GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS CAJAPHA3 1426 bp ds-DNA BCT 17-MAY-1991
DEFINITION Plasmid pIP1433 (from Campylobacter coli) kanamycin resistance
(aphA) gene, complete cds.
ACCESSION M26832
KEYWORDS aphA gene; kanamycin resistance.
SOURCE Plasmid pIP1433 (from Campylobacter coli) DNA.
ORGANISM Plasmid pIP1433
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 1426)
AUTHORS Trieu-Cuot,P., Gerbaud,G., Lambert,T. and Courvalin,P.
TITLE In vivo transfer of genetic information between gram-positive and
gram-negative bacteria
JOURNAL EMBO J. 4, 3583-3587 (1985)
STANDARD full staff_entry
FEATURES Location/Qualifiers
-35_signal 161..166
/gene="aphA-3"
TATA_signal 184..189
/gene="aphA-3"
misc_feature 198..198
/note="transcription start site"
/gene="aphA-3"
RBS 581..590
/note="transcription start site"
/gene="aphA-3"
CDS 604..1398
/product="kanamycin resistance protein"
/gene="aphA-3"
/codon_start=604
BASE COUNT 473 a 235 c 349 g 369 t
ORIGIN
1 gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta tgaaaacgag
61 aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta ccaagacgaa
121 gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac tgataagata
181 atatataata tatctttact accaagacga taaatgcgtc ggaaaagtta aactgcgaaa
241 aaattggaac cggtacgctt atatagaaga tatcgccgta tgtaaggatt tcagggggca
301 aggcataggc agcgcgctta tcaatatatc tatagaatgg gcaaagcata aaaacttgca
361 tggactaatg cttgaaaccc aggacaataa ccttatagct tgtaaattct atcataattg
421 tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa acaactttga
481 aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat tggagttcgt
541 cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa
601 taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc
661 gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa
721 aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg
781 gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt
841 gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg
901 gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc
961 aggctctttc actccatcga catatcggat tgtccctata cgaatagctt agacagccgc
1021 ttagccgaat tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg
1081 gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag
1141 cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa
1201 gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat
1261 gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag
1321 ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta
1381 ctggatgaat tgttttagta cctagattta gatgtctaaa aagctt
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