GenBank-Updates@genbank.bio.net (05/14/91)
LOCUS SYNYSCECOP 233 bp ds-DNA SYN 14-MAY-1991 DEFINITION S.cerevisiae DNA fragment with promoter activity fused to E.coli beta-galactosidase gene. ACCESSION M64434 KEYWORDS fusion protein. SOURCE S.cerevisiae (strain FL 100) DNA, and E.coli DNA. ORGANISM Saccharomyces cerevisiae Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes; Endomycetales; Saccharomycetaceae. REFERENCE 1 (bases 1 to 233) AUTHORS Kormanec,J. TITLE A yeast chromosomal fragment having promoter activity in E.coli JOURNAL Unpublished (1991) STANDARD full staff_entry FEATURES Location/Qualifiers -35_signal 14..19 /note="-35 region of E.coli consensus" misc_feature 20..35 /note="regular 16 bp promoter spacing" -10_signal 36..41 /note="-10 region of E.coli consensus" RBS 183..187 /note="region homologous to E.coli RBS sequence" CDS 194..233 /product="beta galactosidase fusion protein" /codon_start=194 /partial misc_feature 224..229 /note="XbaI fusion site with beta-galactosidase" /product="beta galactosidase fusion protein" /partial BASE COUNT 71 a 31 c 47 g 84 t ORIGIN 1 aattgatgga ttcttgacac tctcaaaaag gtcattataa tctttggtag gtattatcag 61 gcaatcaatt ttggatgtta aaggcagtaa aaaatcaata gtaggctctt ttaacaagtt 121 aaacgtactt tgatcgatgg gtatcttttt caaagcctga agtttttcct tgagatagtt 181 atatgatgga tttgataggg attcgtttag gttctcaaac tcctctagag gat //