[bionet.molbio.genbank.updates] Plasmid pFC10

GenBank-Updates@genbank.bio.net (05/15/91)

LOCUS       PCFPRGAB    11958 bp ds-DNA             BCT       15-MAY-1991
DEFINITION  Plasmid pFC10 (from Enterococcus faecalis) regulatory protein
            (prgX), surface exclusion protein (prgA), aggregation substance
            (prgB) and surface protein (prgC) genes, complete cds.
ACCESSION   M64978
KEYWORDS    aggregation substance; prgA gene; prgB gene; prgC gene; prgX gene;
            regulatory protein; surface exclusion protein; surface protein.
SOURCE      Plasmid pCF10 (from Enterococcus faecalis) DNA.
  ORGANISM  Plasmid pCF10
            Prokaryota; Bacteria.
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Dunny,G.M., Olmsted,S.B., Kao,S.-M., Viksnins,A.S., Gallo,J.C. and
            van Putte,L.J.
  TITLE     Molecular and genetic analysis of the EcoRI c fragment of the
            plasmid pCF10 containing positive control genes and structural
            genes encoding surface proteins involved in pheromone-inducible
            conjugation in Enterococcus faecalis
  JOURNAL   Unpublished (1991)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             complement(697..1650)
                     /product="regulatory protein"
                     /gene="prgX"
                     /codon_start=1650
     misc_feature    699..5330
                     /note="positive regulatory region"
     CDS             3681..6356
                     /product="surface exclusion protein"
                     /gene="prgA"
                     /codon_start=3681
     CDS             6549..10466
                     /product="aggregation substance"
                     /gene="prgB"
                     /codon_start=6549
     CDS             10912..11769
                     /product="surface protein"
                     /gene="prgC"
                     /codon_start=10912
BASE COUNT     4544 a   1993 c   2367 g   3054 t
ORIGIN
        1 gaattctgag aattttgagc ctatttttga acagctacaa gaagcatatc cagaactgtg
       61 gaaagttctg ttagtagaac gagatcaata tttagctcag aaaatttcag atcaggaagc
      121 tggtaaaatc gtagttgtta ttgggcaggc gcatttacgt ggtgtggaga aaaacttaaa
      181 aagtaatcaa caaatcaata taaaagaatt agaaacttta ccgccaaagc tttggtcaac
      241 taaagtttta gagagtatca tacctttgat tatcataggt ttgttaattt attccttttt
      301 cttaggtgta gatgtaggta aagaccaaat aatgagatgg ggtatttgga acggtggttt
      361 atcagcacta tttactttat tagcgttggc tcacccctta acaattgtca cttccctagt
      421 gtttgccccc cttgctacct tgctaccaat ggtgtctgta ggagtgtttt ctgctattgt
      481 agaggctact ttaaggaaac caaaagtacg tgattttcaa acgatggatg aagatataaa
      541 atctttgaaa aagatatata aaaaccgtgt tttgagagta tttttagtgt tcgtgttagc
      601 tagtttggga ggaatgattg gtaattttgt tggtggcttg gaaatgttta ggcaccttat
      661 ataataaaaa ataaaagagc agtcatgaaa atattttcat gactgctctt ttatttcagg
      721 aataggatta ttttctattg caacgtaatt ttcataatac atggttactt ctttaggggg
      781 cgtaaatttt tcttttgcag atatttttgt aagttcctca actaaagatc tgtgaatatc
      841 ttcttttcca atgattttaa agatgttgat aatatttacc gcatttaaat aagaatcaat
      901 atttttgtca gttaaaaatt gatatatttg ttttaaatag tttatttcta agtcgtaata
      961 tccatttatg ctaatatttt taattgtttt taagtgttca aattgattaa tataatactg
     1021 cgcttctttt agatttctat tcataattga tattgttaaa gcatttttta gaacagtctg
     1081 aatagttaaa tcgtaatctt taccaaagga atccacgatt ggatacatag gcttaatgat
     1141 acttgaaact tcttcatacg gtaagacatt taaaagattt gaaacaattt cataatcaat
     1201 tccaaaaaat gttgttcttt tatcatataa atgttttaaa tctgaagtaa ttgttttatt
     1261 aaaagtagga acttcaatgt tataatgatg tgcaatagaa atatatccaa gataaatact
     1321 aaagtactga agactagtta agcgctttgg ctcaatcctt tgaaaatttt tgtcaaataa
     1381 gtcagggttt gtaaatattt ttgaaataag aagtttttct tttcctgttt catttacgct
     1441 ttttgtattc atgccagctc tatttaaaat ttcaaaaaaa tttacaccta aacgttctga
     1501 gaacttgctt aactcttcta cagaaatcgg tcgagagtca gcttcaactt ttatgtaaac
     1561 agatttggac attattccag aatataaatc aatctgatgg taatttaact cttgccttat
     1621 ttgtttcagg acagaaccta tcttaaacat tttcttcctc ctaatatgta aagtatcaaa
     1681 aagtagacct aaaattcgat aaactacaaa aatttgttaa tattttaatt ttaggtattg
     1741 aatacgacac tcgaagatgt gtttattaag ctatatccct tttttttaaa aaaaaataca
     1801 tattttagtt gaaaatataa tacttagatg ttaagatgtt tttataggag gggtgtaaat
     1861 gaaaaccact ctaaaaaaac tatcaagata tatagctgtt gtaattgcaa taaccttaat
     1921 atttatctga tagaaaaaat catagtaaca attaaacaaa ttaagaaccg actgccatag
     1981 gacggcaatc ctagggggca gttaaacaat tcatggtatg acatgaactc tactcggttc
     2041 tcgtttgttg caacattagt tacaacgtat agtataacaa ttttttatgt aaaattctag
     2101 acttttttaa actcctttat ttgtctagga aaagttttta cagtgaattg tttttattag
     2161 ttgtataaat gttggagcag cggggaatgt atacagttca tgtatatgtt ccccgctttt
     2221 tttgttgtct gttaacttgt tgaaaaaata aaaaaaccaa gtaacaagca cgctcatttt
     2281 ctaaaaatga cccgcttgtg gaataattca atgctttcac gcattgtttg tactggaact
     2341 actcgtaaga gccgtgcaaa caacgtgttg catggctctt tttctacttg gttcagaaag
     2401 taggtgaatc aatgattgaa ctgaaagcaa ctgatttgca acgtattacg tgtcaattga
     2461 ccttgtttaa tcgacaaaga tttaaattat acaacggcac aacagaacgc attgtatatg
     2521 atttttcagg aagaaatctg ttgatcaatc ctgtgagttt tgaaacggaa caagacatgg
     2581 aacgtttatt cagacaagta aaactgattt attttgatgg aaaagtggtc ggttatcgtg
     2641 gctgctcgga acttccgtta aaacttgaat tagaacgttc aaaaaaatgg ttaaacgaca
     2701 aaagatgtta cttaatgcac cattcaacta taaggttttt gaagaaaatg aatttaggga
     2761 atggtgtgaa aaaatgagaa gctataagta gtgaatttgt ttttttatac ttggtctttt
     2821 tgtttgttag ttttaactaa catgcgcaat ttaccaatag ctaggttagc tatcgaaaaa
     2881 ggatgcagaa aagcacacgg acacgaaggt gcaaactggc tacacgtcaa cttttttgca
     2941 aaatttggga gatccttaaa aaatgaatac tacaggactt atctagtcaa tcagcgtgtc
     3001 tttaaagggc taggtaagga taaactacct aaagagtacg gtcagaacaa aggcggtacg
     3061 tgattatggg tgtgacacag taactaaaaa gatttcgtgc gatgatcttt tgttttaaag
     3121 ggagccagat acttttttag agtttggggt tgctaccgct acaaaccctg cgatcagtaa
     3181 aatctgtaga aaaattgcta aaaagctagc aaacaaaagg atcaagtaaa acgaggatat
     3241 ttaccataaa accaatagga ggaaagtgaa catgaagaca taggagtgtt tttctaaggg
     3301 ttccaaggat agtatttttt taataatgaa ggctttgtta ctattaattt gaggtgagag
     3361 gggtatgaca aaaaaagagc aaagtatttg gcgtaaagag atgttggcat tgatgaatga
     3421 agacgcagac tggtatagga atgaagatac cgagcgtttt aaacgaatac aagaacttgc
     3481 aaaaaagata gaaacagcaa gcaccagaca attttcttct catattagca aggaaagatt
     3541 tgaagcctat taaagaatgg gcttacagtt taaagagata gcggaagagt tccatataac
     3601 aactacagcg ttacaacaat ggcataaaga caatgggtat cccatttaca acaaaaataa
     3661 tcggaagtga ggaaaataaa atgaaaaaga ttgcaagtac agggttaagt attttagtcg
     3721 caacaggggt agcaggtatt gggggaaatg aagtacaggc agcagaacaa gcgcaaccaa
     3781 aaacacctga aaacagttct acagaacaac caacagtgaa agctacacaa acaacggagc
     3841 aagctattac tgaaaaacag caacaagtaa cagagaaaca agcaattgtc gatcaaaaac
     3901 aacaagtcgc tgacactgcg aaaaaagaaa aagacgccat tgatcaatct gttaaagacc
     3961 aacaagcagt ggtcgatcaa aacaaagacg cattggatca aagtcaacaa gcagtgactg
     4021 accaacaagc agttgtagac gaagctaaaa aagtcgtgga tgaagcaaca ccttcagcca
     4081 ttgaaaaagc taaagagcaa gtggctacgg atacacaggc tgttgatgaa caacaaaaag
     4141 tagtggatca agctcaaaca gacgttaacc aacaacaagc tgttgtcgat gaaaaagcaa
     4201 aagaaacgaa tgctgctaaa gtgcaaaatg aaaaagatca acaagcagta acagctgcaa
     4261 aacaagaaca agccaagctt gaagaattag cgaaaaatgc ggaagcggaa aaagtaaagg
     4321 cagaaaaaga acaagcagca aaagaagctg aactggctaa caaacagaaa gaagaagcaa
     4381 aagctaaaga tcaaaagacg aaggacgatc aagcagttgc agaccaacaa accgttgtga
     4441 caactagtca agagaaagta acagacgcaa aagcagatac agctgcgaaa caggcagatt
     4501 taactgcgaa agaaaatgct ttgaaagaca agcaagcagc aacaaagcaa gcgcaaaata
     4561 ctttagataa ctcaaaagaa gagttgaaag ggcataaagg gattaacttg ccacctaagt
     4621 ttagtgcaga ttacgataca aaattaagtg ctgaagaaat agctactctt gaaaaaacgg
     4681 cactagaaat gaacaagaat tttccaacga gcaaagaaga tgaaaaaaat aaagacgtga
     4741 tgtgggatat tcaacattta agtgcagatc agaaaaaaga attaagtgta tacacaacag
     4801 aattattaaa tgatgtgcga aaaaaattgg gtctttctca attaagtgta tcggaccaat
     4861 caatcaaatt tgcttgggat attgcgaaat attctgatac cggagaatac atgcatgatg
     4921 tgatagccat taataaagca gctaaggaaa atggctttaa agaatatcct ggaatgaatt
     4981 actatgaaaa cctaggtggg gggtattacg aaactgaaaa tggtaaagtt tctaaatata
     5041 ctctccaaga aagtatccgc aaaatgcttg tcaatatgtt gtttgatgat ggccgcttag
     5101 gttatagcca tttacattct ttattacaag acggaaaaac agcattaggc gtttcactat
     5161 ctggtgaaaa aaattctatt tctcctaaga ttcatattat ttcttatggt aaagaaaaac
     5221 tggaggatag tagtcaatac caaaacggcg aagtggcaag catgaaatct aaagaagaat
     5281 tacaacaaga gatagctagc aaccaagaaa aattagctac cgcacaacaa gcagaatcag
     5341 acgcacaaca agcaagaagt gcaagtcagc aagccttaaa tacagccaaa acaacacaag
     5401 caacagcaga aaaagaacta tctgttcata aagcgacatt ggctaatctt caagcagttg
     5461 cgactaaaag tacgacaaac tatgaagaaa aagtacgaca aactgcaacg gcagaaaaaa
     5521 gccttcaaca aacaaaagat caattggcaa caatcaatga gttaattcag aatcgagctg
     5581 ctgttttaga aaaagcgaaa acaaacgttg cggaagcaca agcgattgaa caaacgtctg
     5641 ctaaagtgct gaaagaaaaa caagaagcac aaaaagcaga agaaaacaca ttgaatagct
     5701 tgaaggaagt attggattta gcaaaagaaa acttaaatca aaaacaagta gcgcttaaaa
     5761 caagtacacg ttcattgtct cgtttagaaa atgctcaacc aacatacgaa aaagcattaa
     5821 acgagttaaa caaagcagaa gcggcagtgg ttcaagcaca agaagcctat gaaaattcta
     5881 tgaaatcatt ggaggaactt aaagaacaac aagccgttgc tacacttgct tatgcacaag
     5941 cacaagaaga cctttctaat gcgaagttag agctacagca gtaccaaggt gtattaagag
     6001 acttagaggc acaacaagcc gaacagcgcc gacaagaagc gttgcaagag caagtagcaa
     6061 aagagcaaca acgccttgaa cgagaagcaa aacaaaacca aacgttagta gcaagtgcta
     6121 cttcagcaga caaaacacct ggtctccaac agttatcttt ttctaaacaa aaagaacaac
     6181 caaaagcaca agcattaaca cattcagaac ctcgtaagac gaaacaagta gcaaaagcac
     6241 cagaatcctt accacaaacg ggagaacaac aaagtatttg gttgactatt attggattat
     6301 tgatggcagc aggtacgatc aatttcaaga acaaaaaacg taaaaaaaat agttaataat
     6361 tgtttgatat agcaaaacaa agatatatat cgtgttttat aagaatataa aataactaag
     6421 agaatcgaag atactttcgg ttctcttttt ttagtaagga gggaaatgac aatgaaaaag
     6481 caacgagcgc cttgtgttgc gtgagttatt ttacaaacaa aaaaataaaa aatcgaggag
     6541 aatgatacat gaatcaacag actgaagtaa aaaaacgttt taaaatgtat aaggcaaaga
     6601 agcattggat cgtagctcct attctttttc taggcgtgtt aggagttgta ggattagcta
     6661 ctgataatgt acaagcagcg gaattagata cgcaaccagg atcaacgaca gtgcaccccg
     6721 ataaccccga tccgcagtta ggtagtgcaa cgcctaatac ggcagtatct gaagaatctg
     6781 cagtacaaaa agacactact tctcaaccga ccaaagcaga agaagtagcg tctgaaaaaa
     6841 atggagctga acagagttca gctaccccaa atgataccac aaacgtccaa caaccaacag
     6901 taggagctga aaaatcagca caagaacaac cagtagtaag ccctgaaaca accaatgaac
     6961 ctctagggca gccaacagaa gttgcaccag ctgaaaatga agcgaataaa tcaacgtcca
     7021 ttcttaaaga atttgaaaca ccagacgttg ataaagcagt tgatgaagca aaaaaagatc
     7081 caaacattac cgttgttgaa aaaccagcag aagacttagg caacgtttct tctaaagatt
     7141 tagctgcaaa agaaaaagaa gtagaccaac tacaaaaaga acaagcgaaa aagattgccc
     7201 aacaagcagc tgaattaaaa gccaaaaatg aaaaaattgc caaagaaaat gcagaaattg
     7261 cggcaaaaaa caaagcggaa aaagagccgt acgagaaaga agtcgcggaa tacaacaagc
     7321 ataagaacga aaacagctat gtcaatgaag cgattagtaa aaacctagtg ttcgatcaat
     7381 ctgtcgtgac gaaagacact aaaatttcgt cgattaaagg cggaaaattt atcaaagcaa
     7441 ctgattttaa taaagtaaat gcaggggatt caaaagatat ctttacaaaa ttacggaaag
     7501 atatgggtgg gaaagctact ggcaacttcc agaattcctt tgtaaaagag gcaaatcttg
     7561 ggtctaatgg tgggtatgcg gttcttttag aaaaaaataa accagtgaca gtgacctata
     7621 caggactaaa cgctagttat ttaggacgta aaattacaaa agcagaattt gtttatgaac
     7681 tacaatcctc accaagccaa agtggaacgt taaatgcagt attttcaaac gatccgatta
     7741 tcacagcttt tattggtaca aacagagtca atggtaagga tgttaaaaca cgcttaacga
     7801 ttaagttctt tgatgcgtca ggtaaagaag tactaccaga taaagatagt ccatttgcgt
     7861 atgcgctgtc ttctttaaat tcaagtttaa cgaataaagg tggccatgcg gaatttgttt
     7921 ctgattttgg ggccaacaat gcgttcaaat acattaatgg ctcatatgtg aaaaaacaag
     7981 cggatggaaa attttactca ccggaagata ttgactatgg cacaggacct tctggattga
     8041 aaaatagtga ttgggacgct gtagggcaca agaatgcgta ctttggttca ggtgtaggtc
     8101 tagctaatgg acgtatttcc ttttcttttg gtatgacaac aaaaggaaaa agtaatgtgc
     8161 ctgtatctag tgcgcaatgg tttgccttta gcactaactt aaatgcgcaa tcagtgaagc
     8221 ctattttcaa ttatgggaat ccaaaagaac cagaaaaagc aacgattgaa tttaatggat
     8281 acaaagccaa tgtcgttcct gttcttgttc cgaacaaaga agtcactgat ggtcagaaaa
     8341 atatcaatga tttaaatgtg aaacgtggcg actctttaca atacattgtg acaggggata
     8401 caacagaact tgctaaagta gatccaaaaa cagtaacaaa acaagggatt cgagatacat
     8461 ttgatgcaga aaaagtgacg attgatttat ccaaagtgaa agtttatcaa gcagacgcaa
     8521 gtctgaacga aaaagactta aaagcagttg ctgcagcgat taattcagga aaagcgaaag
     8581 acgtgactgc ttcttatgac ctcaatttag atcaaaacac cgtcacagca atgatgaaaa
     8641 ccaacgcgga cggctctgtt gttttagcaa tggggtataa atatttactt gtcttgccgt
     8701 ttgtagtgaa aaatgtagaa ggcgattttg aaaatacagc tgttcagctg acaaatgatg
     8761 gggaaacggt aacaaacaca gttatcaatc atgtgccaag tagcaaccct tccaaagacg
     8821 taaaagcaga taaaaacggt acagttggca gtgtctctct acatgataaa gatattccgt
     8881 tacaaacaaa aatttattat gaagtgaaat cttccgaacg tccagccaac tatggcggaa
     8941 tcacagaaga atggggcatg aatgatgtgt tggacacgac ccatgatcgt ttcacaggga
     9001 aatggcacgc tattacgaat tatgacctta aagtagggga caaaacgcta aaagcaggaa
     9061 cagatatttc tgcctacatt cttttagaaa ataaagacaa taaagacttg acgtttacga
     9121 tgaatcaagc attattggcc gccttaaatg aaggaagcaa taaagtaggc aaacaagctt
     9181 ggtctgtgta tctggaagtc gaacggatta aaacaggtga cgtagaaaac acgcaaacag
     9241 aaaactacaa caaagagctt gttcgttcta atacggtggt gacgcataca cctgatgatc
     9301 caaaaccaac caaagccgtt cataacaaga aaggggaaga cattaatcat ggaaaagtgg
     9361 ctcgtggtga tgttctttct tatgaaatga cttgggactt aaaagggtac gataaagact
     9421 ttgcctttga tacagtcgat cttgcgacag gcgtttcttt cttcgatgat tacgatgaaa
     9481 cgaaagtgac accaattaaa gacttacttc gtgtcaaaga ttctaaaggg gtagacatta
     9541 cgaaccagtt cacgatctca tgggacgatg ccaaaggcac ggtgacgatt tctgccaaag
     9601 acccacaagc ctttattcta gcgtatggtg ggcaagaatt gcgtgtaaca ctccctacaa
     9661 aagtcaaagc tgatgtttct ggggatgttt ataattcagc ggaacaaaat acatttggcc
     9721 aacgaattaa aaccaatact gttgtcaacc atattccaaa agtgaatcct aaaaaggatg
     9781 tggtcattaa agttggtgac aaacaaagtc aaaatggtgc cacaatcaaa ttaggggaga
     9841 agttcttcta tgaatttaca agtagtgaca ttcctgcaga atacgctgga gttgtggaag
     9901 aatggtcgat tagcgataaa ctagacgtca aacatgacaa atttagtgga caatggtctg
     9961 tgtttgccaa ttctaatttt gttttagcag atggaaccaa agtgaataaa ggggacgaca
    10021 tttcgaaact attcacgatg acctttgaca aaggggtagt gaaaatcacg gccagtcaag
    10081 cctttttgga tgcgatgaat ctaaaagaaa acaaaaacgt tgcacactca tggaaagcgt
    10141 tcattggtgt agaacgaatt gcggcaggag acgtttacaa cacaatcgaa gaatctttca
    10201 acaatgagaa gattaaaacg aatacggtag tgacacatac gccagaaaaa ccacaaacac
    10261 caccagaaaa aacagtgatt gtaccaccaa caccaaaaac accgcaagca ccagtagagc
    10321 cattagtggt agaaaaggca agtgtagtgc cagaattgcc gaaaacaggc gaaaaacaaa
    10381 atgtcttatt aacggtagtt ggtagtttag ccgcaatgct tggcttagca ggcttaggct
    10441 ttaaacgtag aaaagaaaca aaataattga agacgaaaag aaggacgaat tgcttgtcct
    10501 tcttttttta gttatggagg ggaaaaattg gaagcagtag tagcagaaag agaggcaaag
    10561 gggatgaaag aaattgccat ccaagaaaaa gatttgacct tacagtggag aggaaacaca
    10621 ggtaagttag ttaaagttcg attaaagaat acacgtgcaa tggaaatgtg gtacaacaaa
    10681 caaattaccg aagaaaacat tcaagagatc accacgttga atatcattaa aaacggaaaa
    10741 tctctggcat tagaagtata tccagaaaaa agtatctatg tgaaaccaaa tttaggcaga
    10801 atcaatgtgc ctgtcttttt tatcaaaaca cctattaata gagggatttt tgaagagatc
    10861 ttcggcgaaa ctttaaaatc ataaataaaa aattatttgg aggaaattac aatgaaaaaa
    10921 attattttat caagcttgtt tagtgcagta ctagtattcg gtggcggaag tataacagca
    10981 ttcgctgacg atttaggacc aacagatcca gcaactccac caattaccga accaactgat
    11041 tctagtgaac ctacgaatcc tactgagccg gtggatcctg cagaaccgcc agtaatacca
    11101 actgatccaa cagaaccaag caagccaacc gagcctacaa caccgagtga gccagaaaag
    11161 ccaacagaac caacaacgcc aattgatcct ggaacgccgg ttgaaccgac tgaaccaagc
    11221 gagccaacag aacctagtca accaaccgag cctacaacac caagcgaacc agaaaaacct
    11281 gttactccag aaccaccgaa agaaccaact caaccagtga ttccagaaaa accagcagaa
    11341 ccagaaacac caaaaactcc tgaacagccc actaaaccaa tagacgtagt cgttacacct
    11401 agtggagaaa ttgataaaac gaatcaatcg gcaggaacac aaccaagtat tcctattgaa
    11461 acaagcaact tagcggaggt aacacatgta ccaagtgaaa ctactccaat tacaacagaa
    11521 gctggggaag aaattgtagc agtagataaa ggtgttccgt taaccaaaac accagaagga
    11581 ttaaaaccaa ttagcagctc gtataaggtt ttacctagcg gaaacgttga ggtaaaagca
    11641 agtgatggaa aaatgaaagt attgccacat acaggagaga aattcacact ccttttctct
    11701 gtattgggaa gcttctttgt attaatttca ggattctttt tctttaaaaa gaataagaaa
    11761 aaagcttaat cagaaagagt aatttcgata aatttctgtg aggaaaggag gtgctttgct
    11821 tgtttatttt atttactaca aagcaaagtc atgtattact aaatgaatta gggtttaata
    11881 gaaaaattga cctggtacaa ggtattgaac agttaacaaa aatcaatgcg gatttaaaaa
    11941 aatcgacgga tcgaattc
//

GenBank-Updates@genbank.bio.net (05/17/91)

LOCUS       PCFPRGAB    11958 bp ds-DNA             BCT       17-MAY-1991
DEFINITION  Plasmid pFC10 (from Enterococcus faecalis) regulatory protein
            (prgX), surface exclusion protein (prgA), aggregation substance
            (prgB) and surface protein (prgC) genes, complete cds.
ACCESSION   M64978
KEYWORDS    aggregation substance; prgA gene; prgB gene; prgC gene; prgX gene;
            regulatory protein; surface exclusion protein; surface protein.
SOURCE      Plasmid pCF10 (from Enterococcus faecalis) DNA.
  ORGANISM  Plasmid pCF10
            Prokaryota; Bacteria.
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Dunny,G.M., Olmsted,S.B., Kao,S.-M., Viksnins,A.S., Gallo,J.C. and
            van Putte,L.J.
  TITLE     Molecular and genetic analysis of the EcoRI c fragment of the
            plasmid pCF10 containing positive control genes and structural
            genes encoding surface proteins involved in pheromone-inducible
            conjugation in Enterococcus faecalis
  JOURNAL   Unpublished (1991)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     misc_feature    697..3553
                     /note="positive regulatory region"
     CDS             complement(697..1650)
                     /product="regulatory protein"
                     /gene="prgX"
                     /codon_start=1650
     CDS             3681..6356
                     /product="surface exclusion protein"
                     /gene="prgA"
                     /codon_start=3681
     CDS             6549..10466
                     /product="aggregation substance"
                     /gene="prgB"
                     /codon_start=6549
     CDS             10912..11769
                     /product="surface protein"
                     /gene="prgC"
                     /codon_start=10912
BASE COUNT     4544 a   1993 c   2367 g   3054 t
ORIGIN
        1 gaattctgag aattttgagc ctatttttga acagctacaa gaagcatatc cagaactgtg
       61 gaaagttctg ttagtagaac gagatcaata tttagctcag aaaatttcag atcaggaagc
      121 tggtaaaatc gtagttgtta ttgggcaggc gcatttacgt ggtgtggaga aaaacttaaa
      181 aagtaatcaa caaatcaata taaaagaatt agaaacttta ccgccaaagc tttggtcaac
      241 taaagtttta gagagtatca tacctttgat tatcataggt ttgttaattt attccttttt
      301 cttaggtgta gatgtaggta aagaccaaat aatgagatgg ggtatttgga acggtggttt
      361 atcagcacta tttactttat tagcgttggc tcacccctta acaattgtca cttccctagt
      421 gtttgccccc cttgctacct tgctaccaat ggtgtctgta ggagtgtttt ctgctattgt
      481 agaggctact ttaaggaaac caaaagtacg tgattttcaa acgatggatg aagatataaa
      541 atctttgaaa aagatatata aaaaccgtgt tttgagagta tttttagtgt tcgtgttagc
      601 tagtttggga ggaatgattg gtaattttgt tggtggcttg gaaatgttta ggcaccttat
      661 ataataaaaa ataaaagagc agtcatgaaa atattttcat gactgctctt ttatttcagg
      721 aataggatta ttttctattg caacgtaatt ttcataatac atggttactt ctttaggggg
      781 cgtaaatttt tcttttgcag atatttttgt aagttcctca actaaagatc tgtgaatatc
      841 ttcttttcca atgattttaa agatgttgat aatatttacc gcatttaaat aagaatcaat
      901 atttttgtca gttaaaaatt gatatatttg ttttaaatag tttatttcta agtcgtaata
      961 tccatttatg ctaatatttt taattgtttt taagtgttca aattgattaa tataatactg
     1021 cgcttctttt agatttctat tcataattga tattgttaaa gcatttttta gaacagtctg
     1081 aatagttaaa tcgtaatctt taccaaagga atccacgatt ggatacatag gcttaatgat
     1141 acttgaaact tcttcatacg gtaagacatt taaaagattt gaaacaattt cataatcaat
     1201 tccaaaaaat gttgttcttt tatcatataa atgttttaaa tctgaagtaa ttgttttatt
     1261 aaaagtagga acttcaatgt tataatgatg tgcaatagaa atatatccaa gataaatact
     1321 aaagtactga agactagtta agcgctttgg ctcaatcctt tgaaaatttt tgtcaaataa
     1381 gtcagggttt gtaaatattt ttgaaataag aagtttttct tttcctgttt catttacgct
     1441 ttttgtattc atgccagctc tatttaaaat ttcaaaaaaa tttacaccta aacgttctga
     1501 gaacttgctt aactcttcta cagaaatcgg tcgagagtca gcttcaactt ttatgtaaac
     1561 agatttggac attattccag aatataaatc aatctgatgg taatttaact cttgccttat
     1621 ttgtttcagg acagaaccta tcttaaacat tttcttcctc ctaatatgta aagtatcaaa
     1681 aagtagacct aaaattcgat aaactacaaa aatttgttaa tattttaatt ttaggtattg
     1741 aatacgacac tcgaagatgt gtttattaag ctatatccct tttttttaaa aaaaaataca
     1801 tattttagtt gaaaatataa tacttagatg ttaagatgtt tttataggag gggtgtaaat
     1861 gaaaaccact ctaaaaaaac tatcaagata tatagctgtt gtaattgcaa taaccttaat
     1921 atttatctga tagaaaaaat catagtaaca attaaacaaa ttaagaaccg actgccatag
     1981 gacggcaatc ctagggggca gttaaacaat tcatggtatg acatgaactc tactcggttc
     2041 tcgtttgttg caacattagt tacaacgtat agtataacaa ttttttatgt aaaattctag
     2101 acttttttaa actcctttat ttgtctagga aaagttttta cagtgaattg tttttattag
     2161 ttgtataaat gttggagcag cggggaatgt atacagttca tgtatatgtt ccccgctttt
     2221 tttgttgtct gttaacttgt tgaaaaaata aaaaaaccaa gtaacaagca cgctcatttt
     2281 ctaaaaatga cccgcttgtg gaataattca atgctttcac gcattgtttg tactggaact
     2341 actcgtaaga gccgtgcaaa caacgtgttg catggctctt tttctacttg gttcagaaag
     2401 taggtgaatc aatgattgaa ctgaaagcaa ctgatttgca acgtattacg tgtcaattga
     2461 ccttgtttaa tcgacaaaga tttaaattat acaacggcac aacagaacgc attgtatatg
     2521 atttttcagg aagaaatctg ttgatcaatc ctgtgagttt tgaaacggaa caagacatgg
     2581 aacgtttatt cagacaagta aaactgattt attttgatgg aaaagtggtc ggttatcgtg
     2641 gctgctcgga acttccgtta aaacttgaat tagaacgttc aaaaaaatgg ttaaacgaca
     2701 aaagatgtta cttaatgcac cattcaacta taaggttttt gaagaaaatg aatttaggga
     2761 atggtgtgaa aaaatgagaa gctataagta gtgaatttgt ttttttatac ttggtctttt
     2821 tgtttgttag ttttaactaa catgcgcaat ttaccaatag ctaggttagc tatcgaaaaa
     2881 ggatgcagaa aagcacacgg acacgaaggt gcaaactggc tacacgtcaa cttttttgca
     2941 aaatttggga gatccttaaa aaatgaatac tacaggactt atctagtcaa tcagcgtgtc
     3001 tttaaagggc taggtaagga taaactacct aaagagtacg gtcagaacaa aggcggtacg
     3061 tgattatggg tgtgacacag taactaaaaa gatttcgtgc gatgatcttt tgttttaaag
     3121 ggagccagat acttttttag agtttggggt tgctaccgct acaaaccctg cgatcagtaa
     3181 aatctgtaga aaaattgcta aaaagctagc aaacaaaagg atcaagtaaa acgaggatat
     3241 ttaccataaa accaatagga ggaaagtgaa catgaagaca taggagtgtt tttctaaggg
     3301 ttccaaggat agtatttttt taataatgaa ggctttgtta ctattaattt gaggtgagag
     3361 gggtatgaca aaaaaagagc aaagtatttg gcgtaaagag atgttggcat tgatgaatga
     3421 agacgcagac tggtatagga atgaagatac cgagcgtttt aaacgaatac aagaacttgc
     3481 aaaaaagata gaaacagcaa gcaccagaca attttcttct catattagca aggaaagatt
     3541 tgaagcctat taaagaatgg gcttacagtt taaagagata gcggaagagt tccatataac
     3601 aactacagcg ttacaacaat ggcataaaga caatgggtat cccatttaca acaaaaataa
     3661 tcggaagtga ggaaaataaa atgaaaaaga ttgcaagtac agggttaagt attttagtcg
     3721 caacaggggt agcaggtatt gggggaaatg aagtacaggc agcagaacaa gcgcaaccaa
     3781 aaacacctga aaacagttct acagaacaac caacagtgaa agctacacaa acaacggagc
     3841 aagctattac tgaaaaacag caacaagtaa cagagaaaca agcaattgtc gatcaaaaac
     3901 aacaagtcgc tgacactgcg aaaaaagaaa aagacgccat tgatcaatct gttaaagacc
     3961 aacaagcagt ggtcgatcaa aacaaagacg cattggatca aagtcaacaa gcagtgactg
     4021 accaacaagc agttgtagac gaagctaaaa aagtcgtgga tgaagcaaca ccttcagcca
     4081 ttgaaaaagc taaagagcaa gtggctacgg atacacaggc tgttgatgaa caacaaaaag
     4141 tagtggatca agctcaaaca gacgttaacc aacaacaagc tgttgtcgat gaaaaagcaa
     4201 aagaaacgaa tgctgctaaa gtgcaaaatg aaaaagatca acaagcagta acagctgcaa
     4261 aacaagaaca agccaagctt gaagaattag cgaaaaatgc ggaagcggaa aaagtaaagg
     4321 cagaaaaaga acaagcagca aaagaagctg aactggctaa caaacagaaa gaagaagcaa
     4381 aagctaaaga tcaaaagacg aaggacgatc aagcagttgc agaccaacaa accgttgtga
     4441 caactagtca agagaaagta acagacgcaa aagcagatac agctgcgaaa caggcagatt
     4501 taactgcgaa agaaaatgct ttgaaagaca agcaagcagc aacaaagcaa gcgcaaaata
     4561 ctttagataa ctcaaaagaa gagttgaaag ggcataaagg gattaacttg ccacctaagt
     4621 ttagtgcaga ttacgataca aaattaagtg ctgaagaaat agctactctt gaaaaaacgg
     4681 cactagaaat gaacaagaat tttccaacga gcaaagaaga tgaaaaaaat aaagacgtga
     4741 tgtgggatat tcaacattta agtgcagatc agaaaaaaga attaagtgta tacacaacag
     4801 aattattaaa tgatgtgcga aaaaaattgg gtctttctca attaagtgta tcggaccaat
     4861 caatcaaatt tgcttgggat attgcgaaat attctgatac cggagaatac atgcatgatg
     4921 tgatagccat taataaagca gctaaggaaa atggctttaa agaatatcct ggaatgaatt
     4981 actatgaaaa cctaggtggg gggtattacg aaactgaaaa tggtaaagtt tctaaatata
     5041 ctctccaaga aagtatccgc aaaatgcttg tcaatatgtt gtttgatgat ggccgcttag
     5101 gttatagcca tttacattct ttattacaag acggaaaaac agcattaggc gtttcactat
     5161 ctggtgaaaa aaattctatt tctcctaaga ttcatattat ttcttatggt aaagaaaaac
     5221 tggaggatag tagtcaatac caaaacggcg aagtggcaag catgaaatct aaagaagaat
     5281 tacaacaaga gatagctagc aaccaagaaa aattagctac cgcacaacaa gcagaatcag
     5341 acgcacaaca agcaagaagt gcaagtcagc aagccttaaa tacagccaaa acaacacaag
     5401 caacagcaga aaaagaacta tctgttcata aagcgacatt ggctaatctt caagcagttg
     5461 cgactaaaag tacgacaaac tatgaagaaa aagtacgaca aactgcaacg gcagaaaaaa
     5521 gccttcaaca aacaaaagat caattggcaa caatcaatga gttaattcag aatcgagctg
     5581 ctgttttaga aaaagcgaaa acaaacgttg cggaagcaca agcgattgaa caaacgtctg
     5641 ctaaagtgct gaaagaaaaa caagaagcac aaaaagcaga agaaaacaca ttgaatagct
     5701 tgaaggaagt attggattta gcaaaagaaa acttaaatca aaaacaagta gcgcttaaaa
     5761 caagtacacg ttcattgtct cgtttagaaa atgctcaacc aacatacgaa aaagcattaa
     5821 acgagttaaa caaagcagaa gcggcagtgg ttcaagcaca agaagcctat gaaaattcta
     5881 tgaaatcatt ggaggaactt aaagaacaac aagccgttgc tacacttgct tatgcacaag
     5941 cacaagaaga cctttctaat gcgaagttag agctacagca gtaccaaggt gtattaagag
     6001 acttagaggc acaacaagcc gaacagcgcc gacaagaagc gttgcaagag caagtagcaa
     6061 aagagcaaca acgccttgaa cgagaagcaa aacaaaacca aacgttagta gcaagtgcta
     6121 cttcagcaga caaaacacct ggtctccaac agttatcttt ttctaaacaa aaagaacaac
     6181 caaaagcaca agcattaaca cattcagaac ctcgtaagac gaaacaagta gcaaaagcac
     6241 cagaatcctt accacaaacg ggagaacaac aaagtatttg gttgactatt attggattat
     6301 tgatggcagc aggtacgatc aatttcaaga acaaaaaacg taaaaaaaat agttaataat
     6361 tgtttgatat agcaaaacaa agatatatat cgtgttttat aagaatataa aataactaag
     6421 agaatcgaag atactttcgg ttctcttttt ttagtaagga gggaaatgac aatgaaaaag
     6481 caacgagcgc cttgtgttgc gtgagttatt ttacaaacaa aaaaataaaa aatcgaggag
     6541 aatgatacat gaatcaacag actgaagtaa aaaaacgttt taaaatgtat aaggcaaaga
     6601 agcattggat cgtagctcct attctttttc taggcgtgtt aggagttgta ggattagcta
     6661 ctgataatgt acaagcagcg gaattagata cgcaaccagg atcaacgaca gtgcaccccg
     6721 ataaccccga tccgcagtta ggtagtgcaa cgcctaatac ggcagtatct gaagaatctg
     6781 cagtacaaaa agacactact tctcaaccga ccaaagcaga agaagtagcg tctgaaaaaa
     6841 atggagctga acagagttca gctaccccaa atgataccac aaacgtccaa caaccaacag
     6901 taggagctga aaaatcagca caagaacaac cagtagtaag ccctgaaaca accaatgaac
     6961 ctctagggca gccaacagaa gttgcaccag ctgaaaatga agcgaataaa tcaacgtcca
     7021 ttcttaaaga atttgaaaca ccagacgttg ataaagcagt tgatgaagca aaaaaagatc
     7081 caaacattac cgttgttgaa aaaccagcag aagacttagg caacgtttct tctaaagatt
     7141 tagctgcaaa agaaaaagaa gtagaccaac tacaaaaaga acaagcgaaa aagattgccc
     7201 aacaagcagc tgaattaaaa gccaaaaatg aaaaaattgc caaagaaaat gcagaaattg
     7261 cggcaaaaaa caaagcggaa aaagagccgt acgagaaaga agtcgcggaa tacaacaagc
     7321 ataagaacga aaacagctat gtcaatgaag cgattagtaa aaacctagtg ttcgatcaat
     7381 ctgtcgtgac gaaagacact aaaatttcgt cgattaaagg cggaaaattt atcaaagcaa
     7441 ctgattttaa taaagtaaat gcaggggatt caaaagatat ctttacaaaa ttacggaaag
     7501 atatgggtgg gaaagctact ggcaacttcc agaattcctt tgtaaaagag gcaaatcttg
     7561 ggtctaatgg tgggtatgcg gttcttttag aaaaaaataa accagtgaca gtgacctata
     7621 caggactaaa cgctagttat ttaggacgta aaattacaaa agcagaattt gtttatgaac
     7681 tacaatcctc accaagccaa agtggaacgt taaatgcagt attttcaaac gatccgatta
     7741 tcacagcttt tattggtaca aacagagtca atggtaagga tgttaaaaca cgcttaacga
     7801 ttaagttctt tgatgcgtca ggtaaagaag tactaccaga taaagatagt ccatttgcgt
     7861 atgcgctgtc ttctttaaat tcaagtttaa cgaataaagg tggccatgcg gaatttgttt
     7921 ctgattttgg ggccaacaat gcgttcaaat acattaatgg ctcatatgtg aaaaaacaag
     7981 cggatggaaa attttactca ccggaagata ttgactatgg cacaggacct tctggattga
     8041 aaaatagtga ttgggacgct gtagggcaca agaatgcgta ctttggttca ggtgtaggtc
     8101 tagctaatgg acgtatttcc ttttcttttg gtatgacaac aaaaggaaaa agtaatgtgc
     8161 ctgtatctag tgcgcaatgg tttgccttta gcactaactt aaatgcgcaa tcagtgaagc
     8221 ctattttcaa ttatgggaat ccaaaagaac cagaaaaagc aacgattgaa tttaatggat
     8281 acaaagccaa tgtcgttcct gttcttgttc cgaacaaaga agtcactgat ggtcagaaaa
     8341 atatcaatga tttaaatgtg aaacgtggcg actctttaca atacattgtg acaggggata
     8401 caacagaact tgctaaagta gatccaaaaa cagtaacaaa acaagggatt cgagatacat
     8461 ttgatgcaga aaaagtgacg attgatttat ccaaagtgaa agtttatcaa gcagacgcaa
     8521 gtctgaacga aaaagactta aaagcagttg ctgcagcgat taattcagga aaagcgaaag
     8581 acgtgactgc ttcttatgac ctcaatttag atcaaaacac cgtcacagca atgatgaaaa
     8641 ccaacgcgga cggctctgtt gttttagcaa tggggtataa atatttactt gtcttgccgt
     8701 ttgtagtgaa aaatgtagaa ggcgattttg aaaatacagc tgttcagctg acaaatgatg
     8761 gggaaacggt aacaaacaca gttatcaatc atgtgccaag tagcaaccct tccaaagacg
     8821 taaaagcaga taaaaacggt acagttggca gtgtctctct acatgataaa gatattccgt
     8881 tacaaacaaa aatttattat gaagtgaaat cttccgaacg tccagccaac tatggcggaa
     8941 tcacagaaga atggggcatg aatgatgtgt tggacacgac ccatgatcgt ttcacaggga
     9001 aatggcacgc tattacgaat tatgacctta aagtagggga caaaacgcta aaagcaggaa
     9061 cagatatttc tgcctacatt cttttagaaa ataaagacaa taaagacttg acgtttacga
     9121 tgaatcaagc attattggcc gccttaaatg aaggaagcaa taaagtaggc aaacaagctt
     9181 ggtctgtgta tctggaagtc gaacggatta aaacaggtga cgtagaaaac acgcaaacag
     9241 aaaactacaa caaagagctt gttcgttcta atacggtggt gacgcataca cctgatgatc
     9301 caaaaccaac caaagccgtt cataacaaga aaggggaaga cattaatcat ggaaaagtgg
     9361 ctcgtggtga tgttctttct tatgaaatga cttgggactt aaaagggtac gataaagact
     9421 ttgcctttga tacagtcgat cttgcgacag gcgtttcttt cttcgatgat tacgatgaaa
     9481 cgaaagtgac accaattaaa gacttacttc gtgtcaaaga ttctaaaggg gtagacatta
     9541 cgaaccagtt cacgatctca tgggacgatg ccaaaggcac ggtgacgatt tctgccaaag
     9601 acccacaagc ctttattcta gcgtatggtg ggcaagaatt gcgtgtaaca ctccctacaa
     9661 aagtcaaagc tgatgtttct ggggatgttt ataattcagc ggaacaaaat acatttggcc
     9721 aacgaattaa aaccaatact gttgtcaacc atattccaaa agtgaatcct aaaaaggatg
     9781 tggtcattaa agttggtgac aaacaaagtc aaaatggtgc cacaatcaaa ttaggggaga
     9841 agttcttcta tgaatttaca agtagtgaca ttcctgcaga atacgctgga gttgtggaag
     9901 aatggtcgat tagcgataaa ctagacgtca aacatgacaa atttagtgga caatggtctg
     9961 tgtttgccaa ttctaatttt gttttagcag atggaaccaa agtgaataaa ggggacgaca
    10021 tttcgaaact attcacgatg acctttgaca aaggggtagt gaaaatcacg gccagtcaag
    10081 cctttttgga tgcgatgaat ctaaaagaaa acaaaaacgt tgcacactca tggaaagcgt
    10141 tcattggtgt agaacgaatt gcggcaggag acgtttacaa cacaatcgaa gaatctttca
    10201 acaatgagaa gattaaaacg aatacggtag tgacacatac gccagaaaaa ccacaaacac
    10261 caccagaaaa aacagtgatt gtaccaccaa caccaaaaac accgcaagca ccagtagagc
    10321 cattagtggt agaaaaggca agtgtagtgc cagaattgcc gaaaacaggc gaaaaacaaa
    10381 atgtcttatt aacggtagtt ggtagtttag ccgcaatgct tggcttagca ggcttaggct
    10441 ttaaacgtag aaaagaaaca aaataattga agacgaaaag aaggacgaat tgcttgtcct
    10501 tcttttttta gttatggagg ggaaaaattg gaagcagtag tagcagaaag agaggcaaag
    10561 gggatgaaag aaattgccat ccaagaaaaa gatttgacct tacagtggag aggaaacaca
    10621 ggtaagttag ttaaagttcg attaaagaat acacgtgcaa tggaaatgtg gtacaacaaa
    10681 caaattaccg aagaaaacat tcaagagatc accacgttga atatcattaa aaacggaaaa
    10741 tctctggcat tagaagtata tccagaaaaa agtatctatg tgaaaccaaa tttaggcaga
    10801 atcaatgtgc ctgtcttttt tatcaaaaca cctattaata gagggatttt tgaagagatc
    10861 ttcggcgaaa ctttaaaatc ataaataaaa aattatttgg aggaaattac aatgaaaaaa
    10921 attattttat caagcttgtt tagtgcagta ctagtattcg gtggcggaag tataacagca
    10981 ttcgctgacg atttaggacc aacagatcca gcaactccac caattaccga accaactgat
    11041 tctagtgaac ctacgaatcc tactgagccg gtggatcctg cagaaccgcc agtaatacca
    11101 actgatccaa cagaaccaag caagccaacc gagcctacaa caccgagtga gccagaaaag
    11161 ccaacagaac caacaacgcc aattgatcct ggaacgccgg ttgaaccgac tgaaccaagc
    11221 gagccaacag aacctagtca accaaccgag cctacaacac caagcgaacc agaaaaacct
    11281 gttactccag aaccaccgaa agaaccaact caaccagtga ttccagaaaa accagcagaa
    11341 ccagaaacac caaaaactcc tgaacagccc actaaaccaa tagacgtagt cgttacacct
    11401 agtggagaaa ttgataaaac gaatcaatcg gcaggaacac aaccaagtat tcctattgaa
    11461 acaagcaact tagcggaggt aacacatgta ccaagtgaaa ctactccaat tacaacagaa
    11521 gctggggaag aaattgtagc agtagataaa ggtgttccgt taaccaaaac accagaagga
    11581 ttaaaaccaa ttagcagctc gtataaggtt ttacctagcg gaaacgttga ggtaaaagca
    11641 agtgatggaa aaatgaaagt attgccacat acaggagaga aattcacact ccttttctct
    11701 gtattgggaa gcttctttgt attaatttca ggattctttt tctttaaaaa gaataagaaa
    11761 aaagcttaat cagaaagagt aatttcgata aatttctgtg aggaaaggag gtgctttgct
    11821 tgtttatttt atttactaca aagcaaagtc atgtattact aaatgaatta gggtttaata
    11881 gaaaaattga cctggtacaa ggtattgaac agttaacaaa aatcaatgcg gatttaaaaa
    11941 aatcgacgga tcgaattc
//