GenBank-Updates@genbank.bio.net (05/14/91)
LOCUS SYNYSCECOP 233 bp ds-DNA SYN 14-MAY-1991
DEFINITION S.cerevisiae DNA fragment with promoter activity fused to E.coli
beta-galactosidase gene.
ACCESSION M64434
KEYWORDS fusion protein.
SOURCE S.cerevisiae (strain FL 100) DNA, and E.coli DNA.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 233)
AUTHORS Kormanec,J.
TITLE A yeast chromosomal fragment having promoter activity in E.coli
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
-35_signal 14..19
/note="-35 region of E.coli consensus"
misc_feature 20..35
/note="regular 16 bp promoter spacing"
-10_signal 36..41
/note="-10 region of E.coli consensus"
RBS 183..187
/note="region homologous to E.coli RBS sequence"
CDS 194..233
/product="beta galactosidase fusion protein"
/codon_start=194
/partial
misc_feature 224..229
/note="XbaI fusion site with beta-galactosidase"
/product="beta galactosidase fusion protein"
/partial
BASE COUNT 71 a 31 c 47 g 84 t
ORIGIN
1 aattgatgga ttcttgacac tctcaaaaag gtcattataa tctttggtag gtattatcag
61 gcaatcaatt ttggatgtta aaggcagtaa aaaatcaata gtaggctctt ttaacaagtt
121 aaacgtactt tgatcgatgg gtatcttttt caaagcctga agtttttcct tgagatagtt
181 atatgatgga tttgataggg attcgtttag gttctcaaac tcctctagag gat
//