GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS ASGBPHA 2360 bp ds-DNA PLN 17-MAY-1991
DEFINITION Aspergillus niger bphA gene for benzoate-para-hydroxylase (EC
1.14.13.12)
ACCESSION X52521
KEYWORDS benzoate-para-hydroxylase; bphA gene.
SOURCE Aspergillus niger DNA.
ORGANISM Aspergillus niger
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Plectomycetes; Eurotiales; Trichocomaceae.
REFERENCE 1 (bases 1 to 2360)
AUTHORS Van,G.R.F.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2360)
AUTHORS Van,G.R.F., Boschloo,J.G., Kuijvenhoven,A., Lange,J., Van,V.A.J.,
Bos,C.J., Van,B.J.A., Pouwels,P.H. and van,d.H.C.A.
TITLE Isolation and molecular characterisation of the benzoate-para-
hydroxylase gene (bphA) of Aspergillus niger: A member of a new
gene family of the cytochrome P450 superfamily.
JOURNAL Mol. Gen. Genet. 223, 192-197 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17549; BPHA$ASPNI.
*source: strain=NV DSM 2061 (ATCC 1015); clone=16.1 (pAB8-1);
*source: library=pKBY2; **map: chromosome=chromosome I;
From EMBL 26 entry ANBPHA; dated 19-OCT-1990.
FEATURES Location/Qualifiers
precursor_RNA 22..2337
/note="primary transcript"
mRNA 23..176
/note="exon 1"
CDS 28..81
/note="small ORF 1 (18 AA)"
/codon_start=28
CDS 71..85
/note="small ORF 2 (5 AA)"
/codon_start=71
intron 177..280
/note="intron I"
mRNA 281..608
/note="exon 2"
CDS 310..608
/note="benzoate-para-hydroxylase (AA 1-100) (608 is 2nd
base in codon)"
/codon_start=310
intron 609..688
/note="intron II"
mRNA 689..2337
/note="exon 3"
CDS 689..1940
/note="benzoate-para-hydroxylase (AA 101-517) (689 is 3rd
base in codon)"
/codon_start=689
misc_feature 2343..2343
/note="alt. transcriptional stop"
misc_feature 2350..2350
/note="alt. transcriptional stop"
BASE COUNT 515 a 735 c 532 g 578 t
ORIGIN
1 ctgcacttca actcaaaaaa aagggagatg acagatcaac cactttctcg acaagaaaca
61 ctcttcaccc atggcgatac ttgagtgaag taagtgtgac tgtgactgat attattttct
121 tcttcagcca tcccttttcg atatattacc ttatagttgc ttatttcatt tcggaggtaa
181 gatggtctcc ttggcaccca cattcccggc tgcacttggc ggctgcggaa ttgcttctcc
241 tggctgcatt tgccgatgtt aacttccgaa ctctcaccag cttcctcccc tccccccaca
301 aaaacaatca tgctcgccct gctcctgtca ccttacgggg cctatctggg tctagccctg
361 ctagtcctct actatctcct tccttaccta aagcgcgctc acctccgcga tatccccgcc
421 cccggtctgg ccgccttcac caacttctgg ctgctcctgc aaactcgtcg cggtcaccgt
481 tttgtcgtcg tcgacaacgc tcacaagaaa tatggaaagc tcgttcgcat cgcccctcgt
541 cacacctcca tcgccgatga tggcgccatc caggctgtct acggccacgg aaatggcttc
601 ctgaagtcgt acgtgcccct cgccgcttgt atttatctgc tcctggcagg tagaattatc
661 ctccggaagc taatcaactc ttgttcagtg acttctacga tgccttcgtc tccattcacc
721 gtggtctctt caacacgcgc gaccgcgcgg agcatacccg caagcgcaag accgtgtctc
781 acaccttcag tatgaagtcc atcggtcagt tcgagcagta catccacggc aacatcgagc
841 tctttgtcaa gcaatggaac cgcatggccg atacccagcg caaccccaag actgggttcg
901 ccagcctgga cgccctgaac tggttcaact acttggcttt tgacatcatc ggtgacttgg
961 cctttggcgc tcccttcggc atgcttgaca agggcaagga cttcgccgag atgcgcaaga
1021 ctcccgactc acctccttcc tacgtccaag ccgtcgaggt cctcaaccgc cgcggtgaag
1081 tctccgccac cctgggctgc tacccggcct tgaagccctt tgccaagtat ctccccgaca
1141 gtttcttccg tgatggtatc caagccgtcg aggacctggc tggtattgcc gtcgcccgcg
1201 tcaacgaacg tctccgcccc gaagtcatgg ccaacaacac ccgtgtcgac ctgctcgccc
1261 gtctcatgga aggcaaggat agcaacggcg agaagctggg tcgcgccgag ctcaccgccg
1321 aggcattgac ccaactcatc gccggatccg acaccacctc caacacctcc tgcgccatcc
1381 tctactggtg catgcgcacc cccggtgtga tcgagaaact ccacaaggcc ctggacgaag
1441 ccatccctca agacgtcgac gtccctaccc acgccatggt caaggatatc ccctacctcc
1501 aatgggtcat ctgggaaacc atgcgtatcc acagcacctc cgccatgggt ctcccccgtg
1561 aaatccccgc cggtaacccc cccgtcacca tctccggcca caccttctac cccggcgacg
1621 ttgtctccgt gcccagctac accatccacc gctccaagga gatctggggt cccgatgccg
1681 agcaattcgt ccctgagcgc tgggaccccg cccgccttac cccccgtcag aaggccgctt
1741 tcattccctt cagcacgggc cccagagcct gtgtcggtcg caacgtcgcg gagatggaac
1801 tcctggttat ctgcggtacg gtgttccgcc tcttcgagtt cgagatgcag caggagggtc
1861 ctatggagac gagagagggt ttcttgagga agcccctcgg actgcaggtc ggtatgaaga
1921 ggagacagcc tggttcggct tgaatcaggg gctgtggtgt atctcctaag gtttgagata
1981 ctcggaggaa atgaaagaag gaatgaacct ctgtgcatag aatttttccc ctacagaccg
2041 ggaagacgat tgaagagctt ttcctgtata tgtataagcg agttgcgaga atggattagt
2101 atatagttag cattcatcca tgcaatgaat gacatgatga gatgatcaat tcattatatt
2161 gacattttct tagattattt ttcttttctt ttccttttta tttgtccctc cgacattcga
2221 tgatatgata caatttgtta tagatttaca tgatcatcac atgtctgagg ataatttcct
2281 aattaggaac atcgtcatca tcaagatcca atatgcaagg caaaatatgt cacaggattc
2341 acattatgaa ttattttaat
//