GenBank-Updates@genbank.bio.net (05/15/91)
LOCUS LACNISIN 3289 bp ds-DNA BCT 15-MAY-1991
DEFINITION Lactococcus lactis nisin gene, complete cds, ORF A, complete cds,
and ORF B, 5' end.
ACCESSION M65089
KEYWORDS antibiotic; nisin.
SOURCE Lactococcus lactis DNA.
ORGANISM Lactococcus lactis
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 3289)
AUTHORS Steen,M.T., Chung,Y.J. and Hansen,N.
TITLE Characterization of the nisin gene as part of a polycistronic
operon in the chromosome of Lactococcus lactis ATCC 11454
JOURNAL Appl. Environ. Microbiol. 57, 1181-1188 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 1..174
/gene="nicin"
/codon_start=1
repeat_unit 222..266
/rpt_type=inverted
CDS 282..2840
/label=ORF A
/codon_start=282
repeat_unit 3042..3093
/rpt_type=inverted
repeat_unit 3094..3106
/rpt_type=inverted
-35_signal 3143..3148
-10_signal 3165..3170
-35_signal 3185..3190
-10_signal 3210..3215
CDS 3269..>3289
/label=ORF B
/codon_start=3269
BASE COUNT 1263 a 383 c 590 g 1053 t
ORIGIN
1 atgagtacaa aagattttaa cttggatttg gtatctgttt cgaagaaaga ttcaggtgca
61 tcaccacgca ttacaagtat ttcgctatgt acacccggtt gtaaaacagg agctctgatg
121 ggttgtaaca tgaaaacagc aacttgtcat tgtagtattc acgtaagcaa ataaccaaat
181 caaaggatag tattttgtta gttcagacat ggatactatc ctatttttat aagttattta
241 gggttgctaa atagcttata aaaataaaga gaggaaaaaa catgataaaa agttcattta
301 aagctcaacc gtttttagta agaaatacaa ttttatctcc aaacgataaa cggagtttta
361 ctgaatatac tcaagtcatt gagactgtaa gtaaaaataa agtttttttg gaacagttac
421 tactagctaa tcctaaactc tatgatgtta tgcagaaata taatgctggt ctgttaaaga
481 agaaaagggt taaaaaatta tttgaatcta tttacaagta ttataagaga agttatttac
541 gatcaactcc atttggatta tttagtgaaa cttcaattgg tgttttttcg aaaagttcac
601 agtacaagtt aatgggaaag actacaaagg gtataagatt ggatactcag tggttgattc
661 gcctagttca taaaatggaa gtagatttct caaaaaagtt atcatttact agaaataatg
721 caaattataa gtttggagat cgagtttttc aagtttatac cataaatagt agtgagcttg
781 aagaagtaaa tattaaatat acgaatgttt atcaaattat ttctgaattt tgtgagaatg
841 actatcaaaa atatgaagat atttgtgaaa ctgtaacgct ttgctatgga gacgaatata
901 gagaactatc ggaacaatat cttggcagtc tgatagttaa tcattatttg atctctaatt
961 tacaaaaaga tttgttgtca gatttttctt ggaacacttt tttgactaaa gttgaagcaa
1021 tagatgaaga taaaaaatat ataattcctc tgaaaaaagt tcaaaagttt attcaagaat
1081 actcagaaat agaaattggt gaaggtattg agaaactgaa agaaatatat caggaaatgt
1141 cacaaattct tgagaatgat aattatattc aaattgattt aattagtgat agtgaaataa
1201 attttgatgt taaacaaaag caacaattag aacatttagc tgagttttta ggaaatacga
1261 caaaatctgt aagaagaaca tatttggatg actataagga taaatttatc gaaaaatatg
1321 gtgtagatca agaagtacaa ataacagaat tatttgattc tacatttggc ataggagctc
1381 catataatta taatcatcct cgaaatgact tttatgagtc cgaaccgagt actctatact
1441 attcagaaga ggagagagaa aagtacctca gcatgtatgt agaagccgtt aaaaatcata
1501 atgtaattaa tcttgacgac ttagagtctc attatcaaaa aatggactta gaaaagaaaa
1561 gtgaacttca agggttagaa ttatttttga atttggcaaa ggagtatgaa aaagatattt
1621 ttattttagg ggatatcgtt ggaaataata atttgggagg ggcatcaggt agattttctg
1681 cactctctcc ggagttaaca agttatcata gaacgatagt agattctgtc gaaagagaaa
1741 atgagaataa agaaattaca tcgtgtgaaa tagtatttct tccagaaaat atcagacatg
1801 ctaacgttat gcatacatca attatgagga ggaaagtact tccatttttt acaagtacaa
1861 gtcacaatga agttctgtta actaatatct atattggaat agacgaaaaa gaaaaatttt
1921 atgcacgaga catttcaact caagaggtat tgaaattcta cattacaagc atgtacaata
1981 aaacgttatt cagtaatgag ctaagatttc tttacgaaat ttcattagat gacaagtttg
2041 gtaatttacc ttgggaactt atttacagag actttgatta tattccacgt ttagtatttg
2101 acgaaatagt aatatctcct gctaaatgga aaatttgggg aagggatgta aatagtaaga
2161 tgacaataag agaacttatt caaagcaaag aaattcccaa agagttttat attgtcaatg
2221 gagataataa agtttattta tcacaggaaa acccattgga tatggaaatt ttagagtcgg
2281 cgataaagaa gagctcaaaa agaaaagatt ttatagagct acaagaatat tttgaagatg
2341 aaaatatcat aaataaagga gaaaagggga gagttgccga tgttgtagtg ccttttatta
2401 gaacgagagc attaggtaat gaagggagag catttataag agagaaaaga gtttcggttg
2461 aacggcgtga aaaattgccc tttaacgagt ggctttatct aaagttgtac atttctataa
2521 atcgtcaaaa tgaattttta ctgtcgtatc ttccagatat tcagaaaata gtagcaaacc
2581 tgggtggaaa tctattcttc ctaagatata ctgatcctaa accacatatt agattgcgta
2641 taaaatgttc agatttattt ttagcttacg gatctattct tgaaatctta aaaaggagtc
2701 ggaaaaatag gataatgtca acttttgata tttctattta tgatcaagaa gtagaaagat
2761 atggtggatt tgatacttta gagttatccg aagcaatatt tgtgccgatt ctaaaattat
2821 tccaaatttg cttacattga taaaagatac taataatgat tggaaagtcg atgatgtatc
2881 aatcttggtg aattatttat atctgaaatg cttctttcag aatgataaca aaaagattct
2941 taattttttg aatttagtta gtactaaaaa ggttaaagaa aatgtcaatg aaaagattga
3001 acattatctt aagcttctga aagttaataa tctaggtgac caaatttttt atgacaagaa
3061 ttttaaagaa ttaaagcatg ccataaaaaa tttatttttt aaaaatgata gctcaagata
3121 cttcagaaag tttattcaat tattgacagt atcattcatg tccataataa ccgactaatt
3181 ggtattgaac gagataaaga gaaattaatt tattacacac ttcaaaggtt gtttgtttcg
3241 gaagaataca tgaaatgagg actaatagat ggatgaagtg aaagaattc
//