GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS NEUCYT22 1595 bp ds-DNA PLN 17-MAY-1991
DEFINITION Neurospora crassa DNA for cyt-21 gene encoding a mitochondrial
ribosomal protein S-24
ACCESSION X06360 J03533
KEYWORDS cyt-21 gene; ribosomal protein; ribosomal protein S24.
SOURCE Neurospora crassa DNA.
ORGANISM Neurospora crassa
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Pyrenomycetes; Sordariales; Sordariaceae.
REFERENCE 1 (bases 1 to 1595)
AUTHORS de,V.H.
JOURNAL Unpublished (1987)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Kuiper,M.T., Akins,R.A., Holtrop,M., de,V.H. and Lambowitz,A.M.
TITLE Isolation and analysis of the Neurospora Crassa CYT-21 gene: a
nuclear gene encoding an mitochondrial ribosomal protein
JOURNAL J. Biol. Chem. 263, 2840-2847 (1988)
STANDARD full staff_review
COMMENT SWISS-PROT; P08580; RS24$NEUCR.
*source: strain=wild-type 74A; clone=pUCK; In mutant cyt 21-1 a g-a
transition at pos. 637 prevents splicing of the first exon at the
regular site. Instead, 15 bp downstream a splice site is recruited.
The name of the encoded protein is provisional.
From EMBL 26 entry NCCYT21; dated 11-MAY-1990.
FEATURES Location/Qualifiers
misc_feature 294..298
/note="put. CCAAT-box"
misc_feature 325..332
/note="sequence homologous to cyt-22 gene"
precursor_RNA 349..1234
/note="put. primary transcript"
mRNA 349..564
/note="Exon 1"
misc_feature 426..433
/note="sequence homologous to cyt-22 gene"
misc_feature 454..462
/note="sequence homologous to cyt-22 gene"
CDS 488..564
/note="cyt-21 gene product (AA 1 - 26) (564 is 2nd base in
codon)"
/codon_start=488
intron 565..637
/note="Intron I"
mutation 637..637
/note="g is a in cyt 21-1 mutant effecting mRNA splicing"
mRNA 638..818
/note="Exon 2"
CDS 638..818
/note="cyt-21 gene product (AA 27 - 86) (638 is 3rd base
in codon)"
/codon_start=638
intron 819..917
/note="Intron II"
mRNA 918..1234
/note="Exon 3"
CDS 918..980
/note="cyt-21 gene product (AA 87 - 107)"
/codon_start=918
BASE COUNT 411 a 433 c 396 g 355 t
ORIGIN
1 gaattccgtt cagtttgtcc tacagtacag tagaggtact aattgctccc ctttgaattt
61 tgtgattgcg tgtacagtac gctgcagtac aatagtgtgc tactgtactg acatcactcg
121 ctgctccagt tcctccattg acggggatcg tcgatccagt gcataggtca tgatcccact
181 cgtgcggtcg tacctgctac catcaaaatg cgtggctgag acttacgttt gaatgtggct
241 tcaataaaaa aagtgtggct catgacggaa gcagcgaact ccgacataac cagccaattg
301 cctgccggga caccacaaag cttctgagcc ggcactgcag caccccggcc cacaatcttt
361 cagtgagcga tccggcaatc gggcaacgcg acgacgtctg gtccgcgcaa aagattcagt
421 cgacatctcg aacctcgtcg gcgacaattc ctccaccacc gcatctatca acctcccgcg
481 gtccaacatg gttctcaagc tcagactcgc ccgctttggg cgtaccaatg ccccctttta
541 caacattgtc gttgcccacg ccaggtacgc aattatccca tcacaacgac acgaccaggc
601 agacagccac cagctaactc tctgactccc atattagaac cgcgcgcaac agcaagcctc
661 tcgaggtcat tggcacatac gaccccgttc ccaagaagga cacgtacgat accacaggga
721 agctgcacaa ggacatcaag ctggacgtca cgagggccaa gtactggata ggtgtgggag
781 ctcagccgac cgacacggtt tggcggttac tgtcccttgt acgtttctct ctctctctat
841 acacaccaaa acctttatag gcaggtcaac gtctcgaaac cgattgactg acattgcaat
901 ccctcccacg aaaacaggtc ggcatactag accccaagta caagaagccg caaccggcgc
961 aggagcaaaa gaccaaggct tgagcgaatt gcaccttttc gattatttcc atacgtacca
1021 ccggacaacc cgggtcatct cgcatacccg atcatctgac accatcacca tccgtccatc
1081 catccatcca tgtattacaa accggctttc ttccatctag gcgtttttct gttctctaca
1141 ctatctaggg gttttccaaa aaaaggggtt tgtacgatac ctactatagc tcgacatata
1201 tatatataag aagcaaagta tacaacaccc accgaacgac gcgactttaa ttgttgaata
1261 aatcccggag gcagctaata cttgaacgtg atgggagcgg aaggggcgac gctttttgaa
1321 ggatgggaga gggggagggg aggaggagga ggaggaggac acagagtcgg agttcagttt
1381 gatgaggttt ggtcggtggg ctggaatgtt gcatggaaga atgggggatg acttcatcag
1441 caggggcaga cattgtggtg tttgtgtccc cagggtcgat acatctgctt gcccgtagtt
1501 gttgggtggg cagaggggtg cgattcatgt tggaacatca gaaggctaca ggcgaaaagc
1561 catcgcaatc accaaggcag ctggacgttg gtacc
//