GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS ASOALPG 1820 bp ds-DNA PLN 17-MAY-1991
DEFINITION A. oryzae Alp gene for alkakine protease (EC 3.4.21.14)
ACCESSION X54726
KEYWORDS alkaline protease; extracellular enzyme; protein-degrading enzyme.
SOURCE Aspergillus oryzae DNA.
ORGANISM Aspergillus oryzae
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Plectomycetes; Eurotiales; Trichocomaceae.
REFERENCE 1 (bases 1 to 1820)
AUTHORS Cheevadhanarak,S.
JOURNAL Unpublished (1990)
STANDARD full automatic
COMMENT *source: strain=U 212; *source: library=lambda EMBL 3;
Data kindly reviewed (07-JAN-1991) by Cheevadhanarak S.
From EMBL 26 entry AOALPG; dated 15-JAN-1991.
FEATURES Location/Qualifiers
mat_peptide join(:552..957,:1017..1105,:1162..1512)
/product="alkaline protease"
/gene="ALP gene"
mRNA join(:<90..462,:513..957,:1017..1105,:1162..1512)
/gene="ALP gene"
exon <90..462
/gene="ALP gene"
CDS join(:<90..462,:513..957,:1017..1105,:1162..1512)
/product="preproalkaline protease"
/gene="ALP gene"
/codon_start=90
TATA_signal 35..40
prim_transcript 90..1720
/gene="ALP gene"
sig_peptide 139..201
intron 463..512
/number=1
exon 513..957
/gene="ALP gene"
intron 958..1016
/number=2
exon 1017..1105
/gene="ALP gene"
intron 1106..1161
/number=3
exon 1162..1720
/number=4
/gene="ALP gene"
BASE COUNT 427 a 512 c 442 g 439 t
ORIGIN
1 ctcgagaaca gggaaatata gaactcctga aggttataaa taccacatgc atccctcgtc
61 catcctcact tccatcatca agccagcggt ttctatccga cttgagttgt tcttgcgcat
121 ctttacaatc ttctcatcat gcagtccatc aagcgtacct tgctcctcct cggagctatc
181 cttcccgcgg tcctcggtgc ccctgtgcag gaaacccgcc gggccgctga gaagcttcct
241 ggaaagtaca ttgtcacatt caagcccggc attgacgagg caaagattca ggagcatacc
301 acctgggcta ccaacattca ccagcgcagt ctggagcgtc gtggcgccac tggcggtgat
361 cttcctgtcg gtattgagcg caactacaag atcaacaagt tcgccgccta tgcaggctct
421 ttcgacgatg ctaccattga ggagattcgc aagaacgaag atgtttgtgg tcatccgctc
481 gcatttttga atgacagcta actcgcgccc aggttgccta cgtcgaggag gaccagatct
541 actacctcga tggcctgact acccagaaga gtgccccctg gggtctgggc agcatttccc
601 acaagggcca gcagagcacc gactacatct acgacactag tgccggcgag ggcacctatg
661 cctacgtggt ggatagcggt gtcaatgtcg accatgagga gttcgagggc cgcgccagca
721 aggcctacaa cgctgccggt ggtcagcatg tggacagcat tggccatggc acccacgttt
781 ccggcaccat tgctggcaag acttatggta tcgccaagaa ggccagcatc ctttcggtca
841 aagttttcca gggtgaatcg agcagcactt cgctcattct tgacggcttc aactgggctg
901 ccaacgacat tgttagcaag aagcgtacca gcaaggctgc aatcaacatg agcttgggtg
961 agtttacatt gttcttctct acttggaatc gcgagtcgct aatttcaaaa acacaggcgg
1021 tggctactct aaggctttca acgatgcggt cgagaacgca ttcgagcagg gtgttctctc
1081 ggttgtcgct gccggtaacg agaacgtacg tctcccctcc atcgcgcaaa gacgaattcg
1141 taactgactt gattttctta gtctgatgcc ggccaaacca gccctgcctc tgcccctgat
1201 gccatcactg ttgccgctat ccagaagagc aacaaccgcg ccagtttctc caactttggc
1261 aaggtcgttg acgtcttcgc tcccggtcaa gatatccttt ctgcctggat tggctcttcc
1321 tctgccacca acaccatctc tggtacctcc atggctactc cccacattgt cggcctgtcc
1381 ctctacctcg ctgcccttga gaacctcgat ggccccgctg ccgtgaccaa gcgcatcaag
1441 gagttggcca ccaaggacgt cgtcaaggat gttaagggca gccctaacct gcttgcctac
1501 aacggtaacg cttaagtacc aggagtacgt cgcaggattc taccattgtt actggaatac
1561 aatgatgatt agaaaacgaa gagcgttatg attcggacgg atatatgcat ggcacccata
1621 cagcgtgata cataggctgt ttgctcaaga attaggattt tatctgaatc catgtacaga
1681 gtatacttat gttagtagtc aataaaatct tggctttcta attttgtccg atctacaagg
1741 ggtcgtcgat cacagaacga actagatgtg caggggacga tgatcacccg ttcttagcaa
1801 gacctctagt agttgtcgac
//