GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS YSCPHO6 2132 bp ds-DNA PLN 17-MAY-1991
DEFINITION Yeast gene PHO5 fragment encoding acid phosphatase (EC 3.1.3.2)
ACCESSION V01320
KEYWORDS phosphatase; signal peptide.
SOURCE Saccharomyces cerevisiae DNA.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 2132)
AUTHORS Arima,K., Oshima,T., Kubota,I., Nakamura,N., Mizunaga,T. and
Toh-e,A.
TITLE The nucleotide sequence of the yeast PHO5 gene: a putative
precursor of repressible acid phosphatase contains a signal peptide
JOURNAL Nucleic Acids Res. 11, 1657-1672 (1983)
STANDARD full automatic
COMMENT SWISS-PROT; P00635; PPA5$YEAST.
Data kindly reviewed (08-JUN-1983) by Arima K.. The N-terminal
amino acids of the mature form were determined by submitting the
purified enzyme to partial Edman degradation. Data kindly reviewed
(31-MAY-1983) by K. Arima.
The N-terminal amino acids of the mature form were determined by
submitting the purified enzyme to partial Edman degradation.
From EMBL 26 entry SCPHO5; dated 06-JUL-1989.
FEATURES Location/Qualifiers
precursor_RNA 1..2132
/note="primary transcript"
CDS 542..1942
/note="acid phosphatase (preform)"
/codon_start=542
CDS 542..592
/note="signal peptide"
/codon_start=542
CDS 593..1942
/note="acid phosphatase (mature)"
/codon_start=593
BASE COUNT 661 a 432 c 422 g 617 t
ORIGIN
1 gatccgaaag ttgcattcaa caagaatgcg caaatatgtc aacgtatttg gaagtcatct
61 tatgtgcgct gctttaatgt tttctcatgt aagcggacgt cgtctataaa cttcaaacga
121 aggtaaaagg ttcatagcgc tttttctttg tctgcacaaa gaaatatata ttaaattagc
181 acgttttcgc atagaacgca actgcacaat gccaaaaaag gtaaaagtga ttaaaagagt
241 taattgaata ggcaatctct aaacgaatcg atacaacctt ggcactcaca cgtgggacta
301 gcacagacta aatttatgat tctggtccct gttttcgaag agatcgcaca tgccaaatta
361 tcaaattggt caccttactt ggcaaggcat atacccattt gggataaggg taaacatctt
421 tgaattgtcg aaatgaaacg tatataagcg ctgatgtttt gctaagtcga ggttagtatg
481 gcttcatctc tcatgagaat aagaacaaca acaaatagag caagcaaatt cgagattacc
541 aatgtttaaa tctgttgttt attcaatttt agccgcttct ttggccaatg caggtaccat
601 tcccttaggc aaactagccg atgtcgacaa gattggtacc caaaaatata tcttcccatt
661 tttgggtggt gccggaccat actactcttt ccctggcgac tatggtattt ctcgtgattt
721 gcctgaaggt tgtgaaatga agcaactgca aatggttggt aggcatggtg aaagataccc
781 tactgtcagt ctggctaaga ctatcaagag tacatggtat aagttgagca attacactcg
841 tcaattcaac ggctcattgt cattcttgaa cgatgattac gagtttttca tccgtgatga
901 cgatgatttg gaaatggaaa ccacttttgg gaactcggac gatgttttga acccatacac
961 tggtgaaatg aacgccaaga gacatgctcg tgacttcttg gctcaatacg gttacatggt
1021 cgaaaaccaa accagtttcg ccgtttttac ctctaattct aagagatgtc atgacactgc
1081 tcaatatttc attgatggtt taggtgacca attcaacatc accttgcaga ctgtcagtga
1141 agctgaatcc gctggtgcca acactttgag tgcttgtaac tcatgtcctg cctgggacta
1201 cgatgccaat gatgacattg taaatgaata cgacacaacc tacttggatg acattgccaa
1261 gagattgaac aaggaaaaca agggtttgaa cttgacctca actgacgcta gtactttatt
1321 ctcgtggtgt gcatttgaag tgaacgccaa aggttacagt gatgtctgtg atattttcac
1381 caaggatgaa ttagtccatt actcctacta ccaagacttg cagacctatt accatgaagg
1441 tccaggttac gatattatca agtctgtcgg ttccaacttg ttcaatgcct cagtcaaatt
1501 attaaagcaa agtgagattc aagaccaaaa ggtttggttg agttttaccc acgataccga
1561 tatcctaaac tttttgacca ccgctggtat aattgacgac aaaaacaact taactgccga
1621 atacgttcca ttcatgggca acactttcca cagatcctgg tacgttcctc aaggtgctcg
1681 tgtctacacc gaaaaattcc aatgttctaa cgacacctac gtcagatacg tcattaacga
1741 tgctgtcgtt ccaattgaaa cctgttccac tggtccaggg ttctcttgtg aaatcaatga
1801 cttctacgac tatgctgaaa agagagtagc cggtactgac ttcctaaagg tctgtaacgt
1861 cagcagtgtc agtaacgtca ccgaattgac cttctactgg gactggaata ctactcacta
1921 caatgatacc ctattaaaac aataaattgt ataaataaat aatattgcaa atataaatac
1981 ccatcgattc tgatcttctt ttatgtacta gtagggtaac gagtttgtag attagcggca
2041 gaatgaaaag aaaaaagtta actaaaaagt aagaacagca aagttgattg tagcctatat
2101 tgctgaaata gatttcggag ctcccgtata at
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