GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS ECOTN5X 1300 bp ds-DNA BCT 18-MAY-1991
DEFINITION Transposon Tn5 fragment encoding neomycin and kanamycin resistance
(neo) and a fragment of the reading frame of a further protein.
ACCESSION V00618
KEYWORDS drug resistance gene; neomycin resistance; transferase.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1300)
AUTHORS Beck,E., Ludwig,G., Auerswald,E.A., Reiss,B. and Schaller,H.
TITLE Nucleotide sequence and exact localization of the neomycin
phosphotransferase gene from transposon Tn5
JOURNAL Gene 19, 327-336 (1982)
STANDARD full automatic
COMMENT From EMBL entry ISTN5X; dated 13-FEB-1991.
FEATURES Location/Qualifiers
CDS 151..942
/note="neomycin phosphotransferase"
/codon_start=151
CDS 942..>1300
/note="unidentified reading frame"
/codon_start=942
BASE COUNT 245 a 374 c 408 g 273 t
ORIGIN
1 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
61 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct
121 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
181 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
241 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
301 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
361 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
421 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
481 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
541 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
601 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
661 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
721 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
781 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
841 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
901 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
961 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
1021 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
1081 agcgcgggga tctcatgctg gagttcttcg cccaccccgg gctcgatccc ctcgcgagtt
1141 ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt ctaccggcag tgcaaatccg
1201 tcggcatcca ggaaaccagc agcggctatc cgcgcatcca tgcccccgaa ctgcaggagt
1261 ggggaggcac gatggccgct ttggtcgacc cggacgggac
//