GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS YSCRPO22 6224 bp ds-DNA PLN 17-MAY-1991
DEFINITION Yeast gene RPO21 for RNA polymerase II largest subunit
ACCESSION X03128
KEYWORDS RNA polymerase; RNA polymerase II; polymerase.
SOURCE Saccharomyces cerevisiae DNA.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 6224)
AUTHORS Allison,L.A., Moyle,M., Shales,M. and Ingles,C.J.
TITLE Extensive homology among the largest subunits of eukaryotic and
prokaryotic RNA polymerase
JOURNAL Cell 42, 599-610 (1985)
STANDARD full automatic
REFERENCE 2 (bases 1 to 6224)
AUTHORS Shales,M.
JOURNAL Unpublished (1986)
STANDARD full automatic
COMMENT SWISS-PROT; P04050; RPO2$YEAST.
Data kindly reviewed (29-OCT-1986) by Shales M.
From EMBL 26 entry SCRPO21; dated 06-JUL-1989.
FEATURES Location/Qualifiers
promoter 146..150
/note="pot. TATA-box"
CDS 313..5490
/note="put. RNA polymerase II largest subunit (aa 1-1726)"
/codon_start=313
misc_feature 433..433
/note="pot. altern translation start site"
misc_feature 4900..5445
/note="seq. containing 26-times repeated heptapeptide
(consensus: Pro-Thr-Ser-Pro-Ser-Tyr-Ser)"
misc_signal 5538..5570
/note="seq. put. implicated in transcription termination"
misc_feature 5578..5583
/note="pot. polyA signal"
BASE COUNT 1903 a 1230 c 1214 g 1877 t
ORIGIN
1 gaattccctg atcaactttc aaggaaaaac taaaactact gtattataag aggttttttc
61 acttccagat taattttgaa atacgatatc ctcaagttta tctaccagaa tatttgacta
121 agaaatcaaa ctctgttaat aataatataa ttataaaaac ctcaactaga aactccaaaa
181 aaaaaaattt accatttttt actttctatc cttgttaacc aaatttcaaa aaaattttac
241 cttttctttt tccagaagag ggaccaatca taaagatagt aataacactt taccccaaaa
301 tataaatcag acatggtagg acaacagtat tctagtgctc cactccgtac agtaaaagag
361 gtccaattcg gtcttttctc acctgaagaa gttagagcaa tcagtgtggc caaaattagg
421 tttccagaga caatggatga aacccagacg agagcgaaaa ttggtggtct aaacgaccct
481 aggttaggct ctattgatcg taatctgaag tgtcaaactt gtcaagaggg tatgaacgaa
541 tgtcctggtc attttggtca catagattta gcaaaacctg tatttcatgt tggttttatt
601 gccaaaatta agaaagtatg tgagtgtgtc tgtatgcact gtggtaagct attactggat
661 gaacataatg aattaatgag acaagctcta gcaatcaaag acagtaaaaa aaggtttgct
721 gcaatttgga ctttatgtaa aacaaaaatg gtctgcgaaa cagatgtccc ttctgaagat
781 gaccctactc agctcgtatc aaggggaggt tgtggtaata cacagcctac aattcgtaag
841 gatgggttga aattagttgg tagttggaaa aaagatagag ccacggggga tgcggatgaa
901 ccagaactaa gagttttaag tacggaggaa atcttgaata tttttaagca tatctcagta
961 aaagacttca ctagtttggg tttcaacgaa gttttttctc gtccagaatg gatgatttta
1021 acatgccttc ctgtcccacc accaccggtg cgtccatcca tttccttcaa tgaatctcaa
1081 agaggtgagg atgatttaac ctttaaactt gctgatattt taaaagctaa tattagtttg
1141 gaaacactag agcataacgg tgctccacat catgctattg aagaagcaga gagtttatta
1201 caatttcatg ttgccactta tatggataat gatattgctg gtcaaccaca agctcttcaa
1261 aagtccggcc gtcccgttaa atctattcgt gctcgtttga agggtaaaga gggtcgtatc
1321 agaggtaatt taatgggtaa gcgtgtggat ttttcggcaa gaactgttat ttctggtgat
1381 cctaatttgg aattagacca agtcggtgtt ccaaaatcta ttgccaagac tttaacatac
1441 ccagaagtgg tcacaccata taacatagat cgtctgacgc aacttgttag gaatggacca
1501 aatgaacacc ccggtgccaa atacgtcatt cgtgatagcg gagaccgtat agatttaaga
1561 tacagtaaaa gggcaggtga tattcaatta cagtatgggt ggaaagttga acgtcatatt
1621 atggacaatg atccagtttt attcaaccgt caaccttcgt tgcacaaaat gtccatgatg
1681 gcccacagag taaaagttat tccatattct acatttagat tgaatttgtc cgttacatct
1741 ccatacaatg ccgatttcga cggtgacgaa atgaatcttc acgttcctca gtctgaggaa
1801 acaagggcgg aactttctca attatgtgct gttcctctac aaattgtttc accacaatct
1861 aacaaacctt gtatgggtat tgttcaagat actttgtgtg gtattcgtaa actgacatta
1921 agagatacat ttatagaact tgatcaagtt ttgaatatgc tttattgggt tccagattgg
1981 gatggtgtta ttccgacacc tgcaattatc aagcccaaac ctttgtggtc cggtaaacaa
2041 atcttgtctg tggctatccc aaacggtatt catttacaac gttttgatga gggcactact
2101 ctgctttctc caaaggataa tggtatgctt attattgacg gtcaaatcat ttttggtgta
2161 gtagagaaaa aaaccgttgg ttcctccaat ggtggtttaa ttcatgttgt tacaagagaa
2221 aagggacctc aagtttgtgc taagttgttt ggtaacatac agaaagttgt taacttttgg
2281 ttactacata atgggttttc aacaggtatt ggtgatacca ttgcggacgg cccaacaatg
2341 agggaaatta cagagacaat tgcagaggct aaaaagaaag ttttggatgt tacgaaagaa
2401 gcccaggcaa acttattgac tgctaaacat ggtatgactc tccgtgagtc ttttgaggat
2461 aacgttgttc ggttcctaaa tgaagcaaga gataaggcag gtcgtttagc tgaagtcaat
2521 ttgaaagatt tgaacaatgt gaaacaaatg gttatggcag gttccaaggg ttcatttatt
2581 aatatcgcgc aaatgtcagc ttgtgtagga cagcaatctg ttgaaggtaa acgtattgct
2641 tttgggttcg ttgatcgtac cttacctcat ttctctaaag atgattactc cccagagtct
2701 aaaggttttg ttgagaactc atatttgaga ggtttgaccc cacaagaatt ttttttccat
2761 gcaatgggtg gtcgtgaagg tcttatcgat accgccgtca aaacagccga aacaggttat
2821 attcaacgtc gtttagtgaa agctctagaa gatatcatgg ttcattacga taacaccaca
2881 agaaactcat tgggtaacgt tattcagttt atttatggtg aagatggtat ggatgctgcg
2941 catattgaaa agcaatcgct agatactatt ggtggctccg atgcagcttt tgaaaagaga
3001 tacagagttg atttattgaa tacagaccat acccttgatc cctcactatt ggaatccgga
3061 tctgagatac ttggcgattt gaaacttcaa gttctcctgg atgaagaata caaacaatta
3121 gtgaaagatc gtaaattttt gagggaagtt tttgttgatg gtgaagcaaa ctggccatta
3181 ccagtcaaca taagacgtat tattcaaaat gctcaacaaa ctttccacat agatcatacg
3241 aaaccatctg atttaacaat caaagacatc gttcttggtg taaaggattt gcaagaaaac
3301 ttattagtgt tgcgtggtaa gaatgaaatt atacaaaatg cccagcgaga tgcagttaca
3361 ttgttctgct gtttattacg ttcccgtttg gccacacgta gagttctgca agagtacaga
3421 ctaacaaaac aggcattcga ttgggtatta agtaatatcg aggcacaatt cctccgttct
3481 gttgttcacc ctggtgaaat ggttggtgtt ctagcagccc aatccattgg tgaaccagcc
3541 acacaaatga cccttaacac cttccatttt gctggtgttg cttccaaaaa agttacttct
3601 ggtgtccccc gtttaaagga aattttgaat gtggccaaaa acatgaaaac gccttccttg
3661 actgtatact tagagcctgg tcatgctgcc gatcaagaac aagcgaagtt gatcagatct
3721 gctatcgagc ataccacttt aaagagtgtc actattgctt cagaaattta ctatgatcct
3781 gatccacgtt ccacagttat tccagaagat gaagaaatta tccaacttca tttctcatta
3841 ttggatgaag aagctgaaca atcttttgac caacaatcac cttggttatt acgtctggaa
3901 ctggatcgtg cagcaatgaa tgataaagac ttaacaatgg gtcaggttgg tgaaagaatc
3961 aagcaaacat tcaaaaatga tttgtttgtt atctggtctg aagacaacga tgagaagttg
4021 atcatccgtt gtcgtgttgt tcgtccaaag tcactagatg ctgagactga agcagaagaa
4081 gatcatatgt tgaagaaaat tgagaacaca atgttagaga atattacatt acgtggtgta
4141 gagaacatcg agcgtgttgt catgatgaaa tatgaccgta aagtaccaag tccaactggt
4201 gaatacgtta aggaacctga atgggtgttg gaaacagatg gtgttaactt atctgaagtt
4261 atgactgttc ctggtatcga cccaaccaga atctatacca actccttcat tgatataatg
4321 gaagttctag gtattgaagc tggtcgtgca gccttgtata aagaagttta caatgttatt
4381 gcttctgatg gttcgtatgt taactaccgt catatggctt tgttagtcga tgttatgaca
4441 acccaaggtg gcttaacttc tgttactcgt catggtttca acagatcaaa tacaggtgcc
4501 ttaatgagat gttcatttga agaaactgtc gaaattttgt ttgaagctgg tgcttcagcc
4561 gaattagatg attgtcgtgg tgtttcggaa aatgtcattc ttggtcaaat ggctccaatt
4621 ggtaccggtg catttgatgt gatgatcgat gaggagtcac tggtaaaata catgccagaa
4681 caaaaaataa ctgagattga agacggacaa gatggtggcg tcacaccata cagtaacgaa
4741 agtggtttgg tcaatgcaga tcttgacgtt aaagatgagc taatgttttc acctctggtt
4801 gattcgggtt caaatgacgc tatggctgga ggatttacag cgtacggtgg tgttgattat
4861 ggtgaagcca cgtctccatt tgctgcttat ggtgaagcac ctacatctcc cggatttgga
4921 gtctcctcac caggcttttc tccaacttcc ccaacatact ctcctacctc tccagcgtac
4981 tcaccaacat caccatcgta ctcgccaaca tcaccatcgt attcaccaac gtcaccatca
5041 tattcgccaa cgtcaccatc atattcgcca acgtcgccat cgtattctcc aacgtcacca
5101 tcgtattcgc caatgtcgcc ttcctactct cccacgtcgc caagctacag ccctacgtcg
5161 ccaagctaca gccctacgtc tccttcttat tctcctacat ctccatcata ctctcctacg
5221 tcaccaagtt acagcccaac gtcaccaagt tacagcccaa cgtctccagc ctattcccca
5281 acatcaccaa gttatagtcc tacatcgcct tcatactctc caacgtcacc atcctattcc
5341 ccaacatcac cttcttactc tcccacctct ccaaactata gccctacttc accttcttac
5401 tccccaacat ctccaggcta cagcccagga tctcctgcat attctccaaa gcaagacgaa
5461 caaaagcata atgaaaatga aaattccaga tgatatagta tatcatcctt acgtatttga
5521 cgttattaca ttatatatag tttctcaaat aatatttcta gtttattttt gtatcataat
5581 aaaaacgtat accaaatata ccattatttt tcataacatt atggtaggga tagggaatca
5641 agtaactaat ttatatccgc agagcattgg gaaaaccaac ggcgctagta aatgcattta
5701 aattacgtcc gtccaacttc taagcttcaa tggtagactc ttaactctga cctttttagc
5761 aattaagctc ttgaagatat caaaagtgtt accgtccggc tgtaaattat aaacgtttcc
5821 tgtaaattga gtggaatacc gcttaccatt cttttgcaat cagtaaaccg tagtcttccg
5881 tgataccagt aatcatggct tgcgtatttc cgtgatctgg taatgttact atttggttac
5941 tatgtaacac aactcataat aacttggcaa tatttccgca gctccgtagt taataaactg
6001 ttttaatatg acctcaaggt tattcatata gagtgcctgc agtttttctg cctttattgc
6061 tggcaataaa tcaaggtgta attgttggcg ttcttcattc aggatatcaa tccaagtttg
6121 taatgaagtt gtaggaccat cactagtcaa atttatacca cagccaagta gcaaacaata
6181 tttattgttt atgaagtggg tattaactaa taaaccagag atct
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