GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS YSCTRP5B 798 bp ds-DNA PLN 17-MAY-1991
DEFINITION Part of the yeast gene for tryptophan synthetase. A copy of the
insertion element IS2 is inserted after cloning in E.coli by
duplicating a 5 base pair sequence. This is the wildtype TRP5 gene
before insertion of IS2.
ACCESSION V01343 J02561 J02562
KEYWORDS insertion sequence; synthetase.
SOURCE Saccharomyces cerevisiae DNA.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 798)
AUTHORS Brosius,J. and Walz,A.
TITLE DNA sequences flanking an E.coli insertion element IS2 in a cloned
yeast trp5 gene
JOURNAL Gene 17, 223-228 (1982)
STANDARD full automatic
COMMENT SWISS-PROT; P00931; TRP$YEAST.
From EMBL 26 entry SCTRP5B; dated 22-JAN-1991.
FEATURES Location/Qualifiers
misc_feature 217..221
/note="5 bp duplicated (IS2 insertion)"
CDS 281..>798
/product="put. tryptophan synthetase"
/gene="trp5"
/codon_start=281
BASE COUNT 233 a 168 c 150 g 247 t
ORIGIN
1 tctagacctg aacaatctgg aattaggtgg tctgaacatg gatttcttgt gactggtgca
61 tctatgtatc caagatagta attatctgta taatcttcta ttctttatac cttattaccc
121 gaaaggcttt tctttttatt ttttattttt tttcacttct tgaccgtgga agaatgacta
181 attttactat ttgtcatata agaatcatcg agattcttac actttttatg gactgtaaac
241 atcgattgct ccaaaaaggg acatagcaca ccgacagacc atgtcagaac aactcagaca
301 aacatttgct aacgctaaaa aagaaaacag gaacgccttg gtcacattta tgaccgcagg
361 ttacccaaca gtcaaagaca ctgtccctat tctcaagggt ttccaggatg gtggtgtaga
421 tatcatcgaa ttgggtatgc ccttctctga tccaattgca gatggtccta caattcaatt
481 atctaatact gtggctttgc aaaacggtgt taccttgcct caaactctag aaatggtctc
541 ccaagctaga aatgaaggtg ttaccgtacc cataatccta atgggttact ataaccctat
601 tctaaactac ggtgaagaaa gatttattca ggacgctgcc aaggctggtg ctaatggttt
661 tatcatcgtc gatttgccac cagaggaggc gttgaaggtc agaaactaca tcaatgataa
721 tggtttgagc ctgatcccac tagtggctcc ttctaccacc gacgaaagat tggaattact
781 atcgcatatt gccgattc
//