GenBank-Updates@genbank.bio.net (05/16/91)
LOCUS BACMLSERMD 1495 bp ds-DNA BCT 16-MAY-1991
DEFINITION B.licheniformis macrolide-lincosamide-streptogramin B (MLS)
resistance determinant (ermD) mRNA, complete cds.
ACCESSION M29832
KEYWORDS erythromycin-inducible protein;
macrolide-lincosamide-streptogramin B resistance.
SOURCE B.licheniformis, cDNA to mRNA, clone pBD90.
ORGANISM Bacillus licheniformis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 1495)
AUTHORS Gryczan,T., Israeli-Reches,M., Del Bue,M. and Dubnau,D.
TITLE DNA sequence and regulation of ermD, a
macrolide-lincosamide-streptogramin B resistance element from
Bacillus licheniformis
JOURNAL Mol. Gen. Genet. 194, 349-356 (1984)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
mRNA 76..>1327
/product="macrolide-lincosamide-streptogramin B resistance
methylase"
/gene="ermD"
CDS 109..153
/codon_start=109
CDS 430..1293
/product="macrolide-lincosamide-streptogramin B resistance
methylase"
/gene="ermD"
/codon_start=430
BASE COUNT 488 a 297 c 299 g 411 t
ORIGIN HpaI site.
1 aacccctgcg atattcgtaa ggagaagaaa attccagacg ttgacgtttt ccaaagaatg
61 ccctacaatg agatcgtaac tttaactttt caggaggatt attaaaaaat gacacactca
121 atgagacttc gtttcccaac tttgaaccag taattaaata cgttcaaagg ctctgtttgt
181 gtatgcagag taaacgggat cagtctgtcc ttttttaata atcttcaatt tgcatataaa
241 atttgtaatt tgtagaaagg cggatcgttc tgcctttctt ttttgtttaa tccttttttt
301 ctaacggctg agaaaggctt atttggttat ggaaataccg cggcttcatc cttaaaggat
361 ggctcttccc tttactctga atcacaggca gaccgcctgt gattttttat gatgagagga
421 agaggaaaca tgaagaaaaa aaatcataag tacagaggaa aaaagttaaa ccgcggggaa
481 tctccgaatt tttccggaca gcatttgatg cataataaaa aattaattga agaaattgtg
541 gatcgggcaa atattagcat agacgatacg gttttagagt taggagcggg aaaaggggct
601 ttgacaactg tgctaagtca aaaagccggt aaggtattgg cagtggaaaa cgattctaaa
661 ttcgttgata tactcacacg taaaacggca cagcattcaa atacgaaaat tattcatcaa
721 gatatcatga agattcattt accaaaagaa aagtttgtgg tggtctctaa tattccctat
781 gccatcacaa cccccatcat gaaaatgctt ttgaacaatc ctgcaagcgg atttcaaaaa
841 gggatcatcg taatggaaaa aggggctgct aaacgtttca catcaaaatt cattaaaaat
901 tcctatgttt tagcttggag aatgtggttt gatattggca ttgtcagaga aatatcgaaa
961 gagcattttt ctccccctcc aaaagtggac tcggcaatgg tcagaataac acgaaaaaaa
1021 gacgcgcctc tatcacataa acattatatt gcgtttcggg gacttgccga atacgcgcta
1081 aaggagccga atatccctct ctgtgttgct ttacgcggaa tttttacccc gcgtcaaatg
1141 aaacacttga gaaaaagtct aaaaatcaac aatgaaaaaa ccgttggaac gctcaccgaa
1201 aaccaatggg cggtcatttt taacacgatg actcagtatg taatgcatca caaatggcca
1261 agagcaaata agcgaaaacc cggagaaata taaagaaaaa gctgctgact tcccgtcagc
1321 agctttagct atttctggag ggattcagat gtccctgtca acattccttt tgtccgctta
1381 cagctttcca gttaaaaact gcgcctcgcc gaggccgaaa tcccaatcgg catctccatt
1441 ttctgtgatg gacaccatga catcttcagg tgaaatgccg cattccgctc aagct
//