GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS PRMPVUII 1321 bp ds-DNA BCT 18-MAY-1991
DEFINITION Proteus vulgaris plasmid gene for PvuII restriction enzyme
ACCESSION X52681
KEYWORDS PvuII restriction enzyme; restriction enzyme.
SOURCE Proteus vulgaris DNA.
ORGANISM Proteus vulgaris
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1321)
AUTHORS Athanasiadis,A.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 1321)
AUTHORS Athanasiadis,A., Gregoriu,M., Thanos,D., Kokkinidis,M. and
Papamatheakis,J.
TITLE Complete nucleotide sequence of the PvuII restriction enzyme gene
from Proteus vulgaris
JOURNAL Nucleic Acids Res. 18, 6434-6434 (1990)
STANDARD full automatic
COMMENT *source: strain=ATCC 13315; Data kindly reviewed (12-DEC-1990) by
Athanasiadis A. Author's comment: all the elements in the sequence
are on the complementary strand, because this is the orientation of
te maps in the literature
From EMBL entry PVPVUII; dated 01-FEB-1991.
FEATURES Location/Qualifiers
CDS complement(526..996)
/note="PvuII restriction enzyme"
/codon_start=996
promoter complement(1031..1036)
/note="pot. TATA box"
promoter complement(1051..1056)
/note="pot. -35 region"
promoter complement(1167..1173)
/note="pot. TATA box"
promoter complement(1190..1195)
/note="pot. -35 region"
BASE COUNT 370 a 253 c 271 g 427 t
ORIGIN
1 tgaccgagac agaacaactc ttagagaacg ccttgaacac cttgttacaa cagcaagaga
61 ccgttatgca gcaatttatc tcagcgtgtc gggggaactc gagacacaga acagtcagtt
121 agccatgcaa tacagggaaa tcagtcaaca gtagcgactt taaccggact gctgaacagg
181 tatcagaacg gctgacacag cagatccagc gacaggagca gagtcgtagt cgggggtttc
241 agtatcggtt tttaacagga taaaaccgct tcaagccaca aacaagtttg cgactttcat
301 cggtttaggc gatcaaagtc ggacgaaaaa cggatgtaaa cagccctcaa acgtcacaga
361 aaccgttata aactttcagg atggatttgg gtgttatggg gggtgtttgg tgcttaaaat
421 cgtgctgatg cgttctgatg gtgactgacg ggcggtttta aggtgtaaac tcgcccgttt
481 aacataattg ggtcttatcg gaaccatgaa tgtagctcca atttagtaaa tctttgtccc
541 atgttccatt acatatttta caggtatttt agggttgtta atatctttat gcccatctga
601 ataccatttc ctttcccatt tatcatagta aaattctaga tcttttggct ctaatctgta
661 tatagcttct attgcgatac cacggtatat ggcaaaaatc caaggtactt gtctatattt
721 tgcaataatt acaggattca tgtggtggtg agttgaaaaa cctttagtga ggtctatgtt
781 tattgatttt aactcgtatt cttgtcctgc gttatctaca gcatcattac cttctcgtcc
841 tggtagtact gttaatcctg taattagaag gacttgaagc aactttccac cattatcttg
901 aaaaatatca tttattccat gttttaatgc taagtcttga tattcctgta tatgcggcca
961 aagctctaat aatttattta gatctgggtg actcattttc gtgttccatc attagtattg
1021 atatagaaac atttaaggca tttgctattc gctcaatgtt gtctatcgat atattccttt
1081 ccgctcgttc aattgaacca atgtaggttc tatggattcc cactagatca gctaagcttt
1141 cttgggatag acctagctcg cctcgcattt tttttacatt ctttgcaagg gttaatcggg
1201 ctgataaagg atttgtattt ctgctcatag acgggataat gatggtatga tgactttgaa
1261 tctacagact atgagtagca atgatatgtt gaattttgga aaaaaacctg cctacacaac
1321 t
//