GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS YSCSPO15G 2761 bp ds-DNA PLN 17-MAY-1991 DEFINITION S. cerevisiae SPO15 gene coding for a putative GTP binding protein. ACCESSION X54316 KEYWORDS GTP-binding protein; microtubule binding protein. SOURCE Saccharomyces cerevisiae DNA. ORGANISM Saccharomyces cerevisiae Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes; Endomycetales; Saccharomycetaceae. REFERENCE 1 (bases 1 to 2761) AUTHORS Bloom,K.S. JOURNAL Unpublished (1990) STANDARD full automatic REFERENCE 2 (bases 305 to 2761) AUTHORS Rothman,J.H., Raymond,C.K., Gilbert,T., O'Hara,P.J. and Stevens,T.H. TITLE A putative GTP binding protein homologous to interferon-inducible Mx proteins performs an essential function in yeast protein sorting JOURNAL Cell 61, 1063-1074 (1990) STANDARD full staff_review COMMENT *source: strain=J17; clone=pYe(MET14)2; *source: library= plasmid-Nasmyth and Reed; **map: chromosome=XI; The SPO15 protein binds microtubules and is homologous to VPS1. See also M33315 (reference [3]). From EMBL 26 entry SCSPO15G; dated 10-JAN-1991. FEATURES Location/Qualifiers conflict replace(:415..415,:719..719) /citation=[2] conflict replace(:648..648,:952..952) /citation=[2] conflict replace(:650..650,:954..954) /citation=[2] conflict replace(:738..738,:1042..1042) /citation=[2] mat_peptide 622..2733 /product="GTP-binding protein" /gene="SPO15" CDS 622..2736 /product="GTP-binding protein" /gene="SPO15" /codon_start=622 BASE COUNT 901 a 511 c 542 g 807 t ORIGIN 1 atccttcctt tttcagttct tggattaaac tatcatttaa tttattttct gctgctgtgg 61 cgcccacggt ggaaacaggt ccagtaatac caaaaacctt ttgagagctc attatttgtc 121 taaaaaaact ttatgcgtta aatattttgc taattctagg cctcttttga ttcaaaaaag 181 accgtttaaa gacttgctca cagtgaaatt atttagctag tttagtgtct ttaaactttt 241 gctaaaacgt ttcacttgaa aaatcgttct aatatcgtcc agaggatagt atgatagctt 301 cagagaattc gatagatact tgaatcctct aatagtcgaa aaatgctcga gggtaaacca 361 cttgtgcgct tggactggcc tagtttccaa aaccaatgtt ctaatggatt gatttcttcc 421 ccaaacatta ttaagtggcc gggtcaccca aagacttggg cgccgttgat tcgcgtcgct 481 ttgccatcaa gagaacaaca tatcttccaa gacaaaccga gataattcat ctatttactc 541 ctaaaaaaga attagagagg ccttttatag caccaaaata aggaccgtac gaaaactgca 601 cattttatat tatcagatat catggatgag catttaattt ctactattaa caagcttcag 661 gacgctttgg cgcccttagg aggaggatct caatctccta ttgatttacc acagatcact 721 gttgtcggtt cccagtcgtc aggaaagtcg tccgttttgg agaacattgt tggtagggat 781 ttcttgccaa gaggtactgg tattgtcacc aggagacctt tagtgttaca attgattaat 841 aggagaccaa aaaagtcaga acatgctaaa gtaaaccaaa ctgctaatga attgattgac 901 ttgaacatca acgatgatga caagaaaaag gatgaatcag gaaagcacca gaacgaggga 961 caatctgaag acaataaaga ggaatggggt gaatttttgc atttacctgg taagaagttt 1021 tataattttg acgaaattag acaggaaatc gtcaaagaaa ctgacaaagt gacaggtgcc 1081 aattcaggta tttcttctgt gcccattaac ttgagaattt attctccgca tgttcttact 1141 ttgacgttag tggatttgcc tgggttgacg aaggttcccg taggtgacca acctcctgat 1201 attgaaagac aaattaagga catgttgtta aagtatattt cgaaaccaaa cgctatcata 1261 ttatctgtta atgccgctaa caccgattta gccaacagcg atggtttgaa gctggctaga 1321 gaggtcgatc cagaaggaac gagaactatt ggtgtcttga caaaagtcga tttgatggat 1381 caaggtacag atgtcataga tattttggct ggaagagtca ttcctttgag atatggttat 1441 atcccagtta tcaatagagg tcaaaaggat attgaacaca aaaaaacaat cagagaagcc 1501 cttgaaaacg aaagaaaatt ttttgagaac catccctctt acagttctaa agctcattac 1561 tgtggtacac catatttggc taaaaagtta aactcaatct tattacacca cattaggcaa 1621 actctgccag aaatcaaagc gaaaatcgaa gccacattga aaaaatatca aaacgaactt 1681 ataaacttgg gcccagaaac tatggattca gctagttcgg ttgttttgag catgattact 1741 gatttttcca atgaatatgc cggtatcttg gacggtgagg cgaaggagct ttccagtcag 1801 gaactttctg gtggtgctag aatttcttac gtattccatg aaactttcaa aaatggtgta 1861 gactctttgg atccattcga ccagatcaaa gattctgata tcagaaccat tatgtacaat 1921 agttcaggtt ctgccccatc tttgtttgtc ggtaccgaag cttttgaagt tttagttaaa 1981 cagcaaatta gaagatttga agaaccatct ctacgtttag ttactctggt gtttgatgaa 2041 cttgttcgta tgctaaaaca gattatttca caaccaaagt actcaaggta tcctgctcta 2101 agagaagcga tttctaatca gttcattcag ttcttaaagg atgctactat tcctacgaat 2161 gagtttgttg tcgatatcat caaagctgaa caaacttaca tcaatacagc ccatcccgac 2221 cttttgaagg gttctcaagc aatggttatg gtggaagaaa aattacatcc tcgccaagtc 2281 gctgttgacc caaagacggg taaaccatta ccaacccaac catcgtctag taaggcgcca 2341 gttatggaag agaaatcagg attttttggt gggttcttct ccactaaaaa caagaagaaa 2401 ttggcagctt tggaatcccc acctcctgtt ttaaaagcta ctggccaaat gacagagagg 2461 gaaacaatgg aaacagaagt aatcaagttg ttgattagta gttatttctc tattgtcaaa 2521 agaaccattg ccgatattat accaaaggct ttgatgctta aattgattgt gaaaagtaaa 2581 actgatattc agaaagtttt actcgaaaaa ctttacggaa agcaagatat tgaagaatta 2641 acgaaagaaa acgacataac cattcaaaga agaaaagaat gtaagaagat ggtcgagata 2701 ttgagaaacg ctagtcaaat cgtctcctct gtttaggttt tcctcatcta taccggtcga 2761 c //