GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS ASNPENDE 2361 bp ds-DNA PLN 17-MAY-1991
DEFINITION Aspergillus nidulans penDE gene for acyl-CoA: 6-amino penicillanic
acid acyltransferase (AAT)
ACCESSION X53310
KEYWORDS acyl-CoA:6-amino penicillanic acid (6-APA) acyltransferase;
acyltransferase.
SOURCE Aspergillus nidulans DNA.
ORGANISM Aspergillus nidulans
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Plectomycetes; Eurotiales; Trichocomaceae.
REFERENCE 1 (bases 1 to 2361)
AUTHORS Montenegro,E.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2361)
AUTHORS Montenegro,E., Barredo,J.L., Gutierrez,S., Diez,B., Alvarez,E. and
Martin,J.F.
TITLE Cloning, characterization of the acyl-CoA:6-amino penicillanic acid
acyltransferase gene of Aspergillus nidulans and linkage to the
isopenicillin N synthase gene
JOURNAL Mol. Gen. Genet. 221, 322-330 (1990)
STANDARD full automatic
COMMENT From EMBL 26 entry ANPENDE; dated 26-OCT-1990.
FEATURES Location/Qualifiers
mRNA 298..333
/note="exon 1"
CDS 298..333
/note="AAT"
/codon_start=298
intron 334..386
/note="intron I"
mRNA 387..561
/note="exon 2"
CDS 387..561
/note="AAT"
/codon_start=387
intron 562..616
/note="intron II"
mRNA 617..768
/note="exon 3"
CDS 617..768
/note="AAT"
/codon_start=617
intron 769..827
/note="intron III"
mRNA 828..1538
/note="exon 4"
CDS 828..1535
/note="AAT"
/codon_start=828
polyA_site 1651..1656
/note="polyA signal"
BASE COUNT 612 a 632 c 544 g 573 t
ORIGIN
1 ggatccacgg gttccgtctc agtagcggct gcgtacacga tcctaaccta aaagaaccac
61 aggccaagcc cacatgcata gcatgttgat ttccctggcc cggcgccggc cgaaccttgg
121 tctagggctt tgtgcgctct gtcgattccg tgtcaaaact ggaccaggca gcaggaacat
181 gtctgtattc tagatctcat cggtacttgc cgccaactca cccaaatcgc gatctcgaat
241 gcacctatct agctagccac cctgttcaat attttctact ggacgctccg aagaaaaatg
301 cttcacgtaa cttgccaagg taccccctcc gaagtaagtc catacccgaa tgtatatttg
361 cccatattta acgcctatac ttccagatcg gctatcacca tggctctgct gccaaaggcg
421 agattgcgaa agccattgac ttcgcaactg gcctcattca tggcaaaaca aaaaagacac
481 aggcggagct tgaacagctc ctcagggagt tggagcaggt gatgaaacag cgctggccga
541 gatactatga ggaaatctgc ggtatgttta cctacgttct ttacttgctg aatcaccccg
601 ttgacggaat tttcaggaat cgcaaagggt gcggaacgcg aagtatcgga gattgtcatg
661 ctcaacactc gtacggaatt cgcgtacggg ctcgtagaag cccgggacgg gtgcaccact
721 gtttactgca aaacccccaa tggagcgcta cagggccaga actgggacgt aggttattag
781 atccacggtc tgctatctat tttccttgct aaccgccatt ccggcagttc ttcaccgcaa
841 ccaaagaaaa cttgatccag ttaacaattt gtcagccggg tctacccact atcaaaatga
901 ttacagaagc tggtatcatt ggcaaagtgg gtttcaacag tgctggtgtc gctgtcaatt
961 acaatgcact acacctacat ggcctccgtc ccactggcct cccctcgcat ctcgcgctgc
1021 gcatggccct cgaaagtaca tctccgtctg aggcgtatga aaaaatcgtc tcgcaagggg
1081 gcatggcggc tagcgcgttc atcatggtgg gcaacgcaca cgaggcctac gggctagagt
1141 tctcgcccat cagcttgtgc aagcaagttg ctgacaccaa tgggcggata gtgcatacga
1201 accactgcct cctcaaccat gggccatcgg cgcaagagct taatcccctg ccggactcgt
1261 ggagccgcca cgggcggatg gaacatctcc tctctggttt tgacggcacg aaggaggcat
1321 ttgcgaagtt gtgggaggac gaagacaact accctctctc gatctgccgg gcatataagg
1381 aagggaaaag tagaggctcc actcttttca acatcgtctt cgatcatgtg ggccggaagg
1441 caacagtgcg gctgggccgg cccaataacc ctgatgagac ctttgtcatg acctttagca
1501 atctggatac caagtccgcg atccaagcca acatttgacc aatatctctt tccaaccgat
1561 gccttctgca gtcttctcca gccatagcgt actacaccgt gaacacttct atgtatcttg
1621 acaactagat acaagtcaaa aggcgaatca aataaagtcg catgtttggg agaatgcacg
1681 ccagagtgaa atgctgaatc ctgttggctt tcacaaccag gtgcctaaag tatagcaatg
1741 caacgaaggc atgcctagtc tagcttcttt ccctaaacat gctttaaatc taatacgaga
1801 attatttaaa gcattccaga actactacta acttcctatt accctggagt ttcctgtttc
1861 ggaagggttg acctctcaat ccttcctaac cttgtgagtc tagagtaagc tctgcaatct
1921 tgtttaacct acctaggact cacgaagact ccctcagtat gaagttgata aaggttcttt
1981 gtgctccgag cccgcaaggg aaatcagtgc tgagatatac tggctgggct ctatatgttg
2041 tagagtcaag tgttgacaat ttgaatcctc gctaatgtca cggctctgca agccgggatc
2101 ccgagttggt aaagcatgca acagcaagcc ccattttatc tacatattag ctacgattat
2161 cctaatcatt gcttttagaa taatctctaa atttggagcc agcaggtcac agagctcact
2221 ctaggcaccc cgggcacagt gctcgaaata gcgtcgtgcc gttatccagc ttcatacatt
2281 ggtgtaaaag aagaatcgag ccatttctgg ccacccatct gataagttag gcctagctcc
2341 gtgaagacat tccacaaaat c
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