GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS PSETCBCDEF 6960 bp ds-DNA BCT 17-MAY-1991
DEFINITION Pseudomonas sp. regulatory protein (tcbR), catechol 1,2-dioxygenase
II (tcbC), cycloisomerase II (tcbD), hydrolase II (tcbE), and
trans-dienelactoneisomerase (tcbF) genes, complete cds.
ACCESSION M57629
KEYWORDS catechol 1,2-dioxygenase II; cycloisomerase II; hydrolase II;
regulatory protein; tcbC gene; tcbD gene; tcbE gene; tcbF gene;
tcbR gene; trans-dienelactoneisomerase.
SOURCE Pseudomonas sp. (strain P51) DNA.
ORGANISM Pseudomonas sp.
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 6960)
AUTHORS van der Meer,J.R., Eggen,R.I., Zehnder,A.J. and de Vos,W.M.
TITLE Sequence analysis of the Pseudomonas sp. strain P51 tcb gene
cluster, which encodes metabolism of chlorinated catechols:
Evidence for specialization of catechol 1,2-deoxygenase for
chlorinated substrates
JOURNAL J. Bacteriol. 173, 2425-2434 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS complement(637..1521)
/product="regulatory protein"
/gene="tcbR"
/codon_start=1521
CDS 1672..2427
/EC_number="1.13.11.1"
/product="catechol 1,2-dioxygenase II"
/gene="tcbC"
/codon_start=1672
CDS 2424..3536
/EC_number="5.5.1.1"
/product="cycloisomerase II"
/gene="tcbD"
/codon_start=2424
CDS 3538..4548
/note="ORF"
/codon_start=3538
CDS 4570..5286
/product="hydrolase II"
/gene="tcbE"
/codon_start=4570
CDS 5283..6341
/product="trans-dienelactoneisomerase"
/gene="tcbF"
/codon_start=5283
BASE COUNT 1342 a 2155 c 2202 g 1261 t
ORIGIN
1 gagctcgcgc acgtccgagc ggatggccga tctgatggtt gacctgatga ttcgttatct
61 tccggccgaa tcaggtcgac gttgcttggg cgccgtgccg ttccatgtgc aaaaagcgga
121 agccttcatg cgcgagaact ttcgggaagc gattacgctg gccgaggttg cgtcagccgc
181 cggagtaact aagcgctgcc tttgtataaa tttcaggcga tttcgaaata cgagtcctat
241 ggcgtacctg cgcctccacc gattgacgca ggctagaaag atgctcgatc gacgcaagct
301 cgcgcttccg tcacggaggt ggctggagaa tgcgcactgc atcacctggg gcgtttctct
361 caagagtacc gactcgagtt tggggaacta ccctcgcaga ccatcgagcg agtcaatcga
421 gcccacctca agtgatccga taagcttgct tgacggtagc tacgaatgct tgccatcaga
481 actgcaacct ttctgactct tttgcatctg tgacggcggg ctgggcgatg aaggctgggc
541 tacgaactcg tagcttgcga ccgcaacgcg atccctgtcc agcgtgacga tcggaccttg
601 cgaactcaaa cgtggttgag cgaataacca tttgcctcag tccttcgcgg atcgccgcac
661 gtgttccacg aaccttgcca ggatgggcgg ctgtttctcc ttgcggaagg tgcagctgat
721 gggcaccttg acccgtgtcc cgacgatccg cgcgaacgcg atatccggcc aacggatcgc
781 tgcgacagac gcagggacga tgctcgacgc cgcaccggcc atggtcaggg cgagcgcggc
841 cgtggcgtcc tccacgactc gggcgatgcg gggctcgata cccgcgtgct tgaacaggcc
901 gatcacctca tcggcgaagc tcggccggcc gccgcgcgga aacagtgtga gttccaccgc
961 gcgcaggtcc gcgagcttgc aggtcttgcc gaacttcccc gactgggagc ggtgcaccgc
1021 gaggtagaga tcctcttgcg cgatgttgac gatctcgatc ccgggatgcc ggggaaagaa
1081 gcggctgaag cccacgtgga tggtccccgc gagcaggccc tccacctgct cgtccttggt
1141 catgtgcgtg agggataccg tcgccgtagg cgtggacgta aggaaagcgc gcagcaaaag
1201 gggcaggctg cggtagatgg gcgttccgaa atacgccacg ctcaactcgc ccacgtcgcc
1261 ccgcgcggcg gcgcgggagc gatcacccga acggcctgcc aactccagga tgcggcgcgc
1321 atcctccagg aaagcgtgac ctgcggcggt gagttcgatc ccgcggtggc ttcgctccag
1381 aagcacgacg cccaagtccg cttccagggc ttgcatctgc cgcgtgatgg ggggctgtga
1441 gacgtgcagg cgcttggctg ctgcagccat gttccctgct tccgcgacgg cgatgaaata
1501 cttgagctgc cggaattcca tggcgcgctt tccgagttgg tgatgtgcct atattacgca
1561 aaccgtaacg atggctgact aatttggtat tggacggcat aggccggcgc tgcctagcat
1621 tcactcgtcc cgcgtcaatc cggaccgggc aaaccgacaa aggagaccgg gatgaacgaa
1681 cgagtgaagc aggttgcgtc cgcattggtg gatgcgatcc agaagaccct cacggagcaa
1741 cgcgtgacgg aagaagagtg gcgcgcgggc gtcggctaca tgatgaaact ggccgaggcc
1801 aaggaagtgg ctgtcctgct ggacgcgttc ttcaaccaca cgatcgtcga cctcaaagcc
1861 caggccacgc gcggatccag gcccgcgatg cagggcccgt atttcctgga aggcgccccg
1921 gtggtcgccg gcgccctgaa gacctacgag gatgactcgc atcacccgct cgtgatccgc
1981 ggcgccgtgc gcacggacga tggcgcgcca gccgcgggag ccgtgatcga cgtgtggcac
2041 tccacgcccg acggcaagta cagcggcatt catgaccaga tcccgaccga catgtaccgc
2101 ggcaaggtcg tggccgatgc gcagggcaag tacgccgtgc gcaccaccat gcccgccccg
2161 taccagatcc cgaacaaggg accgaccggc gtgctgcttg aaatgatggg aagccatacg
2221 tggcgtcccg cgcacgtgca cttcaaggtg cgcaaggacg ggttcgcgcc gctgaccacg
2281 cagtactact tcgaaggcgg cgactgggtc gacagcgact gctgcaaggg tgtcgcaccg
2341 gacctggtca tgccgacaaa gaccgagggc ggcgcgcagg tgatggacat cgacttcgtc
2401 atcgagcgcg cgcgggagca cgtatgaaaa tcgaggcgat tagcacgacg atcgtcgatg
2461 tcccgacacg tcgcccgttg cagatgtcgt tcaccacggt gcacaagcag agctatgtga
2521 tcgtccaggt gaaggccggg ggcctcgtgg ggatcggtga aggcggtagt gtcggcgggc
2581 cgacctgggg ctccgaatcg gccgagacca tcaaggtcat catcgacaac taccttgcgc
2641 cactcctggt cggcaaggac gcgtccaacc tgagccaggc aagggttctc atggatcgcg
2701 cggtcacggg caacctgtcg gcgaaggccg ccatcgacat cgcgctgcac gacctgaaag
2761 cgcgcgcgct gaacctatcg atcgccgact tgatcggcgg gacgatgcgg accagcatcc
2821 cgatcgcatg gacacttgcg agcggcgaca ccgcccggga catcgactcg gcgctcgaga
2881 tgatcgagac gcgacggcac aatcgcttca aggtgaagct cggcgcgcgc acgccggctc
2941 aggatctgga gcacatccgc agcatcgtga aggccgtggg cgacagggct tccgtgcgtg
3001 tcgacgtaaa ccagggatgg gacgagcaga ccgcttcgat ctggattccg cggcttgaag
3061 aagcgggcgt ggaactggtc gagcagcccg tgcctcgcgc gaatttcggg gcccttcggc
3121 gtttgaccga acaaaacggg gtggcgatcc ttgccgatga gagcctcagc tcgctgtcgt
3181 cggcattcga gctggcccgc gaccacgcgg tggacgcgtt ctcgctgaag ctttgcaaca
3241 tgggcggcat cgcgaacacg ctaaaggtcg cggcggtcgc ggaagcagcg ggcatttcct
3301 cctatggcgg aacgatgctg gactccacgg tcggcacagc agctgcactg cacgtgtacg
3361 cgacattgcc ttccctgccc tacggatgcg agttgatcgg cccttgggtt cttggtgacc
3421 gcctgaccca gcaggacctc gagatcaagg acttcgaagt gcacctgccg ctgggaagcg
3481 ggcttggcgt ggacctagac cacgacaagg tccgtcacta cacacgggcg gcctgacatg
3541 cactcctcga aatgccctga cctcgcgaac atcggccgcc gtcgcgtcct tgcgggcatc
3601 gcgctcgcaa tgacgacaag ttcaaccagg gcacaacggc ttgacgcctt tccttcgcgt
3661 cccatcacat gggtagttcc cttcccgccg ggcggagccg tcgatgcgat cgcgcgcatc
3721 atttcgcgaa agatgtcaga gagcatcggg cagcaggttg tggtggataa ccgcgcgggt
3781 tcgggcggca tcatcggcag cgacctcgtc gcgaaggccc cgtctgacgg acacaccatt
3841 ctgatcaact ccacgggact ggtggtggac cggttcttct atcctcgcgt accgtaccag
3901 tccgatcgcg acttcgttcc ggtggttctc gcggcgacgc tccccagcgt tctggtcgtg
3961 ccggcggatt cgcggtttcg tgacatcacg cagctcctga aagcggcgcg cgaaaacccc
4021 ggcaagttgt cctttgcttc cgcgggattg ggcacgtcta tccaccttgc ctccgcgctc
4081 ctcgcggcca gggcgaacgt ggagctgctc catgtcccct accggggcag cagtccagcc
4141 gtttcggacc tggtcgcggg ccgcgtcgac atgatgatcg actcagtgac atcgcagcgc
4201 cagaacatcc tcgccaagcg cgttcgcgca ctcggggtga cgagcctgga tcgccatccg
4261 caactgcccg aagtgccgac catcgccgag gcggcgggcc ttcccgggtt tgaagtgctc
4321 acatggtgcg gggtgttcgc acccaaaggc acgcctcgct ccgtcgtgga gcggcttaac
4381 gccgaaatca acaaggcgat tgctgcgccg gacgtcgtcg aagcacttaa gcagcttggg
4441 atcaagactg ccggcggcgc accggaagtc cttgcggacc tattcaagtc cgagacggag
4501 cggtggcaaa agctcatcgt cgaataccgc ctcaatgcaa accagtgaag ttgaaagaga
4561 gacaagcaca tgctgactga aggcttatct atcgatgcga aaggaggcgg ccgcttcggc
4621 gcgcacctgc aattgccggc acgcgggcgc ggcccggtgg tgatggttgc gcaggagatt
4681 tttggggtca acccattcat gaccgaagtc ttggcgtggt tggcatcaga aggttttgtg
4741 ggcctctgtc ctgatctgta ttggcgccac ggaccaggca tcgagttcga tccgaacgac
4801 gaagtacaac gtgcgcgcgc gctcgggatg ttccgcgact acaagctgga agatggggtg
4861 gcggatttgc gggccacagt cgcctacgcg gcttctcagc cgttttgcga cggcggggtc
4921 gcggtgatcg gctattgcct gggcggagcg ctggcctacg aagttgccgc agagggattc
4981 gcgcaatgct gtgtcggcta ctacggcgtc ggcttcgaga agcgtctcga acgcgcccga
5041 cttgtgaaga caccatcaat gttccacatg ggcacgaacg accatttcgt gaccgcggag
5101 gccagacaac tcatcacgaa tgcctttgaa gcgaatcccg cgattgcgct gcattggtac
5161 gacgccgggc actcgttcgc gcgggcttcc agcccgaact tctcaccgga ggccacgagg
5221 acggcgaatg cacgcacgct ggaaatgctg aagcgcatga agcccatcgg gacaattggc
5281 caatgaactt catccacgac ccactcacgc ctcgcgtctt gttcggcgcg gggcgcctgc
5341 agtctctggg cgaagaactg aagctgctcg ggatacggcg cgtgctggtt atctccaccc
5401 cggagcagcg tgaactcgcc aaccaggtcg cggccctcat tcccggcagc gtcgccggat
5461 tcttcgaccg cgcgacgatg cacgtgccgt cgcaaatcgt cgatcaggcg gcttctgtcg
5521 cgcgagaact cggcgttgac agctatgtgg cccctggcgg aggttcaacc atcggcctcg
5581 cgaagatgct cgcgctccac tcgtcattgc caatcgtggc catccccacg acgtatgccg
5641 gaagcgagat gacatccatc tatggcgtca ccgaaaacga attgaagaag accggtcggg
5701 ataggcgcgt cctcgcgcgc acggtcatct acgacccgga gctgacattc ggcctgccca
5761 cgggcatcag cgtgacaagc ggtttgaacg cgatcgcgca cgcggttgag ggcctttacg
5821 cgccggaggt gaacccgatc ctcgcgatca tggcccagca aggaatcgcg gcgttggcca
5881 aatcaattcc cacgatccgt tcggctccga ccgaccttga ggcccgttcg caagcgcaat
5941 acggtgcatg gctgtgcgga agtgtgctcg gcaatgtcag catggccctg caccacaagc
6001 tgtgccatac gttgggggga acattcaatc tgccgcacgc tgaaacgcac actgtcgtgc
6061 tgccgcacgc gctcgcctac aacacgccgg ccatcccgcg tgcgaatgca tggctgcagg
6121 aggcactcgc cactcgcgaa ccggcgcagg cactgtttga tctcgcgaag tcgaacggcg
6181 cgccggtttc actgcaatcg atcgggatga aggaagcgga cttggaccgc gcctgcgagc
6241 tggtgatgtc cgcgcaatac cccaatccgc gaccgctcga aaagcatgcg atcgcgaatc
6301 tgctgcgcag ggcctatcta ggggagccac cgcaaccctg accttgccgc aggccaccgt
6361 tcggtggcat attgattgct tgggaaaaca atgcgccccc gcctatgtag ctcgcggcgc
6421 aaatggagac tgccatgcac caacaaggtt catctttcga cctgtcgaat ctgcagcgca
6481 actgcatttt ccgatgcgaa tcgccgatgg agtcgcacga gaagatcgcg cacgaattgg
6541 ccgaccacga tttgcgctgg cggcggggcg ccgttgacac cggcatgtac aaggtcgggg
6601 ttcacgcctt ggaaatgttc atgctgcact atggcgccga agtggaggtg cggccccgcg
6661 ttcccgaagg gttcgcgctg gtccatctct cgttgaaagg cgcgatcgac atcatctccg
6721 acgagcagcg catcacggtt tcgcccggcg aagtcgcgta tctcgcgccg cgcaagaatt
6781 tttcggatgt tgtggcagcg cggtgcacag cagctgattc tgaaggtgcc cgacaccttg
6841 ctgcgttcca ttgtggccga ggcgggcgcg tgcctggact tgccgtcaac catgagattg
6901 acgccggcag ccgccaggca atggcacttg ttcatgcaat cgctgctcaa catcggtacc
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