GenBank-Updates@genbank.bio.net (05/17/91)
LOCUS YSCUBR1G 6873 bp ds-DNA PLN 17-MAY-1991
DEFINITION Yeast UBR1 gene for ubiquitin-protein ligase (E3)
ACCESSION X53747
KEYWORDS ligase; ubiquitin-protein ligase.
SOURCE Saccharomyces cerevisiae DNA.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 6873)
AUTHORS Bartel,B.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 6873)
AUTHORS Bartel,B., Wuenning,I. and Varshavsky,A.
TITLE The recognition component of the N-end rule pathway
JOURNAL EMBO J. 9, 3179-3189 (1990)
STANDARD full automatic
COMMENT *source: strain=GRF88 (S288C); library=YCp50; **map: chromosome=XV;
Data kindly reviewed (28-NOV-1990) by Bartel B.
From EMBL 26 entry SCUBR1G; dated 06-DEC-1990.
FEATURES Location/Qualifiers
CDS 713..6562
/note="ubiquitin-protein ligase (E3)"
/codon_start=713
BASE COUNT 2293 a 1215 c 1293 g 2072 t
ORIGIN
1 aaaagggtta tccagttgcc acaccacaaa agtcgaaaaa ggctaagaaa ccaaagaata
61 aggtactaag tacccaggcg ctactaagac caacgagatt gccacgaaac tagaggaaac
121 caaattgtaa gcatagctta atccgttttc acgattcata atataataaa taagaaaaga
181 tatatcatat aaacgttata aaattaataa ccgggtaagt gtagaaaagt gatgcgacgg
241 tttattttct cttcctcttg cgattgaatt taacttgcag atagtgacca taaggcaact
301 acccagtggc aaacagtttt gataacgccc agtacatcaa cgagcgagta taaagacttt
361 ggtacatttt aaaaaggaaa catatattgt tttcattgct agaccctttt agtctcacct
421 caataaaact gctttattcc tcattgggct ttttattctt taattttgca tacttatagc
481 gtgaaactgg gcatttaaca aaagcaaact attttaatag tagcatcctg ctttctttgc
541 ccctccttct tattgcgata cattattaag tttttttacc acctttcttc ctttttcttc
601 gcatcttcgg acaaaggcag ttgaagttta ctgtatccta ttagttgact attttctctc
661 actgaagtcc ctaatcttta caggtcacac aaattacata gaacattcca atatgtccgt
721 tgctgatgat gatttaggat ctttacaagg tcacattagg agaacactga ggtctattca
781 taacctcccc tattttaggt atacgagagg tcctactgaa agggctgaca tgagcagagc
841 ccttaaagag ttcatttaca gatatctata ctttgtcatt tctaacagcg gagagaactt
901 acctacttta ttcaatgctc atccaaaaca aaaattatct aacccagagc ttactgtttt
961 tcctgacagt ttagaagatg ctgtggatat tgataagata acatctcaac aaactattcc
1021 gttttataag atagatgaat ccagaatagg agacgtccat aaacataccg gaagaaattg
1081 tgggaggaaa ttcaaaatag gggaaccctt gtataggtgt catgagtgtg gttgcgatga
1141 tacttgtgtg ctttgtattc attgttttaa tccaaaagat catgtgaatc atcatgtttg
1201 taccgatata tgtactgaat tcactagtgg aatttgtgac tgtggagatg aagaagcttg
1261 gaattctcca cttcattgca aagctgaaga acaagaaaac gatatatcgg aagatcccgc
1321 cacaaatgcc gacataaagg aagaagatgt atggaatgat tcagtgaata tagcattagt
1381 ggagttggtt cttgcagaag tgtttgacta ttttatcgat gtttttaacc agaatatcga
1441 acctctaccc acaatacaga aagatatcac tatcaaatta agagagatga cacaacaggg
1501 aaaaatgtat gaaagagcgc agtttttaaa cgatttaaaa tatgaaaacg attatatgtt
1561 tgacggaaca acaacagcaa agacgagccc ctctaatagc cctgaggcat ctccatcgct
1621 tgcgaaaatc gatcccgaaa attacacggt aataatatat aacgatgaat atcataacta
1681 ttcgcaggct acgactgcct tgagacaggg ggttccagat aacgtacaca ttgatctatt
1741 aacatccagg attgacggag aaggtcgagc aatgttgaaa tgttcacaag atttatcatc
1801 tgtcttaggt ggtttttttg ctgttcaaac gaatggatta agtgccactt taacatcgtg
1861 gtcagaatat cttcatcaag aaacatgcaa gtatatcatc ctttggatta cacactgttt
1921 aaacatacca aactcctcct tccaaaccac ttttaggaac atgatgggta aaaccctgtg
1981 ttcagaatac ttaaatgcaa cagaatgtcg tgatatgaca ccggtggtgg agaaatattt
2041 cagcaacaag ttcgataaga atgatcccta cagatacatc gatttatcta tccttgcaga
2101 tggcaatcaa attcctttgg gtcatcacaa aattctaccc gaatcaagca cccattcact
2161 ttcaccattg ataaatgatg tagaaactcc aacatcaaga acatattcta atacaagatt
2221 acaacatatt ctttattttg ataacagata ttggaaaaga ctgagaaaag atattcagaa
2281 tgtgatcatt ccaacgttgg cctcttcaaa tttatacaag cccattttct gtcaacaggt
2341 agtcgaaata ttcaaccaca tcacaagatc tgtggcatat atggatagag aacctcagct
2401 aacagctata agagagtgtg tcgtacagtt atttacatgc cctacgaatg ccaaaaatat
2461 tttcgaaaac caaagttttt tggatattgt gtggtcaatt atagacattt ttaaggagtt
2521 ttgtaaggtg gaaggtggtg tattgatctg gcagagagtt caaaaaagca atttaacaaa
2581 gagttacagt atatcattca agcaagggtt gtatacagtt gaaactttat tgagtaaggt
2641 acatgatcct aatattccat tgagacctaa ggaaataata tctctattaa ctctttgcaa
2701 gctcttcaat ggagcgtgga agataaagcg taaagaaggt gaacatgttc ttcacgaaga
2761 tcaaaatttc atctcctatt tagaatatac cacctccatt tacagtatta ttcaaacagc
2821 ggaaaaagtt agcgaaaaat caaaagattc cattgattca aagctttttt taaatgcaat
2881 aagaattatc agttcatttc taggcaatag atcactaaca tacaagttaa tatatgattc
2941 tcatgaagtc attaagttta gcgtaagcca cgaaagagtt gcatttatga atcctttaca
3001 aacaatgctt tcattcctta tcgaaaaagt gtctttaaag gatgcatacg aagcgttaga
3061 agactgttca gattttctga aaatatcgga tttctctttg agatcagttg tattgtgctc
3121 tcaaattgac gtcgggtttt gggtaagaaa tggtatgtct gtgttacatc aagcatcgta
3181 ttataaaaac aatcccgaat taggttccta ttccagagat atacatttga atcagttagc
3241 tatcctttgg gaacgtgatg acattccgag aataatatac aatattcttg atagatggga
3301 attactagac tggtttacag gtgaagttga ctatcaacac actgtctatg aggataaaat
3361 ttcattcatc attcaacaat tcattgcctt tatttatcaa atattgaccg aaagacaata
3421 ttttaaaact ttctcttctc taaaggatag aagaatggat caaatcaaaa attcaatcat
3481 ttataacctt tacatgaaac ccctttcgta ttcaaagcta ctaagatcag ttcctgacta
3541 cctaactgaa gatactaccg agtttgacga agcacttgaa gaagtctctg ttttcgttga
3601 gcctaagggc ttggccgaca atggtgtttt taaattaaaa gcaagcttgt atgcaaaggt
3661 tgatccgtta aagttgctga atttggaaaa cgaattcgaa agtagtgcaa ctattattaa
3721 atcacatttg gctaaggata aggatgaaat agcaaaagtt gtactaatac cccaagtctc
3781 aataaaacag ttagataaag acgctctcaa tctgggtgct tttacaagga atacggtatt
3841 tgccaaagtg gtctataaac tactccaggt atgtctggat atggaggata gcactttcct
3901 gaatgagcta ctacatttag ttcatggtat attcagggat gatgaattga taaatggtaa
3961 ggactctata ccagaagctt atttgtcaaa gcctatatgt aatttactct tgagcatagc
4021 caatgctaaa tcagacgtat tttccgagag tattgtccgt aaagccgact atttgttaga
4081 gaagatgata atgaaaaaac caaatgaact ttttgagtca ttaattgcca gttttggcaa
4141 ccaatacgtt aatgattata aggataagaa actacgccaa ggagttaact tacaagagac
4201 agagaaggaa cgaaaaagaa gattggccaa gaaacatcaa gcaaggcttc tagccaagtt
4261 taacaatcaa caaacgaaat tcatgaaaga acatgagtct gaatttgatg agcaggataa
4321 cgatgttgac atggtcggtg aaaaagtata tgaatcggag gattttacct gcgcactatg
4381 tcaagattcc agttcgaccg atttttttgt gatacctgcg taccatgatc attccccgat
4441 atttagacct ggtaatattt ttaacccaaa tgaatttatg cccatgtggg atggtttcta
4501 caacgatgat gaaaaacaag cttatattga cgatgatgtt ttagaggctt tgaaggaaaa
4561 cggtagttgt ggatctagga aagtatttgt atcctgtaat catcacattc atcataactg
4621 cttcaagagg tacgttcaaa aaaagagatt ttcatcaaac gcgtttatat gtcctttatg
4681 ccaaacattt tcgaattgta ctttacctct ttgtcaaacc tcaaaggcaa atactggttt
4741 gtcgctagat atgtttttgg aatcagaact ttctttggat acattgtcaa ggctcttcaa
4801 accttttact gaggaaaatt atcgtacaat aaactctata ttttcgttga tgatatctca
4861 gtgtcaaggt tttgataaag cggtaagaaa gcgggccaac ttttcgcata aggacgtttc
4921 tttaatatta agtgtgcact gggcaaatac catttcaatg ttagaaattg cttctaggtt
4981 agaaaagcca tacagtattt catttttcag aagcagggaa cagaaataca aaactttaaa
5041 aaatatatta gtctgcatta tgctgttcac ctttgtcata ggcaagccaa gtatggagtt
5101 tgaaccgtac ccgcagcagc ccgatactgt ttggaaccaa aatcaactct tccaatatat
5161 tgttcgaagc gctttatttt ctccagtttc acttcgtcag actgttacag aggcgttaac
5221 aacgttctct agacagtttc taagagattt tcttcagggt ctttctgatg cagaacaggt
5281 taccaaattg tatgctaaag cgtccaaaat tggggatgtt cttaaagtaa gtgagcagat
5341 gctcttcgcc ctaaggacca tatctgatgt acgaatggag ggattggact ccgaaagtat
5401 tatttatgat ctagcttata ccttcctgtt gaagagtctt cttcctacga taagaaggtg
5461 cttagtcttt atcaaagtgc tacacgaatt agtgaaagat tctgaaaatg aaacgctggt
5521 catcaacggt catgaggttg aagaagaact ggaattcgaa gataccgccg agtttgtcaa
5581 taaggcattg aaaatgatta ctgaaaaaga atccttagtc gatcttttga caacgcagga
5641 gtccattgta tcgcatccat atttggagaa catcccatat gaatattgtg ggattataaa
5701 actgatagat ttatcaaaat atttgaatac ttacgttacc caatctaagg aaatcaagct
5761 acgtgaagag cgttctcagc atatgaagaa tgcggataat cgattagatt tcaaaatatg
5821 tttgacatgt ggtgtcaaag tgcatttaag agcggaccgc catgaaatga ccaagcattt
5881 gaataaaaat tgttttaaac catttggcgc cttcctgatg cccaattcaa gtgaagtttg
5941 tctccatttg acacagcctc cttctaatat ttttatatct gcaccatatc taaattcgca
6001 tggtgaagtt ggtagaaatg ccatgagaag aggtgattta accaccttga atttgaaaag
6061 atatgagcac ttaaacagat tatggattaa caatgaaatt ccaggttata ttagtagagt
6121 aatgggtgac gaatttcgag tgactatact ttccaatggt ttcttgtttg catttaatag
6181 agaaccaagg ccaagaagaa tacctccaac agatgaagat gatgaagata tggaagaggg
6241 agaagacggg ttttttacgg agggaaatga cgaaatggat gttgatgatg aaactggaca
6301 ggccgccaac ttatttgggg ttggcgcaga aggtattgct ggtggcggag tacgtgattt
6361 tttccaattc ttcgaaaact ttaggaacac tctacaaccg cagggaaatg gcgatgatga
6421 tgcacctcag aatcccccac caatattgca gttcttagga cctcaatttg atggagctac
6481 tatcataaga aatacaaatc caagaaacct cgatgaggat gactctgatg ataatgatga
6541 ctctgatgag cgagagattt ggtagattca agccctctac tatgttttat agttgacata
6601 tttgtatata aaaacttata cattattaaa catttgcgcg tcgattgact tttatttatt
6661 attaacaaag aagtaatacc aacctaatta caaatacttc gaagtgacta tcataagttt
6721 ccttatctag cgaaggcaac ttttgaactc cccagttgtt aatatgtatc attatacacg
6781 acccaatcaa acgcggggaa gtcaatgccg aaagaattct aggacctaaa agctgctcaa
6841 tccttgggcc tttccctaat gacatcccct ctc
//