GenBank-Updates@genbank.bio.net (05/18/91)
LOCUS CREGPLP 2314 bp ds-DNA PLN 18-MAY-1991
DEFINITION C. reinhardtii Cblp gene for a G protein beta subunit-like
polypeptide
ACCESSION X53574
KEYWORDS G-protein associated.
SOURCE Chlamydomonas reinhardtii DNA.
ORGANISM Chlamydomonas reinhardtii
Eukaryota; Plantae; Thallobionta; Chlorophycota; Chlorophyceae;
Volvocales; Chlamydomonadaceae.
REFERENCE 1 (bases 1 to 2314)
AUTHORS Schloss,J.A.
JOURNAL Unpublished (1990)
STANDARD full automatic
REFERENCE 2 (bases 1 to 2314)
AUTHORS Schloss,J.A.
TITLE A Chlamydomonas gene encodes a G protein beta subunit-like
polypeptide
JOURNAL Mol. Gen. Genet. 221, 443-452 (1990)
STANDARD full automatic
COMMENT cDNA clones were also sequenced to confirm the exon sequences and
to define the exon boundaries (cDNA clone=pcf8-13 from a pBR322
library).
From EMBL entry CRGPLP; dated 14-FEB-1991.
FEATURES Location/Qualifiers
mat_peptide join(:241..350,:578..827,:1063..1233,:1383..1645,
:1876..2035)
/product="putative protein has homology to G protein beta
subunit"
/gene="Cblp"
mRNA join(:215..350,:578..827,:1063..1233,:1383..1645,
:1876..2233)
/gene="Cblp"
/evidence=EXPERIMENTAL
CDS join(:241..350,:578..827,:1063..1233,:1383..1645,
:1876..2038)
/product="putative protein has homology to G protein beta
subunit"
/gene="Cblp"
/codon_start=241
exon 215..350
/gene="Cblp"
prim_transcript 215..>2233
/gene="Cblp"
intron 351..577
/number=1
exon 578..827
/gene="Cblp"
intron 828..1062
/number=2
exon 1063..1233
/gene="Cblp"
intron 1234..1382
/number=3
exon 1383..1645
/gene="Cblp"
intron 1646..1875
/number=4
exon 1876..2233
/gene="Cblp"
repeat_unit 2063..2081
/function="may form secondary structures to affect mRNA
stability"
/rpt_type=inverted
repeat_unit 2115..2135
/function="may form secondary structures to affect mRNA
stability"
/rpt_type=inverted
polyA_signal 2212..2216
/note="Chlamydomonas putative poly(A) signal"
polyA_site (2232.2233)..(2232.2233)
BASE COUNT 409 a 741 c 722 g 442 t
ORIGIN
1 gccagtctgg gctgcgtcgt gtgatgggtt cgtgccacgg aagctccgag cgcgtgacac
61 ctttcccagc taggcagaga ctacacagtc tggagcatgg aatgcttaca tctttatctg
121 cgggccccag aaagtttgcc gctttcatcg ccatcgcggg ccccatgcac ttgcgcgcac
181 cgcgtttagt cgcccgcccc acccacagtc ctccctcttt ctccaaacaa aagaagcaaa
241 atggccgaga ctctgactct gcgcgccacc ctgaagggcc acaccaactg ggtgacggcc
301 atcgccaccc ctctggaccc ctcgtccaac accctcctgt ccgcttcgcg gtgcgtccaa
361 ctaggcctca aagtagtctt cttcaccagg cagtgaagca tagaaaacag cgccaaaaga
421 accagccgcc gcatttctgc ccgcgcctac gcccatcgcc ggctatcggc tgccgcgtta
481 ttgctgcaat cagtgcacgt gttgtagcgc tggatcgctg gagctgagct gttctggagc
541 gttcttgctg aaattgccct tctctccttg cccgcagcga caagagcgtg ctggtctggg
601 agctggagcg ctcggagtcc aactacggct acgcccgtaa ggctctgcgc ggccacagcc
661 acttcgtgca ggacgtggtc atctcctccg atggccagtt ctgcctgact ggctcgtggg
721 acggcaccct gcgtctgtgg gacctgaaca ccggcaccac cacccgccgc ttcgtgggcc
781 acaccaagga tgtgctgtcc gtggctttct cggtggacaa ccgccaggtg gggcggcaag
841 gccaaggcga aggagggggg aggtcggcag gcggggtagc gcgtggatgc ccggcggagg
901 aaaccaccgc ggtgccagtg tgctgatgtg taggggttgc catgagctca tacctggctg
961 catgcttcac cacaggatta cttccccaga ggattactgc cttccacagt ggacatgccc
1021 tgacgctttc cggccctccg gacctcccgc aaacgcccac agatcgtgtc gggctcgcgc
1081 gacaagacca tcaagctgtg gaacaccctg ggcgagtgca agtacaccat tggcgagccc
1141 gagggccaca ccgagtgggt gtcgtgcgtg cgcttctcgc ccatgaccac caaccccatc
1201 atcgtgtccg gcggctggga caagatggtc aaggtgggtg tgcgctgtgc aaaggcccgc
1261 gtgccatact gttagggcag ctgttccttc ctgctttaag gatgtgcacg cggtttaccc
1321 acgcaacgaa tggagtagtc gacgaatcac tgacgttgct tgctgtggta tgtcgtgcgc
1381 aggtctggaa cctgaccaac tgcaagctga agaacaacct ggtgggccac cacggctacg
1441 tcaacaccgt gaccgtctcc cccgacggct cgctgtgcgc ctcgggcggc aaggacggca
1501 ttgccatgct gtgggacctg gctgagggca agcgcctgta cagcctggac gccggcgacg
1561 tcatccactg cctgtgcttc tcgcccaacc gctactggct gtgcgccgcc acccagtcct
1621 ccatcaagat ctgggacctg gagaggtgag acgcgcagga tggctggctg gcgggcaggg
1681 gagtgggagc acaggggtag gggcggggga acgcgatcgg gaccgggagg ggttactcgg
1741 cttgagatct ggccgagcgc agggggccgc gccgatcacg atacgggtat atcgaagcca
1801 ccccgctgtc gtctttccat caacacacat ttcctgacct ccacatcttc tcctcccttt
1861 tccctcgcac cacagcaaga gcatcgtgga cgacctgcgc cccgagttca acatcaccag
1921 caagaaggcc caggtgccct actgcgtgtc gctggcctgg tcggccgacg gctccaccct
1981 gtacagcggc tacaccgacg gccagatccg cgtctgggcc gtgggccact ccctgtaaat
2041 gcccagccgc gcctagctgc ggcctggccc tgcgggccag gagcatcggg agctggaggg
2101 gtttaggagg aggcgcgcag cggcggcggc tgcgccacct gttgagaggc gggcggcgtg
2161 ctgcgtcgcg gtgtgttgag gatgcgtcgc ggggctgccg gactggcgat ctgtaacgcg
2221 ttgatgctgg tcagtctcat ttcacgtgtt ctcccacgtt ctctcttgcg gaatgtaagg
2281 tgtgcgtgat ggcggctacc aagggaggcc tttc
//