GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOAROCG 1690 bp ds-DNA BCT 18-MAY-1991
DEFINITION E. coli aroC gene for chorismate synthase (EC 4.6.1.4).
ACCESSION Y00720
KEYWORDS chorismate synthase.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1690)
AUTHORS White,P.J., Millar,G. and Coggins,J.R.
TITLE The overexpression, purification and complete amino acid sequence
of chorismate synthase from Escherichia coli K12 and its comparison
with the enzyme from Neurospora crassa
JOURNAL Biochem. J. 251, 313-322 (1988)
STANDARD full automatic
COMMENT *source: strain=K12 AB2849/pGM602;
From EMBL entry ECAROCG; dated 08-FEB-1991.
FEATURES Location/Qualifiers
prim_transcript <492..>1562
/gene="aroC"
/evidence=EXPERIMENTAL
RBS 483..488
/note="putative"
mat_peptide 492..1559
/EC_number="4.6.1.4"
/note="calculated M(r)=38183"
/product="chorismate synthase"
/gene="aroC"
/evidence=EXPERIMENTAL
CDS 492..1562
/EC_number="4.6.1.4"
/product="chorismate synthase"
/gene="aroC"
/codon_start=492
repeat_region 1600..1628
/rpt_type=inverted
/note="putative"
BASE COUNT 403 a 464 c 469 g 354 t
ORIGIN
1 gtcgacgcgg tggatatctc tccagacgcg ctggcggttg ctgaacagaa catcgaagaa
61 cacggtctga tccacaacgt cattccgatt cgttccgatc tgttccgcga cttgccgaaa
121 gtgcagtacg acctgattgt cactaacccg ccgtatgtcg atgcgaagat atgtccgacc
181 tgccaaacaa taccgccacg agccggaact gggcctggca tctggcactg acggcctgaa
241 actgacgcgt cgcattctcg gtaacgcggc agattacctt gctgatgatg gcgtgttgat
301 ttgtgaagtc ggcaacagca tggtacatct tatggaacaa tatccggatg ttccgttcac
361 ctggctggag tttgataacg gcggcgatgg tgtgtttatg ctcaccaaag agcagcttat
421 tgccgcacga gaacatttcg cgatttataa agattaagta aacacgcaaa cacaacaata
481 acggagccgt gatggctgga aacacaattg gacaactctt tcgcgtaacc accttcggcg
541 aatcgcacgg gctggcgctc ggctgcatcg tcgatggtgt tccgccaggc attccgctga
601 cggaagcgga cctgcaacat gacctcgacc gtcgtcgccc tgggacatcg cgctatacca
661 cccagcgccg cgagccggat caggtcaaaa ttctctccgg tgtttttgaa ggcgttacta
721 ccggcaccag cattggcttg ttgatcgaaa acactgacca gcgctctcag gattacagtg
781 cgattaagga cgttttccgt ccaggccatg ccgattacac ctacgaacaa aaatacggtc
841 tgcgcgatta tcgcggcggt ggacgttctt ccgcccgcga aaccgccatg cgcgtggcgg
901 caggagctat tgccaaaaaa tatctcgccg agaaatttgg tattgaaatc cgtggctgcc
961 tgacccagat gggcgacatt ccgctggata tcaaagactg gtcgcaggtc gagcaaaatc
1021 cgtttttttg cccggacccc gacaaaatcg acgcgttaga cgagttgatg cgtgcgctga
1081 aaaaagaggg cgactccatc ggcgctaaag tcaccgttgt tgccagtggc gttcctgccg
1141 gacttggcga gccggtcttt gaccgcctgg atgctgacat cgcccatgcg ctgatgagca
1201 tcaacgcggt gaaaggcgtg gaaattggcg acggctttga cgtggtggcg ctgcgcggca
1261 gccagaaccg cgatgaaatc accaaagacg gtttccagag caaccatgcg ggcggcattc
1321 tcggcggtat cagcagcggg cagcaaatca ttgcccatat ggcgctgaaa ccgacctcca
1381 gcattaccgt gccgggtcgt accattaacc gctttggcga agaagttgag atgatcacca
1441 aaggccgtca cgatccctgt gtcgggatcc gcgcagtgcc gatcgcagaa gcgaatgctg
1501 gcgatcgttt taatggatca cctgttacgg caacgggcgc aaaatgccga tgtgaagact
1561 gatattccac gctggtaaaa aatgaataaa accgcgattg cgctgctggc tctgcttgcc
1621 agtagcgcca gcctggcagc gacgccgtgg caaaaaataa cccaacctgt gccgggtagc
1681 gccaaatcga
//