GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS ASGGA01 2602 bp ds-DNA PLN 17-MAY-1991
DEFINITION A.niger glucoamylase genes, G1 and G2.
ACCESSION X00712 K02466
KEYWORDS alternate splicing; amylase; glucoamylase.
SOURCE Aspergillus niger DNA.
ORGANISM Aspergillus niger
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Plectomycetes; Eurotiales; Trichocomaceae.
REFERENCE 1 (bases 211 to 2563)
AUTHORS Boel,E., Hjort,I., Svensson,B., Norris,F., Norris,K.E. and
Fiil,N.P.
TITLE Glucoamylases G1 and G2 from Aspergillus niger are synthesized from
two different but closely related mRNAs
JOURNAL EMBO J. 3, 1097-1102 (1984)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2602)
AUTHORS Boel,E., Hansen,M.T., Hjort,I., Hoegh,I. and Fiil,N.P.
TITLE Two different types of intervening sequences in the glycoamylase
gene from Aspergillus niger
JOURNAL EMBO J. 3, 1581-1585 (1984)
STANDARD full automatic
COMMENT SWISS-PROT; P04064; AMYG$ASPNG.
[1] mRNA, exons only. Two forms of glucoamylase protein (G1 and G2)
are derived from the same gene through alternate splicing. They are
enzymatically and immunologically related, and have identical
N-terminal amino acid sequences, but differ in the COOH-terminal
region. A 169 bp intron (intron E) is spliced from the large mRNA
to generate a smaller mRNA which encodes the G2 protein. Promoter
sequences were found at positions 98-105 (CAAT), and 161-167 (TATA)
. This G1 sequence shows significant homology to the G1 sequence of
A.awamori (see separate entry).
From EMBL 26 entry ANGA01; dated 22-JAN-1991.
FEATURES Location/Qualifiers
mat_peptide join(:342..483,:559..845,:901..997,:1059..1696,
:1755..2438)
/product="glucoamylase G1 mature peptide"
mat_peptide join(:342..483,:559..845,:901..997,:1059..1696,
:1755..2097,:2267..2289)
/product="glucoamylase G2 mature peptide"
CDS join(:270..483,:559..845,:901..997,:1059..1696,
:1755..2441)
/product="preproglucoamylase G1"
/codon_start=270
CDS join(:270..483,:559..845,:901..997,:1059..1696,
:1755..2097,:2267..2292)
/product="preproglucoamylase G2"
/codon_start=270
prim_transcript 202..2563
/note="G1, G2 mRNA (alt.)"
prim_transcript 226..2563
/note="G1, G2 mRNA (alt.)"
sig_peptide 270..323
/note="glucoamylase G1 signal peptide"
sig_peptide 270..323
/note="glucoamylase G2 signal peptide"
intron 484..558
/note="G1, G2 intron A"
intron 846..900
/note="G1, G2 intron B"
intron 998..1058
/note="G1, G2 intron C"
intron 1697..1754
/note="G1, G2 intron D"
intron 2098..2266
/note="G2 intron E"
BASE COUNT 543 a 724 c 702 g 633 t
ORIGIN
1 ttcgtcgcct aatgtctcgt ccgttcacaa actgaagagc ttgaagtggc gagatgtctc
61 tgcaggaatt caagctagat gctaagcgat attgcatggc aatatgtgtt gatgcatgtg
121 cttcttcctt cagcttcccc tcgtgcgagt gaggtttggc tataaattga agtggttggt
181 cggggttccg tgaggggctg aagtgcttcc tcccttttag gcgcaactga gagcctgagc
241 ttcatcccca gcatcattac acctcagcaa tgtcgttccg atctctactc gccctgagcg
301 gcctcgtctg cacagggttg gcaaatgtga tttccaagcg cgcgaccttg gattcatggt
361 tgagcaacga agcgaccgtg gctcgtactg ccatcctgaa taacatcggg gcggacggtg
421 cttgggtgtc gggcgcggac tctggcattg tcgttgctag tcccagcacg gataacccgg
481 actgtatgtt tcgagctcag atttagtatg agtgtgtcat tgattgattg atgctgactg
541 gcgtgtcgtt tgttgtagac ttctacacct ggactcgcga ctctggtctc gtcctcaaga
601 ccctcgtcga tctcttccga aatggagata ccagtctcct ctccaccatt gagaactaca
661 tctccgccca ggcaattgtc cagggtatca gtaacccctc tggtgatctg tccagcggcg
721 ctggtctcgg tgaacccaag ttcaatgtcg atgagactgc ctacactggt tcttggggac
781 ggccgcagcg agatggtccg gctctgagag caactgctat gatcggcttc gggcagtggc
841 tgcttgtatg ttctccaccc ccttgcgtct gatctgtgac atatgtagct gactggtcag
901 gacaatggct acaccagcac cgcaacggac attgtttggc ccctcgttag gaacgacctg
961 tcgtatgtgg ctcaatactg gaaccagaca ggatatggtg tgtttgtttt attttaaatt
1021 tccaaagatg cgccagcaga gctaacccgc gatcgcagat ctctgggaag aagtcaatgg
1081 ctcgtctttc tttacgattg ctgtgcaaca ccgcgccctt gtcgaaggta gtgccttcgc
1141 gacggccgtc ggctcgtcct gctcctggtg tgattctcag gcacccgaaa ttctctgcta
1201 cctgcagtcc ttctggaccg gcagcttcat tctggccaac ttcgatagca gccgttccgg
1261 caaggacgca aacaccctcc tgggaagcat ccacaccttt gatcctgagg ccgcatgcga
1321 cgactccacc ttccagccct gctccccgcg cgcgctcgcc aaccacaagg aggttgtaga
1381 ctctttccgc tcaatctata ccctcaacga tggtctcagt gacagcgagg ctgttgcggt
1441 gggtcggtac cctgaggaca cgtactacaa cggcaacccg tggttcctgt gcaccttggc
1501 tgccgcagag cagttgtacg atgctctata ccagtgggac aagcaggggt cgttggaggt
1561 cacagatgtg tcgctggact tcttcaaggc actgtacagc gatgctgcta ctggcaccta
1621 ctcttcgtcc agttcgactt atagtagcat tgtagatgcc gtgaagactt tcgccgatgg
1681 cttcgtctct attgtggtaa gtctacgcta gacaagcgct catgttgaca gagggtgcgt
1741 actaacagaa gtaggaaact cacgccgcaa gcaacggctc catgtccgag caatacgaca
1801 agtctgatgg cgagcagctt tccgctcgcg acctgacctg gtcttatgct gctctgctga
1861 ccgccaacaa ccgtcgtaac tccgtcgtgc ctgcttcttg gggcgagacc tctgccagca
1921 gcgtgcccgg cacctgtgcg gccacatctg ccattggtac ctacagcagt gtgactgtca
1981 cctcgtggcc gagtatcgtg gctactggcg gcaccactac gacggctacc cccactggat
2041 ccggcagcgt gacctcgacc agcaagacca ccgcgactgc tagcaagacc agcaccagta
2101 cgtcatcaac ctcctgtacc actcccaccg ccgtggctgt gactttcgat ctgacagcta
2161 ccaccaccta cggcgagaac atctacctgg tcggatcgat ctctcagctg ggtgactggg
2221 aaaccagcga cggcatagct ctgagtgctg acaagtacac ttccagcgac ccgctctggt
2281 atgtcactgt gactctgccg gctggtgagt cgtttgagta caagtttatc cgcattgaga
2341 gcgatgactc cgtggagtgg gagagtgatc ccaaccgaga atacaccgtt cctcaggcgt
2401 gcggaacgtc gaccgcgacg gtgactgaca cctggcggtg acaatcaatc catttcgcta
2461 tagttaaagg atggggatga gggcaattgg ttatatgatc atgtatgtag tgggtgtgca
2521 taatagtagt gaaatggaag ccaagtcatg tgattgtaat cgaccgacgg aattgaggat
2581 atccggaaat acagacaccg gg
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