GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS ASGGLUA 2103 bp ss-mRNA PLN 17-MAY-1991
DEFINITION Aspergillus niger mRNA for glucoamylase G1 (1,4-alpha-D-glucan
glucohydrolase, EC 3.2.1.3)
ACCESSION X00548
KEYWORDS complementary DNA; glucoamylase; signal peptide.
SOURCE Aspergillus niger RNA.
ORGANISM Aspergillus niger
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Plectomycetes; Eurotiales; Trichocomaceae.
REFERENCE 1 (bases 1 to 2103)
AUTHORS Boel,E., Hjort,I., Svensson,B., Norris,F., Norris,K.E. and
Fiil,N.P.
TITLE Glucoamylases G1 and G2 from Aspergillus niger are synthesized from
two different but closely related mRNAs
JOURNAL EMBO J. 3, 1097-1102 (1984)
STANDARD full automatic
COMMENT SWISS-PROT; P04064; AMYG$ASPNG.
Data kindly reviewed (09-MAY-1985) by E. Boel
Removal of the 169 bp long intervening sequence from nucleotides
1639-1807 (aa 527 to aa 583) generates a G2 messenger RNA.
From EMBL 26 entry ANGLUA; dated 20-FEB-1985.
FEATURES Location/Qualifiers
CDS 60..113
/note="put. signal peptide"
/codon_start=60
CDS 114..131
/note="propeptide"
/codon_start=114
CDS 132..1979
/note="glucoamylase G1"
/codon_start=132
misc_feature 1639..1807
/note="intervening sequence"
BASE COUNT 432 a 625 c 562 g 484 t
ORIGIN
1 tcccttttag gcgcaactga gagcctgagc ttcatcccca gcatcattac acctcagcaa
61 tgtcgttccg atctctactc gccctgagcg gcctcgtctg cacagggttg gcaaatgtga
121 tttccaagcg cgcgaccttg gattcatggt tgagcaacga agcgaccgtg gctcgtactg
181 ccatcctgaa taacatcggg gcggacggtg cttgggtgtc gggcgcggac tctggcattg
241 tcgttgctag tcccagcacg gataacccgg actacttcta cacctggact cgcgactctg
301 gtctcgtcct caagaccctc gtcgatctct tccgaaatgg agataccagt ctcctctcca
361 ccattgagaa ctacatctcc gcccaggcaa ttgtccaggg tatcagtaac ccctctggtg
421 atctgtccag cggcgctggt ctcggtgaac ccaagttcaa tgtcgatgag actgcctaca
481 ctggttcttg gggacggccg cagcgagatg gtccggctct gagagcaact gctatgatcg
541 gcttcgggca gtggctgctt gacaatggct acaccagcac cgcaacggac attgtttggc
601 ccctcgttag gaacgacctg tcgtatgtgg ctcaatactg gaaccagaca ggatatgatc
661 tctgggaaga agtcaatggc tcgtctttct ttacgattgc tgtgcaacac cgcgcccttg
721 tcgaaggtag tgccttcgcg acggccgtcg gctcgtcctg ctcctggtgt gattctcagg
781 cacccgaaat tctctgctac ctgcagtcct tctggaccgg cagcttcatt ctggccaact
841 tcgatagcag ccgttccggc aaggacgcaa acaccctcct gggaagcatc cacacctttg
901 atcctgaggc cgcatgcgac gactccacct tccagccctg ctccccgcgc gcgctcgcca
961 accacaagga ggttgtagac tctttccgct caatctatac cctcaacgat ggtctcagtg
1021 acagcgaggc tgttgcggtg ggtcggtacc ctgaggacac gtactacaac ggcaacccgt
1081 ggttcctgtg caccttggct gccgcagagc agttgtacga tgctctatac cagtgggaca
1141 agcaggggtc gttggaggtc acagatgtgt cgctggactt cttcaaggca ctgtacagcg
1201 atgctgctac tggcacctac tcttcgtcca gttcgactta tagtagcatt gtagatgccg
1261 tgaagacttt cgccgatggc ttcgtctcta ttgtggaaac tcacgccgca agcaacggct
1321 ccatgtccga gcaatacgac aagtctgatg gcgagcagct ttccgctcgc gacctgacct
1381 ggtcttatgc tgctctgctg accgccaaca accgtcgtaa ctccgtcgtg cctgcttctt
1441 ggggcgagac ctctgccagc agcgtgcccg gcacctgtgc ggccacatct gccattggta
1501 cctacagcag tgtgactgtc acctcgtggc cgagtatcgt ggctactggc ggcaccacta
1561 cgacggctac ccccactgga tccggcagcg tgacctcgac cagcaagacc accgcgactg
1621 ctagcaagac cagcaccagt acgtcatcaa cctcctgtac cactcccacc gccgtggctg
1681 tgactttcga tctgacagct accaccacct acggcgagaa catctacctg gtcggatcga
1741 tctctcagct gggtgactgg gaaaccagcg acggcatagc tctgagtgct gacaagtaca
1801 cttccagcga cccgctctgg tatgtcactg tgactctgcc ggctggtgag tcgtttgagt
1861 acaagtttat ccgcattgag agcgatgact ccgtggagtg ggagagtgat cccaaccgag
1921 aatacaccgt tcctcaggcg tgcggaacgt cgaccgcgac ggtgactgac acctggcggt
1981 gacaatcaat ccatttcgct atagttaaag gatggggatg agggcaattg gttatatgat
2041 catgtatgta gtgggtgtgc ataatagtag tgaaatggaa gccaagtcat gtgattgtaa
2101 tcg
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