GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS YSCPHO4M1 1571 bp ds-DNA PLN 17-MAY-1991
DEFINITION Yeast PHO4 gene mutated by in vitro mutagenesis
ACCESSION X52577
KEYWORDS DNA-binding protein; regulatory gene; transcriptional activator.
SOURCE Saccharomyces cerevisiae DNA.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 1571; 868 to 1571)
AUTHORS Bergman,L.W.
JOURNAL Unpublished (1990)
STANDARD full automatic
COMMENT *source: GG100-14D; **map: chromosome=VI R; See also <X52578> for
another mutant and <X03719> for wt gene (although there are
numerous conflicts). The in vitro mutagenesis alters c to t at
position 904 (P -> S).
Data kindly reviewed (03-AUG-1990) by Bergman L.
From EMBL 26 entry SCPHO4M1; dated 02-OCT-1990.
FEATURES Location/Qualifiers
CDS 385..1320
/note="PHO4 mutant protein (AA 1-312)"
/codon_start=385
mutation 904..904
/note="t is c in <X03719>"
BASE COUNT 431 a 400 c 402 g 338 t
ORIGIN
1 aagctttctt gaaacacttt ctcgaacctc aaatcaggta tgttcaaatg tttgttggga
61 gttggcgccg taggtacgtt catttctacg ttcccacagt tttttttctt gctgccttgc
121 cttctttgcg tacggtgtgt atgtgtatgt ctgtgtgcaa gtacttccta cgcagcaact
181 gcgattttct cgttttctat tttttttttt tgcctcgcct aatatgtggt aggcgaaagg
241 ctgacccggc cgctcgcacg gaaatatttg gcaaatgagt cttgaccata gaaaaatgta
301 tataacgacg gaagaagtca tgcttcggaa ggaccagaag agaagagatg agcaaaggag
361 acagaacaag agtagcagaa agtcatgggc cgtacaactt ctgagggaat acacggtttt
421 gtggacgatc tagagcccaa gagcagcatt cttgataaag tcggagactt tatcaccgta
481 aacacgaaac ggcatgatgg gcgcgaggac ttcaacgagc aaaacgacga gctgaacagt
541 caagagaacc acaacagcag tgagaatggg aacgagaatg aaaatgaaca agacagtctc
601 gcgttggacg acctagaccg cgcctttgag ctggtggaag gtatggatat ggactggatg
661 atgccctcgc atgcgcacca ctccccagct acaactgcta caatcaagcc gcggctatta
721 tattcgccgc taatacacac gcaaagtgcg gttcccgtaa ccatttcgcc gaacttggtc
781 gctactgcta cttccgccac atccgctaac aaagtcacta aaaacaagag taatagtagt
841 ccgtatttga acaagcgcaa aggtaaaccc gggccggatt cggccacttc gctgttcgaa
901 ttgtccgaca gcgttatccc aactccgaaa ccgaaaccga aaccaaagca atatccgaaa
961 gttattctgc cgtcgaacag cacaagacgc gtatcaccgg tcacggccaa gaccagcagc
1021 agcgcagaag gcgtggtcgt agcaagtgag tctcctgtaa tcgcgccgca cggatcgacc
1081 catgcgcggt cgctgagtaa gcgacggtca tcgggcgcgc tcgtggacga tgacaagcgc
1141 gaatcacaca agcatgcaga gcaagcacgg cgtaatcgat tagcggtcgc gctgcacgaa
1201 ctggcgtctt taatccccgc ggagtggaaa cagcaaaatg tgtcggccgc gccgtccaaa
1261 gcgaccaccg tggaggcggc ctgccggtac atccgtcacc tacagcagaa cgggagcacg
1321 tgaccgtgca ccaatgggaa gcacgcttcc gggcatatcg gactggggcg cgcctcccct
1381 gcgcggtgct tgttataaga ggcgctttgc tggaaagtgg cccacaccgg gttttcgaga
1441 ttaggaccta ctactcagtc ttaagggcag tattggttgg cgcttatttg cacatattgt
1501 atacacgcac tcacattaac agaagcacac atatacactt acacctacac acacggataa
1561 agaaaaagaa a
//GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS YSCPHO4M2 1571 bp ds-DNA PLN 17-MAY-1991
DEFINITION Yeast PHO4 gene mutated by in vitro mutagenesis
ACCESSION X52578
KEYWORDS DNA-binding protein; regulatory gene; transcriptional activator.
SOURCE Saccharomyces cerevisiae DNA.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 1571; 868 to 1571)
AUTHORS Bergman,L.W.
JOURNAL Unpublished (1990)
STANDARD full automatic
COMMENT *source: strain=GG100-14D; **map: chromosome=VI R; See also
<X52577> for another mutant and <X03719> for wt gene (although
there are numerous conflicts). The in vitro mutagenesis alters c to
t at position 905 (P -> L).
Data kindly reviewed (03-AUG-1990) by Bergman L.
From EMBL 26 entry SCPHO4M2; dated 02-OCT-1990.
FEATURES Location/Qualifiers
CDS 385..1320
/note="PHO4 mutant protein (AA 1-312)"
/codon_start=385
mutation 905..905
/note="t is c in <X03719>"
BASE COUNT 431 a 400 c 402 g 338 t
ORIGIN
1 aagctttctt gaaacacttt ctcgaacctc aaatcaggta tgttcaaatg tttgttggga
61 gttggcgccg taggtacgtt catttctacg ttcccacagt tttttttctt gctgccttgc
121 cttctttgcg tacggtgtgt atgtgtatgt ctgtgtgcaa gtacttccta cgcagcaact
181 gcgattttct cgttttctat tttttttttt tgcctcgcct aatatgtggt aggcgaaagg
241 ctgacccggc cgctcgcacg gaaatatttg gcaaatgagt cttgaccata gaaaaatgta
301 tataacgacg gaagaagtca tgcttcggaa ggaccagaag agaagagatg agcaaaggag
361 acagaacaag agtagcagaa agtcatgggc cgtacaactt ctgagggaat acacggtttt
421 gtggacgatc tagagcccaa gagcagcatt cttgataaag tcggagactt tatcaccgta
481 aacacgaaac ggcatgatgg gcgcgaggac ttcaacgagc aaaacgacga gctgaacagt
541 caagagaacc acaacagcag tgagaatggg aacgagaatg aaaatgaaca agacagtctc
601 gcgttggacg acctagaccg cgcctttgag ctggtggaag gtatggatat ggactggatg
661 atgccctcgc atgcgcacca ctccccagct acaactgcta caatcaagcc gcggctatta
721 tattcgccgc taatacacac gcaaagtgcg gttcccgtaa ccatttcgcc gaacttggtc
781 gctactgcta cttccgccac atccgctaac aaagtcacta aaaacaagag taatagtagt
841 ccgtatttga acaagcgcaa aggtaaaccc gggccggatt cggccacttc gctgttcgaa
901 ttgctcgaca gcgttatccc aactccgaaa ccgaaaccga aaccaaagca atatccgaaa
961 gttattctgc cgtcgaacag cacaagacgc gtatcaccgg tcacggccaa gaccagcagc
1021 agcgcagaag gcgtggtcgt agcaagtgag tctcctgtaa tcgcgccgca cggatcgacc
1081 catgcgcggt cgctgagtaa gcgacggtca tcgggcgcgc tcgtggacga tgacaagcgc
1141 gaatcacaca agcatgcaga gcaagcacgg cgtaatcgat tagcggtcgc gctgcacgaa
1201 ctggcgtctt taatccccgc ggagtggaaa cagcaaaatg tgtcggccgc gccgtccaaa
1261 gcgaccaccg tggaggcggc ctgccggtac atccgtcacc tacagcagaa cgggagcacg
1321 tgaccgtgca ccaatgggaa gcacgcttcc gggcatatcg gactggggcg cgcctcccct
1381 gcgcggtgct tgttataaga ggcgctttgc tggaaagtgg cccacaccgg gttttcgaga
1441 ttaggaccta ctactcagtc ttaagggcag tattggttgg cgcttatttg cacatattgt
1501 atacacgcac tcacattaac agaagcacac atatacactt acacctacac acacggataa
1561 agaaaaagaa a
//