GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS YSCUBA1G 4795 bp ds-DNA PLN 18-MAY-1991
DEFINITION Yeast UBA1 gene for ubiquitin-activating enzyme
ACCESSION X55386 X56507
KEYWORDS UBA1 gene; ubiquitin; ubiquitn-activating enzyme.
SOURCE Saccharomyces cerevisiae DNA.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 4795)
AUTHORS Mcgrath,J.P.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (sites)
AUTHORS Mcgrath,J.P., Jentsch,S. and Varshavsky,A.
TITLE UBA1:an essential yeast gene encoding ubiquitin-activating enzyme
JOURNAL EMBO J. 10, 227-236 (1991)
STANDARD full staff_review
COMMENT *source: strain=S288C; library=lambda-gt11; **map:chromosome=XI.
From EMBL entry SCUBA1G; dated 12-FEB-1991.
FEATURES Location/Qualifiers
CDS 586..3660
/product="ubiquitin-activating enzyme"
/gene="UBA1"
/codon_start=586
BASE COUNT 1478 a 878 c 984 g 1455 t
ORIGIN
1 ggagagtata aaaggagaaa atacaaaatg tagttgtgtt gttacgcatc tcaaagaaga
61 aagaagaaga agaagaagag gacgtcgcac ttgcaagcaa aaacagcgac tggcgcgaga
121 acaacaaaca ctaatgcatg tctctgtcca gcaggaaaag cgagccttag tgtttcttat
181 tttctcgttc gcctcactag tttgtagtag ggtgggagaa acgatggagt ggattgtatg
241 atgaggaacc agatagggac aacagattct caagtgacga ggaacatctt ttaaagccca
301 gtttttagta gagcttaggg cgcctttact gactatccac tccttgttaa gctatgatct
361 tgtattcatg tgattgtaaa taatattaga gctctttctt gaattgccat tacgcttcca
421 tgcggcatat tctcgtgtag tgtgacaaaa ggtgcgggtg gcaaataaag gtaaaagaaa
481 atgatcaaaa agggccttta agtaggatga gcaaccgcca atcgctggat ataagcaaac
541 aaaaagtaag attagtagca aagcaaagaa catataacta tagctatgag cagcaataat
601 agtggtctct ctgccgccgg agaaatcgat gaaagtcttt attctcgtca actttatgtg
661 ttgggtaagg aagcaatgtt gaaaatgcaa acctccaacg tattaattct cggcttgaag
721 gggctaggtg ttgagatagc gaagaacgtt gtgttggcag gtgtcaaatc tatgaccgtt
781 tttgaccctg agccggttca attggcagac ttatcaaccc aattcttttt gaccgaaaag
841 gatattggtc aaaagagagg agatgtgacc agagccaaat tggcagagtt gaacgcatat
901 gtcccggtta atgtgctgga ttccttagac gatgttaccc aattgagtca attccaggtc
961 gttgtcgcta cggacactgt ttctttggaa gataaggtga aaatcaacga attttgccat
1021 tcctccggta ttagattcat ttcctcggag acaagagggc tgtttggtaa caccttcgta
1081 gacctaggtg acgagttcac agtgttggac ccaacgggtg aagaacctcg cactggtatg
1141 gtttcagaca tcgagcccga tggaacagtg accatgctag atgataacag acacgggttg
1201 gaagacggaa attttgtcag gttctccgaa gtagaaggtc ttgataagtt gaatgatggg
1261 acccttttta aagttgaggt tttggggccc tttgcattca gaattggttc agtcaaagaa
1321 tatggcgagt ataagaaggg cggtatcttc accgaagtca aagtaccccg taaaatctca
1381 ttcaagtctt tgaaacaaca actgtccaat cctgagtttg ttttttcgga ttttgctaaa
1441 tttgacagag ctgctcaatt gcatttggga tttcaggctt tacatcagtt tgccgttaga
1501 cataatggtg aactgccaag aacaatgaat gacgaagacg ccaatgaatt aatcaagttg
1561 gtcaccgatt tgtccgttca gcaaccggag gttctgggtg aaggtgtaga tgttaatgaa
1621 gatttaatca aagagctttc ctatcaggca agaggtgaca taccgggtgt cgtagcattt
1681 ttcggtggcc ttgtggcaca agaagtgtta aaagcatgtt ctggtaaatt tactccgcta
1741 aagcaattta tgtactttga ttccttggaa tcattgccag atcctaaaaa cttcccaaga
1801 aatgagaaga ctactcagcc agtaaactct cgttacgata accaaatcgc ggttttcggt
1861 ttggattttc agaaaaagat tgccaattca aaggtctttc ttgtcggatc tggtgccatt
1921 ggttgtgaga tgttgaaaaa ttgggcactt ttggggcttg gttctggctc cgacggttac
1981 atcgttgtta cggataacga ttcgatcgaa aagtccaact taaaccgtca gttcttgttt
2041 agaccaaagg atgtcggaaa gaacaaatct gaagtagcgg cagaggccgt ttgtgctatg
2101 aaccccgatt tgaagggcaa gataaatgct aaaattgata aagtggggcc agaaactgag
2161 aaaatattta atgactcctt ttgggaaagc ctggattttg tcaccaacgc tctagacaat
2221 gtcgacgcaa gaacatacgt agatcgccgt tgtgtgtttt atagaaaacc actgttagaa
2281 tctggtaccc taggtactaa aggtaatact caagtcatca ttccaagatt gactgaatca
2341 tactcttctt ctagagaccc accagaaaag tctatcccat tgtgtaccct acgttctttc
2401 ccaaacaaga ttgatcacac cattgcctgg gccaaatctc ttttccaagg ttacttcact
2461 gattccgccg aaaacgttaa tatgtacttg acacaaccga atttcgttga gcaaacacta
2521 aagcaaagcg gggatgtcaa gggagttcta gaatccatct cagattctct atctagtaaa
2581 ccacacaatt ttgaagattg tatcaaatgg gcaaggttag aatttgaaaa aaaattcaac
2641 catgatatca agcagttact gttcaacttc cccaaggatg ccaagacttc caacggtgaa
2701 ccattttggt ccggtgctaa gcgtgctcca acaccattag agtttgatat ttacaataac
2761 gatcacttcc attttgtcgt agcaggtgct aatttacgcg cttataatta tggtattaaa
2821 agtgatgact ctaattctaa acccaacgtg gacgagtata agtccgtcat cgatcatatg
2881 ataattccag aatttactcc aaatgccaat ttgaaaattc aagttaatga cgatgatccg
2941 gatccaaatg ccaacgcggc taatggctca gatgaaattg atcagctggt atcatcccta
3001 ccagatcctt ctactttggc tggctttaaa ctggagcctg tagatttcga gaaagacgat
3061 gacactaatc atcatattga atttataacg gcttgttcta actgtagagc tcaaaactac
3121 ttcatcgaaa ctgctgatcg ccaaaagacc aaattcatag ctggccgtat tattcctgcc
3181 atcgcaacaa ccacttcttt ggttactggt ttggtcaatt tggaattata taaactgatt
3241 gacaacaaga cagatattga gcaatataag aatggctttg ttaatttagc tttgccattt
3301 ttcggttttt cggaaccaat tgcttcacca aagggagaat ataacaacaa gaaatatgat
3361 aaaatttggg atagatttga tatcaaggga gatattaaat taagtgattt aattgaacat
3421 tttgaaaaag atgaagggtt agaaataaca atgctttctt atggcgtttc tcttctttat
3481 gcctcatttt tccctccaaa gaaattgaaa gaaagactaa atctaccaat aactcaattg
3541 gttaaattag tgactaagaa agatattccg gcacatgtat ctacaatgat tctcgaaatt
3601 tgcgcagatg acaaggaagg agaggatgtt gaagttcctt tcattaccat tcatctatga
3661 gtaactaatt aaccaaaata aaaaaataaa ttaaatatat acattaaata tgtatatata
3721 tatgtatgac ttttgacaac tttttctttg gtaaaaactt attattatta ttattattat
3781 tattattatt aatgtagtct cgaatatttg agtatgtttt agttttttgt tttatatttt
3841 ctttgggttt aactgctttg gttggaaaca atttggaata attccccgcg attattatat
3901 aaagtgtcga agttacctcg ctcaaccact ttaccatctt taagaactgc aatcgagtta
3961 caagacctca tcatttgttc actatgcgtt ataaccattg ttagtagagc aggtggacct
4021 tttttgacga tctcattgat gatagaggag ctgacagaat ccaaggctga agtacactca
4081 tctaaaatca gaatttttga ttttctcaga agtgctctgg ctatgcaaag cctttgcgct
4141 tgtccacctg acagtaaagt tgtatcaata cgtgtatcca agccctgagg tgatgaaatt
4201 acaaagtcat ggattcctac gtattttaat gcatcataca tttcaatttc aagtatttca
4261 tcttgtaaac cataagttag gttatctctg atggttccat tgaataataa aggtttttgc
4321 tcaaccactg aaatttcttt tcttaaactt gtcaaattcc agtcatttac gtccgtaccg
4381 tctattttaa ttttgcctac ttcacaatta taaagttttg ttaataaaag cacaagtgta
4441 gactttcctg tgcctgattc accaatgata cctaacgtct gtccgcaaaa catgtcaaaa
4501 ttcatgtttt tgtaaacaaa ggcggtaggt gcagatggat aggcaaatgt caaattttga
4561 attgaaacga ttggtttttt ttctttgcca tggtaggtgt gaccagctat tcccactgtt
4621 ctagcattat tgttttcaac ctctagggta ttatgctttt catcaagaat cctatagatc
4681 caactggcag cacgttggcc tctacttata tcgggtatct gacttactag gctagtgcat
4741 gacataatag tgaataataa caaagtgaaa gtcgtaaaca tttcctttga ggtgt
//