GenBank-Updates@genbank.bio.net (05/16/91)
LOCUS TOBEXT 3994 bp ds-DNA PLN 16-MAY-1991
DEFINITION N.plumbaginifolia extensin (ext) gene, complete cds.
ACCESSION M34371
KEYWORDS cell wall protein; extensin.
SOURCE N.plumbaginifolia leaf DNA.
ORGANISM Nicotiana plumbaginifolia
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Asteridae; Solanales; Solanaceae.
REFERENCE 1 (bases 1 to 3994)
AUTHORS De Loose,M., Tire,G.G., Villarroel,R., Genetello,C., Van
Montagu,M., Depicker,A. and Inze,D.
TITLE The extensin signal peptide allows secretion of a heterologous
protein from protoplasts
JOURNAL Gene 99, 95-100 (1991)
STANDARD unannotated staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by M.R.R.De Loose, 18-MAY-1990.
Author address: M.R.R.De Loose
Laboratorium voor Genetika, Wetenschappen,
K.L.Ledeganckstraat, Gent,
Oost-Vlaanderen, BELGIUM
FEATURES Location/Qualifiers
misc_signal 1532..1537
/note="TATA box"
mRNA 1560..3342
/note="extensin mRNA"
CDS 1590..2840
/note="extensin precursor"
/codon_start=1590
sig_peptide 1590..1652
/note="extensin signal peptide (could end at 1667)"
/codon_start=1590
mat_peptide 1653..2837
/note="extensin (could start at 1668)"
/codon_start=1653
repeat_region 1740..1824
/note="tandem 85 bp repeats"
repeat_region 1740..1758
/note="19 bp repeats"
misc_signal 3323..3329
/note="poly-A signal"
BASE COUNT 1264 a 992 c 550 g 1188 t
ORIGIN
1 cgcccgggga tcctcctgga tgccattttt cctttgcttt tggatacctt ttcgcaactt
61 tagtccattg cttgaacatg gttttttcta cctcttgtct gttcccatga tgataaacta
121 tgatagctaa cattttcagt aatattggat tgaattagat atactttgaa ttattgagct
181 acaaaattcg ttgaatattt tgccctatcc cccacccaaa agaaaataat tcataccttt
241 atttggtaat aaaagtgggt cacatgcaca gtttttctcg tcttctctag attaaactct
301 ttggaaatga cctactgaaa atactacaca taaaattccc cactcccctc caagttgggg
361 gagggtgggg gttggtgggt tttttttccc cttgaatcca ttcagccggc ttatgcctcc
421 cgaggtggtg ggaggtttaa tttgaaccct taccctatag ttaatatcga aatagataat
481 acaaaggagg ggaacataaa accaaaacct ccgacaaaac atcaaagtgt aggagttcat
541 gatatacgct tgattgaaag tgcacaatgg tctttgcata ctatcaaagt atcaattggt
601 tcttgaatta tatctcttaa tataatgtat tgtgtttaat ttctctacta tcccctattt
661 tataagacta aaagatcctg acatgcttct tgaacacatg tgaaggttag ttaactggtc
721 agaagtacac aagaattaat ttgtacacct atgtttctca ccagaatgcc cataaattac
781 atcaattctt caacatcaag agatcgaaaa acttaacaat tgttctaata gttgaactga
841 gtcgtccgat ccatatccga ttcttcacta gaagcattaa tcatatagag caatttaact
901 taatttacta tattggtaat catttacata gttaagttat aaaaccttgg agcgacacaa
961 ggatttacac taatcatgat tgaatattaa ctttactcaa tttatcaatg tctgatacaa
1021 gtgaattaat ttctctcttt gtgatttcgg tagaaaatta atgtcaagtt tcaaagtttt
1081 tctttttgaa attagtcata catgtgaata gaacattaat ttaagttaaa gaatcatata
1141 aaaaagttag ccgctttgat ttctgtaact gaaatcgtgc agagatgagg catccacact
1201 tgtttttcaa agcttcagta ctatttaaaa acaaagacag taaaaaggag actttttctt
1261 tgacattatt gcatcagaaa tagtatagct gccataatag tttattcctt tgcttagctt
1321 gcagcctcta tcaaacaaaa aaatacacca gctcaagtca atttgagccg acaacatgac
1381 aaaaccaaat caaatatgca tttttttttt tttttttttt tttttttttt tttttttttt
1441 tttacacttt ggtaggtgtt aagtaatcta gtgagacttt taccttcatt tatgaaaatc
1501 ttgaaaaggg taattgtcta attgaaagct atataaaggg gtcgaagtga agcttaagag
1561 gacaacaact tttctcattt gtttcaaaga tgggaaaaat ggcttctcta tttgccacat
1621 ttttagtggt tttagtgtca cttagcttag cttctgaaag ctcagcaaat tatcaatact
1681 catctccacc accacctaag aagccatacc acccttcacc aacaccatat tatcctgcac
1741 cagtttataa atctccacca ccaccaattc cggtttataa gtcaccacca ccacccaaga
1801 agccatacta ccctccacac accccagttt acaaatctcc accaccacca actccagttt
1861 acaagtcgcc accaccaccc aagaagccac actaccctcc acacacaccc gtttacaagt
1921 ctccaccacc acctactcca gtttacaagt caccaccatc acccaagaag ccacactatc
1981 ctccacacac tccggtttac aagtcgccac caccaccaac tcccgtttac aagtcgccac
2041 caccacccaa gaagccacac tatccaccac acaccccggt ttacaagtcg ccaccaccac
2101 caactcccgt ttacaagtcg ccaccaccac caaagaagcc acactatcct ccacacacac
2161 cagtttacaa gtcaccacca ccacccaaga agccatacta tccaccacac accccagttt
2221 acaagtcgcc accaccacca actccagttt acaagtcacc accaccacct aagaagccat
2281 accacccttc accaacaccg tatcatcctt caccagttta caagtcccct ccaccaccaa
2341 ctccagttta caagtcacca ccaccaccta agaagccata ccacccttca ccaacaccgt
2401 atcatccatc accagtttac aagtcccctc caccaccaac tccagtttac aagtcaccac
2461 caccacctaa gaagccatac cacccttcac caacaccgta tcatccatca ccagtttaca
2521 agtcccctcc accaccaact ccagtttaca agtcaccacc accacctaag aagccatacc
2581 acccttcacc aacaccgtat catcctgcac cagtttacaa gtccccacca ccaccaactc
2641 cagtttacaa gtcaccaccg ccaccagtga agccatacca tccttcacca acaccatacc
2701 atcctgcacc agtttataag tctccaccac caccaactcc tgtttacaag tcacctccac
2761 caccaactcc agtctacaag tctccaccac cacaccaccc ctatgtttac gcttctcctc
2821 ctcctcccta ccattactaa gaagtgacat cacaaagctg aaggtaatta aggcaaatta
2881 attaaaacat tttcaaaatg atatgtcagg aatgcaaaga aaattgcata actttgttta
2941 tgcaaaacat taatataata taaagaactg cgctctctgc ttattaacta tttttttagc
3001 ttcatttatt agttactagc tgttcagaat tttcagtatc ttttgatatt acaaagaacc
3061 taatcagaca atgtatattc ttatgcagga aaagcagaat gttgagctaa aagaaaggct
3121 ttttccattt tcgagaggca acgagaagaa aagaagaaga aaaaatagag taaataataa
3181 gccccacggg aggcgaagtt cttttgtagc ttcatgttgt ctaagctatt gatattgttt
3241 gtaccctata ttttatttct ctgtctttgt gtatgttttg ttcaatttcg atctccttgc
3301 aaaatgcaga gattatgagc tgaataaagt aagttaatat tattatacgt gttaattttc
3361 tactcctctc tctagccagc cttttgtttt ctctttttct tatttcattt tctttatatc
3421 aaaccatttt aggagagggc caaggagtga tccagtaaaa cacgaagatt agaagaaact
3481 tcccttcttt ttttttatct gaaaaaaatt taaagtcgag atttatctct ttttgtaatg
3541 gaatcatttc cacagttatg actattttca aattgatatc gccagaccta acaaattaaa
3601 ttactccttc cgctcatctg gccgtacact tctgctccat ctggccgtac agttctgctc
3661 catctggcag tgtacggaga atggataact taatattcag tatgaatttg gacataaaat
3721 cttctaagat tgctagtgaa ttttgtgagc gttattgtgt taaaacaaaa gacagaaaat
3781 tattagaaga attaaagaaa tatgaaaata gtatagaatc agaaatattt cgagtccaca
3841 agttttttgt gtgttcttaa ggaatgtttt aacccctcac acgttgccaa ggtaatggat
3901 taaatcctcc caagataaaa cggaataaac cttcctgcaa cagtggcaat acaaactgca
3961 ggtcgactct agggatcccc gggcgagcga attc
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