GenBank-Updates@genbank.bio.net (05/15/91)
LOCUS PWWXYL 9037 bp ds-DNA BCT 15-MAY-1991
DEFINITION Pseudomonas putida plasmid pWW0 meta operon, 5' genes.
ACCESSION M64747
KEYWORDS 1,2-dihydroxycyclohexa-3,4-diene carboxylate;
2-hydroxymuconic semialdehyde dehydrogenase;
2-hydroxymuconic semialdehyde hydrogenase;
2-hydroxypent-2,4-dienoate hydratase; catechol 2,3-dioxygenase;
chloroplast-type ferredoxin; meta operon; toluate 1,2-dioxygenase.
SOURCE Plasmid pWW0, DNA.
ORGANISM Plasmid pWW0
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 9037)
AUTHORS Harayama,S., Rekik,M., Bairoch,A., Neidle,E.L., Hartnett,C. and
Ornston,L.N.
TITLE DNA sequence repetitions acquired during evolutionary divergence of
Acinetobacter calcoaceticus chromosomal benABC and Pseudomonas
xylXYZ, genes encoding benzoate dioxygenases
JOURNAL Unpublished (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
mRNA 445..>9037
/note="meta operon"
RBS 463..466
/gene="xylX"
CDS 469..1833
/product="toluate 1,2-dioxygenase subunit"
/gene="xylX"
/codon_start=469
RBS 1821..1824
/gene="xylY"
CDS 1834..2322
/product="toluate 1,2-dioxygenase subunit"
/gene="xylY"
/codon_start=1834
RBS 2321..2324
/gene="xylZ"
CDS 2332..3342
/product="toluate 1,2-dioxygenase subunit"
/gene="xylZ"
/codon_start=2332
RBS 3447..3450
/gene="xylL"
CDS 3457..4266
/product="1,2-dihydroxycyclohexa-3,4-diene carboxylate
dehydrogenase"
/gene="xylL"
/codon_start=3457
RBS 4403..4405
/gene="xylT"
CDS 4411..4749
/note="chloroplast-type ferredoxin"
/gene="xylT"
/codon_start=4411
RBS 4735..4738
/gene="xylE"
CDS 4746..5669
/EC_number="1.13.11.12"
/product="catechol 2,3-dioxygenase"
/gene="xylE"
/codon_start=4746
RBS 5691..5694
/gene="xylG"
CDS 5704..7164
/product="2-hydroxymuconic semialdehyde dehydrogenase"
/gene="xylG"
/codon_start=5704
RBS 7163..7167
/gene="xylF"
CDS 7175..8020
/product="2-hydroxymuconic semialdehyde hydrogenase"
/gene="xylF"
/codon_start=7175
RBS 8022..8025
/gene="xylJ"
CDS 8032..8700
/product="2-hydroxypent-2,4-dienoate hydratase"
/gene="xylJ"
/codon_start=8032
BASE COUNT 1803 a 2796 c 2790 g 1648 t
ORIGIN
1 taacgacgct ggtcgtcctc gtgcggctgc tggtgctgtg ctgaccctgc cgctgtcctg
61 atcggcctcg tcggcctcgt ggacggcctg tcgccggaca tcgcgcttcg gcgcgggacg
121 cgagtcgggg ttcatctatc accgcgccag ggccagcctg atcccgctgg ccgtactgcc
181 gtgggtgact tacctggcac tgccggtcag cgtgaacccg ctgctgatcc tgctgcccag
241 cgccgcactg ctcggcgtgg cggtgtgcat cgctgcggcg acgttcaaga agtatctgta
301 ggtgcgacac gttaattttg atcagcagtt ccttaacggc tgcagtgtcc ggtttgatag
361 ggataagtcc agccttgcaa gaagcggata caggagtgca aaaaatggct atctctagaa
421 aggcctaccc cttaggcttt atgcaacaga aacaataata atggagtcat gaccatgaca
481 atgcacctgg ggctcgacta tatagatagt ctcgttgaag aagatgagaa cgagggcatc
541 taccgctgca agcgcgagat gttcaccgac cctcggctgt tcgatttaga gatgaaacac
601 atctttgagg gcaactggat ttatctcgcc cacgagagcc agattcccga gaagaacgac
661 tattacacca cgcagatggg ccggcagccg atattcatca cacgcaacaa agatggtgag
721 ctgaatgcct tcgtcaatgc ctgcagtcac cgcggcgcca cgctctgtcg ctttaggagt
781 ggaaacaagg ccacccacac ctgctcgttc cacggctgga ccttcagcaa ttcgggcaag
841 ctgctcaagg tcaaagaccc caagggtgcc ggctatccgg acagcttcga ctgtgacggc
901 tcgcacgacc tgaagaaagt tgcgcgcttt gcttcctacc gcggatttct attcggcagc
961 ctgcgcgagg acgtcgcccc gttggaagag ttcctcggcg agtcgaggaa ggtcatcgac
1021 atggtcgtcg accagtcgcc cgaaggtctg gaagtgctgc gcggttccag tacctatgtt
1081 tacgaaggca actggaaagt gcaggtcgag aacggtgccg acggctacca cgtcagtact
1141 gttcactgga actacgccgc cacccagcag cagcgcaagc tgagagacgc gggcgatgat
1201 attcgcgcca tgaccgccag tagctggggc ggggatggcg gcggtttcta ctcctttgaa
1261 aacggccacc agatggtctg ggcacgctgg ggtgacccga aaaaccgccc gctgttcgcc
1321 gagcgagatc gcttagccag cgagtttggt gaagcccgtg ccgactggat gatcggcgtc
1381 tcccgcaacc tctgcctcta cccgaacctc tacctgatgg accagttcgg ctcgcagttg
1441 cgtatcaccc gtccgctgtc ggtggataga accgaaatca ccatctactg catcgcgccc
1501 aaaggcgaaa cgccgaggcg tgcccgccgt gtccgtcagt acgaggactt cttcaatgtc
1561 agcggcatgg ccaccccgga cgacctggag gaattccgcg cctgccagga gggcttcgcc
1621 ggcgggggga tgaacgacat gtcccgtggc gccaaacact ggatcgaggg gccggacgag
1681 ggcgcgaagg agatcgatct gcatccgaag ctgagcggtg tccgctcgga agacgaaggc
1741 ctgttcgtca tgcagcacaa gtactggcaa cagcagatga tcaaggccgt caagcgagaa
1801 caggatcggc tgatccatgc ggagggcgtg taaatgacta tctcctacga agccgtgcgc
1861 gatttccttt accgcgaagc acgctacctc gacgacaagc agtgggaaag ctggctggaa
1921 atgtacgcgc cggacgccac tttctggatg ccggcctggg acgaccgcga ccaattgacc
1981 gaagacccgc agagccagat ctcgctgatt tggtacggca atcgcagtgg cctagaggat
2041 cgggtgttcc gcatcaagac cgagcgttcc agtgccacca ttccggacac ccgaaccagc
2101 cacaacatca gcaatttgga gttgctcgag cagtccgatg gcgtctgtaa gctgcgctac
2161 aactggcaca ccatgaatta tcggtacaag acggtggacc acttctttgg caccaatttc
2221 tgcactctcg acacatgcgg cgagaccccg ctgattacgg ccaagaaggt cgtgctgaag
2281 aacgactaca tccgccaagt tatcgatgta taccacgtct gaggtgccgc catgacacac
2341 aaggttgcca ctgacttcga agacggcgtc actcgtttca tcgatgctaa tactggcgag
2401 actgttgctg atgcagccta ccgccaaggc atcaatttac ccctggactg ccgagacggt
2461 gcatgcggcg cctgcaaatg cttcgctgag agcggccgct acagcctcgg cgaggagtat
2521 atcgaggatg cacttagcga agccgaagcc gagcagggct acgtgctgac ctgccagatg
2581 cgcgccgaaa gcgactgcgt gattcgcgtt ccggccgcat cggacgtctg caagacccag
2641 caggccggct atcaggcagc gatcagcaac gtgcgtcagc tgtccgagag caccatcgcg
2701 ctgtctatca aaagcgcatc gctgaaccag ttggcgttcc tgccaggcca gtacgtcaat
2761 ctgcaagtgc cgggcagcga ccagacccgc gcctattcct tcagctcgct gcaaaaggat
2821 ggcgaggtca gcttcctgat ccgcaagctg ccgggcgggc tgatgagcag cttcctgacc
2881 agcctcgcca aggtcggcga tagcgtcagt ctggctggac cgctgggcgc gttctatctg
2941 cgcgagatca agcggccgct gctgttgctg gcgggcggta ccggcctagc gccgttcacc
3001 gcgatgctgg agaagatcgc cgagcagggc ggcgagcacc cgctccatct gatctacggc
3061 gtcacccatg accacgacct ggttgaaatg gacaagctag aggcattcgc cgcgcgcatt
3121 cccaacttca gctacagcgc ctgcgtcgcc agcccagaca gcgcctatcc gcaaaagggc
3181 tatgtgaccc agtacatcga gcccaaacag ctcaacggcg gtgaggtaga tatctacctt
3241 tgcggtccgc caccgatggt cgaggcggtc agtcagtaca tccgggcaca gggcatccag
3301 ccggccaatt tctattatga gaagttcgcc gccagcgcct agaggctcct ttggtccgct
3361 tactaggtga gcgggcctgt tttattcggg cagtagggcc caggttctct gcgaactttc
3421 ccaaattgca tccgggctac acaaccgagg tggttcatga acaaacgttt ccagggcaag
3481 gttgccgtta tcaccggcgc cgcccagggc atcggtcgcc gcgtggccga acggatggcg
3541 gccgaaggcg gtcggctgct gctggtcgac cgttccgagc taatacatga gctggccgac
3601 gaactggtcg gagtcgctga ggtgctgacc ctgaccgccg accttgagca gttcgccgag
3661 tgccaacggg tgatggcggc ggcgctcgag cgcttcggtc gtctggatat tctgatcaac
3721 aacgttggcg gcaccatttg ggctaagcca ttcgagcatt accaggaacg cgagatcgag
3781 gccgaagtgc ggcgttcact gtttcctacc ctatggtgct gccacgccgc gctggcgccc
3841 atgatcgagc agggcagtgg cgccatcgta aatgtttcct ccgttgccac gcgcgggatc
3901 catcgcgtgc cttacggcgc ggcgaagggt ggcgttaacg cccttaccgc ctgtctggcc
3961 tttgaaaccg ccgagcgcgg catccgcgtc aacgccaccg caccgggtgg caccgaggcg
4021 cgccacggcg gattccgcaa tagcgccgag ccgagtgagc aggagaaggt ctggtaccag
4081 cagatcgtcg accagtccct cgacagcagc cttatgaaac gctacggaag cattgacgag
4141 caggtcgagg caattctgtt ccttgcttct gacgccgcct cctacatcac cggtataact
4201 cttccggtgg cagggggaga cctcggctgc cagagctgtt ccgtcatgtt tagtgtatct
4261 gggtgaccct tatgtctggt taagaagaga aatcgacatg cgaagaagca acgtacataa
4321 gacccctgag gctcattttc ggggttatgg cggcatcacc cagagctgtt gggggatact
4381 tccgtcatgt ttagtgtatc tgggatgaat atgaacagtg ccggctacga ggtgttcgaa
4441 gtgctaagcg gccagtcatt ccgctgtgcg gagggccagt cggtactgcg cgccatggaa
4501 gcccagggca agcgctgcat accggtgggc tgtcgcggtg gcggttgcgg cctttgtaga
4561 gtgcgggtgc tcagcggagc ctaccggagc ggacgcatga gccgcggtca cgtgccggcc
4621 aaggccgccg ccgaagcgct ggccctggcc tgtcaagtgt ttccgcaaac cgacttgacc
4681 atcgagtact ttcgccacgt tggcggaaac aaacctgaca acatgaacta tgaagaggtg
4741 acgtcatgaa caaaggtgta atgcgaccgg gccatgtgca gctgcgtgta ctggacatga
4801 gcaaggccct ggaacactac gtcgagttgc tgggcctgat cgagatggac cgtgacgacc
4861 agggccgtgt ctatctgaag gcttggaccg aagtggataa gttttccctg gtgctacgcg
4921 aggctgacga gccgggcatg gattttatgg gtttcaaggt tgtggatgag gatgctctcc
4981 ggcaactgga gcgggatctg atggcatatg gctgtgccgt tgagcagcta cccgcaggtg
5041 aactgaacag ttgtggccgg cgcgtgcgct tccaggcccc ctccgggcat cacttcgagt
5101 tgtatgcaga caaggaatat actggaaagt ggggtttgaa tgacgtcaat cccgaggcat
5161 ggccgcgcga tctgaaaggt atggcggctg tgcgtttcga ccacgccctc atgtatggcg
5221 acgaattgcc ggcgacctat gacctgttca ccaaggtgct cggtttctat ctggccgaac
5281 aggtgctgga cgaaaatggc acgcgcgtcg cccagtttct cagtctgtcg accaaggccc
5341 acgacgtggc cttcattcac catccggaaa aaggccgcct ccatcatgtg tccttccacc
5401 tcgaaacctg ggaagacttg cttcgcgccg ccgacctgat ctccatgacc gacacatcta
5461 tcgatatcgg cccaacccgc cacggcctca ctcacggcaa gaccatctac ttcttcgacc
5521 cgtccggtaa ccgcaacgaa gtgttctgcg ggggagatta caactacccg gaccacaaac
5581 cggtgacctg gaccaccgac cagctgggca aggcgatctt ttaccacgac cgcattctca
5641 acgaacgatt catgaccgtg ctgacctgat ggtccggtac gacttattgc agagattgtg
5701 cagatgaaag aaatcaagca tttcattagc ggtgaattag tcggttcggc cagcggcaag
5761 ctgttcgaca atgtcagccc ggccaacggc caggtgatcg gccgcgtcca cgaggccggc
5821 cgcgccgagg tcgacgccgc ggtcagggcg gcacgcgctg cgctgaaggg accctggggg
5881 aagatgacgg tggccgagcg cgctgagatt ctgcatcgcg tggccgatgg catcacggcg
5941 cgcttcggcg agtttctcga ggcccgaatg cctggacacc ggcaagccga agtcgctggc
6001 cagccacatc gacattccgc gcggcgcgcc aatttcaagg tgttcgccga cctgctcaag
6061 aatgttgcca atgaagcctt cgagatggcc accccggacg gcgccggtgc actcaactac
6121 ggcgtgcgcc ggcccaaggg ggtgatcggg gtgatcagcc cgtggaacct gccgctgctg
6181 ctgatgacct ggaaagtcgg cccggccctg gcctgcggca actgcgtggt ggtcaaacca
6241 tccgaggaaa ccccgctgac cgccaccctg ctcggcgagg tgatgcaggc cgccggtgtg
6301 ccggccggcg tgtacaacgt ggtgcacggt ttcggcggcg attcggccgg ggccttcctc
6361 accgagcacc cggacgtcga cgcctacacc ttcaccggcg agaccggcac cggcgaaacc
6421 atcatgcgcg ccgcggccaa gggcgtgcgc caggtgtcgc tggagctggg cggcaagaac
6481 gccggcatcg tcttcgccga ctgcgatatg gacaaggcca tcgagggcac cctgcgctcg
6541 gccttcgcca actgcggcca ggtctgcctg ggcaccgagc gggtgtatgt cgagcggccg
6601 atcttcgacg cgttcgtcgc ccgcctgaag gccggcgccg aagcgttgaa gatcggcgaa
6661 ccgaacgatc cagaggccaa tttcggcccg ctgatcagcc ataagccccg tgaaaaagtc
6721 cccagttact accagcaggc agtcgacgac ggcgccaccg ttgtcaccgg cggcggcgtg
6781 ccggagatgc cggcgcacct ggccggcggc gcctgggtgc agccgactat ctggaccggc
6841 ctggccgacg attcggcggt ggtcaccgag gaaatcttcg gcccctgctg ccatatccgc
6901 ccgttcgaca gcgaggagga agccattgaa ctggccaaca gcctgcctta cggcctggcc
6961 tcggcgatct ggaccgagaa cgttcgccgc gcccaccgcg tcgccgggca gattgaggcc
7021 ggcatcgtct gggtcaacag ctggttcctg cgcgacctgc gcaccgcctt cggcggcagc
7081 aagcagtcgg gcatcgggcg cgaagggggt gtgcactcgc tggagttcta caccgagctg
7141 aaaaacatct gtgtgaaact ttgaggacct ggtcatgaac gcaccgcagc aaagccctga
7201 aatcggtcgc gaaatcctcg ccgccggcta ccgcaccaac ctgcatgatc agggcgaagg
7261 cttcccggct ctgctgatcc acggctccgg ccccgcgtca ccgcctgggc caactggcgc
7321 gggatcattc cgcagctcgc agacgcgccg ggtgatcgcc ccggacatgc tcggcttcgg
7381 ctacagcgaa cgtccggccg atggcaagta cagccaggcg cgctgggtcg agcatgccat
7441 cggcgtgctc gacgccctcg gcatccagca gggcgacatc gtcggcaact cgttcggcgg
7501 cgggctggca ctggcactgg ccatccgtca ccccgagcgt gtgcgccggc tggtgctgat
7561 gggcagcgtc ggtgtgtctt tccccatcac cgcaggactg gaaacagcct ggggctacac
7621 gccgtcgctg gccaacatgc gcaggctgct cgatctgttc gcccacgacc gcaccctggt
7681 caacgacgag ctggccgagc tgcgctacca ggccagcatc cgccccggct ttcaggagtc
7741 gttcgccgcg atgttcccgc cgccacggca gaacggagtc gacgatctgg ccagcaacga
7801 gaccgatatc cgcgccctgc ccaacgaaac cctggtcatc cacggccgcg aggatcggat
7861 catcccgctg caggcttcgc tgaccctcgc gcagtggatt cccaacgccc agctacacgt
7921 gttcggccag tgcggccact ggacccagat cgaacacgcc gagcgtttcg cccgcttggt
7981 cgagaatttc ctcgccgagg ccgacgccct ccattcctga gagagaccga tatggacaag
8041 acattgatca acgaactcgg cgacgagctc taccaggcga tggtccagcg cgagaccgtc
8101 acgccgctga ccagccgcgg cttcgacatc agcgtcgagg acgcctacca catttccctg
8161 cgcatgctgg aacggcgcct ggccgccggc gagcgggtga tcggcaagaa gatcggcgtc
8221 accagcaagg ccgtgcagaa catgctcggc gtgcaccagc cggacttcgg ctacctcacc
8281 gatgccatgg tctacaacag cggcgaagcc atgccgatca gcgagaagct gatccagccg
8341 cgcgccgagg gcgagatcgc cttcatcctc aagaaggacc tgatggggcc gggcgtgacc
8401 aacgccgacg tgctggctgc caccgaatgc gtgatcccct gcttcgaagt ggtcgattcg
8461 cgcatccagg actggaagat caagatccag gacaccgtgg cggacaacgc ctcctgcggg
8521 ctgttcgtgc tcggcgacca ggccgtctca ccgcgccagg tcgatctggt cacctgcggc
8581 atgctggtcg agaagaacgg ccagctgctc tccaccggcg ctggagcggc tgcgctcggc
8641 tcgccggtca attgcgtcgc ctggttggcc aacaccctcg gccacttcgg catcgcctga
8701 aggccggcga agtgatcctg tccggctcgc tggttccgct ggaaccggtc aaggccggtg
8761 atttcatgcg cgtcgagatc ggcggcatcg gcagcgcctc cgtgcgcttc atctgatcga
8821 ggacagcctg atgaacaaga aactgaaagt agcgatcata ggcccaggca acatcggcac
8881 agacctgatg atcaaggtga tgcgcaacgc acagtacttg gaaatggggg ccatggtggg
8941 tatcgacccg gcctccgatg gcttggcccg tgctcagcgc atgggcgtgg cgaccaccca
9001 tgaaggcgtc gaagggtgat aacgcctgcc ggaattc
//