GenBank-Updates@genbank.bio.net (05/14/91)
LOCUS TFEREPB 1400 bp ds-DNA BCT 14-MAY-1991
DEFINITION Plasmid pTF-FC2 replication primase (repB) gene, complete cds.
ACCESSION M64981
KEYWORDS replication primase.
SOURCE Plasmid pTF-FC2 (from Thiobacillus ferrooxidans) DNA.
ORGANISM Plasmid pTF-FC2
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 1400)
AUTHORS Dorrington,R.A., Bardien,S. and Rawlings,D.E.
TITLE The broad-host-range plasmid, pTF-FC2, requires a primase-like
protein for autonomous replication in Escherichia coli
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
RBS 200..205
/gene="repB"
CDS 217..1275
/product="replication primase"
/gene="repB"
/codon_start=217
BASE COUNT 337 a 428 c 455 g 180 t
ORIGIN
1 atcgatattc acgactggcg caaccgggac agcacgcttg ccgccttgca gctatcggcg
61 cagaagtggg gcagcttcac cgtaaccggg aacgacgaat acaaggccat gtgcgggaag
121 ttagcagcag agcacggctt caagatcaca aatgcagagc ttcaggagag catccagcag
181 gagcggcagc ggatacagca ggagagggcg caggcgatga aatcggagca gctaaagcag
241 ttcgagcggt acgcggaagc ggtaggcgcg gagcgctacc gggtaacgtc catcaagatg
301 caggcagacg gaaggaagca aaccttcatc ctcgacaaga aggacggcat cacgcggggt
361 ttcacaccgc aggagatcga gcagcgcacg ccggagatgc agcgcctaca gcgccggggc
421 gaaaacctct actacacgcc gctatcggac aagaagcatc acatcctcat cgacgacatg
481 aaccgggaga agctggagcg gcttatcaaa gacggctacc agcccgccgc cgtgctggaa
541 tccagccccg gcaactatca ggccatcatc accgtgtcga agctggggac ggcccacgat
601 aaggacgtgg gcaaccgcct gagcgatgcc ctgaaccgtg aatacggcga cccgaagcta
661 tcgggagcca tccacccgca ccgcgcaccc ggctacgaga accgcaagcc caagcaccag
721 cgggaggacg gcagctatcc agaggtgcgc ttgctcaagg ccgagcgccg ggagtgcgtc
781 aaggcgctgg ccttgtccag ccagatcgac gccgagtatc agcggcaagc ggccttgaag
841 gcgcagcagc ccgagcgcac gaaagccaag cccgccttgg agcttgcagc ggccagcggc
901 agcgcgatcg acgcctacca gcggcattac cgcgacgtgc tcaagcggca gcgtggcggc
961 gaggtggact tgtcccgcgt ggattccatg attgccgtgc gtatgcgcgt caccggccac
1021 gatcaagcgg ccatcgaggg cgctatccgc cagtgcgcac cggccacccg gcagaaagac
1081 gagggccgcg attggaacga ctacgcgcag cgcaccgccc gctatgccta cagcgccgca
1141 cagccgcaag ccgccgatct tggcaagtac cggcagcagt gggagaagct ggaagggcgc
1201 gagcctgtac gacagcagga gcaggcaaag gcgcagaaga tcgagcgcga caactcgccg
1261 ggaatgagtc tctagcgttg cgtggtggtt gtgatatact tgtatagcgt tttcagaaca
1321 ggagccgaaa catgcttgca atccgactgc ccgccgaagt ggaaacccgc cttgaagcac
1381 tggcgcaggc cacagggcgg
//