GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOIICAT 930 bp ds-DNA BCT 18-MAY-1991
DEFINITION E. coli II-CAT gene for chloramphenicol acetyltransferase (EC
2.3.1.28)
ACCESSION X53796
KEYWORDS II-CAT gene; chloramphenicol acetyltransferase;
chloramphenicol resistance.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 930)
AUTHORS Murray,I.A.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Murray,I.A., Martinez-Suarez,J.V., Close,T.J. and Shaw,W.V.
TITLE Nucleotide sequence of genes encoding the type II chloramphenicol
acetyltransferase of Escherichia coli and Haemophilus influenzae,
which are sensitive to inhibition by thiol-reactive reagents
JOURNAL Biochem. J. 272, 505-510 (1990)
STANDARD full staff_review
COMMENT *source: strain=K12
From EMBL entry ECIICAT; dated 08-FEB-1991.
FEATURES Location/Qualifiers
mRNA <187..>828
/gene="II-CAT"
-35_signal 124..129
/note="putative"
-10_signal 150..156
/note="putative"
RBS 174..179
mat_peptide 187..825
/product="chloramphenicol acetyltransferase"
/gene="II-CAT"
CDS 187..828
/product="chloramphenicol acetyltransferase"
/gene="II-CAT"
/codon_start=187
BASE COUNT 255 a 190 c 199 g 286 t
ORIGIN
1 ggatccgcta tgtgtttgcg gatgattggc cggaataaat aaagccgggc ttaatacaga
61 ttaagcccgt ataggtatta ttactgaata ccaaacagct tacggaggac ggaatgttac
121 ccattgagac aaccagactg ccttctgatt attaatattt ttcactatta atcagaagga
181 ataaccatga attttacccg gattgacctg aatacctgga atcgcaggga acactttgcc
241 ctttatcgtc agcagattaa atgcggattc agcctgacca ccaaactcga tattaccgct
301 ttgcgtaccg cactggcgga gacaggttat aagttttatc cgctgatgat ttacctgatc
361 tcccgggctg ttaatcagtt tccggagttc cggatggcac tgaaagacaa tgaacttatt
421 tactgggacc agtcagaccc ggtctttact gtctttcata aagaaaccga aacattctct
481 gcactgtcct gccgttattt tccggatctc agtgagttta tggcaggtta taatgcggta
541 acggcagaat atcagcatga taccagattg tttccgcagg gaaatttacc ggagaatcac
601 ctgaatatat catcattacc gtgggtgagt tttgacggat ttaacctgaa catcaccgga
661 aatgatgatt attttgcccc ggtttttacg atggcaaagt ttcagcagga aggtgaccgc
721 gtattattac ctgtttctgt acaggttcat catgcagtat gtgatggctt tcatgcagca
781 cggtttatta atacacttca gctgatgtgt gataacatac tgaaataaat taattaattc
841 tgtatttaag ccaccgtatc cggcagaatg gtggcttttt tttatatttt aaccgtaatc
901 tgtaatttcg tttcagactg gttcaggatc
//