GenBank-Updates@genbank.bio.net (05/15/91)
LOCUS R6KECOP 2476 bp ds-DNA BCT 15-MAY-1991
DEFINITION Plasmid R6K (from E.coli) p1 protein and gamma and beta origins of
replication.
ACCESSION M65025
KEYWORDS origin of replication; p1 protein.
SOURCE Plasmid R6K (from E.coli) DNA.
ORGANISM Plasmid R6K
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 2476)
AUTHORS Kelley,W.L.
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
misc_feature 1..442
/note="replication enhancer cis-region"
rep_origin 1..442
/note="replication origin gamma region"
promoter 443..622
/note="promoter autoregulatory region"
CDS 623..1540
/product="protein p1"
/gene="p1"
/codon_start=623
rep_origin 1450..2476
/note="replication origin beta region"
BASE COUNT 751 a 424 c 515 g 786 t
ORIGIN
1 gatcgctagt ttgttttgac tccatccatt agggcttcta aaacgccttc taaggccatg
61 tcagccgtta agtgttcctg tgtcactgaa aattgctttg agaggctcta agggcttctc
121 agtgcgttac atccctggct tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc
181 ttatatattc ttttttttct tataaaactt aaaaccttag aggctattta agttgctgat
241 ttatattaat tttattgttc aaacatgaga gcttagtacg tgaaacatga gagcttagta
301 cgttagccat gagagcttag tacgttagcc atgagggttt agttcgttaa acatgagagc
361 ttagtacgtt aaacatgaga gcttagtacg tgaaacatga gagcttagta cgtactatca
421 acaggttgaa ctgctgatct tcagatctag cttaaaacag gtggcttttt aatcatcttt
481 gccaagcatg gcgcgggttt ggggtaatat agcgactcat aaaagcgtta aacatgagtg
541 gatagtacgt tgctaaaaca tgagataaaa attgactctc atgttattgg cgttaagata
601 tacagaatga tgaggttttt ttatgagact caaggtcatg atggacgtga acaaaaaaac
661 gaaaattcgc caccgaaacg agctaaatca caccctggct caacttcctt tgcccgcaaa
721 gcgagtgatg tatatggcgc ttgctcccat tgatagcaaa gaacctcttg aacgagggcg
781 agttttcaaa attagggctg aagaccttgc agcgctcgcc aaaatcaccc catcgcttgc
841 ttatcgacaa ttaaaagagg gtggtaaatt acttggtgcc agcaaaattt cgctaagagg
901 ggatgatatc attgctttag ctaaagagct taacctgccc tttactgcta aaaactcccc
961 tgaagagtta gatcttaaca ttattgagtg gatagcttat tcaaatgatg aaggatactt
1021 gtctttaaaa ttcaccagaa ccatagaacc atatatctct agccttattg ggaaaaaaaa
1081 taaattcaca acgcaattgt taacggcaag cttacgctta agtagccagt attcatcttc
1141 tctttatcaa cttatcagga agcattactc taattttaag aagaaaaatt attttattat
1201 ttccgttgat gagttaaagg aagagttaac agcttatact tttgataaag atggaaatat
1261 tgagtacaaa taccctgact ttcctatttt taaaagggat gtgttaaata aagccattgc
1321 tgaaattaaa aagaaaacag aaatatcgtt tgttggcttc actgttcatg aaaaagaagg
1381 aagaaaaatt agtaagctga agttcgaatt tgtcgttgat gaagatgaat tttctggcga
1441 taaagatgat gaagcttttt ttatgaattt atctgaagct gatgcagctt ttctcaaggt
1501 atttgatgaa accgtacctc ccaaaaaagc taaggggtga tatatggcta aaatttacga
1561 tttccctcaa ggagccgaac gccgcaggat gcaccgcaaa atccagtgga acaacgctgt
1621 aaaattatct aaaaatggct ggagtaagcc agaggttaaa cgctggtctt ttttagcatt
1681 catctcaact ggctggtatt actttcgcct ttcggtagca gtcattttcc atatcattac
1741 tatttgtggt ttagctgtgc tcgcggcgtt aagcaatacg atattctgga ttggtggcgc
1801 gatatgtctt gtaacctggt atacaaatga ccatcaaatt tggagtacta acaatcttac
1861 tatccctatt gttttcggac tttgggtgtt aagtttagta gctgcaccac tcatagattt
1921 tttcagtcaa aaattgccct tttatcgtct tcttgtgcct gatgcgaagc gtgaggaagt
1981 gggcgaagat gattcttaaa gccctgccct gtacggcttt aacgccttct cgcggtagat
2041 ctatggatgt tgagaatgta gtatggttat actgcgatgc aggatagggc aaacgccgta
2101 aaatgacgtc tttgacgtta tttttcagcg cttgcccggt gacctacagt cggtgcttgt
2161 tgggagattt tatgaagttt actagtaaag gattttatca gtgataaata tgcaaaggct
2221 attaacattt taaatgataa ccttaaagaa aactactatg ttttttatgg tgtaaggtta
2281 agtgaaattc tttttcctgc aagtgattat ggtacagatg atttttttaa ggagtttgag
2341 gaaataaaca acgttacctt gcctttagtt gtttttgaaa taaatgaacg tgaacctgtg
2401 attgtaattg gttttgatga aataaatcct gcgattctta tagagaaatc cggtataaag
2461 gttttagtaa tcggac
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