GenBank-Updates@genbank.bio.net (01/16/91)
LOCUS ECAPNLA 2172 bp ds-DNA BCT 16-JAN-1991
DEFINITION Erwinia carotovora pectin lyase (pnlA) gene, complete cds.
ACCESSION M59909
KEYWORDS pectin lyase.
SOURCE Erwinia carotovora (Ecc71) DNA.
ORGANISM Erwinia carotovora
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2172)
AUTHORS Chatterjee,A., McEvoy,J.L., Chambost,J.P., Blasco,F. and
Chatterjee,A.K.
TITLE Nucleotide sequence and molecular characterization of pnlA, the
structural gene for damage-inducible pectin lyase of Erwinia
carotovora subsp. carotovora strain 71
JOURNAL Unpublished (1990)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS 601..1473
/EC_number="4.2.2.1.0"
/gene="pnlA"
/evidence=EXPERIMENTAL
/codon_start=601
/product="pectin lyase"
BASE COUNT 615 a 431 c 498 g 628 t
ORIGIN
1 tactgcaaaa aaacggatta attactctaa agcctgacac gggcttattg ccgacggtgc
61 tggatatcac gacgaatcca cacaacctgc gtattatgga agtggaaggg cacagttgcc
121 gcgtttgtta gacgatccac aagtgacggt agccattatt agtacgacct atattcagca
181 aacgggctta acgccaacga aagacggtat ttttattgag gataaagaat caccgtatgt
241 gaatatcatt gtgacgcgtg aaaataataa agaggcggaa aacgtcaaaa aatttattca
301 ggcctatcag tctgatgaag ttgaacaggc tgcgaaccgt atttttaatg gcggcgcggt
361 aaaaggctgg taatgataat aatgtgataa tcgtagctag cctgccattt tactaaaaga
421 tggcagcgta ttaattgggt attgaattat tcgcaaggtt gtttttttat taaactcgat
481 taataagcgt aatgaaatcc tttatataca atttttaatt gtcggaggcg tgttatttgt
541 ttttgattaa ataatacgtt ggaagacatt attattcact cattgtaaaa aggaaaattt
601 atggcttatc caacaacaaa tcttactggg attattggtt ttgcaaaagc ggcaaatgta
661 actggaggca cgggaggtaa agtcgttacg gtaaattctt tggccgattt taaatcagca
721 gtgagcggtt ccgcaaaaac tattgtggtg ctgggattat cgctgaaagc ctcggcattg
781 accaaggtgg tatttggcag caataaaacc atcgttggtt ctttcggtgg gtacgctaat
841 gtactgacca atattcacct gcgcgctgag agcaattcat ctaacgtcat tttccagaat
901 ctggttttta aacatgatgt tgccatcaaa gataatgacg atatccagct gtatttgaac
961 tacggcaagg gatattgggt ggatcactgt tcatggcctg ggcatacgtg gagcgataac
1021 gatggtagcc tcgataaact gatttatatc ggcgagaagg cggattacat cacgatcagt
1081 aactgcttat tctcaaacca taaatatggt tgcatcttcg gccatccggc agatgacaat
1141 aacagcgctt acaatggcta tccgcgtctg actatttgtc ataactatta cgaaaatatt
1201 caggtgcgtg cgcccggcct gatgcgctat ggttatttcc acgtcttcaa ccaacctacg
1261 tcaataaatt caacctggcc tttacagttg cgcaggaatg ccaatctaat ttctgagcgt
1321 aacgtatttg gtaccggcgc ggaaaataaa gggatggttg atgataaggg caatggttca
1381 accttacgga taatggcagt tcaccggctg cggtggcgag caaatcgcct gcggcgaaat
1441 ggacggcgtc atctaactat tcatacagtt tgatgaccac gtcagcggcg aaatcctggg
1501 tggtgtctaa tgcgggagcg caaaatagcg ctctcaaatt cccatcgtaa tcgttgaacg
1561 tatattgaat gacgttccag tatgcgcagt attttattgt gaagtgaaat gcattaaata
1621 ctgagtctat cacactatgc cacttggcga atttgttaat gccgagtggc atagttattg
1681 gttcatgttc aacgcaatat aataggttcg ttcaaaaaat taagcggcaa tattatttct
1741 cgtattgtga gtaaaatacc actctactgt tttcttaatt aactcttcgg ttgcatcgac
1801 atagtgttgc ggtgatgttc tgacttcctg ctccagaatc agaggaagct cggtacaacc
1861 cagaataatt ttctcaacgc cagcctgcaa caggcgatct tttaccggtg acaacaggct
1921 ataggcgccg gcaatatcac cggatttata agcatagatg ctttccatta cctggtgttg
1981 gtctgcatca tccggcgtgt agcaatcaat attatcggta atcaggttgt cctgataaat
2041 tctcgctttg accgtcgctg tcgtcgctaa taggccgacg cgtgtggtgt tggcatgctt
2101 aatcgcctga caggtcacat caataatgct gatcatttcc gcacggcact gctgttttaa
2161 gtcattaaac ca
//GenBank-Updates@genbank.bio.net (03/29/91)
LOCUS ECAPNLA 2172 bp ds-DNA BCT 29-MAR-1991
DEFINITION Erwinia carotovora pectin lyase (pnlA) gene, complete cds.
ACCESSION M59909
KEYWORDS pectin lyase.
SOURCE Erwinia carotovora (Ecc71) DNA.
ORGANISM Erwinia carotovora
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2172)
AUTHORS Chatterjee,A., McEvoy,J.L., Chambost,J.P., Blasco,F. and
Chatterjee,A.K.
TITLE Nucleotide sequence and molecular characterization of pnlA, the
structural gene for damage-inducible Pectin Lyase of Erwinia
carotovora subsp. carotovora 71
JOURNAL J. Bacteriol. 173, 1765-1769 (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS 601..1473
/EC_number="4.2.2.1.0"
/product="pectin lyase"
/gene="pnlA"
/evidence=EXPERIMENTAL
/codon_start=601
BASE COUNT 615 a 431 c 498 g 628 t
ORIGIN
1 tactgcaaaa aaacggatta attactctaa agcctgacac gggcttattg ccgacggtgc
61 tggatatcac gacgaatcca cacaacctgc gtattatgga agtggaaggg cacagttgcc
121 gcgtttgtta gacgatccac aagtgacggt agccattatt agtacgacct atattcagca
181 aacgggctta acgccaacga aagacggtat ttttattgag gataaagaat caccgtatgt
241 gaatatcatt gtgacgcgtg aaaataataa agaggcggaa aacgtcaaaa aatttattca
301 ggcctatcag tctgatgaag ttgaacaggc tgcgaaccgt atttttaatg gcggcgcggt
361 aaaaggctgg taatgataat aatgtgataa tcgtagctag cctgccattt tactaaaaga
421 tggcagcgta ttaattgggt attgaattat tcgcaaggtt gtttttttat taaactcgat
481 taataagcgt aatgaaatcc tttatataca atttttaatt gtcggaggcg tgttatttgt
541 ttttgattaa ataatacgtt ggaagacatt attattcact cattgtaaaa aggaaaattt
601 atggcttatc caacaacaaa tcttactggg attattggtt ttgcaaaagc ggcaaatgta
661 actggaggca cgggaggtaa agtcgttacg gtaaattctt tggccgattt taaatcagca
721 gtgagcggtt ccgcaaaaac tattgtggtg ctgggattat cgctgaaagc ctcggcattg
781 accaaggtgg tatttggcag caataaaacc atcgttggtt ctttcggtgg gtacgctaat
841 gtactgacca atattcacct gcgcgctgag agcaattcat ctaacgtcat tttccagaat
901 ctggttttta aacatgatgt tgccatcaaa gataatgacg atatccagct gtatttgaac
961 tacggcaagg gatattgggt ggatcactgt tcatggcctg ggcatacgtg gagcgataac
1021 gatggtagcc tcgataaact gatttatatc ggcgagaagg cggattacat cacgatcagt
1081 aactgcttat tctcaaacca taaatatggt tgcatcttcg gccatccggc agatgacaat
1141 aacagcgctt acaatggcta tccgcgtctg actatttgtc ataactatta cgaaaatatt
1201 caggtgcgtg cgcccggcct gatgcgctat ggttatttcc acgtcttcaa ccaacctacg
1261 tcaataaatt caacctggcc tttacagttg cgcaggaatg ccaatctaat ttctgagcgt
1321 aacgtatttg gtaccggcgc ggaaaataaa gggatggttg atgataaggg caatggttca
1381 accttacgga taatggcagt tcaccggctg cggtggcgag caaatcgcct gcggcgaaat
1441 ggacggcgtc atctaactat tcatacagtt tgatgaccac gtcagcggcg aaatcctggg
1501 tggtgtctaa tgcgggagcg caaaatagcg ctctcaaatt cccatcgtaa tcgttgaacg
1561 tatattgaat gacgttccag tatgcgcagt attttattgt gaagtgaaat gcattaaata
1621 ctgagtctat cacactatgc cacttggcga atttgttaat gccgagtggc atagttattg
1681 gttcatgttc aacgcaatat aataggttcg ttcaaaaaat taagcggcaa tattatttct
1741 cgtattgtga gtaaaatacc actctactgt tttcttaatt aactcttcgg ttgcatcgac
1801 atagtgttgc ggtgatgttc tgacttcctg ctccagaatc agaggaagct cggtacaacc
1861 cagaataatt ttctcaacgc cagcctgcaa caggcgatct tttaccggtg acaacaggct
1921 ataggcgccg gcaatatcac cggatttata agcatagatg ctttccatta cctggtgttg
1981 gtctgcatca tccggcgtgt agcaatcaat attatcggta atcaggttgt cctgataaat
2041 tctcgctttg accgtcgctg tcgtcgctaa taggccgacg cgtgtggtgt tggcatgctt
2101 aatcgcctga caggtcacat caataatgct gatcatttcc gcacggcact gctgttttaa
2161 gtcattaaac ca
//GenBank-Updates@genbank.bio.net (05/15/91)
LOCUS ECAPNLB 1291 bp ds-DNA BCT 15-MAY-1991
DEFINITION Erwinia carotovora pectin lyase (pnl) gene, complete cds.
ACCESSION M65057
KEYWORDS pectin lyase; pnl gene.
SOURCE Erwinia carotovora (strain Er) DNA.
ORGANISM Erwinia carotovora
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1291)
AUTHORS Ohnishi,H., Nishida,T., Yoshida,A., Kamio,Y. and Izaki,K.
TITLE Nucleotide sequence of pnl gene from Erwinia carotovora Er
JOURNAL Biochem. Biophys. Res. Commun. 176, 321-327 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 290..1234
/product="pectin lyase"
/gene="pnl"
/codon_start=290
BASE COUNT 366 a 253 c 297 g 375 t
ORIGIN
1 taccccctat cagtctgatg aagttgaaca ggctgcgaac cgtattttta atggcggcgc
61 ggtaaaaggc tggtgatgat aatcgtagcg ctgccatttt actaaaagat ggcggcgtat
121 taattgggta ttgaattatt cgcaaggttg tttttttatt aaactcgatt aataagcgta
181 atgaaatcct ttctatacaa tttttaattg tcggaggcgt attatttagt ctcaattaaa
241 taatacgctg gaagacatta ttattcactc attgtaaaaa ggaaaactta tggcttatcc
301 aacaacaaat cttactgggc ttattggttt tgcaaaagcg gcaaaagtta ccggaggaac
361 gggcggtaaa gtcgttacgg taaattcttt ggccgatttt aaatcagcgg tgagcggttc
421 cgcaaaaact attgtggtgc tgggatcatc gctgaaaacg tcggccttga ccaaggtggt
481 atttggcagc aataaaacca tcgtcggttc tttcggtggc gctaatgtac tgaccaatat
541 tcacctgcgt gctgagagca attcatctaa cgtcattttc cagaatctgg tttttaaaca
601 tgatgttgcc atcaaagata atgacgatat ccagctgtat ttgaactacg gtaaagggta
661 ttgggtggat cactgttcat ggcctgggca tacgtggagc gataacgatg gtagcctcga
721 taaactgatt tatatcggcg agaaggcgga ttacatcacg atcagtaact gcttattctc
781 aaaccataaa tatggttgca ttttcggcca tccggctgac gataataaca gcgcttacaa
841 tggctatcca cgtttgacta tttgccataa ctattacgaa aatattcagg tgcgtgcgcc
901 cggcctgatg cgttatggct atttccacgt attcaataac tacgtcaata aattccagtt
961 ggcttttacc gtcgcgcaaa atgccaacgt tatttctgaa cgcaacgtat ttggcagcgg
1021 tgctgaaaag aaagggatgg ttgatgataa aggcaatggt tcaaccttta ccgataatgg
1081 cagttcgcca gcagcggtag cgagtaaatc gccagcggcg aaatggacgg catcatctaa
1141 ctattcatac agtttgatga caaccgcggc ggcccaatcc tgggttgttt cgaatgcagg
1201 ggcacaaaat agtgcgctga aattcccatc ataatcacgg aaggtttatt ttatgacgta
1261 cctgcatgct cacttttatt gataattgaa a
//