GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS PBLLEU1 3474 bp ds-DNA PLN 17-MAY-1991
DEFINITION Phycomyces blakesleeanus leu1 gene for alpha-isopropylmalate
isomerase
ACCESSION X53090
KEYWORDS alpha-isopropylmalate isomerase; isomerase.
SOURCE Phycomyces blakesleeanus DNA.
ORGANISM Phycomyces blakesleeanus
Eukaryota; Plantae; Thallobionta; Eumycota; Zygomycotina;
Zygomycetes; Mucorales; Mucoraceae.
REFERENCE 1 (bases 1 to 3474)
AUTHORS Iturriaga,E.A.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 3474)
AUTHORS Iturriaga,E.A., Diaz-Minguez,J.M., Benito,E.P., Alvarez,M.I. and
Eslava,A.P.
TITLE Nucleotide Sequence of the Phycomyces Blakesleeanus leu1 gene.
JOURNAL Nucleic Acids Res. 18, 4612-4612 (1990)
STANDARD full automatic
COMMENT *source: strain=NRRL1555;
From EMBL 26 entry PBLEU1; dated 15-AUG-1990.
FEATURES Location/Qualifiers
promoter 461..468
/note="pot. TATA box"
misc_feature 508..508
/note="transcriptional initiation site I"
misc_feature 674..674
/note="transcriptional initiation site II"
promoter 722..725
/note="pot. TATA box"
misc_feature 758..758
/note="transcriptional initiation site III"
CDS 813..2879
/note="alpha-isopropylmalate isomerase (AA 1-689)"
/codon_start=813
polyA_site 3127..3131
/note="polyA signal"
BASE COUNT 879 a 841 c 736 g 1018 t
ORIGIN
1 aagcttaata ttaacattct ttaatgactt gcaggcaact tgtgaccagt cttttggtcg
61 gctttttgat agctcctcga ggtgaccgta aacgatatga tatcttgaca attcttgcta
121 gtgtattaca attgagcacc gagcaaaagg aacagattgg attgatacga agcactggcc
181 tgccgcgctc atcttcgtct agttcagtta ccagctggca gaacccccgc cattcaatcc
241 acgaggatga acctaaagaa gtatggaatt tgtttttctg gttttcttgc tgcttatctt
301 ttaacatttc aatgcttatt ttgattttgt attttttcga tcagtctttt acagatgcat
361 ggatttcttt cctactcaag gaatcaaaca cacatgcccg ttcaaggcag gaatctcctg
421 aaatcactca agatatctaa aaaaattaat aaaatacaaa tatatataca ccacacacac
481 acaaacacga aaattatttg tctaatcatc ctagtggctc ttcactaggc agataacaca
541 gtcagagtca gactgaacgc tcgttggcta ggatattctg gcggaataaa aaaaagtaca
601 gcatgaatat gctgagcggt aaaacaatac attagtccat cttttgctga tcagccaatc
661 accgtacata tttcactgac tcacggggga tacatgccca accatgctaa aacttaacca
721 ctatatgaac cattgactcc ttcaactatt tagcaccaac acccccttct ctttcgctct
781 cttataactt tttttctctc tttatcttac taatgtcccg tacactctac gacaaagtct
841 gggatgacca tgtcatcgat agacatcttg ttcatgaagt caccagccct caagcctttg
901 aaggtcttcg taatgccagc agacgtgttc gtcgtcctga ctgtactctc gcaactgtgg
961 accacaacat tcccaccacc acacgcaaga agttcaagag catcactact tttattgacg
1021 aggctgattc gcgaacccag tgcgagactc tcgaaacaaa cgtcaaggag tttgagctga
1081 cctactttgg aatggaagac agtcgtcagg gtattgtcca cgttatcggt cccgagcagg
1141 gtttcacact tcccggcacc actgttgtgt gtggcgattc ccacacttcc actcacggag
1201 ccttcggtgc tcttgctttt ggtatcggta cttctgaggt cgagcacgtt ctggccaccc
1261 agaccctttt gcagaagaag tccaagaaca tgcgtgtttg cgttgaaggt gaattgacac
1321 ctggtgtcac tagcaaggat gtagctttgc atgttattgg tcttattggt accgcaggtg
1381 gaactcgatg cgttatcgag ttttgtggat ctgccattgc ctcgttgtct atggaagccc
1441 gtatgtcgat ctgcaacatg tctattgaag gtggcgctcg tgcaggcatg atcgcacctg
1501 atgagattac attcgagtac ctccgtggaa gacctcttgc tcccgaaggc gccgagtggg
1561 acaaggctgt tcagtactgg aaatccctca agtctgatcc caacgccaag tatgatattg
1621 atgttaagat tgcagcttca gatattgctc ccaccatcac ttggggaaca agtcctcaag
1681 atgtcgctcc catcactgcc aatgtccctg atccctccag tgtctcggat cccgctcgca
1741 aggctgctat ggaacgtgcc ctggaataca tcggcttggt tcccaacact cccttggaag
1801 aggttaagat cgacaaggca ttcatcggaa gttgcacaaa ctctcgtatt gaggatctcc
1861 gtagtgcagc cagcattgtc aagggtaaac atattgctga ctgggtctac gccatggttg
1921 ttcccggttc aggcttggtg aagcgtcaag cagagcgtga gggtttggat aaggtgttca
1981 ctgatgctgg ttttgattgg agagaggccg gttgttcgat gtgtctcggt atgaaccctg
2041 atcagttgag ccctggagag agatgtgcca gtaccagcaa ccgtaacttt gagggcagac
2101 aaggtgccgg tggccgtact catcttatga gccctgccat ggctgccgct gccgctattc
2161 gtggctacct tactgatgtc cgcaagttct cttccactcc catggttccc cgcagtcctc
2221 ctccaaagtt ccagaccatc cagccaaagg tcgaggatga agctgcccac aagcaagctg
2281 ccgaccaggc cgatcctgtc actgactgtc ctcctgctgg tagtcccgtc aacaagggtg
2341 ctcctgtcgc aagcgccatg cctgcgttta ccacacttaa gggtgttgct gctcctctgg
2401 ccatctccaa cgtcgacact gacatgatta ttcccaagca gttcctcaag accatcaagc
2461 gcactggtct cggcagcgct ttgttctatg gccttcgtta tgatcctgcc actggtgctg
2521 aaaagcctga ttttgttctc aatcaacccg cctaccgctc atcaaagatc ctggtctgta
2581 ctggtcccaa ctttggctgc ggtagttccc gcgagcatgc tccttgggct tttaacgatt
2641 ttggtattcg ctgtattatt gccacaagtt ttgccgacat tttcttcaac aactgcttta
2701 agaacggcat gttgccaatc attctttccc aggagcaggt cgataccctc gccaagtatg
2761 ccacacagaa ggctgagatt gaagttgatc ttgttcatca gaagattaga taccccggtg
2821 gtgagattcc ttttgatgat cgaggaattc cgcaagcatt gtttggtgaa tggtttggat
2881 gacattggtc ttactatgca gaaggatagc gcgattgaga agttcgaagc caagcgtact
2941 tctacctggc cctggttgga cggcaaggca tacaagggaa aggctaccaa ggtcactgct
3001 attggatcag cttctcagcc cgctaaaaag ttggattggt agaatccttc aatttatcat
3061 atatatatat aactctatat ataaaaaata tacttcatta tcctcttaat cctttccata
3121 ttaaacaata aatcaaatcc tgtaaatttc tgttcatatc ttacacaacc aagtctttct
3181 ttcctttttg agaaacaatg ttcttttcta tttgtgggga tgtgcgttta gtatacagaa
3241 attgtgcata tttgtggcga atatatatat atcataggag gcttggaaac tttgtattaa
3301 aagatgaatt ttccttttta gcttttatct ccacacttgc tcacctaata tatatagcca
3361 ataagctatg gctattactc cttattattt gctctttgaa atgatgagat ctatagattg
3421 aatatccttt aaataccatc attacaacat atactttgtt gtatttttaa agct
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