GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOASPS 3868 bp ds-DNA BCT 18-MAY-1991
DEFINITION E. coli aspS gene for aspartyl-tRNA synthetase (EC 6.1.1.12)
ACCESSION X53863
KEYWORDS aminoacyl-trna synthetase; aspS gene.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 3868)
AUTHORS Eriani,G.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Eriani,G., Dirheimer,G. and Gangloff,J.
TITLE Aspartyl-tRNA synthetase from Escherichia coli: cloning and
characterization of the gene, homologies of its translated amino
acid sequence with asparaginyl- and lysyl-tRNA synthetases
JOURNAL Nucleic Acids Res. 18, 7109-7118 (1990)
STANDARD full staff_review
COMMENT *source: strain=K12; The gene product has an alpha-2 subunit
structure.
From EMBL entry ECASPS; dated 28-JAN-1991.
FEATURES Location/Qualifiers
-35_signal 1241..1245
-10_signal 1266..1271
prim_transcript 1278..3270
/gene="aspS"
RBS 1359..1363
mat_peptide 1372..3141
/EC_number="6.1.1.12"
/product="aspartate-tRNA ligase"
/gene="aspS"
CDS 1372..3144
/EC_number="6.1.1.12"
/product="aspartate-tRNA ligase"
/gene="aspS"
/codon_start=1372
BASE COUNT 947 a 973 c 1066 g 882 t
ORIGIN
1 aagcttatcg ggcggctgtc taaaatcacc cgcattaacg ccgcgctgat gtaaaccgcg
61 attaagcgtt tgttcctctt ctcctttggc gaaaaactgt ggatggcgga cttccacgcc
121 ataattaaat tcgccaggaa gagaatcgag aaaatgccaa agcgcaggca gctcccgtgg
181 gccgaatgtg gcaggcagtt gcagccagta ttgcccaatg cgcggagcca acggtgacat
241 gcgggtcaaa aattcagtca ctaaatcatc gcaatgccgt aatgctgcct gatgcgaaat
301 ggtcgccgga aacttaaaac agaagcggaa gtcatctgtg gtctcgtcac gccagcgcag
361 gacaacctcg ggtttcggca gggcgtaaag cgtggtgttg ccctccacgc agttaaagtg
421 gcgggcatac tcttcaaggc tggtgatccc caaccgcacc catttaggat gcgaccattg
481 cggtagaccg atgtaaatca taacgcgttg aggatctctt ccacgtacgc acacgggcga
541 tgcgcgggta gatatggttg atactgttat tgtgctgctc ggcgctggcg gcactacagg
601 cgtcttcggc aatcaccaga ttaaagccaa gttcccaggc attgcgggcg gtggattcaa
661 caccgatatt ggtcgagatc ccacataaca ctattgtatc gatacctcgg cggcgtaatt
721 gcaactccag atccgtaccg taaaacgcac cccattgacg tttgatgatt tcgatatcgc
781 tgtcggttgc acctaatgca gcaggatgtt gccaccaatt ttcgggcaac actttagccg
841 gtgagggcgc atcgaccggc tgttttaatg cttcagcgta atcggcagac cagccaacgc
901 gcaccagaaa cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat
961 taactacctc atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat
1021 cgatcaccac cagcgcagtg gttttagcat taagttcaag cataatgact cctgaaaaaa
1081 caaaatcgtg cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa
1141 tttttatgag ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc
1201 gatataaagt ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt
1261 tccagtataa tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg
1321 gtcacctgcg cagcaggggc atccgccggc agctaagtta agggatatct catgcgtaca
1381 gaatattgtg gacagctccg tttgtcccac gtggggcagc aggtgactct gtgtggttgg
1441 gtcaaccgtc gtcgtgatct tggtagcctg atcttcatcg atatgcgcga ccgcgaaggt
1501 atcgtgcagg tatttttcga tccggatcgt gcggacgcgt taaagctggc ctctgaactg
1561 cgtaatgagt tctgcattca ggtcacgggc accgtacgtg cgcgtgacga aaaaaatatt
1621 aaccgcgata tggcgaccgg cgaaatcgaa gtgctggcgt cctcgctgac tatcatcaac
1681 cgcgcagatg ttctgccgct tgactctaac cacgtcaaca ccgaagaagc gcgtctgaaa
1741 taccgctacc tcgacctgcg tcgtccggaa atggctcagc gcctgaaaac ccgcgctaaa
1801 atcaccagcc tggtgcgccg ttttatggat gaccacggct tcctcgacat cgaaactccg
1861 atgctgacca aagccacgcc ggaaggcgcg cgtgactacc tggtgccttc tcgtgtgcac
1921 aaaggtaaat tctacgcact gccgcaatcc ccgcagttgt tcaaacagct gctgatgatg
1981 tccggttttg accgttacta tcagatcgtt aaatgcttcc gtgacgaaga cctgcgtgct
2041 gaccgtcagc ctgaatttac tcagatcgat gtggaaactt ctttcatgac cgcgccgcaa
2101 gtgcgtgaag tgatggaagc gctggtgcgt catctgtggc tggaagtgaa gggtgtggat
2161 ctgggcgatt tcccggtaat gacctttgcg gaagcagaac gccgttatgg ttctgataaa
2221 ccggatctgc gtaacccgat ggaactgact gacgttgctg atctgctgaa atctgttgag
2281 tttgctgtat ttgcaggtcc ggcgaacgat ccgaaaggtc gcgtagcggc tctgcgcgtt
2341 ccgggcggcg catcgctgac ccgtaagcag atcgacgaat acggtaactt cgttaaaatc
2401 tacggcgcga aaggtctggc ttacatcaaa gttaacgaac gcgcgaaagg tctggaaggt
2461 atcaacagcc cggtagcgaa gttccttaat gcagaaatca tcgaagacat cctggatcgt
2521 actgccgcgc aagatggcga tatgattttc ttcggtgccg acaacaagaa aattgttgcc
2581 gacgcgatgg gtgcactgcg cctgaaagtg ggtaaagacc ttggtctgac cgacgaaagc
2641 aaatgggcac cgctgtgggt tatcgacttc ccgatgtttg aagacgacgg tgaaggcggc
2701 ctgacggcaa tgcaccatcc gttcacctca ccgaaagata tgacggctgc agaactgaaa
2761 gctgcaccgg aaaatgcggt ggcgaacgct tacgatatgg tcatcaatgg ttacgaagtg
2821 ggcggtggtt cagtacgtat ccataatggt gatatgcagc agacggtgtt tggtattctg
2881 ggtatcaacg aagaggaaca gcgcgagaaa ttcggcttcc tgctcgacgc tctgaaatac
2941 ggtactccgc cgcacgcagg tctggcattc ggtcttgacc gtctgaccat gctgctgacc
3001 ggcaccgaca atatccgtga cgttatcgcc ttcccgaaaa ccacggcggc agcgtgtctg
3061 atgactgaag caccgagctt tgctaacccg actgcactgg ctgagctgag cattcaggtt
3121 gtgaagaagg ctgagaataa ctgatatgac tcaaatacac gaaatcattc gcgttgcatc
3181 gaggcggcaa ctgagtgaac tcccatgagc atagataact atgtgaatgg gatgagcgaa
3241 ggcagtcaac gaagaggcag cgtgaaggat aaagtgtata agcgtcccgt ttcgatctta
3301 gtggtcatct acgcacaaga tacgaaacgg gtgctgatgt tgcagcggcg tgacgatccc
3361 gatttctggc agtcggtaac cggcagcgtg gaagagggtg aaaccgcgcc gcaagctgcc
3421 atgcgcgaag taaaggaaga ggtcaccatt gatgttgtcg ctgaacaact gaccttaatt
3481 gactgtcagc gcacggtaga gtttgaaatt ttttcacatt tacgtcatcg ctatgcgccg
3541 ggcgtgacgc gtaatacgga atcatggttc tgtcttgcgc ttccgcacga gcggcagatc
3601 gttttcactg aacatctggc ttacaagtgg cttgatgcgc ctgctgcggc ggcgtcacta
3661 agtcctggag caaccggcag cgattgaaca tttgtaatta acgctgcctg aacaggcagg
3721 gctatctgga gatattttta tggcaggtca tagtaaatgg gccaacacca gacatcgtaa
3781 agctgcgcag gatgctaagc gcggtaaaat cttcactaaa atcattcgtg agctggtaac
3841 cgcggctaag ctgggcgtgg cgatccgg
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