GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS BACPHEST 3930 bp ds-DNA BCT 18-MAY-1991
DEFINITION B. subtilis pheS and pheT genes for phenylalanyl-tRNA synthetase
alpha and beta subunits
ACCESSION X53057
KEYWORDS pheS gene; pheT gene; phenylalanyl-tRNA synthetase;
phenylalanyl-tRNA synthetase alpha subunit;
phenylalanyl-tRNA synthetase beta subunit.
SOURCE Bacillus subtilis DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 3930)
AUTHORS Putzer,H.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 3930)
AUTHORS Brakhage,A.A., Wozny,M. and Putzer,H.
TITLE Structure and nucleotide sequence of the Bacillus subtilis
phenylalanyl-tRNA synthetase genes
JOURNAL Biochimie 72, 725-734 (1990)
STANDARD full automatic
COMMENT *source: strain=168; developmental stage=vegetative phase;
From EMBL entry BSPHEST; dated 07-FEB-1991.
FEATURES Location/Qualifiers
promoter 57..62
/note="-35 region"
promoter 80..85
/note="-10 region"
RBS 396..401
/note="ribosome binding site"
CDS 409..1437
/note="phenylalanyl-tRNA synthetase alpha subunit (AA
1-343)"
/codon_start=409
CDS 1456..3867
/note="phenylalanyl-tRNA synthetase beta subunit (AA
1-804)"
/codon_start=1456
terminator 3872..3905
/note="transcription terminator"
BASE COUNT 1172 a 818 c 1014 g 926 t
ORIGIN
1 tcactaaacg ttgcggttgc ggctgccatc ctcgtgtatc acttgcgagg atagtgttgc
61 gccaagcgtt ggatttctct ataatagaac ataatatttc aaaaaccgtg acagaggacc
121 tcaatttctg atcaactgct ttccagggag aaaatgccgc tgactgagag catttttaac
181 gcagccggaa attggcttca cctcttgagt tgtcgccggg accgcgtaca ctgcgcgtaa
241 aggtgaaccg gaatctgatt ccgttatgtc aatgaagctg acgacagcat cagtctgtct
301 gaaataaggg tggtaccgcg gccacaactc gtcccttgta caagggacgg gtttttttta
361 ttttccaaag acatatgacg gtttgtttac ataaaaggag gatgcaaaat ggaagaaaag
421 ctaaaacagc tggaacaaga agctttagaa caagtagaag cggcaagctc attgaaggtt
481 gtcaatgata ttcgggtgca atatctcgga aaaaaagggc cgattacaga agtgctgcgc
541 ggaatgggca agctttctgc tgaggaacgt ccaaaaatgg gggcgctcgc gaacgaagta
601 agggagcgta ttgccaatgc gattgctgac aaaaacgaga agcttgaaga agaggaaatg
661 aaacagaagc ttgcagacag acaattgacg tcacgctgcc gggaaccctg ttgcagtcgc
721 ggccgccatc cgctcactgt tgtcattgaa gaaattgaag atttatttat cggtatgggc
781 tacacagtcg aggaagggcc agaggttgaa acggattact acaacttcga atcgctcaat
841 cttccgaaag aacacccagc gcgcgatatg caggacagct tttacatcac agaggaaact
901 ttgatgagaa cgcaaacttc tcctgtccaa acacgtacga tggaaaagca tgaaggcaaa
961 ggtcccgtta aaatcatttg cccgggtaaa gtatatcgcc gtgataacga tgatgcgacg
1021 cactctcacc aatttatgca aattgaaggg cttgtcgttg acaaaaacat cagcatgagt
1081 gatttaaaag gaacgcttga acttgttgcg aaaaaaatgt tcgggcaaga ccgtgaaatc
1141 agactccgcc caagcttctt cccgtttact gagccttcag tagaagtgga tgtgacatgc
1201 tttaaatgcg gtgggaacgg ctgctcagta tgtaaaggaa caggctggat tgaaatcctc
1261 ggtgccggaa tggttcaccc gaacgtgctt aaaatggctg gctttgatcc gaaggaatat
1321 cagggcttcg cattcggaat gggtgttgag cgcatcgcga tgctgaaata tggcattgat
1381 gatatccgcc acttctatac aaacgatgtc agatttattt cgcagtttaa acaggcgtaa
1441 gaggaggatg caaggatgtt tgtttcttat aaatggttag aggattatgt tgatttaaaa
1501 ggcatggacc cggctgttct tgctgaaaaa attacaagag ccggtattga ggttgaagga
1561 attgaataca aaggagaagg catcaaaggc gttgtcatcg gccatgtgct ggagcgcgag
1621 cagcacccga atgctgataa gctgaataag tgccttgtgg atatcggagc tgaagcccct
1681 gtacaaatca tttgcggcgc gccgaatgtg gataagggac aaaaagtcgc agttgcaaca
1741 gtcggagcgg tgctgccggg caatttcaaa atcaaaaaag ccaacgttcg cggtgaagaa
1801 tcaaacggca tgatctgttc cttacaggag cttggtatcg aaagcaaact tgtggcgaag
1861 gagtatgcag aaggcatttt cgtattcccg aatgacgctg aaacaggaag cgatgcctta
1921 gcggctttac agcttgacga tgcgattctt gagctgggtt taacgccaaa ccgcgcggat
1981 gccatgaaca tgcttggtgt tgcttacgag gttgcggcga ttttagacac tgaggtaaag
2041 cttccgcaaa cggattaccc ggctgcttca gaacaagcgt ctgattacat ttcagtcaaa
2101 attgaagatc aagaagcgaa cccgctgtac actgcaaaaa tcattaaaaa tgtcacgatt
2161 gctccgtcac cgctttggat gcagacaaag ctgatgaatg caggcattcg tccgcacaac
2221 aacgtcgttg acatcacaaa ttttgtcctg ttggaatacg gacagccgct tcatgcgttt
2281 gattatgaca gatttggctc taaggaagtc gtagtaagaa aagcagctga gaatgaaatg
2341 atcgtaacac ttgatgatca agaacgtaag ctgtctgccg atcaccttgt cattacaaac
2401 ggaacgaaag cgcaggccgt tgccggtgtc atgggaggag cagagtcaga ggttcaggaa
2461 gatacgaaaa ccattttgct tgaggctgcg tatttcaacg ggcagaaggt tcgcaaggct
2521 tccaaagacc ttggtttgag aagtgaatca agcgtaagat ttgaaaaagg aattgatccg
2581 gcacgcgtac gtcttgcagc agaacgggct gcgcagctga tccatctgta tgccggcggt
2641 gaggtgcttg ctggaacggt tgaggaagat cacctgacaa tcgaagcaaa taacattcac
2701 gtatctgctg acaaagtgag cagcgttcta ggcctgacga tcagtaaaga agaactgatc
2761 agcatttata aacgtctcgg ttttacggtc ggtgaagcgg atgatcttct cgttgtgact
2821 gtcccatcac ggcgcggtga cattacaata gaagaggatt taatcgaaga agcggcaaga
2881 ctgtacggct acgataacat tccgtctacg cttcctgaga cagcgggaac tacaggcgga
2941 ttaacgccat atcaggcaaa acgccgtaaa gtcagacgct tccttgaagg cgcaggctta
3001 tcacaagcca tcacttactc actgacaaac gagaagaaag cgacagcgtt tgcgattgaa
3061 aaatcactga atactgtgct tgcgcttcca atgagtgaag aaagaagcat tctcagacac
3121 agtcttgttc caaatctgct tgattctgtt tcttacaatc ttgcgaggca gactgactca
3181 gttgcactgt acgaagttgg ctccgttttc ctaacgaagg aagaggacac aaaacctgtt
3241 gaaacagaac gcgtagcagg agctgttacc ggattatggc gcaagcagct atggcagggt
3301 gaaaagaaac cagttgattt ctttgttgtc aaaggaattg tggaagggct gttagacaaa
3361 ctcaacgtcc tagacagcat tgaatttgta cagtctgaac gcaaacagct gcatccgggc
3421 agaacagcga acattttatt gaatggttct ctgatcggct tcatcggcca agtccatcct
3481 tcattggaaa aagaactgga tataaaggaa acatacgtat ttgagctcga tcttcatgcg
3541 ttgctcgcag cagaaacagc accgcttgtt tacacggcga ttccaaaata tccgtctgtg
3601 acacgtgata tcgctcttgt aacagataaa accgtcacaa gcggacagct cgagagcgtc
3661 attaaagagg ccgggggcaa gcttctgaaa gaggtaaccg tatttgacgt atacgaagga
3721 gagcatatgg aggaaggcaa gaagtcagtc gctttctcac tccagtatgt caatcctgaa
3781 caaacactga ctgaagaaga agtgacaaag gcgcacagca aagtgctgaa agcgttagaa
3841 gacacatatc aagctgtttt aagaggctga taaaaaagct tgcagagaaa tctgcaagct
3901 tttttctatg aacgtttttt atcagcaagg
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