GenBank-Updates@genbank.bio.net (10/25/90)
LOCUS ECORIBI34 1206 bp ds-DNA BCT 25-OCT-1990
DEFINITION E.coli ribonuclease I gene, complete cds.
ACCESSION M55687 M37486
KEYWORDS ribonuclease I.
SOURCE Escherichia coli (K12) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1206)
AUTHORS Meador,J.III. and Kennell,D.
TITLE Cloning and sequencing the gene encoding Escherichia coli
ribonuclease I: Exact physical mapping using the genome library
JOURNAL Gene (1990) In press
STANDARD simple staff_entry
FEATURES from to/span description
pept 250 1056 ribonuclease I signal precursor
/EC_number="3.1.27.6"
sigp 250 318 ribonuclease I signal peptide
matp 319 1053 ribonuclease I
BASE COUNT 313 a 296 c 289 g 308 t
ORIGIN
1 ggtcctgggg tgattattta cggctgtggc tatgtgaaat caaaagatta ctggcgtctt
61 ggctgcaatc ttcggggtga tttacatctc tatgttgctg ttggttggct ggccgattct
121 cgccatgtgg aactaatacg ttcgccaaac gctgaataaa tcatcacgcc cgccaggtaa
181 gcccacctgg cgggcttttt tatgatttaa tagatagtat tcagtgtatg tatgagttcc
241 acacccatta tgaaagcatt ctggcgtaac gccgcgttgc tcgcggtttc tctgcttccc
301 ttctcttctg ccaacgcctt agcgttgcag gcaaaacagt atggcgattt tgatcgctat
361 gtcctggccc tctcctggca aaccggattt tgccagagtc aacacgatcg aaatcgtaac
421 gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt tctgaccgta
481 catggtctgt ggccaggatt gcctaaatcg gttgctgccc gtggtgttga tgaacgccgc
541 tggatgcgct tcggttgcgc tactcgccca atcccgaatc taccagaagc gcgcgccagc
601 cgaatgtgtt catcgccgga aaccggatta tcactggaaa cggccgctaa actaagtgaa
661 gtcatgccag gagctggcgg acgttcctgc ctggaacgct acgaatatgc caaacacggt
721 gcctgctttg gttttgatcc ggacgcatac ttcggtacga tggtacgcct gaatcaagaa
781 attaaagaga gcgaagccgg aaaattcctt gcggataatt acggtaaaac agtgagccgc
841 cgtgactttg acgccgcctt tgccaaaagc tggggaaaag agaacgtgaa agcagttaag
901 ctaacgtgcc agggtaaccc tgcgtatttg actgaaattc agatctcgat caaagctgac
961 gccatcaacg ctccgctttc tgcaaactca ttcttgccac aacctcaccc aggtaactgt
1021 ggcaaaacct ttgtgattga taaagcgggt tattaatcat ctgatttaca aaacacgcta
1081 attcgtcttc cataaaccaa ggcgattaag atttatacat aatgcgctgc attgaggaat
1141 tccctgaatg tgacgcaaat cacttcaaag agtgagcaac agtcagtatc atcatgtgag
1201 ttaaac
//GenBank-Updates@genbank.bio.net (11/14/90)
LOCUS ECORIBI34 1206 bp ds-DNA BCT 14-NOV-1990
DEFINITION E.coli ribonuclease I gene, complete cds.
ACCESSION M55687 M37486
KEYWORDS ribonuclease I.
SOURCE Escherichia coli (K12) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1206)
AUTHORS Meador,J.III. and Kennell,D.
TITLE Cloning and sequencing the gene encoding Escherichia coli
ribonuclease I: Exact physical mapping using the genome library
JOURNAL Gene (1990) In press
STANDARD simple staff_entry
FEATURES from to/span description
pept 250 1056 ribonuclease I signal precursor
/EC_number="3.1.27.6"
sigp 250 318 ribonuclease I signal peptide
matp 319 1053 ribonuclease I
BASE COUNT 313 a 296 c 289 g 308 t
ORIGIN 14.3 min on the 100 min. genetic map.
1 ggtcctgggg tgattattta cggctgtggc tatgtgaaat caaaagatta ctggcgtctt
61 ggctgcaatc ttcggggtga tttacatctc tatgttgctg ttggttggct ggccgattct
121 cgccatgtgg aactaatacg ttcgccaaac gctgaataaa tcatcacgcc cgccaggtaa
181 gcccacctgg cgggcttttt tatgatttaa tagatagtat tcagtgtatg tatgagttcc
241 acacccatta tgaaagcatt ctggcgtaac gccgcgttgc tcgcggtttc tctgcttccc
301 ttctcttctg ccaacgcctt agcgttgcag gcaaaacagt atggcgattt tgatcgctat
361 gtcctggccc tctcctggca aaccggattt tgccagagtc aacacgatcg aaatcgtaac
421 gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt tctgaccgta
481 catggtctgt ggccaggatt gcctaaatcg gttgctgccc gtggtgttga tgaacgccgc
541 tggatgcgct tcggttgcgc tactcgccca atcccgaatc taccagaagc gcgcgccagc
601 cgaatgtgtt catcgccgga aaccggatta tcactggaaa cggccgctaa actaagtgaa
661 gtcatgccag gagctggcgg acgttcctgc ctggaacgct acgaatatgc caaacacggt
721 gcctgctttg gttttgatcc ggacgcatac ttcggtacga tggtacgcct gaatcaagaa
781 attaaagaga gcgaagccgg aaaattcctt gcggataatt acggtaaaac agtgagccgc
841 cgtgactttg acgccgcctt tgccaaaagc tggggaaaag agaacgtgaa agcagttaag
901 ctaacgtgcc agggtaaccc tgcgtatttg actgaaattc agatctcgat caaagctgac
961 gccatcaacg ctccgctttc tgcaaactca ttcttgccac aacctcaccc aggtaactgt
1021 ggcaaaacct ttgtgattga taaagcgggt tattaatcat ctgatttaca aaacacgcta
1081 attcgtcttc cataaaccaa ggcgattaag atttatacat aatgcgctgc attgaggaat
1141 tccctgaatg tgacgcaaat cacttcaaag agtgagcaac agtcagtatc atcatgtgag
1201 ttaaac
//GenBank-Updates@genbank.bio.net (05/15/91)
LOCUS ECORIBI34 1206 bp ds-DNA BCT 15-MAY-1991
DEFINITION E.coli ribonuclease I gene, complete cds.
ACCESSION M55687 M37486
KEYWORDS ribonuclease I.
SOURCE Escherichia coli K12 (strain W3110) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1206)
AUTHORS Meador,J.III. and Kennell,D.
TITLE Cloning and sequencing the gene encoding Escherichia coli
ribonuclease I: Exact physical mapping using the genome library
JOURNAL Gene (1991) In press
STANDARD simple staff_entry
FEATURES Location/Qualifiers
sig_peptide 250..318
/EC_number="3.1.27.6"
/product="ribonuclease I"
/gene="rna"
/codon_start=250
CDS 250..1056
/EC_number="3.1.27.6"
/note="on the 100 min. genetic map"
/product="ribonuclease I"
/gene="rna"
/codon_start=250
mat_peptide 319..1053
/EC_number="3.1.27.6"
/product="ribonuclease I"
/gene="rna"
/codon_start=319
BASE COUNT 313 a 296 c 289 g 308 t
ORIGIN
1 ggtcctgggg tgattattta cggctgtggc tatgtgaaat caaaagatta ctggcgtctt
61 ggctgcaatc ttcggggtga tttacatctc tatgttgctg ttggttggct ggccgattct
121 cgccatgtgg aactaatacg ttcgccaaac gctgaataaa tcatcacgcc cgccaggtaa
181 gcccacctgg cgggcttttt tatgatttaa tagatagtat tcagtgtatg tatgagttcc
241 acacccatta tgaaagcatt ctggcgtaac gccgcgttgc tcgcggtttc tctgcttccc
301 ttctcttctg ccaacgcctt agcgttgcag gcaaaacagt atggcgattt tgatcgctat
361 gtcctggccc tctcctggca aaccggattt tgccagagtc aacacgatcg aaatcgtaac
421 gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt tctgaccgta
481 catggtctgt ggccaggatt gcctaaatcg gttgctgccc gtggtgttga tgaacgccgc
541 tggatgcgct tcggttgcgc tactcgccca atcccgaatc taccagaagc gcgcgccagc
601 cgaatgtgtt catcgccgga aaccggatta tcactggaaa cggccgctaa actaagtgaa
661 gtcatgccag gagctggcgg acgttcctgc ctggaacgct acgaatatgc caaacacggt
721 gcctgctttg gttttgatcc ggacgcatac ttcggtacga tggtacgcct gaatcaagaa
781 attaaagaga gcgaagccgg aaaattcctt gcggataatt acggtaaaac agtgagccgc
841 cgtgactttg acgccgcctt tgccaaaagc tggggaaaag agaacgtgaa agcagttaag
901 ctaacgtgcc agggtaaccc tgcgtatttg actgaaattc agatctcgat caaagctgac
961 gccatcaacg ctccgctttc tgcaaactca ttcttgccac aacctcaccc aggtaactgt
1021 ggcaaaacct ttgtgattga taaagcgggt tattaatcat ctgatttaca aaacacgcta
1081 attcgtcttc cataaaccaa ggcgattaag atttatacat aatgcgctgc attgaggaat
1141 tccctgaatg tgacgcaaat cacttcaaag agtgagcaac agtcagtatc atcatgtgag
1201 ttaaac
//