GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS RTSTSG 2262 bp ds-DNA BCT 17-MAY-1991
DEFINITION Plasmid Rst1 Tgs fragment ORF-I, and kanamycin resistance gene,
complete cds.
ACCESSION M65202
KEYWORDS kanamycin resistance.
SOURCE Plasmid Rst1 Tsg HincII-S fragment DNA.
ORGANISM Plasmid Rts1
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 2262)
AUTHORS Mochida,S., Tsuchiya,H., Mori,K. and Kaji,A.
TITLE Three short fragments of Rts1 DNA are responsible for the
temperature-sensitive growth phenyotype (Tsg) of host bacteria
JOURNAL J. Bacteriol. 173, 2600-2607 (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
-35_signal 75..80
-10_signal 104..109
CDS 156..293
/label=ORF-I
/codon_start=156
-35_signal 1343..1367
-10_signal 1367..1372
misc_feature 1389..1408
/note="SOS Box"
/partial
CDS 1423..2238
/product="kanamycin resistance"
/codon_start=1423
BASE COUNT 541 a 535 c 548 g 638 t
ORIGIN
1 aacccattgg caaagtattt cttttggcgt catataaatt acctccagac ctgtaaataa
61 tccttttcca tagcataaca tcaaaatcct atcaagctca aaatttattg gcacgctcga
121 tgccatgttc ttcgactacg aagcggccaa agattatgcg acccgcttca ggctggtcac
181 ggcggcgaat atcccttctt tcggagcgcc agaatgatgt tggtctggcc ggattttcac
241 atggcgggaa ccacgttgtg tcggctttgt tgaataaatc agatttcggg taagtctccc
301 ccgtagcggg ttgtgttttc aggcaatacg cacgctttca ggcatacctg ctttcgtcat
361 tttgttcagc gctcgtacca gggccatagc ctccgcaacc tgaccatcgt agtcacgcag
421 cgtcagtgaa cccccgaaca gctgttttac ccggtacatc gccgtttccg ctatcgagcg
481 acggttgtaa tctgttgtcc atttccaccg cgcattactc ccggtcattc gctgattagc
541 cactgcacgg ttacggtctg catattcacc gggccagtaa cctgcacctt ttcggggagg
601 gataagcgcg ctgattttct tacgccgcag ttcatcgtga cagagccggg tgtcgtaagc
661 gccgtctgcc gctgctgccc tgatttttct gtgagtctgc cggataagac ccgggaaggc
721 ttctgagtcc gtcacattgt tcagcgacag gtctgcacag atgatttcat gtgtgttgct
781 gtcaacggcc agatgcaact ttcgccatat acgacggcgt tcctggccat gctttttgac
841 tttccactcg ccttcaccga agaccttcag cccggtggaa tcaattacca ggtgtgcgat
901 ttcaccccgg gtgggcgttt tgaaactgac attaaccgac tttgcccgcc tgctgacaca
961 gctgtaatcc gggcagcgta gcggaacgtt catcagagaa aaaatggaat caataaagcc
1021 ctgcgcagcg cgcagggtca gcctgaatac gcgtttaatg accagcacag tcgtgatggc
1081 aaggtcagaa tagcgctgag gtctgcctcg tgaagaaggt gttgctgact cataccaggc
1141 ctgaatagct tcatcatcca gccagaaagt tatggagcca cggttgatga gggctttatt
1201 gtaggtgggc cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc
1261 gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagccc
1321 gttgtgtctc aaaatctctg atgttacatt gaacaagata aaagtatatc atcatgaaca
1381 ataaaactgt ctgcttacat aaacagtaat acaaggagcg ttatgagcca tattcaacgg
1441 gagacgtctt gctcgaggcc gcgattaaat tccaacctgg atgctgattt atatgggtat
1501 agatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
1561 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
1621 gatgagatgg tcagactaaa ctggctgacg gcatttatgc ctcttccgac catcaagcat
1681 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca
1741 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagcg
1801 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
1861 tttcgtctca ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
1921 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg
1981 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
2041 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
2101 caggatcttg ccatcctatg gaactgcctc ggtgaatttt caccttcatt acagaaacgg
2161 tttttttata aatatggcat tgataatcct gatatgaata aattgcagtt tcatttgatg
2221 ctcgatgagt ttttctgata gctagtcttt ggtttccctg tc
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