GenBank-Updates@genbank.bio.net (05/14/91)
LOCUS BUTGLGB 2500 bp ds-DNA BCT 14-MAY-1991
DEFINITION Butyrivibrio fibrisolvens 1,4-glucan:1,4-glucan
6-glycosyltransferase (glgB) gene, complete cds.
ACCESSION M64980
KEYWORDS 1,4-glucan:1,4-glucan 6-glycosyltransferase; branching enzyme.
SOURCE Butyrivibrio fibrisolvens (strain H17c) DNA.
ORGANISM Butyrivibrio fibrisolvens
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Anaerobic gram-negative straight, curved and helical rods;
Bacteroidaceae.
REFERENCE 1 (bases 1 to 2500)
AUTHORS Rumbak,E., Rawlings,D.E., Lindsay,G.G. and Woods,D.R.
TITLE Characterization of a glgB gene from Butyrivibrio fibrisolvens
encoding a glycogen branching enzyme with starch clearing activity
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS <1..404
/note="unidentified partial ORF"
/evidence=EXPERIMENTAL
/codon_start=3
RBS 426..432
/gene="glgB"
CDS 439..2358
/function="branching enzyme"
/EC_number="2.4.1.18"
/product="1,4-glucan:1,4-glucan 6 glycosyltransferase"
/gene="glgB"
/evidence=EXPERIMENTAL
/codon_start=439
terminator 2379..2418
/gene="glgB"
BASE COUNT 778 a 440 c 603 g 679 t
ORIGIN
1 gaattcttca ggaagagaac aaggggctca ttattatcat ggaagatacc aaggaagata
61 tggacagatt ccttgataag aatccatcac ttaagcagag ctttaatgtc agagttgata
121 ttcaggctct ggatgatgat tcacttgttg cttatgcaag gcagtatgct tatgaaaaag
181 agtatgctat tgataatctc ggtatacttg ctcttcatac cagaatttca gagaggcaga
241 ctcttgacca tgaggtgaca gttgcagagg tcaaggatat tgtggatgat gctatctact
301 acgcagagaa gcgctcagtt gctcactttg tggatgttct tgtcaacaag agatatgatg
361 agaatgacat ggtcatctta agagaaaaag attttatgcg ctaaggaata agtaacaaat
421 aaactgaggg ggttatttat gagtcaaaag gtttttatat ctgaggacga cgagtactta
481 tttggacagg gcacacatta cgatatttat gataaattgg gagctcatcc atcagaagaa
541 aagggcaaaa aaggattctt ttttgcagta tgggcaccaa atgctgcaga tgtgcatgta
601 gtaggtgact ttaatggttg ggatgaaaat gcccatcaaa tgaagaggag caaaacaggt
661 aacatctgga ctttgttcat tccgggggta gcaataggag ctttatacaa attcctcatt
721 acagctcagg atggaagaaa actttacaag gctgatcctt atgcgaatta tgcagaactt
781 aggccgggta atgcttccag aacaacagat ctttcaggct ttaagtggtc agattccaag
841 tggtatgaat cacttaaggg taaagatatg aaccgtcagc ctatagccat ctatgaatgc
901 catataggct catggatgaa acatcctgat ggcacagagg acggtttcta tacatataga
961 caattcgctg acagaattgt ggaatacctc aaggagatga agtatacaca tatagagctg
1021 attggaatag ctgagcatcc ttttgatgga tcctggggct atcaggttac tggctactat
1081 gctcctacag ccagatatgg cgagccaaca gactttatgt atctgatcaa ccagctccac
1141 aagcatggaa tcggcgtaat tctcgattgg gttccggccc atttctgccc ggatgaattt
1201 ggtcttgcat gctttgacgg aacatgtatt tatgaagatc cggatcctcg caagggagaa
1261 catcctgact ggggaaccaa gatattcaat ctggccaagc cggaagtcaa gaacttcctt
1321 atcgccaatg ctttatactg gatccgcaag ttccatattg atggacttag ggtagatgca
1381 gttgcttcaa tgctctatct tgattatggc aaaaaagatg gacagtgggt tccaaataaa
1441 tatggagata acaagaacct cgatgctatc gagttcttta aacattttaa cagcgtagta
1501 aggggaacat accctaatat tctcactata gctgaggagt ctacagcgtg gcccaaggtt
1561 actgctccgc cagaggagga tggtcttggt tttgcgttca agtggaacat gggatggatg
1621 catgacttct gcgaatacat gaagcttgat ccatacttta gacagggtgc tcattacatg
1681 atgacttttg caatgagcta caatgattca gagaattaca ttttgccatt gtctcacgac
1741 gaggtcgtac accttaagtg ttcaatggta gagaagatgc caggatacaa ggttgataaa
1801 tatgctaacc taagagttgg ttatacctac atgtttggtc actcaggtaa aaagctcctc
1861 tttatgggac aggattttgg tcaggaaaga gaatggagcg agaagagaga acttgactgg
1921 ttcctcttgg agaacgacct taacagagga atgaaggact atgtaggtaa acttctggaa
1981 atatacagaa agtatcctgc tctctatgaa gtagataatg actggggcgg ctttgagtgg
2041 ataaatgccg acgacaagga gcgcagcacc tatagtttct accgtagggc atctaatggc
2101 aaggacaata ttctctttgt tcttaatatg acaccaatgg agagaaaggg ctttaaggta
2161 ggtgttccat ttgatggaac ctatacaaag attctcgaca gtgccaagga gtgctatggc
2221 ggcagtggca gtagcgttcc cgataagatc aaggcagtaa aaggtctgtg tgattacaag
2281 gattacagca tagaatttga tcttccgcct tacggcgcag aagtatttgt tttccagacg
2341 aagaaaacaa agaattaaag attttaaaaa ctaagccgaa ataaatggtg atggacaata
2401 tgttcatcac cattttttgt gtgataaaaa tagtgaggta caaatgaata ttaactttca
2461 ggcaattacc ggccacggca aggacgagag tttggatatc
//