GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS PALHSP70 4470 bp ds-DNA INV 18-MAY-1991
DEFINITION Sea urchin hsp70 gene II for heat shock protein 70.
ACCESSION X16544
KEYWORDS heat shock protein; heat shock protein 70; hsp 70 gene.
SOURCE Paracentrotus lividus DNA.
ORGANISM Paracentrotus lividus
Eukaryota; Animalia; Eumetazoa; Echinodermata; Echinozoa;
Echinoidea; Echinacea; Echinoida; Echinidae.
REFERENCE 1 (bases 1 to 4470)
AUTHORS Sconzo,G.
JOURNAL Unpublished (1989)
STANDARD full automatic
REFERENCE 2 (bases 1 to 4470)
AUTHORS La,R.M., Sconzo,G., Giudice,G., Roccheri,M.C. and Di,C.M.
TITLE Sequence of a sea urchin hsp 70 gene and its 5' flanking region.
JOURNAL Gene 96, 295-300 (1990)
STANDARD full automatic
COMMENT *source: cell type=spermatozoa; library=EMBL3; clone=lambda PLH1;
*source: developmental stage=adult,germline; tissue=gonads;
*source: haplotype=1N;
From EMBL entry PLHSP70; dated 28-JAN-1991.
FEATURES Location/Qualifiers
mat_peptide join(:1272..1473,:1771..1977,:2533..2687,:3273..3551,
:3855..4127)
/partial
/product="heat shock protein 70"
/gene="hsp 70 gene II"
mRNA join(:<1272..1473,:1771..1977,:2533..2687,:3273..3551,
:3855..4127)
/partial
/gene="hsp 70 gene II"
exon <1272..1473
/gene="hsp 70 gene II"
CDS join(:1272..1473,:1771..1977,:2533..2687,:3273..3551,
:3855..4127)
/partial
/product="heat shock protein 70"
/gene="hsp 70 gene II"
/codon_start=1272
misc_feature 112..127
/note="Heat shock regulatory element HSE 4"
misc_feature 484..497
/note="Heat shock regulatory element HSE 3"
misc_feature 499..509
/note="Heat shock regulatory element HSE 2"
CAAT_signal 933..937
CAAT_signal 955..959
misc_feature 1009..1021
/note="Heat shock regulatory element HSE 1"
TATA_signal 1038..1044
intron 1474..1770
/number=1
exon 1771..1977
/gene="hsp 70 gene II"
intron 1978..2532
/number=2
exon 2533..2687
/gene="hsp 70 gene II"
intron 2688..3272
/number=3
exon 3273..3551
/gene="hsp 70 gene II"
intron 3552..3854
/number=4
exon 3855..4127
/gene="hsp 70 gene II"
intron 4128..>4470
/number=5
BASE COUNT 1389 a 854 c 913 g 1314 t
ORIGIN
1 ggatccctct ttctgatggg gatatcagat tttcaaaata ttaattactt tttgagatat
61 ccggcatccg tgaatttagg tcacgtgcga attatggtca caccaccata cctgtgaaca
121 ttctttgagg ctgctcagtg caaaaaatta atcgcgcaaa aatgacagcg aatacagact
181 tacagtactt acagatgcgg taagtggaat acggtaactt acatgtaatg gaacttatgt
241 tatggcaaca cttgtctttg caaaaaacgt acttgagtct gtacactgca gtttacacag
301 tgctcacatg catgctctct atcttcagag ggatattatg atgaagacag agacagcctc
361 ggcttatgcc cacgcatgtc ccagaccttg gaatggacct aagctccata tacatacagt
421 caaagttccc ccctgattcg acagtctact ggtacaaaaa catattccct atggcataaa
481 caatgtgaaa tttcggaaac gaatttcctc gtaaaacacc cctattatgt agtaaaatgg
541 tgatttaact ttaaacttaa gcagtctgga aggctgataa cggaccggtt tcgacgcttt
601 tgggggatgc tcatgatcat gtgcgtcccc acaaagtgtc gaaaagtatc atgtggtgag
661 tataaccttt ctgttttctg tctgccttga cctgacaaag gtaaggcctg gtccttggtt
721 tgctctaaac ctccagtgct cactctgcat gagcgcccat catgggtgga tacggtgctg
781 actacatgaa gccatcatca tcatcgactc atcatcattt acaccttata cttcatggac
841 ttgacctccg ccccaccatg tccatgaaga cttttctatg ctctgagaac tgaaggtcga
901 tctgaaaagt attctatatt atagtaactt tgccaataat ggtaactaca aattccaata
961 aaaaattgca aagccttgat atcaggaaat cttggaaact acttgagata tgaacttcaa
1021 acttgatgag aacactgtat attacgatgt aattcaggta gaggttaagg aggtctattt
1081 ctgattgact gcatggagca tatagtcgct atttgttttg tttcagatta gtagagttgt
1141 taatggtcga ctataacgtg ctggtctgcg ttaatcgcaa ccagcgcgca cgaaccgttg
1201 cagacaattc tccactcttt atttataata acataatcat actaattatt tctcattttc
1261 tgaaaagcaa aatggctaag gcaccagcag taggaattga ccttggaaca acatactcct
1321 gtgttggagt cttccagcat ggaaaagttg aaatcatcgc caatgaccaa ggaaacagaa
1381 ccactcccag ctatgtagcc ttcactgata cagaacgtct tattggagat gcagcaaaga
1441 acaagactgc tagtaaccca tacaggtctc ttggtaagtt agatgcagga gactgtattg
1501 ctcgttctcc aaggcatttg aatgactcta cgtcattata tgtatatttt ttatgcgctg
1561 tacatgtgga aaataaaaac aaaagtacct ttcttatcca aaagaagtgc caattaatga
1621 acatttcagt acactgttat gaaatgctga aaattactga ctttatttag gcttattgag
1681 tcaagctatg gagtaagatt ttaaggactg ctggtatatc acgcatgtga tttttaatat
1741 ggctcactaa gctatgaaat ttgtttccag atgccaagcg tctcatcggt cgtaatttca
1801 gtgacacgaa tgtgaaggct gacatgaaac attggccctt cacagtgata gaggaaggag
1861 gcagacctaa gatcaagatt gaattcaagg gagaaagtaa gactttctat gctgaggaga
1921 tcagctccat ggtattactc aagatgaagg aaactgcaga agcatacctt gggaaaggtt
1981 agtatttgag atctcagata aacttttata tcacaaatag tgtctatata atatgtgaat
2041 catcaatcaa atgagacaag tttcaagcaa gagtaaaaaa aaaaaaaaag ataagaaaat
2101 caccaaaaat gataaaaaat gtccaaaatt tgagggaaat acttatcatt tcaaaccttt
2161 aatgggtaaa acggcatgaa gtatttaaag aatttattat tttttctgtt ttcctggact
2221 ctttgctcag tgactgtgtc tcatttgtct ctatttgaat atgatatcca aactgtcaca
2281 caactctttt catggtctat tgcctagcac gatatatgat gactgcacga tctctcattg
2341 acaggttcca tggtataact agaaaactag ttcctcaagc tgtaacgaac atgctgtgtc
2401 taggtaaaaa aggaggataa aagagacaca tagtagattg tatgtaatgc taacactaag
2461 gaaaattaag tgtataagga acaattgaag ccaatttaac tttttccccc caatatttat
2521 ctttggttac agaaagtgtt acagatgccg ttgttactgt gcctgcctac ttcaacgact
2581 ctcagagaca ggcaacaaag gaatgcggtg tgatctctgg tatgaacatt ctacggatca
2641 tcaatgaacc tacggcagct gccattgctt atggtctgga caagaaggta attcttgtta
2701 aagaaaggaa aaagtaagga aaggtcatgt ttcacgttca ttaggtcttg caaagacaat
2761 cttacaaggg aaagttagag gccggccgca gacgaggaag acaaagaaaa ctgtgggaag
2821 ataacatccg agaatggacg gggatgagac tcccggagtc cataagagtg gtggaggaca
2881 aggcggttgg aggaagatgt tgtcccatat taatgtgcct cttcaaacac cagcaagtca
2941 ggaacgtact tacctacggc gaaactggcg gataaactgg gtggttgtcc acggactggc
3001 ttgagagaac gtgtaccgta ttccttatga ctatattcaa tccaatttaa tggactgtat
3061 caatcaatta aacgtagaat aatttaaagc acacattgaa aggatagtta attcacatca
3121 tggacttcca attttgctat gtttctttat taggcctatc agaattattt ggaatgattc
3181 tctgattaca gtcactttgt caacttaaga tatggctcca ttaatgtgaa tcatttctta
3241 ttacattcat gacgatgtaa ctctttcact agggtggtgc tgagcgcaat gtcctgatct
3301 ttgacttggt gggtggaacc ttcgatgtgt ctgtgctcac tatcgaggag gggatctttg
3361 aagtgaagtc gacttctcgc gacactcact tgggaggaga ggacttcgac aaccgtatgg
3421 tcacccattc atcaccagag ttcaagagga agcacaagaa ggacattact cctaacaagc
3481 gagcagtaag aagattgagg acagcttgtg aaagggcaaa gaggactcta tcatcaagca
3541 cacaggccaa ggtaagtttg cctaaaaatt gaaagatatg caatgaaaat aatggtcttg
3601 ggaaatgctg gtacccacca actcaaatgc gtatatttca atatttatca attttcagaa
3661 aattgtgtta ctaatgtata caaaaacgga tttaatatga tttttttttt ttttttgttt
3721 ttttttttgc tttctatcat ttgaaataat ttagatatga ttgcttttag tttatataaa
3781 agtcattgta tcattacatt tattttctat caagtgagga tattcattga ttttttttcc
3841 attgtatctt gcagattgag attgattctc tgtttgaggg catcgattac tacacctccg
3901 tcacccgtgc tcgatttgag gaactgaact ccgatctgtt ccgtggcacc cttgaaccgg
3961 ttgagaatgc ccttcgagat gccaagctgg ataaggaaaa gatccatgag attgtcttgg
4021 tcggaggctc taccagaatc cccaagatcc agaaactcca ggacttcttt cacggcaaag
4081 aactcaataa gagcatcaac cctgatgagg ccgttgctat cgtgcaggta tgcgtctagt
4141 tcatggaggc ctttcttcta atgtgcagcc ctgtttggta gtcaagaaaa gaaacccttt
4201 agcgcttgtt aatcctcttg aacccttggt gaagtctctg taaaaccctt aagaaatgca
4261 tgtcaacatg gaaatgaaat cttcttcagt gatacaaagc taaaaaattt atatgaatat
4321 caggaaatgt gtaaagaagc tgtaatacta catcttgtta ctgtactttc ttcaaaagtc
4381 tagagcagtc tagagattac tcccattata aagagcacag aaacaactgg cagttctccc
4441 agtcctgagt ccgttgacct gcaggtcgac
//