GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS YSCPHO35 3913 bp ds-DNA PLN 17-MAY-1991
DEFINITION Yeast genes PH05 and PH03 for repressible (PH05) and constitutive
(PH03) acid phosphatase (E.C.3.1.3.2)
ACCESSION X01080
KEYWORDS phosphatase; signal peptide; tandem repeat.
SOURCE Saccharomyces cerevisiae DNA.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 3913)
AUTHORS Bajwa,W., Meyhack,B., Rudolph,H., Schweingruber,A.-M. and Hinnen,A.
TITLE Structural analysis of the two tandemly repeated acid phosphatase
genes in yeast
JOURNAL Nucleic Acids Res. 12, 7721-7739 (1984)
STANDARD full automatic
COMMENT SWISS-PROT; P00635; PPA5$YEAST.
Data kindly reviewed (18-NOV-1985) by W.Bajwa
From EMBL 26 entry SCPHO35; dated 06-JUL-1989.
FEATURES Location/Qualifiers
misc_feature 493..493
/note="minor transcription initiation site (PH05)"
misc_feature 502..502
/note="minor transcription initiation site (PH05)"
misc_feature 504..504
/note="major transcription initiation site (PH05)"
misc_feature 522..522
/note="minor transcription initiation site (PH05)"
CDS 542..592
/note="signal peptide (aa -17 to -1)"
/codon_start=542
CDS 593..1942
/note="repressible acid phosphatase (PH05) (aa 1-450)"
/codon_start=593
misc_feature 830..832
/note="pot. glycosylation site"
misc_feature 848..850
/note="pot. glycosylation site"
misc_feature 1025..1027
/note="pot. glycosylation site"
misc_feature 1115..1117
/note="pot. glycosylation site"
misc_feature 1289..1291
/note="pot. glycosylation site"
misc_feature 1484..1486
/note="pot. glycosylation site"
misc_feature 1607..1609
/note="pot. glycosylation site"
misc_feature 1709..1711
/note="pot. glycosylation site"
misc_feature 1856..1858
/note="pot. glycosylation site"
misc_feature 1874..1876
/note="pot. glycosylation site"
misc_feature 1907..1909
/note="pot. glycosylation site"
misc_feature 1922..1924
/note="pot. glycosylation site"
misc_feature 2006..2007
/note="PH05 mRNA end"
misc_feature 2008..2357
/note="intergenic region"
misc_feature 2358..2358
/note="transcription initiation site (PH03)"
misc_feature 2361..2361
/note="alternative transcription initiation site (PH03)"
CDS 2392..2442
/note="signal peptide (aa -17 to -1)"
/codon_start=2392
CDS 2443..2443
/note="constitutive acid phosphatase (PH03) (aa 1-430)"
/codon_start=2443
misc_feature 2680..2682
/note="pot. glycosylation site"
misc_feature 2698..2700
/note="pot. glycosylation site"
misc_feature 2875..2877
/note="pot. glycosylation site"
misc_feature 2965..2967
/note="pot. glycosylation site"
misc_feature 3139..3141
/note="pot. glycosylation site"
misc_feature 3334..3336
/note="pot. glycosylation site"
misc_feature 3457..3459
/note="pot. glycosylation site"
misc_feature 3559..3561
/note="pot. glycosylation site"
misc_feature 3706..3708
/note="pot. glycosylation site"
misc_feature 3724..3726
/note="pot. glycosylation site"
misc_feature 3757..3759
/note="pot. glycosylation site"
misc_feature 3772..3774
/note="pot. glycosylation site"
misc_feature 3855..3856
/note="PH03 mRNA end"
BASE COUNT 1220 a 795 c 777 g 1121 t
ORIGIN
1 gatccgaaag ttgtattcaa caagaatgcg caaatatgtc aacgtatttg gaagtcatct
61 tatgtgcgct gctttaatgt tttctcatgt aagcggacgt cgtctataaa cttcaaacga
121 aggtaaaagg ttcatagcgc tttttctttg tctgcacaaa gaaatatata ttaaattagc
181 acgttttcgc atagaacgca actgcacaat gccaaaaaaa gtaaaagtga ttaaaagagt
241 taattgaata ggcaatctct aaatgaatcg atacaacctt ggcactcaca cgtgggacta
301 gcacagacta aatttatgat tctggtccct gttttcgaag agatcgcaca tgccaaatta
361 tcaaattggt caccttactt ggcaaggcat atacccattt gggataaggg taaacatctt
421 tgaattgtcg aaatgaaacg tatataagcg ctgatgtttt gctaagtcga ggttagtatg
481 gcttcatctc tcatgagaat aagaacaaca acaaatagag caagcaaatt cgagattacc
541 aatgtttaaa tctgttgttt attcaatttt agccgcttct ttggccaatg caggtaccat
601 tcccttaggc aaactagccg atgtcgacaa gattggtacc caaaaagata tcttcccatt
661 tttgggtggt gccggaccat actactcttt ccctggcgac tatggtattt ctcgtgattt
721 gcctgaaggt tgtgaaatga agcaactgca aatggttggt agacatggtg aaagataccc
781 tactgtcagt ctggctaaga ctatcaagag tacatggtat aagttgagca attacactcg
841 tcaattcaac ggctcattgt cattcttgaa cgatgattac gagtttttca tccgtgatga
901 cgatgatttg gaaatggaaa ccacttttgc caactcggac gatgttttga acccatacac
961 tggtgaaatg aacgccaaga gacatgctcg tgacttcttg gctcaatacg gttacatggt
1021 cgaaaaccaa accagtttcg ccgtttttac ctctaattct aagagatgtc atgacactgc
1081 tcaatatttc attgatggtt taggtgacca attcaacatc accttgcaga ctgtcagtga
1141 agctgaatcc gctggtgcca acactttgag tgcttgtaac tcatgtcctg cttgggacta
1201 cgatgccaat gatgacattg taaatgaata cgacacaacc tacttggatg acattgccaa
1261 gagattgaac aaggaaaaca agggtttgaa cttgacctca actgacgcta gtactttatt
1321 ctcgtggtgt gcatttgaag tgaacgctaa aggttacagt gatgtctgtg atattttcac
1381 caaggatgaa ttagtccatt actcctacta ccaagacttg cacacttatt accatgaggg
1441 tccaggttac gacattatca agtctgtcgg ttccaacttg ttcaatgcct cagtcaaatt
1501 attaaagcaa agtgagattc aagaccaaaa ggtttggttg agttttaccc acgataccga
1561 tatcctaaac tttttgacca ccgctggtat aattgacgac aaaaacaact taactgccga
1621 atacgttcca ttcatgggca acactttcca cagatcctgg tacgttcctc aaggtgctcg
1681 tgtctacacc gaaaaattcc aatgttctaa cgacacctac gtcagatacg tcattaacga
1741 tgctgttgtt ccaattgaaa cctgttccac tggtccaggg ttctcttgtg aaatcaatga
1801 cttctacgac tatgctgaaa agagagtagc cggtactgac ttcctaaagg tctgtaacgt
1861 cagcagcgtc agtaactcta ctgaattgac cttctactgg gactggaaca ctactcatta
1921 caacgccagt ctattgagac aatagttttg tataactaaa taatattgga aactaaatac
1981 gaatacccaa attttttatc taaattttgc cgaaagatta aaatctgcag agatatccga
2041 aacaggtaaa tggatgtttc aatccctgta gtcagtcagg aacccatatt atattacagt
2101 attagtcgcc gcttaggcac gcctttaatt agcaaaatca aaccttaagt gcatatgccg
2161 tataagggaa actcaaagaa ctggcatcgc aaaaatgaaa aaaaggaaga gtgaaaaaaa
2221 aaaaattcaa aagaaattta ctaaataata ccagtttggg aaatagtaaa cagctttgag
2281 tagtcctatg caacatatat aagtgcttaa atttgctgga tggaagtcaa ttatgccttg
2341 attatcataa aaaaaatact acagtaaaga aagggccatt ccaaattacc tatgtttaag
2401 tctgttgttt attcggttct agccgctgct ttagttaatg caggtacaat tcccctcgga
2461 gagttagccg atgttgccaa aattggcact caggaagaca tattcccatt cctgggtggt
2521 gccgggccat acttctcttt ccctggcgac tatggtattt ctcgtgactt gcctgaaggt
2581 tgtgaaatga aacaattgca aatgcttgcc agacatggtg aaagataccc aacttacagt
2641 aaaggtgcta ccatcatgaa aacatggtat aagttgagca attacacacg tcaattcaac
2701 ggctcattgt cattcttgaa cgatgattac gagtttttca tccgtgatga cgatgatttg
2761 gaaatggaaa ccacttttgc caactcagac aatgttttga atccatacac tggtgagatg
2821 gatgctaaga gacatgctcg tgagttttta gcgcaatatg gctacatgtt cgaaaatcaa
2881 accagtttcc caattttcgc cgctagttct gaaagggttc atgacactgc tcaatatttc
2941 attgatggtt taggtgacca attcaacatc tccttgcaga ctgtcagtga agccatgtcc
3001 gccggcgcaa acactttgag tgctggtaat gcgtgcccag gatggatgaa gactgctaac
3061 gatgacattt tggacaaata cgataccaca tacttggatg acattgccaa gagattaaac
3121 aaagaaaaca agggtttgaa tttgacctca aaggacgcca acactttgtt tgcatggtgt
3181 gcatacgaat tgaacgctag aggctacagt gatgtttgtg atatcttcac cgaagatgaa
3241 ttggtacgtt actcatacgg ccaggacctg gtatcgtttt accaggatgg accaggttat
3301 gatatgatca gatccgtcgg tgccaacttg tttaacgcta ctttgaagtt gttaaagcaa
3361 agtgaaactc aagacttaaa agtctggttg agttttaccc acgataccga tatcctaaac
3421 tatttgacca ccgctggtat aattgacgac aaaaacaact taactgccga atacgttcca
3481 ttcatgggca acaccttcca taagtcctgg tacgttcctc aaggtgctcg tgtctacacc
3541 gaaaaattcc aatgttctaa cgacacctac gtcagatacg tcattaacga tgctgtcgtt
3601 ccaattgaaa cctgttccac cggcccaggg ttctcttgtg aaatcaatga tttctacgac
3661 tatgctgaaa agagagtagc tggtactgac ttcctaaagg tctgtaacgt cagcagtgtc
3721 agtaacgtca ccgaattgac cttctactgg gactggaata ctactcacta caacgatacc
3781 ctattaaaac aataaattgt ataaataaat aatattgcaa atataaataa atacccatcg
3841 attctgatct tcttttatgt actagtaggg taacgagttt gtagattagc ggcagaatga
3901 aaagaaaaaa gtt
//