggggt agaaaaacaa cttgttagct
8641 tattcccgaa tgaattaaaa ggtgcaaaag atataactga gcatcaacgt ggcgaacatt
8701 cttattatta gtgagttata aaagaagatt tataatgacc gcacttttga aagtgcggtt
8761 atttttatgg agaaaaaatg aaaatacttc aacaagatga ttttggttat tggttgctta
8821 cacaaggttc taatctgtat ttagtgaata atgaattgcc ttttggtatc gctaaagata
8881 ttgatttgga aggattgcag gcaatgcaaa ttggggaatg gaaaaattat ccgttgtggc
8941 ttgtggctga gcaagaaagt gatgaacgag aatatgtgag tttgagtaac ttgctttcac
9001 tgccagagga tgaattccat atattaagcc gaggtgtgga aattaatcat tttctgaaaa
9061 cccataaatt ctgtggaaag tgcggtcata aaacacaaca
//