GenBank-Updates@genbank.bio.net (05/16/91)
LOCUS FIBFSENDA 2075 bp ds-DNA BCT 16-MAY-1991
DEFINITION F.succinogenes ORF 1 and endo-1,4-beta-glucanase (FSendA) gene,
complete cds and 3' end.
ACCESSION M58520
KEYWORDS cellulase; endo-1,4-beta-glucanase.
SOURCE F.succinogenes (strain AR1) DNA, clone pHC79.
ORGANISM Fibrobacter succinogenes
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Sulfate- or sulfur-reducing dissimilatory bacteria.
REFERENCE 1 (bases 1 to 2075)
AUTHORS Cavicchioli,R., East,P.D. and Watson,K.
TITLE FSendA, a novel family E endoglucanase gene from Fibrobacter
succinogenes AR1
JOURNAL J. Bacteriol. 173, 3265-3268 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
-35_signal 314..319
/gene="FSendA"
-10_signal 336..341
/gene="FSendA"
mRNA 355..>2075
/gene="FSendA"
/evidence=EXPERIMENTAL
RBS 371..375
/gene="FSendA"
CDS 381..761
/gene="FSendA"
/label=ORF 1
/codon_start=381
-35_signal 613..618
/note="internal promoter"
CDS 626..1741
/EC_number="3.2.1.4"
/product="endo-1,4-beta-glucanase"
/gene="FSendA"
/codon_start=626
/partial
-10_signal 636..641
/note="internal promoter"
mRNA 653..>2075
/note="internal"
/evidence=EXPERIMENTAL
BASE COUNT 510 a 495 c 564 g 506 t
ORIGIN
1 gaattccaag aattttgccc gagaagccga aaccttcgaa cgcgtcaaat gctttggctc
61 ggccgttatc gagggctgtt ttaccgcctg tgggcgcctg gactgcttcg tgatgaccat
121 gagctacccc tgggatattg cggctattgc ccttctggta gaagaagcag gcggaaaatc
181 gacccatatc gatggcacgc ccatgcagtt tgtggacgcc gaacaagtca tttttagtaa
241 cggaatttta cataatacat tgatttcgac gcttcaatag agtcgtacca tcccagtgta
301 aatattcttt ttaatgaaaa tttgccaaaa taagctatat tggcattggg tttgcggtgt
361 ggtcattttt ggagaaagtc atgaattgta gaaaatacct tttgtcgggc cttgccgttt
421 ttggtttggc tgcgacgagt gctgttgcag cacttagcac cgacgactat gtggaagctg
481 cgtggatgac aacccgattc ttcggtgcgc agcgttctgg ccagggcccc aactggattt
541 tggatggcac gagcaacccg actagcttta ccaaggatag ttataacggt aaagacgtga
601 gcggtggttg gtttgactgc ggtgaccacg tgatgtatgg tcagtcccag ggttacgcct
661 cttatgtgct cgccttggct tatgccgaat ttaccgaagt ttctacgacc tttatactgg
721 tgactacacc gactacaagg aagccaacaa ctacaccatg aagagcggta agcccaacaa
781 ggtgcgtgac ctgctcgaag aactccgcta cgaagcagat ttctgggtaa aggctgccat
841 cgatggaaac aactttgtga cggttaaggg cgatggtaac gcagaccacc agaaatgggt
901 gactgctggt gccatgagca agctcggctc gggcgaaggt ggtgaaccgc gttgcattac
961 gggtaacgca aacgatggct ttacttcggg ccttgccgcc gctatgcttg ccgtgatggc
1021 tcgtgttgac cctgatacgg caaatcaggc caagtacctg aaggctgcaa agactgccta
1081 ttcttacgcc aagtctcaca agggcgttac gaactctcag ggcttctatg aatctagctg
1141 gtgggatggt cgttgggaag acggcccgtt tcttgctgaa cttgaacttt accgcactac
1201 cggtgaaaat tcttacaaga cagccgctat tgaccgttat gataacttga agttcagcct
1261 gggcgaaggc acgcacttta tgtacagtaa cgtggtgccg ctgtctgccg tgatggccga
1321 agccgtgttc gaagaaactc cgcatggcat gcgtaaggaa gccatcggcg tgttggactt
1381 gatctacgaa gaaaaggcca aggacaagat tttccagaat cccaatggca tgggttcggg
1441 caagttcccg gtacgtgttc cgtcgggtgg tgctttcctg tacgcattgt ctgacaagtt
1501 caataacacg aacgaacaca tggaaatgat cgaaaagaac gtgagctact tgcttggcga
1561 taacggcagc aagaagtctt acgtggttgg tttctccaag aacggcgcga acgctccgtc
1621 tagaccgcac catcgtggct actatgctaa cgaaaaacgc tggcgtcgaa gtcggcggtg
1681 ctccgaatcc tcccgaaaag aacaagctct tgggcggtat gattgctggc gactttacta
1741 gcggaaacca tgacggcaat acgtccaact ggcagacgaa cgaagtttgc gttgacttga
1801 acgctccgct cgttggcgct ctcggctata tcttgagcaa gaaggctccg aagaccgacg
1861 aagatctcgg catcaagtct attgtcaaga aggacacgac gaagaaggat acggttgtca
1921 aggatacgac gaagaaggat accatcgaag ctatcgttcc gcgtcttgcc ttggccaaga
1981 gtttcaacct gacttctaac ggttccttgg tgagtgtttc ccaggttgca cgcaagccct
2041 tcaaggtgca ggtgttcgac ctcacaggta agctt
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