GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS ASNYALAC 4337 bp ds-DNA PLN 17-MAY-1991
DEFINITION Aspergillus nidulans yA gene for laccase (EC 1.10.3.2)
ACCESSION X52552
KEYWORDS laccase; oxidoreductase; yA gene.
SOURCE Aspergillus nidulans DNA.
ORGANISM Aspergillus nidulans
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Plectomycetes; Eurotiales; Trichocomaceae.
REFERENCE 1 (bases 1 to 4337)
AUTHORS Aramayo,R.A.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 771 to 3211)
AUTHORS Aramayo,R.A. and Timberlake,W.E.
TITLE Sequence and molecular structure of the aspergillus nidulans yA
(Laccase I) gene.
JOURNAL Nucleic Acids Res. 18, 3415-3415 (1990)
STANDARD full automatic
COMMENT SWISS-PROT; P17489; LAC1$ASPNI.
*source: strain=Winter strain, FGSC4; clone=pRA1,pRA3-21. **map:
map position=linkage group I. Two cDNA clones also used for
sequencing-pRA7B11, pRA19A11. Look for 8 N-linked glycosylation
sites and 13 Cu-binding. subcellular localisation, p-Di-phenol:
oxygen:oxidoreductase. developmentally regulated gene, product=
oxygenase catalysing pigment synthesis.
From EMBL 26 entry ANYALAC; dated 01-AUG-1990.
FEATURES Location/Qualifiers
mRNA <913..1107
/note="exon 1"
misc_feature 775..801
/note="put. CAT box"
promoter 815..821
/note="put. TATA box"
CDS 913..1107
/note="laccase protein (AA 1-65)"
/codon_start=913
intron 1108..1161
/note="intron I"
mRNA 1162..1217
/note="exon 2"
CDS 1162..1217
/note="laccase protein (AA 66-84) (1217 is 2nd base in
codon)"
/codon_start=1162
intron 1218..1288
/note="intron II"
mRNA 1289..1459
/note="exon 3"
CDS 1289..1459
/note="laccase protein (AA 85-141) (1289 is 3rd base in
codon) (1459 is 2nd base in codon)"
/codon_start=1289
intron 1460..1534
/note="intron III"
mRNA 1535..1705
/note="exon 4"
CDS 1535..1705
/note="laccase protein (AA 142-198) (1535 is 3rd base in
codon) (1705 is 2nd base in codon)"
/codon_start=1535
intron 1706..1758
/note="intron IV"
mRNA 1759..1884
/note="exon 5"
CDS 1759..1884
/note="laccase protein (AA 199-240) (1759 is 3rd base in
codon) (1884 is 2nd base in codon)"
/codon_start=1759
intron 1885..1948
/note="intron V"
mRNA 1949..3198
/note="exon 6"
CDS 1949..3056
/note="laccase protein (AA 241-609) (1949 is 3rd base in
codon)"
/codon_start=1949
misc_feature 3108..3113
/note="put. polyA signal"
polyA_site 3198..3198
/note="polyA site"
BASE COUNT 1064 a 1115 c 1087 g 1071 t
ORIGIN
1 ctcgaggttc tccgcctagc tggtgggttg aaccgcttac tcagtgtcga aaggataaat
61 gagatcaatt tctcccgtga agccattcag caaggtatcc atgcggctct cgtggaacac
121 aatgctaacg ccctcaccac cctccttaag ctcgacgagt ttaccttccg ttgccagaac
181 aaccttgtag atgccgtgta taccctacca ccagagcatt tccgcaccgc tgtccgtgtg
241 gcacgacacg actctagctt cttccagctt ctgctgcgtg cgagtgctga gtctgttccc
301 tacgacgatt cggagatcac gcactgggca gttactacgg gtggtccttt tgaacaatgg
361 ctactggatt tcatgctgca gctgccggag caaatcaggg cgacaaccaa gggaagtcaa
421 caaatgttct atcatggggg cttgaataca gcaagtgcca tgacagaaag gtatcttcga
481 gatattctgg gaacggatga gcttaaggtc tggctagaag agtcgtcgta cgattttccc
541 agcgaatgga ttgttgagag ttgatgttga agatattgaa caaatttctg tggtgatgag
601 cgctggaaga atcagtgtac aacgaaagga gcagacaaga ctcccttgct atactcaggt
661 gtgacacctg caaatccagt aggaaaatac ggtaattaat cccagggtct tttccctccg
721 ccaggcacca cgccccttgc cggaaacgtc ggccagtcgc gttcagcacc aggtacattc
781 caatcaacaa tgcaagaatg tgaaacgcga agtatatatc agcgaacaac atcaagccga
841 cataatcaaa cggtttcagt ctctatcgcg ttttgcactt gagcgtcatt ttgattcctc
901 cgggatccag ccatgtacct ctccacggtc ctcttcccac tactggctct gaaccttggt
961 ctctcacacg cgagattcgt ccgtgaaact ctcgagctga catgggaata cggatcaccc
1021 aacggcggta cgccgcgcga gatggtcttc acgaatgggg agtatccagg tccggatctg
1081 atatttgatg aagatgatga tgtcgaggta caaccaagtt ttcaccgtat gtacatccga
1141 caacagagtt gacaatcaca ggttcttgtc atcaataacc ttcccttcaa tacaaccgtt
1201 cactggcatg gtttagagtg tgtgcccttt ccgcgtccca tttctgcatg gacctaaaaa
1261 ttcttgcatt ctcacacact ctctctagaa tgcgagaaac acctgaagcg gacggtgtcc
1321 cgggccttac gcaaacacca atagagcccg gtgcgacgtt cacgtatcgg ttcagagcgt
1381 atccagctgg tacgttctgg tatcactcgc attacaaggg gctcatgcaa gatgggcagg
1441 taggagcaat gtatattcgg tacgtctgat cagattacac cttatctgca cgatgaagcc
1501 cattgaggca gtcttattga tcatgtctca atagccgcaa gcctgatgct ttgcgaccct
1561 acgcggttat aacggaagac ccgagagagt tagcagaaat acagtacgca gaagataatc
1621 cttatttgat gcttgctacg gactggacat atcttacttc ggcggagtat cataacatcg
1681 aggttgagtc tggctataac gtcttgtaag ctcgattttt cctttacttc ctgttgcgaa
1741 ctaattcaat tgcaaaagct gcgtggatag tctcctcatc aatggtcgag gctcagtcta
1801 ctgtcccgga taccaatacc ttgaggaagt gagcgacacg gccttactgt ccgtcctcga
1861 aggaacacat ctgaccgaga aagggtaagt ctctgcacgc gtacgcccag tcaagattat
1921 ataaatactg atattgtatg atacatagct gcctccaacc ggacctacat aatgtccaag
1981 gcgactacgg cccctggaac ctctctgctg ttcccacaga agtcgtcttt aattgcaccc
2041 cgtccagtgt cgagcccccg gtgatctacg tcgatcccga attcaacggt tgggtcagcc
2101 ttaatttcat tggcggcgct gcgcaaaagg ccataacttt ttctgtcgac aatcacccaa
2161 tgtgggtata tgaagttgac ggacagtttg ttgaaccgcg tgaagtcgaa atggttggcg
2221 tatactcggg agctcggtac gccgtcatga tcaagctaga ccagacacca ggcgactacg
2281 cgattcggat tgccgtcaat ggcgcggatc aggttatgag cgtttacgcc atcctctcgt
2341 atgtcaatca ggattggatc cacagggaga atgtgcccaa ggccgctatc ggaccgcata
2401 cagatacagt gggctacatg aactacggcg gtggcaacac atctgcagat gtacgccagc
2461 ttctcttcac agaaaatctg ccggcctttg cggtcccgcc tcccccacct tcgagcgagg
2521 tctcaacaac cctacgtacc ggcatgatcc gcgtaaacaa ctcctactct tggtcattgg
2581 gaaacaacgt gctgtacgag cccgaaatga cctcctcgac accgctcctc ttcgaaccgg
2641 atccgctagc cgtcatcgca ccaaagtacg ccctcacgac agaaaacaac acctgggtcg
2701 acattgtcct cgaaatcact gccgacccgc gcgacctcat ccacccgcca catccgatcc
2761 ataagcacgg taacagagca tatataattg gcaacggggt tggcaaattc agatgggaga
2821 atgtgagtgc tgcagaggca gaagtaccgg atttgtttta cgtcaacgag actgcggcgt
2881 tgcgggatac atttgtcacg gatttttttg attcgcggct catggatggg gcatggatag
2941 ttattcggta ttttgtgcag gacaagttcc caagtatctt gcattgtcat attgcgtccc
3001 atcaaatggg aggaatggcg ctggccttgt tggacggggt tgatgtttgg gattcttagg
3061 tgagctctca tattcgtact tacaagatgt ttgttctaaa attatttaat aattattttt
3121 taatctctat gaaatggaga agcttttttt tctctctttt ttggcttctt taaattatat
3181 cagcatattt attaaatcat tggaactcta gatgtacgcc ccagagaagt agaactacat
3241 attcccaatc aacctcagaa tacctctgac cctcccagcc agcgtgatct gataccccgt
3301 ctcaacaacc cccttgcaca tctcaaatgc accatcccca ttctctttct ccgcttctcc
3361 cttcataatc ggataatcca cgcgcggcgg aatcctcgtc gccataaggc gcgggaacct
3421 gtcaacgcaa atcgctttga tattacttac gccccgcaga ccttcttcgc ccgcaaaccg
3481 gccataccca gatcccttga cgccgccgaa tggcagctgt actgtgtagt agctgccaaa
3541 atcgttaaca gagaccatgc ctgcctttat tcctgagaca caagcattca cgtcgcgcgt
3601 gttgtagcca aataccgagg cacctagcgc gtattgggta gaatttgcga tggtgattgc
3661 gtcggagacg gaagatgcac gcatcatgag gaagacgggg gcgaagagct ctgtttgggc
3721 aatttccatg gagggcgtga cgtctgcaag gagggtcggt gtgaaatagt gaccgagcgg
3781 ataggttggg tgttcgaatt gtttcccacc agcgacgagg cgagcacctt ggctgacggc
3841 gcggctgaat gagaaactcc aagcgggaga aagaggccgg ggagatcatg gcccccacgt
3901 ctggggcacc tgacttgttg tttgggttgt tgggctttgt gtctagtaag actgagaccg
3961 aggcggaggg ctttaatgcg ggaggtgacg gtgtcaagga gtttgtgtat acgccaggga
4021 gggcaatgac gcgctccaac ccgatgcagt tttgaccggc ggattggaaa acgccgcgca
4081 tgaggacaga tgcgacagag gttacttcgc tgatagttct gctgtcatcg aggatgacag
4141 agggatcttt cccaccgagt tcgactgtta ctggtgtcaa tgcctttgct gcagactcgc
4201 atactttgtg cgccaccgga cgcgagccga tgaatgtgat ctgggcaatt ccagggtgcg
4261 aggttaaatg gtcggcgacc ttaggcagac agacaacgct ctgaacgaga tcacggggat
4321 ggccacagtt ttctaga
//