GenBank-Updates@genbank.bio.net (03/13/91)
LOCUS VIBTDHI 570 bp ds-DNA BCT 13-MAR-1991
DEFINITION V.parahaemolyticus thermostable direct hemolysin (tdh/I) gene,
complete cds.
ACCESSION D90238
KEYWORDS hemolysin; tdh/I gene; thermostable direct hemolysin.
SOURCE V.parahaemolyticus (subspecies KP-negative; strain TH012) DNA, from
patient 012, clone pNY1.
ORGANISM Vibrio parahaemolyticus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 570)
AUTHORS Honda,T., Abad-Lapuebla,M.A., Ni,Y., Yamamoto,K. and Miwatani,T.
TITLE Characterization of a new thermostable direct hemolysin produced by
a Kanagawa phenomenon-negative clinical isolate of Vibrio
parahaemolyticus
JOURNAL J. Gen. Microbiol. (1990) In press
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Koichiro Yamamoto
Research Institute for Microbial Diseases
Osaka University
3-1 Yamada-oka
Suita
Osaka 565
Japan
Phone: 06-875-1014
Email: GAH02133 (NIFTY-SERVE)
Fax: 06-876-2678
FEATURES Location/Qualifiers
CDS 1..570
/note="thermostable direct hemolysin precursor"
sig_peptide 1..72
/note="thermostable direct hemolysin signal peptide"
mat_peptide 73..567
/note="thermostable direct hemolysin"
BASE COUNT 184 a 95 c 106 g 185 t
ORIGIN
1 atgaagtacc gatattttgc aaaaaaatca tttttattta tatccatgtt ggcagcattc
61 aaaacatttg cctttgagct tccatctgtc ccttttcctg cccccggttc tgatgagata
121 ttgtttgttg ttcgagatgc aacttttaat accaatgcac cggtcaatgt aaaggtctct
181 gacttttgga caaaccgtaa tgtaaaaaga aaaccgtaca aagatgttta tggtcaatca
241 gtattcacaa cgtcaggtac taaatggctg acatcctaca tgactgtgaa cattaatgat
301 aaagactata caatggcagc ggtgtctggc tataagcgcg gtcattctgc tgtgttcgta
361 aaatcagatc aagtacaact tcaacattcc tataattctg tagctaactt tgttggtgaa
421 gatgaagatt ctattccaag taaaatgtat ttggatgaaa ctccagaata ttttgttaat
481 gtagaagcat atgaaagtgg tagtggtaat atattggtaa tgtgtatatc caacaaagaa
541 tcgttttttg aatgtaaaca tcaaaagtaa
//GenBank-Updates@genbank.bio.net (04/12/91)
LOCUS VIBTDHI 1091 bp ds-DNA BCT 12-APR-1991
DEFINITION V.parahaemolyticus thermostable direct hemolysin (tdh/I) gene,
complete cds.
ACCESSION D90238
KEYWORDS hemolysin; tdh/I gene; thermostable direct hemolysin.
SOURCE V.parahaemolyticus (subspecies KP-negative; strain TH012) DNA, from
patient 012, clone pNY1.
ORGANISM Vibrio parahaemolyticus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 1091)
AUTHORS Honda,T., Abad-Lapuebla,M.A., Ni,Y., Yamamoto,K. and Miwatani,T.
TITLE Characterization of a new thermostable direct haemolysin produced
by a Kanagawa-phenomenon-negative clinical isolate of Vibrio
parahaemolyticus
JOURNAL J. Gen. Microbiol. 137, 253-259 (1991)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Koichiro Yamamoto
Research Institute for Microbial Diseases
Osaka University
3-1 Yamada-oka
Suita
Osaka 565
Japan
Phone: 06-875-1014
Email: GAH02133 (NIFTY-SERVE)
Fax: 06-876-2678
FEATURES Location/Qualifiers
CDS 227..796
/product="thermostable direct hemolysin"
/codon_start=227
sig_peptide 227..298
/product="thermostable direct hemolysin"
/codon_start=227
mat_peptide 299..793
/product="thermostable direct hemolysin"
/codon_start=299
BASE COUNT 352 a 170 c 191 g 378 t
ORIGIN
1 aagcttattt cagctatcta ccaagcgata aggcaggcca tgttaccgct tgagatcaca
61 gatgacgttg attattacgc aacaaaaaag cctcatagag ttgtaagcac tatcaaagat
121 ttcaagaagt attttttcat aattattcag tttgcttttt tgggtttttt agctttcatg
181 aagcctgcca ttctggcaaa gttattaatc aattcagagg ttttttatga agtaccgata
241 ttttgcaaaa aaatcatttt tatttatatc catgttggca gcattcaaaa catttgcctt
301 tgagcttcca tctgtccctt ttcctgcccc cggttctgat gagatattgt ttgttgttcg
361 agatgcaact tttaatacca atgcaccggt caatgtaaag gtctctgact tttggacaaa
421 ccgtaatgta aaaagaaaac cgtacaaaga tgtttatggt caatcagtat tcacaacgtc
481 aggtactaaa tggctgacat cctacatgac tgtgaacatt aatgataaag actatacaat
541 ggcagcggtg tctggctata agcgcggtca ttctgctgtg ttcgtaaaat cagatcaagt
601 acaacttcaa cattcctata attctgtagc taactttgtt ggtgaagatg aagattctat
661 tccaagtaaa atgtatttgg atgaaactcc agaatatttt gttaatgtag aagcatatga
721 aagtggtagt ggtaatatat tggtaatgtg tatatccaac aaagaatcgt tttttgaatg
781 tgaacatcaa aaataaaaag taaaagtaaa aagccacaga atattctgtg gctttgaaaa
841 tgattaataa taatttatct aatttatgca tgccaatgca aaactagatt gagtacgtgt
901 catattttta ttataaccaa caaccacatg ccagtttgtt gacaatatca aacaaaagag
961 aatcattatt aataatgttt ttagcttatg gttttttatg aattaaatta aatgtttttg
1021 cattgtgcta tgttgtttca cgctatttat ttggttacac tttagaggct ttgttgcgta
1081 atagtgaatt c
//GenBank-Updates@genbank.bio.net (05/16/91)
LOCUS VIBTDHI 1035 bp ds-DNA BCT 16-MAY-1991
DEFINITION V.parahaemolyticus thermostable direct hemolysin (tdh/I) gene,
complete cds.
ACCESSION D90238
KEYWORDS hemolysin; tdh/I gene; thermostable direct hemolysin.
SOURCE V.parahaemolyticus (subspecies KP-negative; strain TH012) DNA, from
patient 012, clone pNY1.
ORGANISM Vibrio parahaemolyticus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 1035)
AUTHORS Honda,T., Abad-Lapuebla,M.A., Ni,Y., Yamamoto,K. and Miwatani,T.
TITLE Characterization of a new thermostable direct haemolysin produced
by a Kanagawa-phenomenon-negative clinical isolate of Vibrio
parahaemolyticus
JOURNAL J. Gen. Microbiol. 137, 253-259 (1991)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Koichiro Yamamoto
Research Institute for Microbial Diseases
Osaka University
3-1 Yamada-oka
Suita
Osaka 565
Japan
Phone: 06-875-1014
Email: GAH02133 (NIFTY-SERVE)
Fax: 06-876-2678
FEATURES Location/Qualifiers
CDS 171..740
/product="thermostable direct hemolysin"
/codon_start=171
sig_peptide 171..242
/product="thermostable direct hemolysin"
/codon_start=171
mat_peptide 243..737
/product="thermostable direct hemolysin"
/codon_start=243
BASE COUNT 337 a 157 c 178 g 363 t
ORIGIN
1 aagcttcgtt aaatcgaatt acgcaacaaa aaagcctcat agagttgtaa gcactatcaa
61 agatttcaag aagtattttt tcataattat tcagtttgct tttttgggtt ttttagcttt
121 catgaagcct gccattctgg caaagttatt aatcaattca gaggtttttt atgaagtacc
181 gatattttgc aaaaaaatca tttttattta tatccatgtt ggcagcattc aaaacatttg
241 cctttgagct tccatctgtc ccttttcctg cccccggttc tgatgagata ttgtttgttg
301 ttcgagatgc aacttttaat accaatgcac cggtcaatgt aaaggtctct gacttttgga
361 caaaccgtaa tgtaaaaaga aaaccgtaca aagatgttta tggtcaatca gtattcacaa
421 cgtcaggtac taaatggctg acatcctaca tgactgtgaa cattaatgat aaagactata
481 caatggcagc ggtgtctggc tataagcgcg gtcattctgc tgtgttcgta aaatcagatc
541 aagtacaact tcaacattcc tataattctg tagctaactt tgttggtgaa gatgaagatt
601 ctattccaag taaaatgtat ttggatgaaa ctccagaata ttttgttaat gtagaagcat
661 atgaaagtgg tagtggtaat atattggtaa tgtgtatatc caacaaagaa tcgttttttg
721 aatgtgaaca tcaaaaataa aaagtaaaag taaaaagcca cagaatattc tgtggctttg
781 aaaatgatta ataataattt atctaattta tgcatgccaa tgcaaaacta gattgagtac
841 gtgtcatatt tttattataa ccaacaacca catgccagtt tgttgacaat atcaaacaaa
901 agagaatcat tattaataat gtttttagct tatggttttt tatgaattaa attaaatgtt
961 tttgcattgt gctatgttgt ttcacgctat ttatttggtt acactttaga ggctttgttg
1021 cgtaatagtg aattc
//