GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS DROPG418 5073 bp ds-DNA INV 18-MAY-1991
DEFINITION Transposable P vector conferring G418 resistance in Drosophila
ACCESSION X01803
KEYWORDS inverted repeat; neomycin resistance; polylinker;
transposable element.
SOURCE Drosophila melanogaster DNA.
ORGANISM Drosophila melanogaster
Eukaryota; Animalia; Metazoa; Arthropoda; Uniramia; Insecta;
Pterygota; Neoptera; Holometabola; Diptera; Brachycera;
Cyclorrhapha; Schizophora; Drosophiloidea; Drosophilidae.
REFERENCE 1 (bases 1 to 5073)
AUTHORS Steller,H. and Pirrotta,V.
TITLE A transposable P vector that confers selectable G418 resistance to
Drosophila larvae
JOURNAL EMBO J. 4, 167-171 (1985)
STANDARD full automatic
COMMENT Data kindly reviewed (19-FEB-1986) by V. Pirrotta
From EMBL 26 entry ATPG418; dated 06-JUL-1989.
FEATURES Location/Qualifiers
transposon 1..542
/note="P element fragment"
repeat_unit 1..31
/note="inverted repeat"
misc_feature 543..3208
/note="pUC 8 vector"
misc_feature 705..740
/note="polylinker site"
promoter 3221..3672
/note="Drosophila promoter region hsp 70"
misc_feature 3676..4678
/note="bacterial neomycin resistance gene (neo)"
transposon 4685..5073
/note="P element fragment"
repeat_unit 5044..5073
/note="inverted repeat"
BASE COUNT 1317 a 1222 c 1287 g 1247 t
ORIGIN
1 catgatgaaa taacataagg tggtcccgtc gaaagccgaa gcttaccgaa gtatacactt
61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
541 aacgccattc gccattcagg ctacgcaact gttgggaagg gcgatcggtg cgggcctctt
601 cgctattacg ccagctggcg aaggggggat gtgctgcaag gcgattaagt tgggtaacgc
661 cagggttttc ccagtcagta cgttgtaaaa cgacggccag tgccaagctt ggctgcaggt
721 cgacggatcc ccgggaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
781 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
841 taatgagtga ggtaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
901 aacctgtcgt gccagctgga ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
961 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
1021 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
1081 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
1141 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
1201 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
1261 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
1321 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
1381 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
1441 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
1501 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
1561 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
1621 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
1681 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
1741 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttag
1801 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
1861 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
1921 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
1981 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
2041 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
2101 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
2161 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
2221 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
2281 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
2341 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
2401 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
2461 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
2521 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
2581 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
2641 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
2701 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
2761 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
2821 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
2881 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
2941 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
3001 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
3061 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
3121 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
3181 gcgtaaggag aaaataccgc atcaggcgaa ttaattcccc tagaatccca aaacaaactg
3241 gttattgtgg taggtcattt gtttggcaga agaaaactcg agaaatttct ctggccgtta
3301 ttcgttattc tctcttttct ttttgggtct ccctctctgc actaatgctc tctcactctg
3361 tcacacagta aacggcatac tgctctcgtt ggttcgagag agcgcgcctc gaatgttcgc
3421 gaaaagagcg ccggagtata aatagagcgc ttcgtctacg gagcgacaat tcaattcaaa
3481 caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca
3541 agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac
3601 caagtaaatc aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga
3661 gaactctgaa taggggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
3721 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
3781 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
3841 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
3901 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
3961 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
4021 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
4081 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
4141 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
4201 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
4261 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
4321 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
4381 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
4441 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
4501 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
4561 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
4621 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccga
4681 tccgtcgagc gtataaccat ctgtacaaaa aaggatttcc tttgcccagt cgtacgactt
4741 tgtacagatg gttatcagat gtggacataa aaagaggatg tttggatgtg gtcatagacc
4801 taatggacag tgatggagtt gatgacgccg acaagctttg cgtactcgct caaattatta
4861 aaaataaaac tttaaaaata atttcgtcta attaatatta tgagttaatt caaaccccac
4921 ggacatgcta agggttaatc aacaatcata tcgctgtctc actcagactc aatacgacac
4981 tcagaatact attcctttca ctcgcactta ttgcaagcat acgttaagtg gatgtctctt
5041 gccgacggga ccaccttatg ttatttcatc atg
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