GenBank-Updates@genbank.bio.net (05/16/91)
LOCUS PSEAMNH 5215 bp ds-DNA BCT 16-MAY-1991
DEFINITION P.chlororaphis amidase and nitrile hydratase genes.
ACCESSION D90216 M65204
KEYWORDS amidase; nitrile hydratase.
SOURCE P.chlororaphis (strain B23) DNA.
ORGANISM Pseudomonas chlororaphis
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 5215)
AUTHORS Nishiyama,M., Horinouchi,S., Kobayashi,M., Nagasawa,T., Yamada,H.
and Beppu,T.
TITLE Cloning and characterization of genes responsible for metabolism of
nitrile compounds from Pseudomonas chlororaphis B23
JOURNAL J. Bacteriol. 173, 2465-2472 (1991)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Makoto Nishiyama
Department of Agricultural Chemistry
The University of Tokyo
1-1-1 Yayoi, Bunkyo-ku
Tokyo 113
Japan
Phone: 03-812-2111 x5126
Fax: 03-812-0544
FEATURES Location/Qualifiers
RBS 245..248
/product="amidase"
CDS 254..1774
/EC_number="3.5.1.4"
/product="amidase"
/codon_start=254
RBS 1857..1861
/product="nitrile hydratase alpha-subunit"
CDS 1869..2471
/EC_number="4.2.1.84"
/product="nitrile hydratase alpha-subunit"
/codon_start=1869
RBS 2502..2505
/product="nitrile hydratase beta-subunit"
CDS 2514..3176
/EC_number="4.2.1.84"
/product="nitrile hydratase beta-subunit"
/codon_start=2514
RBS 3160..3168
/standard_name="P47K"
CDS 3173..4432
/standard_name="P47K"
/codon_start=3173
RBS 4439..4443
/standard_name="ORF E"
CDS 4450..>5215
/standard_name="ORF E"
/codon_start=4450
BASE COUNT 1056 a 1626 c 1555 g 978 t
ORIGIN
1 ttcgaaccgt tgcgcgcagg ggccaatgcc tcgctttttt tattatcggc acgcgcagcg
61 atctgatctt tatcttctga gtgcagatgt cttttcaacc aaatgagtgt cctgctcaac
121 catgcaaata cggccatagc ggcctaattt tatctccatt gcgacccgga ctcagtgggt
181 cccacgggct cgaaacggtt gtcgaacaat cccgagccgg tgtgtcgatc atcgatcaaa
241 agacaggaaa atcatggcca ttactcgccc taccctcgac caggttttag acatccgaac
301 ccagttgcac atgcaactga cgcacgaaca ggcagcgtcc tacctggaac tgatgcaacc
361 gagtttcgac gcctacgacc tggtcgacga actggctgat ttcgttccgc caatacgcta
421 cgaccgcagt tcaggctatc gccatcggcc atcggccaag gaaaaccctc tgaacgcctg
481 gtactaccga acagaagtga atggtgcccg cgaaggcctg ctggcgggca aaaccgtcgc
541 gctcaaagat aatatctccc tggcaggcgt ccccatgatg aacggcgcag cgccgttgga
601 aggcttcgtc ccggggttcg atgccacggt ggtcacccgc ttgctcgatg cgggggcgac
661 cattctcggc aaagccacct gcgagcacta ctgcctttca ggaggcagcc acacctccga
721 tccagccccg gtgcacaacc cacatcgcca cggttatgcc tctggcggtt cctcatcagg
781 cagcgcggca ttggttgcgt ccggtgaggt ggacatcgcc gtgggcggcg atcaaggcgg
841 ctccattcgg atcccgtcgg ccttctgcgg tacctacggc atgaagccca cccacggcct
901 ggtgccctac accggcgtca tggcgattga agccacgatc gatcatgtcg gccccatcac
961 cggtaacgtg cgcgacaacg cgctgatgct gcaggcaatg gccggtgcag acggactcga
1021 cccgcgccag gcggcgcctc aggtcgatga ctattgcagt tacctggaaa aaggcgtgag
1081 cggactcaga atcggggtgt tgcaagaggg attcgcgctt gctaaccagg accctcgcgt
1141 ggcggacaaa gtgcgcgacg ccatcgcccg actcgaggcg ttgggcgctc atgtcgagcc
1201 ggtctccatt cccgagcaca acctggcagg gttgttgtgg caccccatcg gttgcgaagg
1261 cttgaccatg cagatgatgc atggcaacgg cgcaggcttt aactggaaag gactttacga
1321 tgtcggcctg ctggacaaac aagccagctg gcgcgacgac gcagaccaat tatccgcgtc
1381 gctcaagctc tgcatgttcg tcggccaata cggcctgtcg cgctacaacg gacgctacta
1441 cgccaaggcc cagaaccttg cacgctttgc ccggcaggga tacgacaaag cgctgcaaac
1501 ctatgacctg ctggtgatgc cgaccacgcc catcacggcc caaccccacc cgccagcgaa
1561 ctgctcgatc acggagtacg tggctcgcgc gttggaaatg atcggcaata ccgcgccaca
1621 ggacatcacc gggcatccgg ccatgtcgat tccgtgtggc ctgctggacg gcctgcccgt
1681 cgggctgatg ctggtcgcaa aacactacgc cgagggcacg atttaccaag cggcggcggc
1741 gtttgaagcc tcggtggact ggcgcacgct ctgagccttt tacaggcgcc gccccccctg
1801 aagaacgata agaaagaccg gcaagttgca atgacttttc aaccgcgttg actgatagga
1861 gttaccccat gagtacatct atttccacga ctgcgacacc ttcgacaccc ggcgagaggg
1921 catgggcctt gtttcaagtg ctcaagagca aggaactcat cccagagggc tatgtcgagc
1981 agctcactca attgatggcc catgactgga gcccggagaa cggcgctcgc gtggtcgcca
2041 aggcatgggt cgatccgcag ttccgggcgc tgctgctcaa ggacggaaca gccgcttgcg
2101 cgcagttcgg ctacaccggc ccacaaggcg aatacatcgt cgccctggaa gatacaccgg
2161 gggtgaagaa cgtcatcgtc tgcagcctgt gctcctgcac caactggccg gtcctcggcc
2221 tgccgcccga gtggtacaag ggctttgagt ttcgtgcgcg cctggtccgg gaggggcgca
2281 ccgtactgcg cgagctgggg acggagttgc cgagcgacac ggtcatcaaa gtctgggata
2341 ccagcgccga aagccgttac ctggtgttgc cgcaaaggcc tgaaggctct gagcacatga
2401 gtgaagaaca gcttcaacag ctggtgacca aagacgtgct gattggcgtc gccctgccac
2461 gcgttggctg agaaaaaaca actcatcatc gttcaacttg cggagttttc attatggatg
2521 gctttcacga tctcggcggt ttccaaggct ttggcaaagt gccgcacacc atcaacagcc
2581 tcagctacaa acaggttttc aagcaggact gggaacacct ggcctatagc ttgatgtttg
2641 tcggcgttga ccaattgaaa aagttcagcg tggacgaagt gcgtcatgcc gtcgaacgcc
2701 tggacgttcg ccagcatgtc ggcacccagt actacgaacg ctacatcatc gcgaccgcca
2761 cgctgctggt ggaaacgggc gttatcaccc aggcggagct cgatcaggca ttgggttccc
2821 acttcaagct ggcgaacccc gcccatgcga caggtcgccc ggcgatcacc ggcaggccgc
2881 ccttcgaagt gggcgatcgg gttgtggttc gagacgaata tgtggcgggg catatccgca
2941 tgccggccta cgtgcgcggt aaggaaggcg tggtcctgca ccgcacctca gagcagtggc
3001 ccttccccga cgccattggc cacggcgact tgagcgcagc ccatcagcct acctaccacg
3061 tcgagtttcg cgtgaaagat ctatggggtg acgcggcaga tgacggttac gtcgtggtcg
3121 atcttttcga aagctacttg gataaggccc ccggtgccca agcggtgaac gcatgattga
3181 aggcgcccag gcgggccgac tgccggtgac ggtcctttcc ggcttcctcg gcgccggcaa
3241 aaccaccctg ctcaacgcta tcctgcgaaa tcgccaagga ctgcgggtcg cggtcatcgt
3301 caacgacatg agcgaagtca acctcgatgc cgaatcggtg cagcgcgacg tctcgctgca
3361 ccgtggccgc gatgagttga tcgagatgag caatggctgc atttgctgca ccctgcgcgc
3421 cgacctgctg gaacagatca gtgacctggc acgtcagcag cgcttcgatt acctgcttat
3481 cgaatccacg gggatctccg aaccgatgcc ggtcgcggag accttcgcct tcctcgacac
3541 cgaaggcttc agcctcagcg agctggcacg ccttgataca ctggtgacgg tggtcgatgg
3601 cagccaattt caggcgctgc tggaatcgac ggataccgtt gcccgcgccg acaccgaggc
3661 gcacacgtcc acgcgtcacc tggcggatct tctgatcgaa caggtggagt acgccaacgt
3721 cattctggtc aacaagcggg acctgatcga cgagcccggc taccaggcag tgcatgcgat
3781 cctcgctggc ctcaacccga gcgcgcggat catgccgatg gctcacggga acgtcgcgct
3841 gtccagcctc ctcgacaccc atctgtttga tttacccagc ctcgcagcct cgccgggctg
3901 gatgcgtaaa atggaagcta ccgatacgcc ggcctcggag tcggatacct atggcgtgac
3961 atcctgggtc taccgggagc gcgcgccttt tcacccccag cgcttgctcg agtttctaca
4021 gaagccctgg cacaacggtc gcttgttgcg cagcaaaggt tacttctggc tcgccagccg
4081 ccacctggaa atcggcctgc tggcgcaaag cggcaagcag ttccagtggg attatgtcgg
4141 gcgctggtgg aacttcatcg agccatcgca atggccgcgg gacgaatatc ggttgcaggg
4201 catcatggcc aagtgggaca gcgttgtcgg cgactgccga caggagctgg tcttcatcgg
4261 ccaggggctc gacacccgcg tcttgcagcg cgaactcgac cattgtttgc tgagtgccca
4321 ggaaatagcc gccggcccac tggcctggca ggccctgccg gcggcgacgg cctttgacac
4381 cgaggcctta tcggcacgcc ccacgccccc catggcggtt caaccgactt gacctggtag
4441 aggtacacca tgctcttttt tagccgtacc acccgctggg tcttggcgcc gcttgccgac
4501 agactggacc agcctgacca ggcatgcacg ccgttgtcgt cgaacaaccc tttgttgcga
4561 cgcatcctga ccggcgtcga gcaattactg caagagcgca gcgccctgaa agaacaggca
4621 cgcgcgctga cacaggaagt cacccaactg gacgagcgcg tggcctgccg tgacgcgctt
4681 ttgcggcaat gggaggctcg ctgggccctg gtcagccacg gcgcaggtga actgttctgg
4741 gagctggagg tgaatggaac cgctgcaccg tcactggcat gcgcaatgag gtggacgggg
4801 tctgcgtcga tcctggctcc gggcattgat cagcttggcc attggaacga acagctccat
4861 ccggccgacc gtcaacgcca tttggatacc ctcgccaggc acctggccga tcgcagcggg
4921 cgtacgccgt ttgtgttgga cgtgcggata cagcccccat cgggcgacga ctatcgctgg
4981 tgcaggatca gcggtgattc acgccgcgat gcgcaaggcc tgcctgtggc gatgggtggc
5041 agcctgcggg acattcacca acaacacctg caagacgagg tgctggaact ggcggcgaca
5101 cgcttcgaca tctcccggga aatgctccat gacgggctct gggatatcga ggtcgtggcc
5161 ggcgacccgg cgaacccgaa aaataccatc tggtggtcat cgcaaatgcg tcgac
//